An enzyme-free electrochemical biosensor for sensitive and specific detection of microRNA-21 based on target recycling amplification and non-linear hybridization chain reaction.

A novel electrochemical biosensor with high amplification efficiency was explored for serum microRNA-21 (miRNA-21) detection. This biosensor consisted of the target recycling amplification (TRA) and non-linear hybridization chain reaction (NHCR). When the target miRNA-21 was presented, hairpin probe 1 (HP) could be opened and hybridized with target miRNA-21, the formation of DNA/RNA complexes could hybridize with hairpin probe 2 (HP) and released target miRNA-21, resulting in the target recycling amplification.

Overexpression of the pullulan synthetase gene enhanced pullulan production and its molecular weight by a mutant of Aureobasidium melanogenum P16.

The pullulan synthetase gene (PUL1), involved in pullulan biosynthesis in Aureobasidium species, remains poorly understood. The open reading frame (ORF) of the PUL1 gene from the high pullulan-producing yeast Aureobasidium melanogenum P16 strain was cloned and characterized. The ORF of the PUL1 gene was determined to be 592 bp in length, encoding 178 amino acid residues.

Liamocins overproduction via the two-pH stage fermentation and anti-Aspergillus flavus activity of Massoia lactone.

Aureobasidium melanogenum was found to be grown the best at the constant pH 7.0 and to produce the highest amount of liamocins at the constant pH 3.0.

Gastric Mucosa-Associated Microbial Signatures of Early Gastric Cancer.

Alterations in the microbiome are associated with the development of gastric cancer. Our study aimed to identify dysbiotic features in early gastric cancer (EC). The gastric microbiome was assessed in EC ( = 30), advanced gastric cancer (AC) ( = 30), and chronic gastritis (CG) ( = 60).

Biglycan as a potential diagnostic and prognostic biomarker in multiple human cancers.

Biglycan (BGN), a key member of the small leucine-rich proteoglycan family, is an important component of the extracellular matrix. Clinical studies have demonstrated that upregulation of BGN is associated with poor prognosis in patients with various types of solid cancer. The present study analyzed the mRNA expression levels of BGN in various types of solid cancer when compared with that in normal tissues via the Oncomine database.

AKR1C1-3, notably AKR1C3, are distinct biomarkers for liver cancer diagnosis and prognosis: Database mining in malignancies.

Aldo-keto reductases, known as AKR1C1-AKR1C4 enzymes, are pivotal to NADPH-dependent reduction, and their expression is highly associated with the progression of malignant cancers. However, the expression and distinct prognostic value of the AKR1C family members in liver cancer are not well established. In the current study, the expression of AKR1C isoforms was studied using the Oncomine online databases.

Biosynthesis of some organic acids and lipids in industrially important microorganisms is promoted by pyruvate carboxylases.

Pyruvate carboxylase (Pyc) catalyzes formation of oxaloacetic acid from pyruvic acid by fixing one mole of CO2. Many evidences have confirmed that biosynthesis of some different kinds of organic acids and intracellular and extracellular lipids is driven by Pyc and over-expression of the PYC gene in the industrial microorganisms can promote production of the different kinds of organic acids and intracellular and extracellular lipids. Therefore, the Pyc from different sources is regarded as a key enzyme in microbial biotechnology and is an important target for metabolic engineering of the industrial microbial strains.

Association between gallstone and cardio-cerebrovascular disease: Systematic review and meta-analysis.

Increasing evidence connects gallstone disease (GD) to cardio-cerebrovascular disease (CVD). The aim of the present systematic review and meta-analysis was to determine whether and to what extent an association between GD and CVD existed. PubMed, EMBASE and the Cochrane Library were systemically searched up to March 3rd, 2018.

Combined enzymatic hydrolysis and selective fermentation for green production of alginate oligosaccharides from Laminaria japonica.

Alginate oligosaccharides (AOS) showed various biological activities. Traditional protocol for producing AOS was a multiple-step and high-pollution procedure. In this study, a rapid and efficient AOS producing method was developed directly from Laminaria japonica.

Genome sequencing of Aureobasidium pullulans P25 and overexpression of a glucose oxidase gene for hyper-production of Ca-gluconic acid.

Gluconic acid (GA) has many applications such as in the food and pharmaceutical industry. Aureobasidium pullulans P25 strain is able to produce high levels of Ca-GA. The genome length, GC content and the gene number of this yeast were found to be 30.

Cloning and Characterization of a Pyruvate Carboxylase Gene from Penicillium rubens and Overexpression of the Genein the Yeast Yarrowia lipolytica for Enhanced Citric Acid Production.

In this study, a pyruvate carboxylase gene (PYC1) from a marine fungus Penicillium rubens I607 was cloned and characterized. ORF of the gene (accession number: KM397349.1) had 3534 bp encoding 1177 amino acids with a molecular weight of 127.

Disruption of the acid protease gene in Saccharomycopsis fibuligera A11 enhances amylolytic activity and stability as well as trehalose accumulation.

The acid protease gene in Saccharomycopsis fibuligera A11 was disrupted by integrating the HPT (hygromycin B phosphotransferase) gene into ORF (Open Reading Frame) of the acid protease gene. The mutant 12 obtained could grow in the medium containing hygromycin. No clear zone formed by the mutant grown on the plate containing milk protein was observed whereas a big clear zone formed by the strain A11 was detected.

Mig1 is involved in mycelial formation and expression of the genes encoding extracellular enzymes in Saccharomycopsis fibuligera A11.

The MIG1 gene of Saccharomycopsis fibuligera A11 was cloned from its genomic DNA using the degenerated primers and inverse PCR. The MIG1 gene (1152bp, accession number: HM450676) encoded a 384-amino acid protein very similar to Mig1s from other fungi. Besides their highly conserved zinc fingers, the Mig1 proteins displayed short conserved motifs of possible significance in glucose repression.

Trehalose accumulation from cassava starch and release by a highly thermosensitive and permeable mutant of Saccharomycopsis fibuligera.

Highly thermosensitive and permeable mutants are the mutants from which intracellular contents can be released when they are incubated both in low osmolarity water and at non-permissive temperature (usually 37°C). After mutagenesis by using nitrosoguanidine, a highly thermosensitive and permeable mutant named A11-b was obtained from Saccharomycopsis fibuligera A11-12, a trehalose overproducer in which the acid protease gene has been disrupted. Of the total trehalose, 73.

Marine yeasts as biocontrol agents and producers of bio-products.

As some species of marine yeasts can colonize intestine of marine animals, they can be used as probiotics. It has been reported that beta-glucans from marine yeast cells can be utilized as immuno-stimulants in marine animals. Some siderophores or killer toxins produced by marine yeasts have ability to inhibit growth of pathogenic bacteria or kill pathogenic yeasts in marine animals.

[Eukaryotic expression of Leptospira interrogans lipL32/1-ompL1/1 fusion gene encoding genus-specific protein antigens and the immunoreactivity of expression products].

Objective: To construct the eukaryotic expression system of L.interrogans lipL32/1-ompL1/1 fusion gene and to identify the immunoreactivity of expression products.

Methods: PCR with linking primer was used to construct the fusion gene lipL32/1-ompL1/1.

[Effects of lactose inducing on expression of Helicobacter pylori rUreB and rHpaA, and Escherichia coli rLTKA63 and rLTB].

Objective: To determine the effects of lactose inducing on the expression of recombinant Helicobacter pylori rUreB and rhpaA, and Escherichia coli rLTB and rLTKA63.

Methods: BIO-RAD gel image analysis system was applied to detect the outputs of the recombinant proteins. SDS-PAGE was performed to measure the target protein expression of recombinant genes at various periods of growth, different lactose concentrations, various inducing temperatures and times.

Effects of lactose as an inducer on expression of Helicobacter pylori rUreB and rHpaA, and Escherichia coli rLTKA63 and rLTB.

Aim: To demonstrate the effect of lactose as an inducer on expression of the recombinant proteins encoded by Helicobacter pylori ureB and hpaA, and Escherichia coli LTB and LTKA63 genes and to determine the optimal expression parameters.

Methods: By using SDS-PAGE and BIO-RAD gel image analysis system, the outputs of the target recombinant proteins expressed by pET32a-ureB-E.coliBL21, pET32a-hpaA-E.

[Cloning, expression and identification of Escherichia coli LTB gene and Vibrio cholerae CTB gene].

Objective: To clone the LTB gene of E.coli and the CTB gene of V.cholerae, and to construct expression vectors of these genes.