Unraveling the Constrained Cell Growth in Engineered Living Materials.

Engineered living materials (ELMs) leverage the integrative advantages of materials science and synthetic biology for advanced functionalities. Predicting and controlling cellular behavior are essential for designing and building ELMs, requiring a fundamental understanding of the growth dynamics of encapsulated cells. Here, we interrogate the interference of constrained growth with the engineered functionalities and cellular physiology of cyanobacteria and unveil the dynamic interaction between cell growth and spatial confinements within photosynthetic ELMs.

Pathway crosstalk enables degradation of aromatic compounds in marine clade bacteria.

Aromatic compounds are essential raw materials for almost all sectors of human societies but also persistent environmental pollutants recalcitrant to biodegradation. The ocean serves as a significant sink for these compounds, while their biological conversion routes remain poorly understood, hindering a comprehensive understanding of the marine carbon cycle and advancements in bioremediation and biological carbon upcycling. Here, we report the degradation pathway of aromatic molecules in the marine clade bacteria through multi-omics analysis and CRISPR-Cas-based genome editing.

Cation Effect of Benzoate Salts on Conductivity Enhancement of PEDOT:PSS and Their Application in Flexible Organic Light-Emitting Diodes.

Poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) is a widely used flexible electrode material due to its high optical transparency, tunable conductivity, and cost-effective solution processability. However, the excessive insulating PSS content in pristine PEDOT:PSS films leads to an unsatisfactory electrical conductivity and strong acidity. In this work, we introduce a novel class of benzoate salts to significantly enhance the electrical conductivity of PEDOT:PSS by modulating the metal cations.

Photoinduced Copper-Catalyzed Coupling of Acylsilanes with Indoles or Electron-Rich Arenes.

Although the reactions of electrophilic Fischer-type metal siloxycarbenes catalytically generated in situ from acylsilanes with a single nucleophile have been documented, the coupling of acylsilanes with two nucleophiles remains unexplored. Herein, we demonstrate that acylsilanes can act as effective cationic carbyne equivalents under visible-light-driven copper catalysis, enabling their coupling with diverse indoles or electron-rich arenes. This strategy operates under mild, redox-neutral conditions and exhibits broad functional group compatibility.

Learnable prototype-guided multiple instance learning for detecting tertiary lymphoid structures in multi-cancer whole-slide pathological images.

Tertiary lymphoid structures (TLS) are ectopic lymphoid aggregates that form under specific pathological conditions, such as chronic inflammation and malignancies. Their presence within the tumor microenvironment (TME) is strongly correlated with patient prognosis and response to immunotherapy, making TLS detection in whole-slide pathological images (WSIs) crucial for clinical decision-making. Although multiple instance learning (MIL) has shown promise in tumor microenvironment studies, its potential for TLS detection has received limited attention.

Trajectories of metabolic risk factors prior to the onset of cardiovascular disease in patients with newly diagnosed diabetes.

Objectives: Many studies have demonstrated that multiple metabolic risk factors (MRFs) predict cardiovascular disease (CVD) in patients with diabetes, but changes in these markers before CVD onset are not well characterized. We aimed to explore the trajectories of various MRFs before the occurrence of CVD in patients with newly diagnosed diabetes.

Study Design: Prospective cohort study.

Lactylation-driven USP4-mediated ANXA2 stabilization and activation promotes maintenance and radioresistance of glioblastoma stem cells.

Glioblastoma (GBM) is the most primary lethal brain cancer, characterized by the presence of glioblastoma stem cells (GSCs) that initiate and sustain tumor growth and induce radioresistance. Annexin A2 (ANXA2) has been reported to contribute to glioblastoma progression and impart stem cell-like properties to GSCs, however, its post-translational modifications and mechanisms in GSCs maintenance remain poorly understood. Here, we identify that USP4 is preferentially expressed by GSCs in GBM, USP4/ANXA2 supports GSCs maintenance and radioresistance.

Integrated device of luminescent solar concentrators and electrochromic supercapacitors for self-powered smart window and display.

Luminescent solar concentrators are translucent photovoltaic modules potentially used for building window. To store the energy generated by them, a separate energy storage module and voltage regulator module are required, but it is clear that this pairing is unwieldy for application. To address this problem, we propose a "face-to-face" tandem integration of luminescent solar concentrators and electrochromic supercapacitors.

Precision engineering of the probiotic Nissle 1917 with prime editing.

CRISPR-Cas systems are transforming precision medicine with engineered probiotics as next-generation diagnostics and therapeutics. To promote human health and treat disease, engineering probiotic bacteria demands maximal versatility to enable non-natural functionalities while minimizing undesired genomic interferences. Here, we present a streamlined prime editing approach tailored for probiotic Nissle 1917 utilizing only essential genetic modules, including Cas9 nickase from , a codon-optimized reverse transcriptase, and a prime editing guide RNA, and an optimized workflow with longer induction.

One-for-all gene inactivation via PAM-independent base editing in bacteria.

Base editing is preferable for bacterial gene inactivation without generating double-strand breaks, requiring homology recombination, or highly efficient DNA delivery capability. However, the potential of base editing is limited by the adjoined dependence on the editing window and protospacer adjacent motif. Herein, we report an unconstrained base-editing system to enable the inactivation of any genes of interest in bacteria.

Novel Single Perovskite Material for Visible-Light Photocatalytic CO Reduction via Joint Experimental and DFT Study.

Developing advanced and economically viable technologies for the capture and utilization of carbon dioxide (CO) is crucial for sustainable energy production from fossil fuels. Converting CO into valuable chemicals and fuels is a promising approach to mitigate atmospheric CO levels. Among various methods, photocatalytic reduction stands out for its potential to reduce emissions and produce useful products.

Association of impaired fasting glucose with cardiometabolic multimorbidity: The Kailuan study.

Aims/introduction: We investigated the association between impaired fasting glucose (IFG) and cardiometabolic multimorbidity (CMM) in the Chinese population.

Materials And Methods: We included 119,368 participants, free of diabetes mellitus and cardiovascular disease, who participated in the health examination (2006, 2008, 2010) of the Kailuan Study. According to World Health Organization diagnostic criteria, participants were divided into normal fasting blood glucose (FBG) (<6.

Perilipin-2 mediates ferroptosis in oligodendrocyte progenitor cells and myelin injury after ischemic stroke.

JOURNAL/nrgr/04.03/01300535-202507000-00024/figure1/v/2024-09-09T124005Z/r/image-tiff Differentiation of oligodendrocyte progenitor cells into mature myelin-forming oligodendrocytes contributes to remyelination. Failure of remyelination due to oligodendrocyte progenitor cell death can result in severe nerve damage.

Differences in HDL-related coronary heart disease risk between individuals with and without diabetes.

Background And Aims: High-density lipoprotein (HDL) might lose atheroprotective functions in the presence of diabetes. We sought to examine associations of HDL cholesterol (HDL-C) and HDL particle (HDL-P) subclasses with risk of coronary heart disease (CHD) stratified by diabetes.

Methods: We included 393,516 participants (20,691 diabetics and 372,825 nondiabetics) from the UK Biobank.

Oxidative Difunctionalization of -Aryl Bicyclobutyl Amides with Aldehydes: Divergent Synthesis of Acylated and Alkylated 3-Spirocyclobutyl Oxindoles.

An efficient and operationally simple oxidative radical difunctionalization of -aryl bicyclobutyl (BCB) amides with aldehydes is described. It was found that acylated 3-spirocyclobutyl oxindoles were generated from the coupling of BCB-amides and aromatic aldehydes, while reactions gave exclusively decarbonylative alkylarylation products using alkyl aldehydes as radical precursors.

Adherence to a planetary health diet, genetic susceptibility, and incident cardiovascular disease: a prospective cohort study from the UK Biobank.

Background: The influence of adherence to a planetary health diet (PHD) proposed by the EAT-Lancet Commission on cardiovascular disease (CVD) is inconclusive. Besides, whether genetic susceptibility to CVD can modify the association of PHD with CVD remains unknown.

Objective: We aimed to investigate the association between adherence to PHD and CVD, and to evaluate the interaction between PHD and genetic predisposition to CVD.

Blue valorization of lignin-derived monomers via reprogramming marine bacterium .

Biological valorization of lignin, the second most abundant biopolymer on Earth, is an indispensable sector to build a circular economy and net-zero future. However, lignin is recalcitrant to bioupcycling, demanding innovative solutions. We report here the biological valorization of lignin-derived aromatic carbon to value-added chemicals without requesting extra organic carbon and freshwater via reprogramming the marine clade bacterium .

Association of Ultraprocessed Food Consumption with Risk of Cardiovascular Disease Among Individuals with Type 2 Diabetes: Findings from the UK Biobank.

Scope: Among patients with diabetes, who have modified nutritional behavior and a higher risk of cardiovascular disease (CVD), the influence of ultraprocessed foods (UPFs) on CVD remains unknown. The study aims to evaluate the association between UPF intake and the risk of CVD among individuals with type 2 diabetes (T2D) and further examine the potential biological pathways linking the association.

Methods And Results: This study includes 5405 participants with T2D who provided at least one 24-h dietary recall from the UK Biobank study.

Constructing copper Phthalocyanine/Molybdenum disulfide (CuPc/MoS) S-scheme heterojunction with S-rich vacancies for enhanced Visible-Light photocatalytic CO reduction.

Constructing a heterojunction by combining two semiconductors with similar band structures is a successful approach to obtaining photocatalysts with high efficiency. Herein, a CuPc/DR-MoS heterojunction involving copper phthalocyanine (CuPc) and molybdenum disulfide with S-rich vacancies (13.66%) is successfully prepared by the facile hydrothermal method.

Photoredox-Catalyzed Alkylarylation of -Aryl Bicyclobutyl Amides with α-Carbonyl Alkyl Bromides: Access to 3-Spirocyclobutyl Oxindoles.

A visible-light-induced radical alkylarylation of -aryl bicyclobutyl amides with α-carbonyl alkyl bromides for the synthesis of functionalized 3-spirocyclobutyl oxindoles is described in which β-selective radical addition of the alkyl radical to -aryl bicyclobutyl amides forms a key radical intermediate followed by interception with intrinsic arene functional group. This approach can be applicable to a wide range of α-carbonyl alkyl bromides, including primary, secondary, and tertiary α-bromoalkyl esters, ketones, nitriles, and nitro compounds.

Charge transfer in TiO-based photocatalysis: fundamental mechanisms to material strategies.

Semiconductor-based photocatalysis has attracted significant interest due to its capacity to directly exploit solar energy and generate solar fuels, including water splitting, CO reduction, pollutant degradation, and bacterial inactivation. However, achieving the maximum efficiency in photocatalytic processes remains a challenge owing to the speedy recombination of electron-hole pairs and the limited use of light. Therefore, significant endeavours have been devoted to addressing these issues.

A hybrid photocatalytic system enables direct glucose utilization for methanogenesis.

Integration of methanogenic archaea with photocatalysts presents a sustainable solution for solar-driven methanogenesis. However, maximizing CH conversion efficiency remains challenging due to the intrinsic energy conservation and strictly restricted substrates of methanogenic archaea. Here, we report a solar-driven biotic-abiotic hybrid (biohybrid) system by incorporating cadmium sulfide (CdS) nanoparticles with a rationally designed methanogenic archaeon C2A, in which the glucose synergist protein and glucose kinase, an energy-efficient route for glucose transport and phosphorylation from , were implemented to facilitate nonnative substrate glucose for methanogenesis.

Carbon recycling with synthetic CO fixation pathways.

Carbon dioxide (CO) is the node of alleviating global climate change and supporting living organisms on Earth. Currently, the warming climate and the growing population demand enhanced CO fixation for a sustainable future, which stimulates innovations in biotechnology to tackle these challenges. To this endeavor, synthetic biology and metabolic engineering are enabling a promising approach to engineer synthetic carbon fixation in heterotrophic organisms combining the advantages of both autotrophs and heterotrophs.