Exploring 's Therapeutic Effects on Adenomyosis by Inhibiting TNF-α/HIF-1α/IL-17-Driven Inflammatory Cascade: Mechanistic Insights from Target Prediction and Experimental Validation.

Background: Adenomyosis is a chronic inflammatory gynecological disorder closely linked with diminished fertility potential that poses significant challenges in pharmacological management. (Danshen, DS), a traditional Chinese herb with proven anti-inflammatory properties, has shown efficacy in treating chronic inflammatory conditions across multiple organ systems. However, its mechanisms in addressing adenomyosis remain unclear.

Identification of novel causally related genes in adenomyosis: An integrated summary data-based Mendelian randomization study and bioinformatics analysis.

Adenomyosis (AM) is recognized as a complex gynecological and endocrine disorder that contributes to infertility and elevates the risk of pregnancy complications; however, its underlying genetic basis remains unidentified. This study aimed to identify potentially causative genes that may relate to AM. We conducted a summary data-based Mendelian randomization analysis using single nucleotide polymorphisms as an instrumental variable, along with expression quantitative trait loci data from whole blood and uterus as exposures and AM as the outcome.

Incidental finding of a exons 48-55 deletion during prenatal diagnosis.

Background: genetic variants cause a spectrum of phenotypes, from severe progressive proximal muscle weakness and degeneration leading to wheelchair dependence and death from cardiac and/or respiratory failure to very mild muscular phenotypes; very rarely, cases are completely asymptomatic. Few cases have been reported in males carrying deletions who are asymptomatic.

Methods: Family clinical information was collected from the patients.

Study on the influence of topological structures and soft magnetic materials on the performance of high-speed motors.

The high-speed permanent magnet synchronous motor is one of the crucial components of air compressors, where motor efficiency and noise-vibration issues are the main challenges in the design of high-speed motors. Topology and soft magnetic materials commonly constitute the key factors behind the suitable design of high-speed motors that directly affect their performance. This study designs and analyzes four different 25 kW, 95 000 rpm air compressors with two types of topological structures, namely 6-slot 2-pole and 12-slot 4-pole (12S4P), using two different soft magnetic materials, namely thin 15SW1200 silicon sheet and amorphous magnetic alloy AYFA_Z.

Integrated multi-omics analysis and experimental verification reveal the involvement of the PI3K/Akt signaling pathway in myometrial fibrosis of adenomyosis.

Adenomyosis (AM) is characterized as a chronic and progressive disorder with limited therapeutic strategies available. Myometrial fibrosis is a prominent pathological feature of AM, yet the underlying molecular mechanisms remain elusive. The present study conducted a comparative analysis using proteomics and metabolomics to investigate myometrial fibrosis and its underlying mechanisms.

Scientific landscape and visualization analysis of the link between adenomyosis and infertility from 2000 to 2024.

Objectives: Adenomyosis (AM) is a chronic disorder that significantly impacts women's health and quality of life worldwide, particularly by causing progressive impairment in fertility. This study aimed to summarize and visualize the literature concerning AM-associated infertility using scientometric analysis.

Methods: We conducted a literature search in the Web of Science Core Collection (WoSCC) database for "adenomyosis" and "infertility" as topics from 2000 to 2024.

Comprehensive Profiling of Transcriptome and m6A Epitranscriptome Uncovers the Neurotoxic Effects of Yunaconitine on HT22 Cells.

Objective: To explore different mRNA transcriptome patterns and RNA N6-methyladenosine (m6A) alteration in yunaconitine (YA)-treated HT22 mouse hippocampal neuron, and uncover the role of abnormal mRNA expression and RNA m6A modification in YA-induced neurotoxicity.

Methods: HT22 cells were treated with 0, 5, 10, and 50 μM of YA for 72 h to evaluate their viability and GSH content. Subsequently, mRNA-seq and MeRIP-seq analyses were performed on HT22 cells treated with 0 and 10 μM YA for 72 h, and molecular docking was used to simulate interactions between YA and differentially expressed m6A regulators.

Involvement of autophagy in mesaconitine-induced neurotoxicity in HT22 cells revealed through integrated transcriptomic, proteomic, and m6A epitranscriptomic profiling.

Mesaconitine (MA), a diester-diterpenoid alkaloid extracted from the medicinal herb , is commonly used to treat various diseases. Previous studies have indicated the potent toxicity of aconitum despite its pharmacological activities, with limited understanding of its effects on the nervous system and the underlying mechanisms. HT22 cells and zebrafish were used to investigate the neurotoxic effects of MA both and , employing multi-omics techniques to explore the potential mechanisms of toxicity.

Gelsenicine disrupted the intestinal barrier of Caenorhabditis elegans.

Gelsemium elegans Benth. (G. elegans) is a traditional medicinal herb that has anti-inflammatory, analgesic, sedative, and detumescence effects.

[Hemicellulose modification and cell wall genetic improvement in plants].

Hemicellulose, as a primary component of plant cell walls, constitutes approximately one third of cell wall dry matter and ranks as the second abundant renewable biomass resource in the nature after cellulose. Hemicellulose is tightly cross-linked with cellulose, lignin and other components in the plant cell wall, leading to lignocellulose recalcitrance. However, precise genetic modifications of plant cell walls can significantly improve the saccharification efficiency of lignocellulose while ensuring normal plant growth and development.

Tumor Microenvironment Activatable Nanoprodrug System for In Situ Fluorescence Imaging and Therapy of Liver Cancer.

The development of new imaging and treatment nanoprodrug systems is highly demanded for diagnosis and therapy of liver cancer, a severe disease characterized by a high recurrence rate. Currently, available small molecule drugs are not possible for cancer diagnosis because of the fast diffusion of imaging agents and low efficacy in treatment due to poor water solubility and significant toxic side effects. In this study, we report the development of a tumor microenvironment activatable nanoprodrug system for the diagnosis and treatment of liver cancer.

The Application of a Physiologically Based Toxicokinetic Model in Health Risk Assessment.

Toxicokinetics plays a crucial role in the health risk assessments of xenobiotics. Classical compartmental models are limited in their ability to determine chemical concentrations in specific organs or tissues, particularly target organs or tissues, and their limited interspecific and exposure route extrapolation hinders satisfactory health risk assessment. In contrast, physiologically based toxicokinetic (PBTK) models quantitatively describe the absorption, distribution, metabolism, and excretion of chemicals across various exposure routes and doses in organisms, establishing correlations with toxic effects.

Rational construction of reliable fluorescent probes for rapid detection and imaging evaluation of hazardous thiophenol in real-food and biosystems.

Thiophenol (PhSH), a highly reactive aromatic thiol, plays an essential role as a common industrial raw material in food, pesticides, pharmaceuticals, and cosmetics. In this work, we designed and constructed two fluorescent probes CM-PhSH and CM-Ratio-PhSH by a rational strategy. Specifically, coumarin fluorophores with excellent optical properties were modified, and olefinic unsaturated bonds served as reaction sites for the detection of PhSH.

High-fidelity fluorescent probes for visualizing the inhibitory behavior of selenium on cadmium uptake in rice.

Cadmium (Cd), a widespread and highly toxic environmental contaminant, has seriously impacted the growth of rice and the quality of its products. Hence, it is crucial to monitor and employ robust means to reduce Cd levels in rice, and selenium (Se) has been proven to chelate cadmium ion (Cd) in rice with rational use. Herein, for the first time, the reported selenocysteine (Sec) probe NN-Sec and the newly designed Cd probe SCP were chosen as visualization tools to monitor Sec-inhibited Cd uptake in rice.

Late sodium current in synergism with Ca/calmodulin-dependent protein kinase II contributes to β-adrenergic activation-induced atrial fibrillation.

Atrial fibrillation (AF) is frequently associated with β-adrenergic stimulation, especially in patients with structural heart diseases. The objective of this study was to determine the synergism of late sodium current (late ) and Ca/calmodulin-dependent protein kinase (CaMKII)-mediated arrhythmogenic activities in β-adrenergic overactivation-associated AF. Monophasic action potential, conduction properties, protein phosphorylation, ion currents and cellular trigger activities were measured from rabbit-isolated hearts, atrial tissue and atrial myocytes, respectively.

Rationally Constructed Fluorescent Nanosensor for Nitric Oxide Detection and Imaging in Living Cells and Inflammatory Mice Models.

For the early diagnosis and effective evaluation of treatment effects of inflammation, a bioanalytical method is urgently needed to monitor the metabolite nitric oxide (NO) associated with inflammatory diseases. However, developing a reliable detection method with excellent water solubility, biocompatibility, long retention time, and blood circulation is still challenging. In this work, we reported for the first time a host-guest self-assembled nanosensor CTA for the quantitative detection and visualization of NO levels in inflammatory models.

A Review for In Vitro and In Vivo Detection and Imaging of Gaseous Signal Molecule Carbon Monoxide by Fluorescent Probes.

Carbon monoxide (CO) is a vital endogenous gaseous transmitter molecule involved in the regulation of various physiological and pathological processes in living biosystems. In order to investigate the biological function of CO, many technologies have been developed to monitor the level of endogenous CO in biosystems. Among them, the fluorescence detection technology based on the fluorescent probe has the advantages of high sensitivity, excellent selectivity, simple operation, especially non-invasive damage to biological samples, and the possibility of real-time in situ detection, etc.

Rational construction of a robust nanoprobe for highly selective and sensitive nitrite and formaldehyde detection and imaging in real foods.

Nitrite (NO) and formaldehyde (FA) are practice common food hazards, seriously threatening human health. Herein, for the first time a de novo nanoprobe, named MTB, with a single response group exhibiting different optical signals for NO/FA was reported, which had the following characteristics: i) An adamantane-labeled small molecule NI-adH grafted with polycyclodextrin (Poly-β-CD) to form MTB with excellent water-solubility and biocompatibility. ii) O-phenylenediamine (OPD) with photoinduced electron transfer (PET) played both a fluorescence quencher and as NO/FA trappers.

Activated Two-Photon Near-Infrared Ratiometric Fluorescent Nanoprobe for ONOO Detection and Early Diagnosis and Assessment of Liver Injury.

Liver injury poses a serious threat to human health and growing evidence suggests that it is closely associated with a biomarker (peroxynitrite, ONOO). Therefore, considering that the relationship of ONOO levels with the occurrence and development of liver injury disease remains a challenge, an urgent need exists to develop a reliable and robust tool for its visual rapid diagnosis and assessment. Herein, a two-photon near-infrared (TP-NIR) ratiometric fluorescent nanoprobe () based on a fluorescence resonance energy transfer (FRET) strategy was designed, synthesized, and characterized, which had the advantages of good water solubility, low background interference, deep tissue penetration, and high imaging resolution.

Rational construction of a new water soluble turn-on colorimetric and NIR fluorescent sensor for high selective Sec detection in Se-enriched foods and biosystems.

As a naturally occurring amino acid, selenocysteine (Sec) plays a key role in a variety of cellular functions and Se-enriched foods. In this work, a robust water soluble fluorescence turn-on near-infrared (NIR) sensor NIR-Sec was constructed for Sec detection over biothiols in Se-enriched foods. Specifically, NIR-Sec contains a readily prepared water soluble NIR dicyanoisophorone fluorophore and a well-known response-site 2,4-dinitrobenzenesulfonyl moiety with strong intramolecular charge transfer (ICT) effect to quench the fluorescence intensity of NIR fluorophore.

Molecular engineering-based a dual-responsive fluorescent sensor for sulfur dioxide and nitric oxide detecting in acid rain and its imaging studies in biosystems.

Sulfur dioxide (SO) and nitric oxide (NO), known as sulfur oxides and nitrogen oxides, are toxic air pollutants and seriously threaten human health. Herein, for the first time, a robust dual-response fluorescent sensor CGT with two different emission fluorophores and dual well-known response-group for visual bisulphites (HSO) and nitrites (NO) detection was reported. Specifically, once CGT was incubated with HSO firstly, the color of the test solution changed to dark yellow with no-fluorescence emission, following added NO, the color of the test solution changed to yellow with a bright cyan emission.

Molecular engineering for construction of a novel ONOO- activated multicolor fluorescent nanoprobe for early diagnosis and assessing treatment of arthritis in vivo.

Early diagnosis and assessment of the therapeutic effect of arthritis requires a reliable bioanalytical method to quantitatively and selectively detect the biomarkers peroxynitrite (ONOO) in inflammatory diseases. Compared with previously reported probes for the specific detection of ONOO, molecular engineering based on ONOO-activated multicolor fluorescence nanoprobes will have the advantages of providing multi-channel information and be more suitable for bioimaging in multicomponent complex environments. Herein, for the first time, a fluorescent nanoprobe (CSU-FT) based on fluorescence resonance energy transfer (FRET), which can be activated by ONOO, was constructed for multicolor fluorescence imaging, diagnosis and treatment of arthritis in inflammatory mice.