PIEZO1-activated donor lymphatic remodeling integrates with autologous lymphangiogenesis to enhance vein graft patency in mice.

The great saphenous vein is the most commonly used conduit in coronary artery bypass grafting; however, the high vein graft failure rates remain an unresolved issue. Here, we compared different harvesting techniques for mouse vein grafts and found that the construction of a denser lymphatic network contributed to improved vein graft patency. Dual lymphatic tracing strategies using -Cre;R26-tdTomato/zsGreen mice uncovered a heterogeneous lymphatic network in mouse vein grafts.

Impact of IK Channel on CD34 Cells in Angiotensin II-Induced Arteriole Remodeling of Hypertensive Mice.

Background: Mechanisms of endothelial repair in hypertension remain unclear. CD34 cells are reported to contribute to vascular regeneration; however, their origin and regulation in hypertension are poorly understood. We investigated the role of IK channels in CD34 cell-mediated endothelial repair during Ang II (angiotensin II)-induced arteriole remodeling.

UDP-glycosyltransferases alleviate the toxic effects of deoxynivalenol on the growth performance and gut damage of Kunming mice.

The purpose of this study was to assess the effects of UDP-glycosyltransferases (UGTs) on alleviating the toxic effects of deoxynivalenol (DON) on Kunming mouse growth performance and gut damage. In this study, a total of 60 3-week-old male Kunming mice were randomly divided into 4 groups and fed the following dietary and drug treatments for 7 weeks: CON, basal diet; CTX, basal diet with i.p.

Lactate levels and the modified age-adjusted quick sequential organ failure assessment (qSOFA) score are fair predictors of mortality in critically ill pediatric patients.

Introduction: This study aimed to evaluate the predictive accuracy of baseline lactate levels and the modified age-adjusted quick Sequential Organ Failure Assessment (qSOFA) score in forecasting mortality in critically ill pediatric patients.

Methods: A retrospective single-center analysis was conducted on patients aged ≤18 years admitted to the intensive care unit (ICU) between January 1, 2019, and December 31, 2023. The predictive performance of lactate levels at baseline and that of the modified age-adjusted qSOFA score were compared in terms of mortality.

CD34 PI16 fibroblast progenitors aggravate neointimal lesions of allograft arteries via CCL11/CCR3-PI3K/AKT pathway.

Transplant-accelerated arteriosclerosis is a common complication that limits the long-term survival of organ transplant recipients. While previous studies have indicated the involvement of CD34 stem/progenitor cells (SPCs) in this process, their heterogeneity and potential adverse effects remains incompletely understood. To investigate the role of CD34 SPCs in transplant arteriosclerosis, we used various genetically modified mouse models, including BALB/c, C57BL/6J, CD34-CreER, Rosa26-tdTomato, Rosa26-iDTR, CD34-Dre, PI16-CreER, and CAG-LSL-RSR-tdTomato-2A-DTR mice.

Electrochemically Enabled Hydroxyphosphorylation of 1,3-Enynes to Access Phosphinyl-Substituted Propargyl Alcohols.

Catalytic difunctionalization with the direct activation of (O)P-H bonds has been recently established as a potentially robust platform to generate valuable organophosphorus compounds. In terms of 1,3-enynes, despite of the various catalytic methods developed for hydrophosphorylation, the radical-mediated hetero-functionalization of two different atoms has been less explored. In this study, we disclosed an electrochemically induced hydroxyphosphorylation of 1,3-enynes for the construction of phosphinyl-substituted propargyl alcohols.

Neutrophil extracellular traps promoting fibroblast activation and aggravating limb ischemia through Wnt5a pathway.

Although the formation of NETs contributes to cancer cell invasion and distant metastasis, its role in the pathological progression of limb ischemia remains unknown. This study investigated the functional significance of NETs in cell-cell crosstalk during limb ischemia. The changes of cell subsets in lower limb ischemia samples were detected by single-cell RNA sequencing.

Neutrophil extracellular traps induced by IL-1β promote endothelial dysfunction and aggravate limb ischemia.

Vascular inflammation and endothelial dysfunction contribute to vascular diseases. While neutrophil extracellular traps (NETs) participate in some vascular pathologies, their roles in lower limb ischemia remain poorly defined. This study investigated the functional significance of NETs in vascular inflammation and remodeling associated with limb ischemia.

Electro-Triggered Cascade Cyclization to Access Phosphinyl-Substituted -Containing Heterocycles.

An electro-triggered cascade cyclization strategy was disclosed with concomitant phosphinylation and -heterocycle construction. It provides a novel and environmentally friendly approach to access phosphinyl-substituted -heterocycles with no external metal catalyst, oxidant, or heating. Mechanistic studies have revealed that anodic oxidation of H-phosphorus compounds occurs first to generate the key P-centered radical directly and cathodic reduction leads to the concurrent release of molecular hydrogen or methane.

Correlation between oncogene integrator complex subunit 7 and a poor prognosis in lung adenocarcinoma.

Background: Lung adenocarcinoma (LUAD) is one of the most common types of cancer worldwide with high incidence and mortality rates. The integrator complex subunit 7 (INTS7) encodes a subunit of the integrator complex that mediates small-nuclear ribonucleic acid (RNA) processing and has been shown to be associated with RNA polymerase II. However, the clinical significance of INTS7 in LUAD is still not clear and needs to be investigated.

The Impact of Stem/Progenitor Cells on Lymphangiogenesis in Vascular Disease.

Lymphatic vessels, as the main tube network of fluid drainage and leukocyte transfer, are responsible for the maintenance of homeostasis and pathological repairment. Recently, by using genetic lineage tracing and single-cell RNA sequencing techniques, significant cognitive progress has been made about the impact of stem/progenitor cells during lymphangiogenesis. In the embryonic stage, the lymphatic network is primarily formed through self-proliferation and polarized-sprouting from the lymph sacs.

The Effect of Lymphangiogenesis in Transplant Arteriosclerosis.

Background: Transplant arteriosclerosis is a major complication in long-term survivors of heart transplantation. Increased lymph flow from donor heart to host lymph nodes has been reported to play a role in transplant arteriosclerosis, but how lymphangiogenesis affects this process is unknown.

Methods: Vascular allografts were transplanted among various combinations of mice, including wild-type, -CreER;R26-tdTomato, -Cre-tdTomato, severe combined immune deficiency, , , and / mice.

Altered Liver Metabolism, Mitochondrial Function, Oxidative Status, and Inflammatory Response in Intrauterine Growth Restriction Piglets with Different Growth Patterns before Weaning.

Frequent occurrence of intrauterine growth restriction (IUGR) causes huge economic losses in the pig industry. Accelerated catch-up growth (CUG) in the early stage of life could restore multiple adverse outcomes of IUGR offspring; however, there is little knowledge about this beneficial phenomenon. We previously found that nutrient absorption related to intestinal function was globally promoted in CUG-IUGR piglets before weaning, which might be the dominant reason for CUG, but what this alteration could lead to in subsequent liver metabolism is still unknown.

CD34 cell atlas of main organs implicates its impact on fibrosis.

Rationale: CD34 cells are believed being progenitors that may be used to treat cardiovascular disease. However, the exact identity and the role of CD34 cells in physiological and pathological conditions remain unclear.

Methods: We performed single-cell RNA sequencing analysis to provide a cell atlas of normal tissue/organ and pathological conditions.

Restored intestinal integrity, nutrients transporters, energy metabolism, antioxidative capacity and decreased harmful microbiota were associated with IUGR piglet's catch-up growth before weanling.

Background: Intrauterine growth restriction (IUGR) is a major inducer of higher morbidity and mortality in the pig industry and catch-up growth (CUG) before weanling could significantly restore this negative influence. But there was limited knowledge about the underlying mechanism of CUG occurrence.

Methods: Eighty litters of newborn piglets were divided into normal birth weight (NBW) and IUGR groups according to birth weight.

The Selenium Yeast vs Selenium Methionine on Cell Viability, Selenoprotein Profile and Redox Status JNK/ P38 Pathway in Porcine Mammary Epithelial Cells.

Comprehensive studies have been conducted to compare the effect of organic and inorganic selenium previously, but there is still limited knowledge about the difference between organic selenium (Se) from varied sources despite the widely use of organic Se in both animal and human being nutrient additives. In the present study, we systemically compared the effect of two different types of organic Se including selenium yeast (SeY) and selenium methionine (Sel-Met) on cell viability, selenoprotein transcriptome, and antioxidant status in porcine mammary epithelial cells (PMECs) and the results indicated that appropriate addition of SeY and Sel-Met both significantly promoted cell viability and up-regulated the mRNA expression of most selenopreoteins including DIOs, GPXs, and TrxRs family et al. ( < 0.

Leucine supplementation during late gestation globally alters placental metabolism and nutrient transport modulation of the PI3K/AKT/mTOR signaling pathway in sows.

In a previously published study we reported that sow dietary leucine supplementation during late pregnancy significantly improved newborn piglet birth weight by stimulating protein synthesis in the longissimus dorsi muscle. However, there is still limited knowledge as to whether leucine can exert its effects on the placenta, one of the most important temporal organs during pregnancy, to promote maternal-fetal nutrient supply and thus contribute to fetal intrauterine development. Therefore, we tested this hypothesis in the present study.

Dietary Extract Supplementation During Late Gestating and Lactating Sows Improves Animal Performance, Nutrient Digestibility, and Manure Ammonia Emission.

This study was conducted to investigate the effect of dietary extract (YSE) supplementation to sow performance, nutrients digestibility and ammonia emission of manure. Total 80 sows were randomly divided into 4 groups and fed with either control, control + 0.06% YSE, control + 0.

A potential new recombinant echovirus 18 strain detected in a 4-year-old child with encephalitis in China in 2019.

The species Enterovirus B includes diverse serotypes that can cause a wide spectrum of human diseases, such as aseptic encephalitis, myocarditis, and paralysis. In this study, a 4-year-old child was diagnosed with viral encephalitis, but the causative agent could not be identified using routine immunological tests. Using metagenomic RNA sequencing, a novel strain of enterovirus B, strain PC06, was identified, and its genome sequence was determined by RT-PCR and Sanger sequencing.

Polynitro-acetone, dimethyl ether, and dimethylamine: a series of potential green and powerful oxidants for propellants.

With the purpose of searching novel, green and energetic oxidants, polynitro-acetone, polynitro-dimethyl ether, and polynitro-dimethylamine are designed as potential powerful oxidants and energetic materials in this work. Their optimized molecular geometries and electronic structures are calculated using density functional theory at m062x/6-311G++(d,p) level. Based on these results, heat of formation (HOF), detonation energy (Q), detonation velocity (D), and detonation pressure (P) are further evaluated.

Effects of cosolvent partitioning on conformational transitions and chain flexibility of thermoresponsive microgels.

The conformational collapse of polymers in mixtures of two individually good solvents is an intriguing yet puzzling phenomenon termed cononsolvency. In this paper, the concept of the preferential adsorption of the cosolvent is combined with mean-field approaches to elaborate the cononsolvency effect of dimethylformamide (DMF) on the thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) microgels in aqueous solutions. We give a quantitative description concerning the effects of DMF preferential adsorption and partitioning on the reentrant transition of PNIPAM microgels below the lower critical solution temperature (LCST) of PNIPAM.

Conformational collapse of spherical poly(N-isopropylacrylamide) brushes under the constraint of bound micelles.

In this paper, we investigate the micelle (charge)-constrained collapse of a spherical poly(N-isopropylacrylamide) (PNIPAM) brush. The system is an example of the transition of a short-length neutral polymer from a stretched state to a folded state under the constraint of long-range electrostatic repulsion. The collapsed state is described as an anisotropic globule comprising a cascade of rod-like or hairpin bundles.

Synergistic Effects of Bound Micelles and Temperature on the Flexibility of Thermoresponsive Polymer Brushes.

The persistence length is a key parameter for the quantitative interpretation of the flexibility of polymers. We have studied complexes composed of a spherical poly(N-isopropylacrylamide) (PNIPAM) brush and a sodium dodecyl sulfate micelle in an effort to characterize the flexibility of tethered PNIPAM below the lower critical solution temperature T. An analytical mean-field model is used to describe the persistence length L in a broad range of ψ, the number of bound micelles per chain.

Nonequilibrium polymer chains induced by conformational transitions in densely interfacial layers.

Nonequilibrium poly(N-isopropylacrylamide) (PNIPAM) chains are generated by temperature-induced conformational transitions in the densely interfacial PNIPAM layer at high adsorbed amounts in aqueous solution. The interleaving chains relax in a spatially heterogeneous space of adsorbed layer which is filled with the domains via the hydrogen bonding and hydrophobic interactions. The relaxation dynamics of interleaving chains is changed from the weekly stretched exponential behaviour at the lower adsorbed amount to the strongly stretched exponential behaviour at the higher adsorbed amount.

Effects of particles on stability of flow-induced precursors.

The effect of two colorant particles with different surface geometries on the stability of shear-induced precursors in isotactic polypropylene was studied after the cessation of shear flow at 140 °C. In the absence of particles, the shear-induced precursors survived for at least 100 s after the shear flow ended. The presence of particles was found to stabilize lower molecular weight chains assisting in the formation of additional shear-induced precursors.