Autophagic-active nanosystem for senile bone regeneration by in-situ mitochondrial biogenesis and intercellular transfer.

Natural intercellular mitochondrial transfer has been recognized as a pivotal mechanism in the treatment of various diseases. Bone marrow mesenchymal stem cells (BMSCs), owing to their low bioenergetic demands and inherent homing capacity, are considered highly promising mitochondrial donor cells. However, this strategy is limited in senile osteoporosis (SOP) because large amounts of ROS produced by mitochondrial oxidative stress in senescent BMSCs (S-BMSCs) impairs their viability and function.

Peroxiredoxin 4 (Prx 4) plays important gene expression regulatory roles in antimicrobial innate immune by regulating hydrogen peroxide (HO) in the hepatopancreas of freshwater crayfish Procambarus clarkii.

This study focused on peroxiredoxin (Prx) as a core target to investigate its regulatory mechanism in the innate immunity of Procambarus clarkii (crayfish). We observed that a reduction in the expression of prx 4 via injection of double-stranded RNA (dsRNA) induced early upregulation of HO levels in the hepatopancreas of crayfish. When the crayfish were subsequently infected with Vibrio harveyi, the HO levels further increased, and the antioxidant enzyme system was activated.

Neuropeptide F (NPF) regulates the hepatopancreatic antimicrobial innate immune system by affecting the expression of antimicrobial peptides in Procambarus clarkii.

Neuropeptide F (NPF), a key component of the neuroendocrine-immune (NEI) system, is widely distributed in the central nervous system and peripheral immune cells and is involved in various physiological processes. In this study, the role of NPF in the NEI system of Procambarus clarkii was investigated, with a specific focus on its regulatory functions in innate immunity. We found that Pc-NPF is expressed in multiple tissues of P.

The impact of higher education investment on the urban-rural income gap: An analysis of mediating and threshold effects based on data from China's eight major comprehensive economic zones.

Background: Since the reform and opening up, China's urban and rural economic development has exhibited characteristics of imbalance, with the urban-rural income gap being the largest and most noticeable issue facing China's socio-economic landscape. Alleviating and effectively resolving the urban-rural income disparity is crucial for achieving overall common prosperity. Therefore, this study provides insights for strategically narrowing the urban-rural income gap from the perspective of higher education investment.

MSC-EV-transmitted HSPA8 alleviates cisplatin-induced ovotoxicity by regulating the MGARP/PRDX2 axis.

Cisplatin (Cis) is among the most widely employed antitumour agents, although its clinical application is limited by self-induced multiple-organ toxicity. Previous studies have demonstrated the essential role of mitochondrial injury in the pathogenesis of Cis-induced ovotoxicity. Notably, mesenchymal stem cell-extracellular vesicles (MSC-EVs), potential cell-free therapeutic agents, exhibit pronounced advantages for the treatment of ovarian dysfunction.

Multifaceted Catalytic Glucose Depletion and Reactive Oxygen Species-Scavenging Nanoenzyme Composite Hydrogel for Facilitating Diabetic Bone Regeneration.

Regeneration of diabetic bone defects remains a formidable challenge due to the chronic hyperglycemic state, which triggers the accumulation of advanced glycation end products (AGEs) and reactive oxygen species (ROS). To address this issue, we have engineered a bimetallic metal-organic framework-derived Mn@CoO@Pt nanoenzyme loaded with alendronate and Mg ions (termed MCPtA) to regulate the hyperglycemic microenvironment and recover the osteogenesis/osteoclast homeostasis. Notably, the Mn atom substitution in the CoO nanocrystalline structure could modulate the electronic structure and significantly improve the SOD/CAT catalytic activity for ROS scavenging.

Newton-Simpson-based predictor-corrector methods for milling chatter stability prediction.

Milling chatter, a form of self-excited vibration, can cause significant damage in machining and manufacturing processes. By selecting appropriate milling parameters, milling chatter can be effectively mitigated without sacrificing milling efficiency. Within the framework of the semi-discretization scheme, this paper introduces the Newton-Simpson-based predictor-corrector methods to compute milling stability lobe diagrams.

Construction of photo-induced zinc-doped carbon dots based on drug-resistant bactericides and their application for local treatment.

In this project, we propose a highly effective photosensitizer that breaks through drug-resistant bacterial infections with zinc-doped carbon dots. By passing through the membrane of drug-resistant bacteria, the photosensitizers produce ROS in bacteria under the action of blue light to directly kill bacteria, so as to realize the antibacterial local treatment of drug-resistant bacteria. The experiment firstly uses an efficient one-step hydrothermal method to prepare zinc-doped red-light CDs as photosensitizers, in which zinc metal was doped to improve the optical properties of the CDs.

Targeted therapy for the treatment of gliomas with multifunctional orange emissive carbon dots.

As a nano-material, carbon dots have been extensively studied and applied in many ways. Herein, iron-doped orange emissive carbon dots (ICDs) were easily synthesized using the hydrothermal method and coupled with Trf and glucose oxidase (GOD) simply by virtue of the abundant functional groups on their surface. The resulting carbon dots were named IGTCDs.

study of a novel, safe, rapid, and targeted red carbon dot probe for recognition of tumors with high expression of folate enzyme.

Carbon dots (CDS) have been proved to be a type of ideal biological imaging probe. They have the advantages of spontaneous fluorescence, anti-photobleaching, good biocompatibility and easy surface decoration, and are receiving special attention from researchers. The early imaging diagnosis of tumors has always been a practical means of clinical diagnosis.

An EHBP-1-SID-3-DYN-1 axis promotes membranous tubule fission during endocytic recycling.

The ACK family tyrosine kinase SID-3 is involved in the endocytic uptake of double-stranded RNA. Here we identified SID-3 as a previously unappreciated recycling regulator in the Caenorhabditis elegans intestine. The RAB-10 effector EHBP-1 is required for the endosomal localization of SID-3.

Two-photon fluorescence and fluorescence imaging of two styryl heterocyclic dyes combined with DNA.

Two new styryl heterocyclic two-photon (TP) materials, 4-[4-(N-methyl)styrene]-imidazo [4,5-f][1,10] phenanthroline-benzene iodated salt (probe-1) and 4,4-[4-(N-methyl)styrene]-benzene iodated salt (probe-2) were successfully synthesized and studied as potential fluorescent probes of DNA detection. The linear and nonlinear photophysical properties of two compounds in different solvents were investigated. The absorption, one- and two-photon fluorescent spectra of the free dye and dye-DNA complex were also examined to evaluate their photophysical properties.

[Study on the geographic information system databases regarding the control of schistosomiasis in Zhongxiang, Hubei province, China].

Objective: Using geographic information system (GIS) and the remote sensing techniques (RS), we developed a schistosomiasis database and geographic distribution map in Zhongxiang city,Hubei province in order to display and analyze the endemic situation longitudinally after the water conservancy project is completed.

Methods: Epidemiological data of schistosomiasis and the correlated climate and hydrology data for the last 30 years were collected and the relevant GIS databases were established under Artificial Neural Networks(ANN) and network training of Landsat TM images.

Results: GIS database of schistosomiasis in Zhongxiang city, Hubei province and its vicinity areas were developed including 1 maps regarding the epidemic situation of schistosomiasis.