Exploration of ESIPT in 4,6-O-Ethylidene-N-(2-hydroxybenzylidene)-β-D-glucopyranosylamine and Its Application in Selective Turn-on Response Toward Lu(III) ion.

4,6-O-Ethylidene-N-(2-hydroxybenzylidene)-β-D-glucopyranosylamine (PL1) exhibits excited state intramolecular proton transfer (ESIPT) mechanism, which has been established by experimental and theoretical calculations. Further, PL1 selectively interacts with Lu ion yielding a turn-on response in methanol, where the emission intensity of the former enhances (~15 fold) after metal ion interaction. The complexation has been investigated using various analytical tools like UV-visible absorption/emission, nuclear magnetic resonance spectroscopy and lifetime studies.

A cross-sectional survey of ocular problems after COVID-19 infection among healthcare workers at a teaching hospital.

Purpose: The COVID-19 pandemic was caused by severe acute respiratory syndrome coronavirus-2 (SARS-COV2). The impact of the pandemic is becoming more apparent now on various tissues and body organs. Limited knowledge is present about the characteristics, duration, and long-term sequelae of ocular problems post-COVID-19 infection.

Comparative analysis of parametric B-spline and Hermite cubic spline based methods for accurate ECG signal modeling.

Analyzing Electrocardiogram (ECG) signals is imperative for diagnosing cardiovascular diseases. However, evaluating ECG analysis techniques faces challenges due to noise and artifacts in actual signals. Machine learning for automatic diagnosis encounters data acquisition hurdles due to medical data privacy constraints.

Phage for drug delivery vehicles.

Viruses being the natural carriers of gene have been widely used as drug delivery systems. However, the commonly used eukaryotic viruses such as adenoviruses, retroviruses, and lentiviruses, besides efficiently targeting the cells, can also stimulate immunological response or disrupt tumour suppressor genes leading to cancer. Consequently, there has been an increase interest in the scientific fraternity towards exploring other alternatives, which are safer and equally efficient for drug delivery.

Chain length effect of spiro-ring -alkylation on photophysical signalling parameters in Fe(III) selective rhodamine probes.

Manifestation of photophysical signalling parameters in rhodamine derivatives exhibiting complexation induced spiro-ring opening is crucial for the realization of selective metal ion detection at trace levels. Substitution of various functional groups, such as alkylation to the core architecture, modulates the physico-chemical properties of such molecular probes. Despite a few studies, relationships between the extent of photophysical signal modulations and the chain lengths of -alkyl substituents are still elusive.

Current status and applications of genus Geobacillus in the production of industrially important products-a review.

The genus Geobacillus is one of the most important genera which mainly comprises gram-positive thermophilic bacterial strains including obligate aerobes, denitrifiers and facultative anaerobes having capability of endospore formation as well. The genus Geobacillus is widely distributed in nature and mostly abundant in extreme locations such as cool soils, hot springs, hydrothermal vents, marine trenches, hay composts and dairy plants. Due to plasticity towards environmental adaptation, the Geobacillus sp.

Clinical Profiles of Neonates Born to COVID-19 Positive Mothers in a Tertiary Care Centre: A Descriptive Cross-sectional Study.

Introduction: Nepal is one of the countries which has been hit hard by the COVID-19 pandemic and has resulted in high morbidity and mortality across all age groups including neonates. There has been a paucity of studies regarding maternal to neonatal COVID-19 transmission and the published studies also have a poor sample size to reach any definite conclusion. Hence this study was carried out to see the clinical profiles of neonates born to COVID-19 mothers.

A rapid method for solubilization of chimeric human interferon regulatory factor-1 (IRF-1) protein in Escherichia coli.

Interferon regulatory factor-1 (IRF-1) is a vertebrate transcription factor that plays significant roles in cell cycle regulation, anti-viral response, tumor suppression and immune response. High-level expression of recombinant IRF-1 at 37 °C leads to the formation of insoluble aggregates (insoluble fraction) in Escherichia coli (E. coli), which usually devoid of biological activity.

Reduced-sodium cheeses: Implications of reducing sodium chloride on cheese quality and safety.

Sodium chloride (NaCl) universally well-known as table salt is an ancient food additive, which is broadly used to increase the storage stability and the palatability of foods. Though, in recent decades, use of table salt in foods is a major concern among the health agencies of the world owing to ill effects of sodium (Na) that are mostly linked to hypertension and cardiovascular diseases. As a result, food scientists are working to decrease the sodium content in food either by decreasing the rate of NaCl addition or by partial or full replacement of NaCl with other suitable salts like potassium chloride (KCl), calcium chloride (CaCl ), or magnesium chloride (MgCl ).

Role of TRP Channels in Shaping the Gut Microbiome.

Transient receptor potential (TRP) channel family proteins are sensors for pain, which sense a variety of thermal and noxious chemicals. Sensory neurons innervating the gut abundantly express TRPA1 and TRPV1 channels and are in close proximity of gut microbes. Emerging evidence indicates a bi-directional gut-brain cross-talk in several entero-neuronal pathologies; however, the direct evidence of TRP channels interacting with gut microbial populations is lacking.

Effect of n-alkyl substitution on Cu(ii)-selective chemosensing of rhodamine B derivatives.

Rhodamine B hydrazide-based molecular probes (1-10) were synthesized by derivatization with n-alkyl chains of different lengths at the hydrazide amino end. These probes exhibited selective absorption (A∼557) and fluorescence (I∼580) 'off-on' signal transduction along with a colourless → magenta colour transition in the presence of Cu(ii) ions among all the competitive metal ions investigated. The effective coordination of these probes to Cu(ii) ions under the investigated environment forming [Cu·L]2+ (L = 1-5) and [Cu·L2]2+ (L = 6-10) complexes led to their spiro-ring opening, which in turn was expressed through signatory spectral peaks of ring-opened rhodamine.

Recombinant human interferon regulatory factor-1 (IRF-1) protein expression and solubilisation study in Escherichia coli.

Interferon regulatory factor-1 (IRF-1) is a tumor suppressor gene, which encodes a mammalian transcription factor that serves various vital functions in a cell, such as cell cycle regulation, immunomodulation, and antiviral response. We report full-length human IRF-1 cDNA cloning and expression in E. coli/BL21 cells with complete solubilisation of recombinant protein.

Experimental Protocol for Cecal Ligation and Puncture Model of Polymicrobial Sepsis and Assessment of Vascular Functions in Mice.

Sepsis is the systemic inflammatory response syndrome that occurs during infection and is exacerbated by the inappropriate immune response encountered by the affected individual. Despite extensive research, sepsis in humans is one of the biggest challenges for clinicians. The high mortality rate in sepsis is primarily due to hypoperfusion-induced multiorgan dysfunctions , resulting from a marked decrease in peripheral resistance.

Brain natriuretic peptide (BNP) expressing sensory neurons are not involved in acute, inflammatory or neuropathic pain.

Background: We recently demonstrated that brain natriuretic peptide is expressed in the dorsal root ganglia, and that brain natriuretic peptide is required for normal detection of pruritogens. We further showed that the receptor for brain natriuretic peptide, natriuretic peptide receptor A, is present in the spinal cord, and elimination of these neurons profoundly attenuates scratching to itch-inducing compounds. However, the potential modulatory roles of brain natriuretic peptide in nociception, inflammation, and neuropathic mechanisms underlying the sensation of pain have not been investigated in detail.

Antibiotic sensitivity pattern of indigenous lactobacilli isolated from curd and human milk samples.

The gut microbiota plays a vital role in host well-being and lactic acid bacteria (LAB) have gained an overwhelming attention as health promoter. This perception has evolved from traditional dairy products to a money-spinning market of probiotics. The safety of probiotics is coupled to their intended use and LAB may act as pool of antimicrobial resistance genes that could be transferred to pathogens, either in food matrix or in gastrointestinal tract, which could be detrimental to host.

Antibacterial effects of Lactobacillus isolates of curd and human milk origin against food-borne and human pathogens.

This study was undertaken to assess the antibacterial efficacy of lactobacilli isolated from curd and human milk samples. Identities of thirty-one different lactobacilli (20 from curd and 11 from human milk) were confirmed by genus-specific PCR and 16S rRNA-based sequencing. These strains belonged to five species, Lactobacillus casei, L.

Dietary Polysaccharides in the Amelioration of Gut Microbiome Dysbiosis and Metabolic Diseases.

The prevalence of metabolic diseases including obesity, diabetes, cardiovascular diseases, hypertension and cancer has evolved into a global epidemic over the last century. The rate of these disorders is continuously rising due to the lack of effective preventative and therapeutic strategies. This warrants for the development of novel strategies that could help in the prevention, treatment and/ or better management of such disorders.

BAY 41-2272 Treatment Improves Acetylcholine-Induced Aortic Relaxation in L-NAME Hypertensive Rats.

Hypertension, an emerging problem of recent era, and many pathophysiological factors are participating to produce the disease. Nitric oxide (NO) is an important constituent to ameliorate hypertensive condition. Inhibition of endogenous NO synthase by L-N-Nitroarginine methyl ester (L-NAME) was responsible for generating hypertension in rats.

Subchronic Arsenic Exposure Through Drinking Water Alters Lipid Profile and Electrolyte Status in Rats.

Arsenic is a groundwater pollutant and can cause various cardiovascular disorders in the exposed population. The aim of the present study was to assess whether subchronic arsenic exposure through drinking water can induce vascular dysfunction associated with alteration in plasma electrolytes and lipid profile. Rats were exposed to arsenic as 25, 50, and 100 ppm of sodium arsenite through drinking water for 90 consecutive days.

NO synthase inhibition attenuates EDHF-mediated relaxation induced by TRPV4 channel agonist GSK1016790A in the rat pulmonary artery: Role of TxA2.

Background: The aim of the present study was to observe the concomitant activation of nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF) pathways by TRPV4 channel agonist GSK1016790A in the rat pulmonary artery and explore the mechanism by which NO synthase inhibition attenuates EDHF-mediated relaxation in endothelium-intact rat pulmonary artery.

Methods: Tension experiments were conducted on the pulmonary artery from male Wistar rats.

Results: TRPV4 channel agonist GSK1016790A (GSK) caused concentration-dependent relaxation (Emax 86.