KU4 inhibits adipocyte senescence in aged mice through necdin regulation of p53 activity.

Previously, we reported that KU4 (LKU4) ameliorates diet-induced metabolic disorders by regulating adipose tissue (AT) physiology. Since metabolic disorders and age-related pathological conditions mutually exacerbate each other, this study hypothesizes that LKU4 may protect against adipose senescence during aging. Thus, this study demonstrates that LKU4 administration suppresses age-related metabolic dysfunction and aging phenotypes in AT of 24-month-old mice.

Alleviating peripartum oxidative stress and enhancing maternal behavior in loose-housed hyperprolific sows improves farrowing process and piglet performance: effects of ferulic acid, probiotic mixture, or jute sacks.

Sows with large litters often experience challenges, such as prolonged farrowing, higher neonatal mortality, and compromised maternal behavior, which have recently been linked to excessive oxidative stress. This study evaluated the effects of ferulic acid (FA) and probiotic mixture (PM) on oxidative stress reduction during the prepartum and lactation periods in hyperprolific (HP) sows and the jute sack (JS) provision on maternal instinct enhancement to assess their impact on the farrowing process, maternal behavior, immune status, and piglet performance. A total of 42 sows (Landrace × Yorkshire, n = 30, parity = 2.

Whole genome sequence of subsp. JNU311 isolated from infant feces.

The genome of subsp. JNU311, isolated from the feces of a breastfed infant, was sequenced. The circular genome comprises 2,610,619 bp with 59.

Complete genome sequencing of NS2301G1 isolated from kimchi.

Sequencing of the NS2301G1 strain isolated from kimchi revealed a 2,968,519 bp circular chromosome with a 46.74% GC content; in addition, genes linked to probiotic properties, stress tolerance, and carbohydrate metabolism were identified, highlighting the strain's potential application in the biotechnology and food industries.

Environmental Stress on JNU532's Cellular Characteristics and Fatty Acid Profile.

This study aimed to investigate the effects of various growth conditions and nutritional stress on the rate of cell development, auto and co-aggregation abilities, cell surface hydrophobicity, adherence to HT-29 cells, and changes in cellular fatty acid composition of JNU532 (JNU532). The strain JNU532 was exposed to different stressors, including temperature variations (37°C and 42°C), salt (3% NaCl), acid (pH 4), and alkali (pH 8). Following exposure to temperature stress at 42°C, the strain demonstrated the highest levels of auto- and co-aggregation, but the lowest levels of hydrophobicity and adhesion abilities.

A method for explaining individual predictions in neural networks.

Background: Recently, the explainability of the prediction results of machine learning models has attracted attention. Most high-performance prediction models are black boxes that cannot be explained. Artificial neural networks are also considered black box models.

Ultra-tiny-scale technology for engineering human ear therapeutics.

Ultra-tiny-scale technology representing engineered micro- and nano-scale materials has gained considerable attention for a wide range of applications, including hearing restoration. The advent of hearing loss and its recovery has been the topic of intense discussion since many decades. Although conventional treatments partially support hearing recovery, they present certain limitations such as subsequent immune response and donor site morbidity leading to even worsened sensory disturbances.

An effective heuristic for developing hybrid feature selection in high dimensional and low sample size datasets.

Background: High-dimensional datasets with low sample sizes (HDLSS) are pivotal in the fields of biology and bioinformatics. One of core objective of HDLSS is to select most informative features and discarding redundant or irrelevant features. This is particularly crucial in bioinformatics, where accurate feature (gene) selection can lead to breakthroughs in drug development and provide insights into disease diagnostics.

Robust metabolic syndrome risk score based on triangular areal similarity.

One of the limitations of currently-used metabolic syndrome (MetS) risk calculations is that they often depend on sample characteristics. To address this, we introduced a novel sample-independent risk quantification method called 'triangular areal similarity' (TAS) that employs three-axis radar charts constructed from five MetS factors in order to assess the similarity between standard diagnostic thresholds and individual patient measurements. The method was evaluated using large datasets of Korean ( = 72,332) and American ( = 11,286) demographics further segmented by sex, age, and race.

Complete genome and two plasmids sequences of L55 for probiotic potentials.

In this study, we report the complete genome sequence of L55, a probiotic strain of lactic acid bacteria isolated from kimchi. The genome consists of one circular chromosome (2,077,416 base pair [bp]) with a guanine cytosine (GC) content of 44.5%, and two circular plasmid sequences (54,267 and 19,592 bp, respectively).

Persisters Formation and Resuscitation.

is a commonly used probiotic, and many researchers have focused on its stress response to improve its functionality and survival. However, studies on persister cells, dormant cells that aid bacteria in surviving general stress, have focused on pathogenic bacteria that cause infection, not . Thus, understanding persister cells will provide essential clues for understanding how survives and maintains its function under various environmental conditions.

Lactobacillus amylovorus KU4 induces adipose browning in obese mice by regulating PP4C.

We previously reported that Lactobacillus amylovorus KU4 (LKU4) promotes adipocyte browning in mice fed a high-fat diet (HFD mice) in part by remodeling the PPARγ transcription complex. However, the mechanism through which LKU4 enables PPARγ to drive adipocyte browning remains elusive. Here, we report that LKU4 inhibits the expression of PP4C in inguinal white adipose tissue of HFD mice and in insulin-resistant 3T3-L1 adipocytes, which promotes SIRT1-dependent PPARγ deacetylation by activating AMPK, leading to the browning of adipocytes.

Inhibitory Effects of BYB3 Cell-Free Extract on Human Melanoma B16F10 Cells and Tumorigenic Mice.

BYB3 (BYB3) is a species of lactic acid bacteria, formerly named , which is isolated from kimchi. In this study, the effect of BYB3, GG, and GP1B strain extracts at various concentrations was examined on B16F10, a mouse melanoma cell line. Cell viability was examined via MTT assay, and the results indicated that compared to the other two probiotics, BYB3 significantly decreased the total percentages of viable cells.

The PKA-SREBP1c Pathway Plays a Key Role in the Protective Effects of Lactobacillus johnsonii JNU3402 Against Diet-Induced Fatty Liver in Mice.

Scope: The present study aims to assess the protective effect of Lactobacillus johnsonii JNU3402 (LJ3402) against diet-induced non-alcoholic fatty liver disease (NAFLD) and determine the mechanism underlying its beneficial effect on the liver in mice.

Methods And Results: Seven-week-old male mice are fed a high-fat diet (HFD) with or without oral supplementation of LJ3402 for 14 weeks. In mice fed an HFD, LJ3402 administration alleviates liver steatosis, diet-induced obesity, and insulin resistance with a decreased hepatic expression of sterol-regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC), and an increased phosphorylation of SREBP-1c.

Probiotics that Ameliorate Cognitive Impairment through Anti-Inflammation and Anti-Oxidation in Mice.

The gut-brain axis encompasses a bidirectional communication pathway between the gastrointestinal microbiota and the central nervous system. There is some evidence to suggest that probiotics may have a positive effect on cognitive function, but more research is needed before any definitive conclusions can be drawn. Inflammation-induced by lipopolysaccharide (LPS) may affect cognitive function.

Machine learning-based predictive model for prevention of metabolic syndrome.

Metabolic syndrome (MetS) is a chronic disease caused by obesity, high blood pressure, high blood sugar, and dyslipidemia and may lead to cardiovascular disease or type 2 diabetes. Therefore, the detection and prevention of MetS at an early stage are imperative. Individuals can detect MetS early and manage it effectively if they can easily monitor their health status in their daily lives.

Complete genome sequence of JNU532 as a probiotic candidate for the functional food and feed supplements.

Lactic acid bacteria (LAB) have been reported to possess various beneficial properties and are commonly used as probiotics. LAB play a crucial role in milk fermentation, industrial lactic acid fermentation, and health and medicine. isolated from fermented dairy and food products is considered as 'Generally Recognized as Safe' by FDA.

Metabolites of BYB3 and Indole Activate Aryl Hydrocarbon Receptor to Attenuate Lipopolysaccharide-Induced Intestinal Barrier Dysfunction.

This study aimed to investigate the effects of the metabolites of BYB3 and indole-activated aryl hydrocarbon receptor (AhR) to increase the tight junction (TJ) proteins in an model of intestinal inflammation. In a Western blot assay, the metabolites of BYB3 reduced the TJ demage in lipoploysaccharide (LPS) stimulated-Caco-2 cells. This reduction was a result of upregulating the expression of TJ-associated proteins and suppressing the nuclear factor-κB signaling.

Culturomic-, metagenomic-, and transcriptomic-based characterization of commensal lactic acid bacteria isolated from domestic dogs using Caenorhabditis elegans as a model for aging.

In tandem with the fast expansion of the pet-economy industry, the present aging research has been noticing the function of probiotics in extending the healthy lifetime of domestic animals. In this study, we aimed to understand the bacterial compositions of canine feces and isolating lactic acid bacteria (LAB) as commensal LAB as novel potential probiotics for the use of antiaging using Caenorhabditis elegans surrogate animal model. Under an anaerobic, culturomic, and metagenomic analysis, a total of 305 commensal LAB were isolated from diverse domestic dogs, and four strains, Lactobacillus amylolyticus, L.

Complete genome sequences of JNU 534, a potential food and feed preservative.

A new bacteriocin-producing lactic acid bacteria isolated from kimchi was identified as JNU 534, presenting preservative properties for foods of animal origin. In this study, we present the complete genome sequence of the bacterial strain JNU 534. The final complete genome assembly consists of one circular chromosome (2,443,687 bp [base pair]) with an overall GC (guanine-cytosine) content of 35.

Roles of Milk Fat Globule Membrane on Fat Digestion and Infant Nutrition.

Milk fats are present as globules emulsified in the aqueous phase of milk and stabilized by a delicate membrane architecture called milk fat globule membrane (MFGM). The unique structure and composition of the MFGM play an important role in fat digestion and the metabolic programming of neonates. The objective of this review is to compare the structure, composition, and physicochemical characteristics of fat globules in human milk, bovine milk, and infant formula.

Development of the Integrated Glaucoma Risk Index.

Various machine-learning schemes have been proposed to diagnose glaucoma. They can classify subjects into 'normal' or 'glaucoma'-positive but cannot determine the severity of the latter. To complement this, researchers have proposed statistical indices for glaucoma risk.

BYB3 Isolated from Kimchi Alleviates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice by Inhibiting IL-6 and TNF-R1 Production.

Recent studies have shown that probiotics have health-promoting effects, particularly intestinal immune modulation. In this study, we focused on the immunomodulatory properties of BYB3, formerly called , isolated from kimchi. In a mouse model of 14-day dextran sulfate sodium (DSS)-induced colitis, treatment with BYB3 significantly decreased the disease activity index, colon length, and weight loss.

Genome analysis of JN2019 applied to tumeric fermentation for animal feed.

JN2019, formerly named JN2019, was isolated from kimchi. Its genome was completely sequenced using the PacBio RSII sequencing system to explore beneficial phenotypes. In a previous study, JN2019 was used to ferment the by-product of tumeric for use in livestock feed.