Exploring the mechanism of flavonoid amination and its potential biological implications from an and perspective.

The discovery of flavonoid amination metabolism has raised research interest in this new biotransformation mechanism. studies have revealed that flavonoids with pyrogallol structures readily react with N-nucleophilic ammonia, where amination occurs at intermediate -OH position, such as the B ring C4'-OH of epigallocatechin gallate and myricetin, A ring C6-OH of baicalein. Flavonoids are also covalently bound with amino acid residue by Schiff base, Michael addition or Strecker degradation, which are further rearranged to generate -NH substituted products.

MDS-associated mutations promote aberrant fate choice of hematopoietic stem cell via mis-splicing of mediator kinase module component CDK8.

Mutations in the RNA splicing factor are among the most common in MDS and are strongly associated with MDS with ring sideroblasts (MDS-RS). While aberrant splicing of terminal erythroid regulators has been implicated in MDS pathogenesis, the impact of mutations on early hematopoietic progenitor function remains unclear. Here, we identify CDK8, a key kinase of the mediator complex involved in transcriptional regulation, as a recurrent mis-spliced target in -mutant MDS.

A national soil organic carbon density dataset (2010-2024) in China.

Soil organic carbon (SOC) is a vital component of terrestrial ecosystems and a key indicator of soil quality, agricultural potential, and climate regulation. Despite growing interest in SOC distribution across China, high-resolution and standardized datasets remain scarce. Here, we present a national soil organic carbon density (SOCD) dataset containing 23,103 samples from 7,852 soil profiles collected between 2010 and 2024.

KAT6A chimeras form a self-reinforcing epigenetic module with NURF and MLL/COMPASS to sustain AML.

Background: KAT6A-CBP (K/C) and KAT6A-P300 (K/P) fusions are recurrent genetic alterations in acute myeloid leukemia (AML) associated with poor prognosis. Despite their strong oncogenic potential, the mechanisms underlying their genomic targeting and leukemogenic function remain unclear. A major challenge has been their large size, which has impeded preclinical model development and mechanistic studies.

First Report of Causing Root Rot of Thunb. in China.

Lonicera japonica Thunb., belonging to Caprifoliaceae, is a widely cultivated traditional Chinese medicinal plant with high economic value. In May of 2024, symptoms of a root rot disease were observed on L.

A clinical study on ozone autohemotherapy for the treatment of acute ischemic stroke.

Background: Ozonated autohemotherapy is a therapeutic method that utilizes the contact of medical ozone with blood to achieve the effect of ozone oxidative preconditioning, and then re-infuses it back into the human body. It has the functions of alleviating oxidative stress damage, reducing neuroexcitation toxicity, and mitigating cellular edema and inflammatory responses. Cerebral infarction has a significant social hazard, and there are multiple treatment methods available.

Effectiveness of the sleep apnea-specific hypoxic burden and sleep breathing impairment index in assessing cognitive impairment in children with obstructive sleep apnea.

Purpose: This study investigated cognitive impairment in children with obstructive sleep apnea (OSA) by evaluating the utility of sleep apnea-specific hypoxic burden (SASHB) and sleep breathing impairment index (SBII) compared to the obstructive apnea-hypopnea index (OAHI).

Methods: A retrospective analysis included 141 children with suspected OSA from Xi'an Jiaotong University Second Affiliated Hospital (October 2021-October 2024), categorized into OSA ( = 104) and non-OSA ( = 37) groups based on OAHI. Demographic, polysomnography (PSG), and event-related potential (ERP) data were collected.

Metabolic potentials of the gut microbes in Antarctic krill ().

Antarctic krill () plays crucial roles in the Southern Ocean biological system, yet the ecological functions of their gut microbiota remain poorly understood. The study investigated the composition and diversity of the gut microbiota in using 16S rRNA gene sequencing, revealing a highly diverse and tissue-specific microbiota. Further investigation through direct metagenomics and culture-enriched metagenomics identified 12 metagenome-assembled genomes (MAGs), which might represent novel species based on their phylogenetic positions, average nucleotide identity (ANI), average amino acid identity (AAI), and relative evolutionary divergence (RED) values.

Activation of a nongenetic AHR-ELMSAN1 axis optimizes BET-targeting therapy and suppresses leukemia stem cells in preclinical models.

Developing strategies to enhance the response to bromodomain and extraterminal domain (BET) inhibitors and effectively eradicate cancer stem cells would represent a major cancer treatment advance against leukemia. Through a functional CRISPR screen, we identified the aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor, as a critical regulator of MYC expression and BET inhibitor sensitivity in human acute myeloid leukemia (AML). Constitutive or pharmacological activation of AHR repressed MYC and synergized with BET inhibitors to inhibit MYC transcription and suppress leukemia growth across diverse AML models.

Association of memory function with COVID-19 outcomes in adults aged 50 years and older: analysis of three prospective cohorts.

Background: Patients with Alzheimer's disease or dementia are at increased risk for COVID-19 hospitalization and mortality. However, no study has examined whether memory function is associated with COVID-19 outcomes in general older adults.

Methods: Data were obtained from SHARE (the Survey of Health, Ageing and Retirement in Europe), HRS (the Health and Retirement Study), and ELSA (the English Longitudinal Study of Ageing), three prospective and representative cohorts of non-institutionalized adults aged 50 years and older in 25 European countries plus Israel, the United States, and the United Kingdom, respectively.

The acute myeloid leukemia microenvironment impairs neutrophil maturation and function through NFκB signaling.

Acute myeloid leukemia (AML), an aggressive hematological malignancy, is driven by oncogenic mutations in stem and progenitor cells that give rise to AML blasts. While these mutations are well-characterized, their impact on healthy hematopoiesis-those blood cells exposed to AML but not mutated-has not been well-characterized. As the marrow is the major site for granulopoiesis, neutrophils are heavily influenced by AML pathobiology.

Recent Advances on Natural Pigment Indigoidine: Occurrence, Biosynthetic Approaches, and Applications.

Indigoidine, a natural biopigment with a planar conjugated structure comprising two pyrrolidinone rings linked by a central double bond, exhibits unique chromogenic properties. Growing environmental concerns over synthetic and plant-derived pigments have driven interest in microbial pigments as sustainable alternatives. Despite the potential of nonribosomal peptide synthetases (NRPSs) to optimize indigoidine biosynthesis, mechanistic insights into their catalytic functions remain limited.

Independent Association of Sleep Apnea-Specific Hypoxic Burden and Sleep Breathing Impairment Index with Thyroid Function in Obstructive Sleep Apnea: A Retrospective Study.

Purpose: This study aimed to explore thyroid function changes in patients with obstructive sleep apnea (OSA) and analyze the relationships among the sleep apnea-specific hypoxic burden (SASHB), the sleep breathing impairment index (SBII), and the function during different sleep stages.

Methods: This retrospective study included 452 patients with OSA who visited the Second Affiliated Hospital of Xi'an Jiaotong University between August 2017 and March 2024. The severity of OSA was evaluated, grouping patients by their apnea-hypopnea index (AHI), SASHB, SBII, and both SASHB and SBII during non-rapid eye movement (NREM) and rapid eye movement (REM) sleep.

Loss of Elmsan1 in cardiomyocytes leads to age-dependent cardiac dysfunction and reduced lifespan.

Histone deacetylase (HDAC) complexes regulate pathological gene programs during heart disease progression. The recently identified mitotic deacetylase complex (MiDAC), which includes DNTTIP1, ELMSAN1, and HDAC1/2, remains the least characterized among these complexes. ELMSAN1 has been implicated in left ventricular remodeling, and its global deletion in mice leads to heart malformation.

Exercise-Induced Oxygen Desaturation and Heart Rate Response During 6-Min Walk Test Predict Pulmonary Hypertension in Exertional Dyspnea: A Retrospective Cohort Study.

Pulmonary hypertension (PH) is a life-threatening condition frequently associated with exertional dyspnea. It remains diagnostically challenging due to limitations in current screening modalities. While the 6-min walk test (6MWT) has been applied for risk stratification in confirmed PH, its potential role in screening remains unexplored.

The important role of chloroplasts in plant immunity.

In nature, plants are under attack by a range of pathogens. To cope with these pathogens, plants have evolved a sophisticated immune system, including pattern-triggered immunity (PTI) initiated by pattern recognition receptors on the cell surface and effector-triggered immunity (ETI) activated by intracellular nucleotide-binding and leucine-rich repeat receptors. In recent years, increasing evidence has demonstrated that organelles such as the chloroplast play crucial roles in complete activation of plant immunity.

A targeted genetic screen identifies Caenorhabditis elegans genes involved in RNAi-independent antiviral defense.

Cellular organisms are constantly challenged by potentially lethal viral infections and rely on diverse antiviral mechanisms for survival. In several systems, including plants and insects, parallel antiviral pathways provide redundancy, ensuring host protection even if one pathway is compromised by a virus. However, whether such alternative antiviral mechanisms exist in nematodes beyond RNA interference (RNAi) remains largely unexplored.

Incidence, reasons, and mortality risk of ICU readmission in surgical patients: A systematic review and meta-analysis.

Background: This study investigates the incidence, reasons, and mortality risk of ICU readmission in surgical patients.

Methods: Systematic searches of PubMed, Embase, Cochrane, and Scopus were conducted for studies from January 2000 to August 2024. Included studies reported ICU readmission or in-hospital mortality in adult surgical ICU patients.

TNS4 promotes lymph node metastasis of gastric cancer by interacting with integrin Β1 and inducing the activation of fibroblastic reticular cell.

Lymph node (LN) metastasis of gastric cancer (GC) is one of the important pathways of GC metastasis, indicating the clinical staging and prognosis of patients. To investigate the underlying mechanism during the process of GC-induced LN metastasis, 7 pairs of GC tissues, paracancerous (PC) tissues, GC-positive LN (LN.P) and GC-negative LN (LN.

Prevalence of and factors associated with long COVID among US adults: a nationwide survey.

Background: People with long COVID report prolonged, multisystem involvement and significant disability. This study aimed to determine long COVID prevalence and factors associated with it among US adults using nationally representative data.

Methods: This cross-sectional analysis utilized data from 2022 Behavioral Risk Factor Surveillance System survey, a nationally representative telephone survey conducted among noninstitutionalized adults aged ≥ 18 years residing in the United States.

Dynamic transcriptomics and physiological insights reveal multi-tissue salt adaptation mechanisms in Amaranthus hypochondriacus across stress gradients.

Transcriptomic and physiological analyses identified key salt-responsive pathways and genes in Amaranthus hypochondriacus under 100/250 mM NaCl stress. Soil salinization critically threatens crop productivity, necessitating the exploration of salt-tolerant species. Amaranthus hypochondriacus, recognized as a salt-tolerant grain species, exhibits distinct adaptive mechanisms under moderate (100 mM NaCl) and severe (250 mM NaCl) salinity based on the integrated physiological and multi-tissue transcriptomic analyses.

Effects of hot air drying on muscle quality, volatile flavor compounds, and lipid profiles in sword prawn () based on physicochemical, GC-IMS, and UHPLC-MS/MS-based lipidomics analysis.

The changes in physicochemical index, volatile flavor, and lipid profiles in during hot air drying were studied. As drying time progressed, the , hardness, chewiness, gumminess, peroxide value (PV), and thiobarbituric acid reactive substances (TBARS) of increased gradually, while the and values first increased and then decreased, and springness decreased progressively. Hot air drying caused the myofibrils to widen and become disordered.

Comparative balanced salt solution and 6 ​% hydroxyethyl starch in goal-directed therapy for major abdominal surgery: A systematic review and meta-analysis.

Goal-directed fluid therapy (GDFT) improves postoperative outcomes in various surgeries, but the optimal fluid choice between balanced salt solutions (BSS) and hydroxyethyl starch (HES) remains debated. This meta-analysis compared postoperative outcomes of GDFT using BSS versus 6 ​% HES in elective major abdominal surgery. Comprehensive database searches identified eight RCTs (1739 patients) published between 2000 and 2024.