Construction and validation of a necroptosis-related lncRNA signature for predicting the prognosis of gastrointestinal cancer patients.

Background: Given the high incidence and mortality rates of gastrointestinal (GI) cancer, along with the lack of effective prognostic markers, this study aimed to construct a prognostic signature to identify high-risk patients facilitate precision medicine, and ultimately improve patient outcomes.

Methods: We analyzed transcriptomic data for COAD, ESCA, READ, and STAD from the TCGA and GTEx databases. Using co-expression analysis, Cox regression, and least absolute shrinkage and selection operator (LASSO) regression, we developed a necroptosis-related lncRNA signature, termed the Necro-lnc score.

Contact-dominated localized electric-displacement-field-enhanced pressure sensing.

Pressure sensors, especially the typical capacitive sensors that feature low power consumption, have drawn considerable interest in emerging and rapidly growing fields such as flexible electronics and humanoid robots, but often suffer from limited performance. Here, we report a contact-dominated design for capacitive pressure sensors to dramatically improve the sensing response and linearity over a broad pressure range. This design is implemented by utilizing hierarchical microstructured electrodes made of robust conductive composites with metallic coverage and layered dielectrics with high unit-area capacitance to realize localized electric-displacement-field-enhanced capacitance change.

Positive association between hobby participation and objective and subjective cognition among adults aged 50 years and over in 24 countries.

Objectives: An aging population presents significant challenges to cognitive health. Although previous studies have linked hobby participation to improved cognitive health, most focus on specific regions or types of hobbies, lacking cross-cultural exploration. This study examines global trends in hobby participation and their relationship to cognitive function.

A new perspective on betaine: Targeting the glycine binding site of the NMDA receptor.

Betaine, a methylated glycine derivative, shows promise in treating neuropsychiatric and neurological disorders, but its mechanisms remain unclear. This study investigates betaine's concentration-dependent effects on NMDA receptor-mediated excitatory field potentials (EFPs) in mouse medial prefrontal cortex slices and calcium influx in cultured cortical neurons. We further assessed subtype specificity using HEK293 cells stably expressing GluN1/GluN2A, GluN1/GluN2B, or GluN1/GluN2C NMDA receptor subtypes, measuring calcium flux and single-channel activity.

Ionotropic Receptor Genes in Fig Wasps: Evolutionary Insights from Comparative Studies.

The mechanisms of chemoreception in fig wasps (Hymenoptera, Agaonidae) are of primary importance in their co-evolutionary relationship with the fig trees they pollinate. As the supplementary receptors to odorant receptors (ORs) and gustatory receptors (GRs) in insects, we compare the evolutionary characters of ionotropic receptors (IRs) among 25 fig wasp taxa in six genera. In total, we identified 205 IRs in 25 fig wasps, with each taxon recording from 5 to 12 IR genes.

Harnessing NKG2D CAR-T cells with radiotherapy: a novel approach for esophageal squamous cell carcinoma treatment.

Background: Esophageal squamous cell carcinoma (ESCC) represents a highly aggressive malignancy with poor prognosis and limited therapeutic advancements. While chimeric antigen receptor (CAR)-T-cell therapy has revolutionized cancer treatment, its application in ESCC remains poorly explored. This study pioneers the exploration of natural killer group 2 member D (NKG2D) CAR-T cells combined with radiotherapy for treating ESCC, with the goals of establishing a novel treatment strategy and achieving superior tumor control through combined immunoradiotherapy.

ER stress-related mitochondrial protein-coding gene risk model and in vitro experiments unveil OMA1 as a novel prognostic and therapeutic biomarker for low-grade glioma.

Background: Low-grade gliomas (LGG) are known for their slow growth yet retain the potential to progress to more aggressive malignancies. Glioma cells are frequently exposed to stressors such as hypoxia, nutrient deprivation, and oxidative stress, which disrupt protein folding within the endoplasmic reticulum (ER), leading to ER stress and activation of the unfolded protein response (UPR). ER stress plays a complex role in glioma initiation, progression, and resistance to chemotherapy.

Transient asymmetry during elastic snap-through: The interplay between imperfections and oscillations.

A symmetrically buckled arch whose boundaries are clamped at an angle has two stable equilibria: an inverted and a natural state. When the distance between the clamps is increased (i.e.

Capturing structural intermediates in an animal-like cryptochrome photoreceptor by time-resolved crystallography.

Animal-like cryptochromes are photoreceptors that control circadian rhythm and signaling in many eukaryotes. Transient photoreduction of the cryptochrome flavin chromophore initiated signaling via a poorly understood mechanism. By serial femtosecond crystallography (SFX), we show that the photoreduction mechanism of cryptochrome involves three loci [carboxyl-terminal region, a transient protonation pathway, and flavin adenine dinucleotide (FAD)-binding site] acting in unison to accomplish three effects: radical pair stabilization, protonation of FAD radical, and formation of the signaling state.

Achieving symmetric snap-through buckling via designed magnetic actuation.

Symmetric snap-through buckling, although both theoretically achievable and practically advantageous, has remained rare in bistable systems, with most studies favoring asymmetric snapping due to its lower energy barrier. Previous observations of symmetric snapping have been limited to high loading rates. In this work, we present a universal strategy to achieve symmetric snapping under quasi-static conditions by designing magnetization (M)-interface patterns that effectively suppress asymmetric modes.

Evaluating the visceral adiposity inflammatory index for enhanced stroke risk assessment.

Stroke is a leading cause of global morbidity and mortality, with risk factors like visceral adiposity and inflammation playing significant roles. This study introduces the Visceral Adiposity Inflammatory Index (VAII), combining the Visceral Adiposity Index (VAI) and high-sensitivity C-reactive protein (CRP), to better predict stroke risk. Analyzing data from 8415 participants in the China Health and Retirement Longitudinal Study over 9 years, the study found that higher VAII levels were strongly associated with increased stroke incidence, with a hazard ratio of 1.

Global research trends in biomarkers, therapeutic targets, and drugs for amyotrophic lateral sclerosis: a bibliometric and visualization analysis.

Background: Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive degeneration of motor neurons, marked by complex pathological mechanisms and a lack of effective treatments. Despite substantial global research efforts, no comprehensive bibliometric analysis has systematically mapped the evolution of ALS biomarkers, therapeutic targets, and pharmacological advancements.

Methods: This study, based on 4,250 publications retrieved from the Web of Science Core Collection (2005-2025), employs bibliometric tools such as CiteSpace and VOSviewer to conduct the first multidimensional analysis of global trends in ALS biomarkers, therapeutic targets, and drug research.

Versatile spiky spindle-shaped copper-based nanocomposites: A SERS substrate for discrimination, quantification and inactivation of multiple bacteria.

Designing a multi-functional strategy that integrates rapid and sensitive detection of pathogenic bacteria and efficient antibacterial activity is of great significance. Here, we reported a smart triple-functional spindle shape copper-based nanocomposites, obtaining the remarkable surface-enhanced Raman scattering (SERS) enhancement, and its spikes at both ends with physical piercing ability cooperate with Ag and Cu releasing capability endowed it with robust antibacterial effectiveness. Originally, gold-core silver-shell nanoparticles (Au@Ag NPs) with excellent SERS performance were loaded onto the spindle shape copper-methylimidazole nanoparticles (Cu-MIM) under electrostatic action, obtaining Au@Ag/Cu-MIM nanocomposites.

A high-quality chromosome-level genome assembly of Antiaris toxicaria.

Objectives: Antiaris toxicaria is a tall tree belonging to the Moraceae family, known for its medicinal value. Its latex contains various cardiac glycosides, which hold significant research and potential application value. However, the lack of genomic resources for A.

Individual surgical management of trigeminal schwannomas guided by an extended classification: a consecutive series of 96 clinical cases at a single institution.

Trigeminal schwannoma (TS) is a rare intracranial neurinoma that affects the multicompartmental skull base. In recent decades, advancements in skull-base neurosurgical techniques and endoscopic surgery have significantly improved outcomes for TS patients. In this study, we present our experience with surgical resection of TS using a further classification system.

Orally Bioavailable and Site-Selective Covalent STING Inhibitor Derived from a Macrocyclic Marine Diterpenoid.

Pharmacological inhibition of the cGAS-STING-controlled innate immune pathway is an emerging therapeutic strategy for a myriad of inflammatory diseases. Here, we report as an orally bioavailable covalent STING inhibitor. Late-stage diversification of the briarane-type diterpenoid excavatolide B allowed the installation of solubility-enhancing functional groups while enhancing its activity as a covalent STING inhibitor against multiple human STING variants, including the S154 variant responsible for a genetic autoimmune disease.

An anoikis-related signature predicts prognosis and immunotherapy response in gastrointestinal cancers.

Background: Gastrointestinal (GI) cancers have high incidence rates and mortality rates. Anoikis is a special type of cell apoptosis, and anoikis resistance has been reported to be associated with tumor malignancy. We aimed to explore the roles of anoikis-related genes (ARGs) in the GI cancer prognosis.

Hydrostatic reduction of intussusception: the impact of high enema pressure on success rates.

Purpose: The aim of this study is to analyze the effect of increasing enema pressure on enema outcomes.

Methods: We conducted a retrospective study to compare the effect of increasing enema pressure on enema outcomes. The primary outcome was the success rate of reduction, while secondary outcomes included intestinal perforation and recurrence rate.

Acquired bla carried by a conjugative transposon or duplicated intrinsic bla mediates cefiderocol resistance in Elizabethkingia anophelis clinical isolates.

Objectives: Elizabethkingia spp. are resistant to multiple antibiotics. This study aimed to determine in vitro and in vivo activities of cefiderocol against Elizabethkingia spp.

A high-quality genome assembly reveals adaptations underlying glossy, wax-coated leaves in the heat-tolerant wild raspberry Rubus leucanthus.

With glossy, wax-coated leaves, Rubus leucanthus is one of the few heat-tolerant wild raspberry trees. To ascertain the underlying mechanism of heat tolerance, we generated a high-quality genome assembly with a genome size of 230.9 Mb and 24,918 protein-coding genes.

Discovery of a mu-opioid receptor modulator that in combination with morphinan antagonists induces analgesia.

Morphinan antagonists, which block opioid effects at mu-opioid receptors, have been studied for their analgesic potential. Previous studies have suggested that these antagonists elicit analgesia with fewer adverse effects in the presence of the mutant mu-opioid receptor (MOR; S196A). However, introducing a mutant receptor for medical applications represents significant challenges.

Transient Amplification of Broken Symmetry in Elastic Snap-Through.

A snap-through bifurcation occurs when a bistable structure loses one of its stable states and moves rapidly to the remaining state. For example, a buckled arch with symmetrically clamped ends can snap between an inverted and a natural state as the ends are released. A standard linear stability analysis suggests that the arch becomes unstable to asymmetric perturbations.

A high-quality chromosome-level genome assembly of Ficus hirta.

Ficus species (Moraceae) play pivotal roles in tropical and subtropical ecosystems. Thriving across diverse habitats, from rainforests to deserts, they harbor a multitude of mutualistic and antagonistic interactions with insects, nematodes, and pathogens. Despite their ecological significance, knowledge about the genomic background of Ficus remains limited.

The mitochondria-related gene risk mode revealed p66Shc as a prognostic mitochondria-related gene of glioblastoma.

Numerous studies have highlighted the pivotal role of mitochondria-related genes (MRGs) in the initiation and progression of glioblastoma (GBM). However, the specific contributions of MRGs coding proteins to GBM pathology remain incompletely elucidated. The identification of prognostic MRGs in GBM holds promise for the development of personalized targeted therapies and the enhancement of patient prognosis.