Dl-3-n-butylphthalein inhibits neuronal apoptosis and ferroptosis after cerebral ischemia-reperfusion injury in rats by regulating CXCR4.

Objective: To investigate the anti-apoptosis and anti-ferroptosis effects of dl-3-n-butylphthalide (dl-NBP) on cerebral ischemia-reperfusion injury (CIRI) in rats, and the potential involvement of cysteine-X-cysteine chemokine receptor 4 (CXCR4).

Methods: The differentially expressed genes between healthy people and stroke patients were screened by GEO database. A transient middle cerebral artery occlusion rat model was used to induce CIRI in vivo.

The association of triglyceride-glucose index and combined obesity indicators with chest pain and risk of cardiovascular disease in American population with pre-diabetes or diabetes.

Aim: To investigate the correlation of the triglyceride-glucose (TyG) index and its combined obesity indicators with chest pain and cardiovascular disease (CVD) in the pre-diabetes and diabetes population.

Methods: This cross-sectional investigation encompassed 6488 participants with diabetes and pre-diabetes who participated in the National Health and Nutrition Examination Survey (NHANES) between 2007 and 2016. The association of the TyG and combined obesity index with chest pain and CVD was investigated using weighted logistic regression models and restricted cubic spline (RCS) analysis.

Chemokine receptor 7 mediates miRNA-182 to regulate cerebral ischemia/reperfusion injury in rats.

Aims: Chemokine receptor 7 (CXCR7) exerts protective effects on the brain. MicroRNAs (miRNAs) are involved in cerebral ischemia/reperfusion (I/R) injury, but their involvement in CXCR7-mediated brain protection is unknown. In this study, we investigated the role of miRNAs in CXCR7-mediated brain protection.

How Information Repertoire Affects Vaccine Hesitancy: Processes of Information Verification and Cognitive Elaboration.

As information consumption plays a critical role in addressing vaccine hesitancy in the hybrid media environment, it becomes crucial to understand how individuals' use of a combination of channels and sources affects their vaccine hesitancy. Based on information repertoire approaches emphasizing the multiplicity of channels and sources, we investigated different patterns of information repertoire related to the COVID-19 pandemic and how these patterns affected vaccine hesitancy through different informational mechanisms. Our results based on a U.

Adsorption of water on the pristine and defective semiconducting 2D CrPmonolayers (: S, Se).

The effect of vacancy and water adsorption on the electronic structure of semiconducting 2D trichalcogenide material CrP(: S, Se) is studied using state-of-the-art density functional theory (DFT) approach. It is found that chalcogen vacancies play a minor role on the electronic structure of CrPin the vicinity of the Fermi level leading to the slightly reduced band gap for these materials, however, inducing strongly localised defect states which are placed in the energy gap formed by the valence band states. Our DFT calculations show that the interaction of water molecules with CrP, pristine and defective, can be described as physisorption and the adsorption energy for HO is insensitive to the difference between pristine and chalcogen-defective surface of trichalcogenide material.

Gas-solid interfacial charge transfer in volatile organic compound detection by CuCrOnanoparticles.

Nanostructured metal oxide semiconductors have received great attention used as the chemiresistive layer of gas sensor to detect the volatile organic compound recently. As indispensable complementary parts for dominative n-type semiconductors, the p-type metal oxides based gas sensors fail to be studied sufficiently, which hampers their practical applications. In this work, the p-type delafossite CuCrOnanoparticles were synthesized, characterized, and tested for gas sensing, followed by the first principles calculations to simulate the generation of chemiresistive signal.

Potential role of miR-155-5p in fat deposition and skeletal muscle development of chicken.

miR-155 has multiple functions in many physiological and pathological processes. However, little is known about the expression characteristics of avian miR-155. In the present study, partial pri-miR-155 sequences were cloned from AA+ broiler, Sanhuang broiler and Hy-Line Brown layer, respectively.

[Expression and function characterization of Gimap5 gene from different breeds of broilers].

GTPase immune-associated protein 5 (Gimap5), a key factor in maintaining T cell homeostasis, plays important roles in immune and inflammatory processes. However, its function and characteristics in poultry have not been reported. In this study, AA⁺ and Sanhuang broilers were used as models.

Microwave-assisted Synthesis of N,S-co-carbon Dots as Switch-on Fluorescent Sensor for Rapid and Sensitive Detection of Ascorbic Acid in Processed Fruit Juice.

To achieve a rapid, sensitive, and economical method for the detection of ascorbic acid (AA) in the presence of Fe, a nitrogen and sulfur co-doped carbon dots (N,S-co-CDs) based fluorescence sensing system was developed. In this work, N,S-co-CDs were successfully synthesized via a one-step microwave-assisted method within 2.5 min using ammonium citrate and L-cysteine as precursors.

Exploring the expression and preliminary function of chicken gene.

GTPase immune-associated protein 5 () plays a key role in maintaining T cell homeostasis, immunological tolerance and inflammatory processes. However, there are no reports on the chicken gene. In this study, the gene was first cloned from chicken and characterized its tissue expression characteristics in different developmental stages.

Arih2 gene influences immune response and tissue development in chicken.

Ariadne homolog 2 (ARIH2), as an E3 ubiquitin ligase, is one of the important factors involved in regulating biological functions, such as inflammation and skeletal muscle degeneration. In the present study, the full-length coding sequence of Arih2 gene was cloned from Hy-Line Brown chicken. The tissue transcriptional profiles of Arih2 gene at different developmental stages were detected using quantitative real-time PCR (qRT-PCR), and the Arih2 functional characteristics in immune response were analyzed.

Nutrients, phytochemicals and antioxidant activities of 26 kidney bean cultivars.

Detailed characterization in nutrients and phytochemicals with antioxidant activities of 26 kidney beans was performed. The kidney beans contained high levels of dietary fiber (29.32-46.

Metabolic profiling of Gynostemma pentaphyllum extract in rat serum, urine and faeces after oral administration.

Folk drug Gynostemma pentaphyllum (Thunb.) Makino contains many biologically active phytochemicals which have been demonstrated to be effective against chronic diseases. As in vivo anti-tumor experiments of G.

[Water extract from Codonopsis thalictrifolia wall affects the reproductive system of male infant rats].

Objective: To study the impact of the water extract from Codonopsis thalictrifolia Wall (CTW) on the reproductive

Methods: We divided 32 male SD infant rats into four groups of equal number to be treated intragastrical-system of male infant rats. ly with distilled water (control) and CTW at 10 g/kg (low dose) , 20 g/kg (medium dose), and 40 g/kg (high dose), respectively, twice a day for 2 weeks. Then we killed the rats, measured the levels of testosterone (T), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in the serum, obtained the testis weight, body weight, testis visceral coefficient and sperm concentration, and detected sperm viability, sperm motility and the level of cyclic adenosine monophosphate (cAMP) in the Leydig cells, followed by

Results: Compared with the control group, the low-dose, me-analysis of differences among different groups using the SPSS software.

Estradiol synthesis and release in cultured female rat bone marrow stem cells.

Bone marrow stem cells (BMSCs) have the capacity to differentiate into mature cell types of multiple tissues. Thus, they represent an alternative source for organ-specific cell replacement therapy in degenerative diseases. In this study, we demonstrated that female rat BMSCs could differentiate into steroidogenic cells with the capacity for de novo synthesis of Estradiol-17 β (E2) under high glucose culture conditions with or without retinoic acid (RA).

[STRA8 as a specific expression marker in postnatal male germ cells].

Stimulated by retinoic acid gene 8 (STRA8) is specifically expressed in mammalian germ cells before their transition from mitosis to meiosis. STRA8 expression is observed only in the postnatal testis. Single nucleotide polymorphisms but no mutations were identified in the coding or proximal promoter region of STRA8 in some gonadal dysgenesis patients.

[Application of fluorescence microscopic imaging technique with self-ordered ring to residues detection of antibiotics].

Fluorescence microscopic self-ordered ring (SOR) technique with microwave heating is proposed for minocycline based on the capillary flow of solvent on the surface of hydrophobic glass slide and applied to residues detection of the antibiotics in milks of Inner Mongolia. In the reaction medium of hexahydropyridine containing polyvinyl alcohol-124 (PVA-124), a SOR of minocycline with the outer diameter of 1.54 mm and the ring belt width of 22.

[Stra8: a specifically expressed gene in premeiotic germ cells' transition from mitosis to meiosis].

Stra8 (stimulated by retinoic acid gene 8) is a specific expression gene in mammalian germ cells' transition from mitosis to meiosis. Stra8 expresses in an anterior to posterior wave from embryonic days 12.5 to 16.

[Proto-oncogene c-src regulates the viability of rat spermatogonial stem cells in vitro through phosphorylated signal transducer and activator of transcription-3].

The present study aimed to investigate the effect of proto-oncogene c-src on the viability of rat spermatogonial stem cells from 9 day-old rat in vitro. MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense c-src oligodeoxynucleotides (ODNs) in vitro; RT-PCR was utilized to observe the expression of c-src mRNA and Western blot was used to observe the protein expressions of pp60c-src and phosphorylated signal transducer and activator of transcription-3 (p-STAT3). Compared with that in control group, the viability of spermatogonial stem cells decreased by 8.

[Expression of proto-oncogenes and its role in spermatogenic cells].

This article reviews the specific expression of many proto-oncogenes during male germ cell development. The normal expression of proto-oncogenes plays an important role in the regulation of spermatogonial mitosis, spermatocyte meiosis as well as spermiogenesis and sperm maturation.

[Effects of c-jun on hCG-induced testosterone secretion of rat Leydig cells in vitro].

Objective: To investigate the effects of c-jun on hCG-induced testosterone secretion in isolated rat Leydig cells by antisense oligodeoxynucleotides(ASODNs).

Methods: c-jun ASODNs were used to antagonise the effects of c-jun, hCG was used to induce the testosterone secretion of LC cultured in vitro and testosterone was measured by radioimmunoassay.

Results: The testosterone secretion of LC in vitro could be induced by hCG, which was a good model for the functional study of LC.

[Advances in the research of spermatogonial stem cell].

In recent years, people have paid more attention to the spermatogonial stem cells that have the capacity for self renewal and multilineage differentiation and produce daughter cells that can expand and differentiate into spermatozoa under the adjustment of self genes and external signal. This article reviews recent advances in studies of enrichment and original selection of the spermatogonial stem cells. This review also summarizes some control factors in proliferation and transplantation techniques.

[Leydig cell apoptosis and its regulation].

Apoptosis is necessary for the development and maturation of Leydig cells. However, increased apoptosis results the decline of testosterone production, which may increase germ cell apoptosis and the possibility of infertility. There are several aspects contributing to Leydig cell apoptosis such as ethane dimethanesulphonate (EDS), glucocorticoid, developmental stage and some hormones including FSH, LH/hCG and testosterone.