Allogenic bone marrow-derived mesenchymal stem cells and its conditioned media for repairing acute and sub-acute peripheral nerve injuries in a rabbit model.

The present study evaluated healing potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) and BM-MSCs-conditioned medium (BM-MSCs-CM) for acute and subacute injuries in the rabbit peripheral nerve injury model. The regenerative capacity of MSCs was evaluated in 40 rabbits divided into eight groups, four groups each for acute and subacute injury models. BM-MSCs and BM-MSCS-CM were prepared by isolating allogenic bone marrow from the iliac crest.

The use of circular and hybrid external skeletal fixation systems to repair open tibial fractures in large ruminants: a report of six clinical cases.

A prospective clinical study consisting of six bovines (200-300 kg body weight) undergoing open tibial fractures was carried out, to evaluate the use of circular and hybrid external skeletal fixation (ESF) systems for open tibial fracture repair in large ruminants. The ESF systems consisting of 4 full rings (n = 2), 8 half-rings (n = 1), 2 full rings with 2 sidebars (n = 2) or 2 full rings with 4 sidebars (n = 1) were used to treat open tibial fractures. The healing progress of the animals was evaluated based on different clinical and radiographic examinations.

Evaluation of canine bone marrow-derived mesenchymal stem cells for experimental full-thickness cutaneous wounds in a diabetic rat model.

Background: The wound healing potential of canine bone marrow-derived mesenchymal stem cells (BMSCs) was evaluated in the excisional wound of streptozotocin-induced diabetic rats.

Research Design And Methods: Xenogenic BMSCs were collected aseptically from the iliac crest of healthy canine donors under general anesthesia. Full-thickness experimental wounds (20 × 20 mm) on the dorsum of forty-eight adult healthy Wistar white rats.

Development of a novel atrophic non-union model in rabbits: A preliminary study.

Background And Aim: The currently available atrophic non-union models rely on wide segmental excision of bone diaphysis to impede the process of healing but lack resemblance to the clinical scenario. The present study focused on developing an model of atrophic non-union fracture in rabbit radius that can replicate the clinical scenario.

Materials And Methods: The atrophic non-union fracture model was developed by creating a 10 mm segmental bone defect in the radial diaphysis of five adult New Zealand White rabbits.

Classification and coding of platelet-rich plasma derived from New Zealand white rabbits for tissue engineering and regenerative medicine applications.

Background And Objective: Platelet-rich plasma (PRP) is a category of platelet concentrate that has been widely used as a therapeutic modality in musculoskeletal medicine. The present study was conducted to classify and code the non-activated platelet-rich plasma (nPRP) derived from New Zealand white rabbits for tissue engineering and other regenerative medicine applications.

Methods: PRP was prepared from the whole blood by double centrifugation protocol using a laboratory centrifuge.

Standardization and characterization of adipose-derived stromal vascular fraction from New Zealand white rabbits for bone tissue engineering.

Background And Aim: Adipose tissue-derived stromal vascular fraction (SVF) contains a heterogeneous cell population comprising multipotent adipose-derived stem cells. Regenerative therapy using adipose-derived SVF has broad applications in bone tissue engineering due to the superior osteogenic potential of SVF. This study was designed to standardize and characterize adipose-derived SVF obtained from New Zealand white rabbits for bone tissue engineering and other potential applications.

Biomechanical properties of a novel locking compression plate to stabilize oblique tibial osteotomies in buffaloes.

Objective: Quantitation of mechanical properties of a novel locking compression plate (LCP) for bovine tibia.

Study Design: Prospective in vitro study.

Sample Population: Adult Murrah buffalo (250-350 kg) tibiae.

Clinical evaluation following the percutaneous transplantation of allogenic bone marrow-derived mesenchymal stem cells (aBM-MSC) in dogs affected by vertebral compression fracture.

Stem cell therapy has been extensively evaluated for its potential in managing neuronal diseases and disorders. The present study was performed to evaluate the therapeutic potential of allogenic bone marrow-derived mesenchymal stem cells (aBM-MSC) for the management of neural defects associated with vertebral compression fracture (VCF) in canine. Six clinical cases presented with the history of neural defects secondary to non-deviating VCFs were included in the present study.

Allogeneic mesenchymal stem cells and growth factors in gel scaffold repair osteochondral defect in rabbit.

An attempt was made to improve osteochondral healing with allogeneic mesenchymal stem cells (MSCs) along with certain growth factors. Induced knee osteochondral defects were filled as: phosphate buffer saline (group A); MSCs in collagen gel (group B); group B plus insulin like growth factor-1 (group C); group C plus transforming growth factor β-1 (group D). Gross and scanning electron microscopy showed superior morphology and surface architecture of the healed tissue in groups D and C.

Epoxy-Pin External Skeletal Fixation for Management of Open Bone Fractures in Calves and Foals: A Review of 32 Cases.

Objective:  The aim of this study was to evaluate epoxy-pin external skeletal fixation technique for the treatment of open fractures in calves and foals.

Study Design:  Twenty-eight calves and four foals (weighing 45-105 kg) with fractures distal to the stifle or elbow made the subject for the retrospective study. The pins (2.

Mesenchymal stem cells with IGF-1 and TGF- β1 in laminin gel for osteochondral defects in rabbits.

Objective: Healing of articular cartilage is still a challenge due to its limited potential to regenerate. In the present study, we evaluated allogenic bone marrow mesenchymal stem cells (BM-MSCs) alone or in combination with growth factors, insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1) in laminin scaffolds for healing of osteochondral defects.

Design: Osteochondral defects of 4mm (diameter) x 5mm (depth) were induced in the rabbit knee joints and treated with phosphate-buffered saline (PBS; control), BM-MSCs, BM-MSCs in laminin, BM-MSCs in laminin with IGF-1, or BM-MSCs in laminin with IGF-1 and TGF-β1 in 10 animals each.

Guinea pigs as an animal model for sciatic nerve injury.

The overwhelming use of rat models in nerve regeneration studies is likely to induce skewness in treatment outcomes. To address the problem, this study was conducted in 8 adult guinea pigs of either sex to investigate the suitability of guinea pig as an alternative model for nerve regeneration studies. A crush injury was inflicted to the sciatic nerve of the left limb, which led to significant decrease in the pain perception and neurorecovery up to the 4 weak.

Comparative evaluation of in vitro mechanical properties of different designs of epoxy-pin external skeletal fixation systems.

Objectives: To compare in vitro biomechanical properties of different designs of epoxy-pin external skeletal fixator (ESF) constructs.

Study Design: Mechanical testing study.

Sample Population: Four epoxy-pin ESF design constructs (uniplanar [EU], multiplanar-I [EM-I], multiplanar-II [EM-II], and circular [EC]) were mechanically tested in compression, bending, and torsion.

Effect of IGF-1 and Uncultured Autologous Bone-Marrow-Derived Mononuclear Cells on Repair of Osteochondral Defect in Rabbits.

Objective: To investigate the utility of bone-marrow-derived mononuclear cells (BMNCs) and insulin like growth factor-1 (IGF-1) in articular cartilage repair.

Design: An osteochondral defect of 3 mm diameter and 5 mm depth was created in patellar groove of the left knee joint in each of 36 New Zealand White rabbits. The defect was filled with RPMI-1640 medium in group A (control), autologous BMNCs in group B, and autologous BMNCs plus IGF-1 in group C (n = 12).

Clinicophysiological and haemodynamic effects of fentanyl with xylazine, medetomidine and dexmedetomidine in isoflurane-anaesthetised water buffaloes (Bubalus bubalis).

The present study was undertaken to investigate the sedative, analgesic and clinical effects of xylazine, medetomidine and dexmedetomidine with fentanyl as pre-anaesthetics in water buffaloes and to compare the dose-sparing effect of xylazine, medetomidine and dexmedetomidine on thiopental for induction and isoflurane for maintenance of anaesthesia in water buffaloes. Six male water buffaloes randomly received intravenous fentanyl (5.0 µg/kg body weight) and xylazine (0.

Management of tibial fractures using a circular external fixator in two calves.

Objectives: To report the repair of tibial diaphyseal fractures in 2 calves using a circular external skeletal fixator (CEF).

Study Design: Clinical report.

Animals: Crossbred calves (n=2; age: 6 months; weight: 55 and 60 kg).

Effect of bupivacaine on epidural analgesia produced by xylazine or medetomidine in buffaloes (Bubalus bubalis).

Objective: To evaluate and compare the effect of epidural bupivacaine on analgesia produced by epidural xylazine or medetomidine in buffaloes.

Study Design: Prospective, blinded study.

Animals: Ten male buffalo calves (6-8 months of age; body weight 70-90 kg) were used on two occasions to conduct a total of 20 investigations.

Evaluation of two dynamic axial fixators for large ruminants.

Objectives: To evaluate healing of a radial osteotomy repaired by application of dynamic axial fixation devices (DAF) in large ruminants.

Study Design: In vivo study of bone healing after application of 2 DAF types. Model I had 2 sidebars, each with a central cylindrical cuff (internally threaded) with 2 detachable connecting rods telescoping within the cuff.

In vitro biomechanical properties of linear, circular, and hybrid external skeletal fixation devices for use in large ruminants.

Objective: To report the biomechanical properties of 3 external skeletal fixation (ESF) devices for use in large ruminants.

Study Design: In vitro biomechanical testing of ESF constructs.

Sample Population: Adult buffalo (weighing, 250-350 kg) tibiae (n=27).