Prognostic role of Ki-67 in colorectal carcinoma: Development and evaluation of machine learning prediction models.

Background: Ki-67 is a routine test item in clinical pathology departments. However, its prognostic value requires further investigation, especially in the context of research using machine learning (ML), which remains relatively underdeveloped.

Aim: To investigate the prognostic value of Ki-67 in cases of colorectal carcinoma (CRC) and explore the potential application of ML algorithms to predict the Ki-67 index.

AI-Enabled Parallel Assembly of Thousands of Defect-Free Neutral Atom Arrays.

Assembling increasingly larger-scale defect-free optical-tweezer-trapped atom arrays is essential for quantum computation and quantum simulations based on atoms. Here, we propose an AI-enabled, rapid, constant-time-overhead rearrangement protocol, and we experimentally assemble defect-free 2D and 3D atom arrays with up to 2024 atoms with a constant-time cost of 60 ms. The AI model calculates the holograms for real-time atom rearrangement.

Wedelolactone Ameliorates Ischemic Stroke by Inhibiting Oxidative Damage and Ferroptosis via HIF-1α/SLC7A11/GPX4 Signaling.

Purpose: Wedelolactone (Wel), a furocoumarin compound extracted from the medicinal plant . has been shown to exhibit significant neuroprotective effects. However, the potential of Wel to improve ischemic stroke (IS) and the underlying mechanisms remain unclear.

Metastatic mediastinal malignant tumors of gastrointestinal origin with occult primary lesions: a case report.

Cancer of unknown primary origin (CUP), accounting for 3-5% of malignancies, poses significant diagnostic challenges because of the absence of identifiable primary lesions. While common occult primary tumors involve the lung or pancreas, gastrointestinal (GI)-originated mediastinal metastases are exceedingly rare. A 54-year-old male presented with chest tightness and dyspnea.

Modular DNA barcoding of nanobodies enables multiplexed in situ protein imaging and high-throughput biomolecule detection.

Current immunodetection methods using antibody-DNA conjugates enable multiplexed target detection through orthogonal DNA barcodes, but existing conjugation approaches are labor-intensive and often compromise antibody function. Here, we present a modular, site-specific, and cost-efficient DNA tagging strategy - multiplexed and modular barcoding of antibodies (MaMBA). Utilizing nanobodies as modular adaptors, MaMBA enables direct site-specific labeling of off-the-shelf IgG antibodies with a one-component design.

Diapause-induced transgenerational performance of Trichogramma ostriniae exploiting Corcyra cephalonica eggs.

Background: Diapause induction is a crucial method widely used for storage in commercial production of Trichogramma, which may affect biological traits of parasitoids significantly. However, whether the diapause-induced biological changes persist across generations has never been determined. In a preliminary experiment, we found that diapause-exposed females of Trichogramma ostriniae showed a positive effect interpreted as increased oviposition.

Vitamin D Alleviates Osteoarthritis Progression by Targeting Cartilage and Subchondral Bone via Myd88-TAK1-ERK Axis Suppression.

Background: Osteoarthritis (OA) causes irreversible joint damage, but current treatments fail to fully address its complex pathology. Emerging evidence suggests subchondral bone metabolic dysfunction may initiate OA. While vitamin D (VitD) is well-established for bone metabolism regulation in osteoporosis, its therapeutic potential in OA remains unclear despite observational studies suggesting protective effects.

Identification and validation of NETs-related biomarkers in active tuberculosis through bioinformatics analysis and machine learning algorithms.

Introduction: Diagnostic delays in tuberculosis (TB) threaten global control efforts, necessitating early detection of active TB (ATB). This study explores neutrophil extracellular traps (NETs) as key mediators of TB immunopathology to identify NETs-related biomarkers for differentiating ATB from latent TB infection (LTBI).

Methods: We analyzed transcriptomic datasets (GSE19491, GSE62525, GSE28623) using differential expression analysis (|log, FC| ≥ 0.

Experimental and Simulation Study of Mass Transfer Enhancement by a Foam Microporous Layer in Proton-Exchange Membrane Fuel Cells under a Wide Range of Humidity.

Proton-exchange membrane fuel cells (PEMFCs) represent a critical hydrogen-to-electricity conversion technology for clean energy systems. Increasing the power density is a key development priority for broader applications. However, the accumulation of water under high humidity and high current density will limit the mass transfer efficiency of the PEMFC.

Identification and characterization of TCP transcription factor GmTCP670 associated with soybean development.

TCP (TEOSINTEB RANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR) is a plant-specific transcription factor family that is closely associated with plant growth and development. To date, a few studies have been conducted on the TCP family of soybean. To investigate the function of the TCP transcription factor family in soybeans, we conducted a series of experiments and obtained significant results.

Boron Catalysis-Enabled Regioselective Ring-Retentive Hydrosilylation of Methylenecyclopropanes.

Herein, we report the highly regioselective ring-retentive hydrosilylation of methylenecyclopropanes (MCPs) with hydrosilanes by a B(CF) catalyst. The catalytic protocol affords an atom-efficient route for the synthesis of silylcyclopropanes, featuring 100% atom-efficiency, broad substrate scope (42 examples), mild conditions, and high regio-, and chemoselectivity. The current metal-free catalytic hydrosilylation also provides a complementary pathway for the synthesis of structurally diverse organosilicon compounds from MCPs over transition metal-catalyzed variants.

Adaptive AFM image reconstruction through frequency coefficient selection based on block compressed sensing.

Atomic force microscope (AFM) is a valuable instrument for nano-scale imaging. Traditional AFM demands long time to obtain AFM images due to point-by-point imaging. AFM based on compressed sensing (CS-AFM) is able to obtain AFM images by reconstructing from incomplete measurement.

Bifacially Reinforced Self-Assembled Monolayer Interfaces for Minimized Recombination Loss and Enhanced Stability in Perovskite/Silicon Tandem Solar Cells.

Perovskite/silicon tandem solar cells have shown higher power conversion efficiencies (PCEs) than single-junction cells. However, their record PCE still falls short of the theoretical maximum, and their stability is significantly lower than that of crystalline silicon solar cells. These challenges stem from the substantial losses in open-circuit voltage (V) and the instability of wide-bandgap perovskite devices, which are mainly caused by nonradiative recombination and degradation at the heterojunction interfaces, respectively.

Here comes the sun: integration of light, temperature, and auxin during herbaceous plant grafting.

Light and temperature can regulate auxin production which has been recently shown to be key during graft healing, suggesting that abiotic factors may be vital variables for future graft studies. Grafting is an important horticultural tool used to combine advantageous plant traits. Despite its broad usage, the mechanisms that underlie graft healing remain poorly understood.

Lipid-lowering effect of mangiferin through the inhibition of pancreatic lipase activity.

Background: The inhibition of pancreatic lipase activity to reduce dietary lipid absorption is an effective strategy to combat obesity. Mangiferin, a bioactive flavonoid derived from Mangifera indica, exhibits potent lipid-lowering potential. However, although its in vitro inhibitory effect on pancreatic lipase has been identified, the underlying mechanism remains poorly understood.

CPK27 enhances cold tolerance by promoting flavonoid biosynthesis through phosphorylating HY5 in tomato.

Cold stress is a major environmental challenge affecting the production of crops. Calcium-dependent protein kinases (CDPKs/CPKs) are crucial regulators relaying calcium (Ca) signals into cellular stress responses. However, the specific mechanisms of CPKs in regulating cold stress signaling are not well understood.

Dual-Ascent-Inspired Transformer for Compressed Sensing.

Deep learning has revolutionized image compressed sensing (CS) by enabling lightweight models that achieve high-quality reconstruction with low latency. However, most deep neural network-based CS models are pre-trained for specific compression ratios (CS ratios), limiting their flexibility compared to traditional iterative algorithms. To address this limitation, we propose the Dual-Ascent-Inspired Transformer (DAT), a novel architecture that maintains stable performance across different compression ratios with minimal training costs.

Sex differences in seizure presentation in a Dravet syndrome mouse model.

Objectives: Dravet syndrome is an epileptic encephalopathy mostly because of haploinsufficiency of the SCN1A voltage-gated sodium channel subunit. Disease presentation (i.e.

MiR164a-targeted NAM3 inhibits thermotolerance in tomato by regulating HSFA4b-mediated redox homeostasis.

Extreme weather events, including high temperatures, frequently occur and adversely affect crop growth, posing substantial challenges to global agriculture. MicroRNAs (miRNAs) play integral roles in regulating plant growth and responses to various stresses. In this study, we reveal that microRNA164a (miR164a) in tomato (Solanum lycopersicum) is a pivotal element that exhibits a rapid positive response to heat stress (HS) among multiple miRNAs, while its target NO APICAL MERISTEM 3 (NAM3) shows an opposite complementary response.

Biomimetic multilayered polymeric heart valve featuring ultra-thin thickness and exceptional durability.

The transcatheter aortic valve replacement technique has rapidly developed in clinical practice, with its indications expanding to low-risk populations and younger patients. The durability of valve materials has emerged as a critical requirement from a clinical perspective and a research focus in interventional valves. Polymeric heart valves exhibit a promising future because of their enhanced durability and reduced cost.

Integrated Transcriptome Analysis of lncRNA, miRNA, and mRNA Reveals key Regulatory Modules for Polycystic Ovary Syndrome.

Unlabelled: Polycystic ovarian syndrome (PCOS) is the most common reproductive metabolic disorder in women of reproductive age. However, the underlying mechanism is unclear, because the main symptoms vary with age and the pathogenesis is complex and multifactorial. In order to explore the gene expression and regulation networks, and identify potential biomarkers for diagnosis and treatment of PCOS, we conducted whole RNA sequencing of protein-coding genes, lncRNAs, and miRNAs in peripheral blood with case-control design.

Earable Multimodal Sensing and Stimulation: A Prospective Towards Unobtrusive Closed-Loop Biofeedback.

The human ear has emerged as a bidirectional gateway to the brain's and body's signals. Recent advances in around-the-ear and in-ear sensors have enabled the assessment of biomarkers and physiomarkers derived from brain and cardiac activity using ear-electroencephalography (ear-EEG), photoplethysmography (ear-PPG), and chemical sensing of analytes from the ear, with ear-EEG having been taken beyond-the-lab to outer space. Parallel advances in non-invasive and minimally invasive brain stimulation techniques have leveraged the ear's access to two cranial nerves to modulate brain and body activity.

Isolation and Characterization of Getah Virus GD2202 from Mosquitoes in Foshan, China.

Getah virus (GETV) is a vector-borne virus that can proliferate in mosquitoes and be transmitted to host animals through bites. Clinical infection with the virus mainly causes diarrhea and reproductive disorders in pigs and fever, rash, and edema in horses. This caused huge losses to the pig industry and also affected the level of competition for horses.

Multifunctional liposome boosts glioma ferroptosis and immunotherapy through reinforcement of chemo-dynamic therapy strategy.

Glioma remains significant challenging to completely cure by the conventional surgical resection because of its high infiltrative growth properties. Recently, emerging immunotherapy has achieved remarkable success in treating various cancer, but glioma do not benefit from cancer immunotherapy owing to its specific immunosuppressive tumor microenvironment (iTME). Herein, we show the significant improvement of the immunotherapy efficacy for glioma through multifunctional liposome (Lpo@CuSe-GOx).