Draft genome sequence and annotation of the enfumafungin producing fungus Hormonema carpetanum ATCC 74360.

Objectives: Plant endophytic fungusHormonema carpetanum is the only organism known to produce enfumafungin to date. Enfumafungin is a fernane-type triterpenoid glycoside compound with broad-spectrum antifungal activity, and this leading compound has been developed to antifungal agent ibrexafungerp approved by Food and Drug Administration (FDA). Here we report the first de novo assembled and annotated whole-genome sequence of H.

A corpus-based study of stance markers in academic thesis writing by L2 undergraduates.

The study of stance is an increasingly prominent area of research in academic and business writing. This study investigates the use of stance markers in two significant genres in higher education: L2 learners of business academic theses by and L1 British native learners. The study uses a corpus of maritime L2 learners and British Academic Writing English (BAWE) as a reference corpus for the corpus linguistic analysis.

Design, synthesis and biological evaluation of natural product echinocandin derivatives.

Fungal pathogen infections impose a great challenge for global public health, causing approximately 1.6 million deaths annually. Echinocandin-class drugs, with unique mechanism and favorable safety profile, have become the first-line anti-fungal therapeutic option for candidemia and invasive candidiasis.

Enhancement of tacrolimus production in Streptomyces tsukubaensis by genetic engineering and process optimization.

Tacrolimus, an important immunosuppressive drug widely used in clinical practice, is produced by Streptomyces tsukubaensis. In order to enhance the strain productivity, a high-yield mutant strain, 2108N-1-4, was obtained through natural isolation, screening and physical mutagenesis form S. tsukubaensis NRRL 18488.

An Investigation of Real-Time Galileo/GPS Integrated Precise Kinematic Time Transfer Based on Galileo HAS Service.

GNSS Precise Point Positioning (PPP) technology has been extensively applied to post-processing international comparisons between UTC/TAI times and real-time time transfer, predominantly in static configurations. However, with the swift advancement of intelligent and unmanned systems, there is an urgent need for research into kinematic time transfer. This paper introduces a kinematic model Galileo/GPS integrated PPP time transfer approach leveraging the Galileo High Accuracy Service (HAS).

Enhancement of spinosad production in Saccharopolyspora spinosa by overexpression of the complete 74-kb spinosyn gene cluster.

Background: Spinosad, a secondary metabolite produced by Saccharopolyspora spinosa, is a polyketide macrolide insecticide with low toxicity and environmental friendliness. Owing to the high level of DNA methylation and unclear regulatory mechanisms, gene engineering to increase spinosad production is challenging. Limited improvements in yield have been observed with heterologous expression or partial overexpression of the 74-kb spinosyn gene cluster (spn), and research on the overexpression of the complete spinosyn gene cluster is lacking.

Developing a robust genome editing tool based on an endogenous type I-B CRISPR-Cas system in Saccharopolyspora spinosa.

Saccharopolyspora spinosa is an industrial rare actinomycete capable of producing important environmental-friendly biopesticides, spinosyns. However, exploitation of S. spinosa has been limited due to its genetic inaccessibility and lack of effective genome engineering tools.

Effect of sodium-glucose Co-transporter 2 inhibitors on coronary microcirculation.

Coronary microvascular disease (CMVD) has emerged as a new target for the occurrence and development of heart failure treatment. Various indicators such as Index of Microvascular Resistance, Coronary Flow Reserve, Microvascular Resistance Reserve, Hyperemic Microvascular Resistance and Coronary Flow Velocity Reserve can be used to assess CMVD. Coronary microcirculation dysfunction is one of the important pathogenic mechanisms of heart failure.

Sediment dynamic responses of coastal salt marsh to wind waves and swells in a semi-open tidal flat.

Coastal salt marshes provide effective protection to the coastal environments they front against coastal erosion by reducing the incoming wave energy. Understanding sediment dynamic processes in coastal salt marshes environments is of crucial importance for coastal defense. The objective of this study is to assess the impact of Spartina alterniflora (S.

Enhancement of doxorubicin production in Streptomyces peucetius by genetic engineering and process optimization.

Doxorubicin is an important class of anthracycline antitumor antibiotics produced by Streptomyces peucetius. The doxorubicin fermentation yield of the wild-type strain was very low, so it could not be produced directly by fermentation at an industrial scale due to the high cost. In the present study, S.

Enhancement of fungichromin production of Streptomyces sp. WP-1 by genetic engineering.

Fungichromin is a polyene macrolide antibiotic with potent killing activity against a broad range of agricultural pathogens and filamentous fungi and a wide range of potential applications. The production of fungichromin is still hampered by poor fermentation yield and high cost. In this study, the whole genome sequencing of fungichromin-producing Streptomyces sp.

Construction of an efficient cell factory for lysergic acid production.

Lysergic acid (LA) is the key precursor of ergot alkaloids, and its derivatives have been used extensively for the treatment of neurological disorders. However, the poor fermentation efficiency limited its industrial application. At the same time, the hardship of genetic manipulation has hindered the metabolic engineering of strains to improve the LA titer further.

Engineering Methyltransferase and Sulfoxide Synthase for High-Yield Production of Ergothioneine.

Ergothioneine (ERG) is an unusual sulfur-containing amino acid with antioxidant activity that can be synthesized by certain bacteria and fungi. Microbial fermentation is a promising method for ERG production. In this study, the bifunctional enzyme methyltransferase-sulfoxide synthase NcEgt1 from was truncated to obtain sulfoxide synthase NcEgt1, which showed a higher expression level in BL21(DE3).

Enhancement of acarbose production by genetic engineering and fed-batch fermentation strategy in Actinoplanes sp. SIPI12-34.

Background: Acarbose, as an alpha-glucosidase inhibitor, is widely used clinically to treat type II diabetes. In its industrial production, Actinoplanes sp. SE50/110 is used as the production strain.

Association of Impaired Fasting Blood Glucose With Triple Coronary Artery Stenosis and Myocardial Infarction Among Patients With Coronary Artery Stenosis.

The association between impaired fasting glucose level (IFG) and coronary heart disease (CAD) remain controversial. In the present study, we sought to ascertain a relationship of IFG with the number of diseased coronary artery and occurrence of myocardial infarction, among CAD cases. We studied 1,451 consecutive no-diabetic patients who underwent coronary angiography at the First Affiliated Hospital of Shantou University Medical College in Southern China.

CRISPR/Cas9-Based Genome Editing in the Filamentous Fungus and Its Application in Manipulating .

is an important industrial fungus that produces the pneumocandin B, which is used for the synthesis of antifungal drug caspofungin. However, because of the limitations and complications of traditional genetic tools, strain engineering has been hindered. In this study, we established an efficient CRISPR/Cas9-based gene editing tool in SIPI1208.

Amentotaxins C-V, Structurally Diverse Diterpenoids from the Leaves and Twigs of the Vulnerable Conifer and Their Cytotoxic Effects.

A phytochemical investigation of the MeOH extract of the leaves and twigs of , a relict vulnerable coniferous species endemic to China, led to the isolation and characterization of 35 diterpenoids/norditerpenoids. Twenty of these are new, including 11 -kaurane-type (amentotaxins C-M, -, respectively), three icetexane-type [= 9(10→20)-abietane-type (amentotaxins N-P, -, respectively)], four -labdane-type (amentotaxins Q-T, -, respectively), and two isopimarane-type [amentotaxins U () and V ()] compounds. Their structures were elucidated on the basis of spectroscopic data, single-crystal X-ray diffraction, the modified Mosher's method, and electronic circular dichroism data analyses.

UPLC-MS identification and anticomplement activity of the metabolites of Sophora tonkinensis flavonoids treated with human intestinal bacteria.

Anticomplement activity played an important role in anti-inflammatory effects of traditional Chinese herbs. The total flavonoids of Sophora tonkinensis (TFST) were inactive on the complement system but showed obvious anticomplement activity after incubated with human intestinal bacteria in vitro. In order to discover the metabolic activation of TFST by intestinal flora, the constituents of TFST and its metabolites were identified by UPLC-ESI-LTQ/MS.

Enhancement of FK520 production in Streptomyces hygroscopicus by combining traditional mutagenesis with metabolic engineering.

FK520 (ascomycin), a 23-membered macrolide with immunosuppressive activity, is produced by Streptomyces hygroscopicus. The problem of low yield and high impurities (mainly FK523) limits the industrialized production of FK520. In this study, the FK520 yield was significantly improved by strain mutagenesis and genetic engineering.

Enhancement of ascomycin production via a combination of atmospheric and room temperature plasma mutagenesis in Streptomyces hygroscopicus and medium optimization.

Ascomycin, a key intermediate for chemical synthesis of immunosuppressive drug pimecrolimus, is produced by Streptomyces hygroscopicus var. ascomyceticus. In order to improve the strain production, the original S.

aMSGE: advanced multiplex site-specific genome engineering with orthogonal modular recombinases in actinomycetes.

Chromosomal integration of genes and pathways is of particular importance for large-scale and long-term fermentation in industrial biotechnology. However, stable, multi-copy integration of long DNA segments (e.g.