[Application of lingual wedge shaped coronectomy in extraction of horizontally impacted mandibular third molars].

Purpose: To investigate the effect of lingual wedge shaped coronectomy in extraction of horizontally impacted mandibular third molar(HIMTM).

Methods: A total of 172 patients with HIMTM were randomly divided into experimental group and routine group with 86 cases in each group . Lingual wedge-shaped coronectomy was applied in the experimental group and T-shaped coronectomy was applied in routine group.

[Effects of dynamic nutrition support on energy metabolism, immune function and stress response after oral and maxillofacial tumor surgery].

Purpose: To observe the effect of dynamic nutrition support on postoperative energy metabolism, immune function and stress response in patients with oral and maxillofacial tumors.

Methods: Fifty-six patients with oral and maxillofacial tumor surgery were randomly divided into experimental group and control group (28 in each group). Patients in the experimental group received dynamic enteral and parenteral nutrition support according to the stress period after surgery, ω-3 fish oil fat milk injection and glutamine were added in the nutrition support program.

A preliminary study on the application of bone marrow stromal cell sheet on the formation of functional tissue-engineered bone in dogs.

Purpose: The aim of this study was to construct functional tissue-engineered bone in dogs using cell sheet engineering, a new technique to gain and transfer seed cells.

Materials And Methods: Demineralized bone matrixes, prepared from homologous bone, were coated with recombinant human bone morphogenetic protein-2. Bone marrow stromal cells (BMSCs) were isolated and subcultured.

Single nucleotide polymorphisms in the u-PA gene are related to susceptibility to oral tongue squamous cell carcinoma in the Northern Chinese Han population.

Aim: The purpose of this study was to determine whether susceptibility to oral tongue squamous cell carcinoma (OSCC) is related to polymorphisms in the u-PA gene.

Methods: We examined the rs2227564 C/T and rs2227562 G/A single nucleotide polymorphisms (SNPs) in 196 OSCC patients and 201 age- and gender- matched controls via direct sequencing and PCR-RFLP methods.

Results: Significant differences were found in allelic and genotypic distributions of the rs2227564 and rs2227562 loci when comparing cases and controls.

A dominant CD4(+) T-cell response to Helicobacter pylori reduces risk for gastric disease in humans.

Background & Aims: Immunodominance is an important feature of antiviral, antitumor, and antibacterial cellular immune responses, but it is not well demonstrated in the immune responses against Helicobacter pylori. Antigen-specific CD4(+) T cells protect mice against infection with H pylori. We investigated the immunodominant CD4(+) T-cell response to neuraminyllactose-binding hemagglutinin (HpaA), which is a conserved, H pylori-specific colonization factor that is being investigated as an antigen for vaccination strategies.

[Construction of tissue engineering bone with bone marrow stromal cell sheets].

Objective: To construct tissue engineering bone with bone marrow stromal cell (BMSC) sheets of dogs.

Methods: BMSC were derived from dog bone marrow and cell sheets were prepared with temperature-responsive dishes after the cells were induced by osteogenesis. Allogeneic dogs decalcification bone matrixes (DBM) were prepared.

[An experimental study of endothelial progenitor cells on bone formation in tissue-engineered bone in dogs].

Purpose: To observe the effects of endothelial progenitor cells (EPCs) on bone formation of tissue engineered bone in dogs.

Methods: Bone marrow stromal cells (BMSCs) and EPCs were derived from dog bone marrow and cultured in different medium in vitro. They were seeded on demineralized bone matrix (DBM) to build tissue engineered bone, then the construct was implanted into the fasciae of latissimus dorsi muscle, the degree of bone formation was analyzed with imaging and histological methods at different time points.

[Clinical observation of acupuncture and massage therapy for temporomandibular joint disorders].

Purpose: To explore simple and effective treatment method for temporomandibular joint disorders by investigating 96 temporomandibular joint disorder patients using electroacupuncture pulse stimulation and massage therapy.

Methods: Ninety-six patients with temporomandibular joint disorder including 54 patients with myofacial pain and 42 patients with external pterygoid muscle spasm, were invloved and then divided into electroacupuncture group and massage combined with electroacupuncture group equally and randomly, with 48 cases in each group. Patients in the electroacupuncture group only received electroacupuncture pulse stimulation therapy alone at the acupoints in the affected sides, including Shangguan, Xiaguan, Jiache, Tinggong and Hegu acupoint.

Progress of oral sequelae during head-neck radiotherapy.

Objective: To evaluate xerostomia, mucositis and dental caries during head and neck radiotherapy.

Methods: Twenty patients with nasopharyngeal carcinoma were included. Oral examinations were conducted before radiotherapy, after dosage of 2000 cGy irradiation, immediately after the termination of radiotherapy, and 1 month and 6 months after termination of the radiotherapy.

[Study of bone marrow stroma stem cells cell-sheet and endothelial progenitor cells cultured in vitro on allogenic demineralized bone matrix in mongrel].

Purpose: To investigate the growth situation of bone marrow stroma stem cells(BMSCs) cell-sheet and endothelial progenitor cells (EPCs) on demineralized bone matrix (DBM) and explore the effect of DBM/BMSCs cell-sheet and DBM/EPCs complex on the construction of functional and vascularized tissue-engineered bone in mongrel.

Methods: BMSC cell-sheet was prepared with cell-sheet engineering approach, EPCs were isolated from canine bone marrow and DBM was prepared from homologous bone. BMSCs cell-sheet and EPCs were inoculated on DBM respectively and observed by inverted phase contrast microscope and scanning electron microscope.

[Measurement of mandible bone mineral density of adults in north China].

Objective: To measure the bone mineral density (BMD) of mandible and investigate the relationship between mandible and the whole body skeleton BMD.

Methods: Healthy volunteers were recruited in north China, which were divided into 6 groups by age: > or = 20, > or = 30, > or = 40, > or = 50, > or = 60 and > or = 70 years older, 10 male and 10 female in each group. Dual-energy X-ray absoptiometery (DXA) was used to measure the BMD of the lumbar spine, the mentum of mandible and the mandibular angle.

Effect of platelet-rich plasma and latissimus dorsi muscle flap on osteogenesis and vascularization of tissue-engineered bone in dogs.

Purpose: The present study evaluated the effects of platelet-rich plasma (PRP) and the latissimus dorsi muscle flap on osteogenesis and vascularization of tissue-engineered bone.

Materials And Methods: Bone marrow stromal cells (BMSCs) were subcultured, and PRP was obtained from the same dogs. Demineralized bone matrix (DBM) was prepared from homologous bone.

[A preliminary study on alveolar bone and teeth defect by immediate autologous bone graft and secondary dental implantation].

Purpose: The purpose of this study is to evaluate immediate autologous bone graft combined with secondary dental implant to repair traumatogenic defect and lost teeth.

Methods: 16 patients with labial anterior maxillary alveolar bone defect, caused by trauma for various reasons were selected as the experimental group. The suitable size autologous skull plates or chin bone plates were harvested and transplanted to the bone defect site and fixed with titanium screw immediately after the wound was completely cleaned, and then the gingiva and mucosa were sutured adequately.

[A comparison between CT and SPECT in detecting mandibular invasion by lower gingival squamous cell carcinoma].

Purpose: The purpose of this study is to compare the value of CT and SPECT in diagnosis of lower gingival carcinoma invading the mandible.

Methods: From February 2002 to October 2006, twenty-one patients with lower gingival squamous cell carcinoma were enrolled.The data of CT and SPECT were studied,and compared with histopathological findings.

[Early laser intervention of hemangioma in facial and neck regions of infant].

Objective: The study was to evaluate the method of early laser intervention of hemangioma in facial and neck regions of infant.

Methods: Between January 1999 and December 2006, twelve patients, aged 6 days to 3 months, with cutaneous hemangioma in facial and neck regions, were treated with laser, eight cases with Nd:YAG laser therapy and four cases with Venus laser therapy. Four cases with hemangioma in facial and neck regions of infant treated with oral corticosteroid were as control.

Madelung disease: manifestations of CT and MR imaging.

Objective: The purpose of the present study was to assess the head and neck imaging features of Madelung disease on computerized tomography (CT) and magnetic resonance imaging (MRI), delineate the effects of the fatty deposits on underlying structures, and tabulate the anatomic sites where the neck fat was deposited.

Study Design: Sixteen Chinese patients with Madelung disease in our hospital were reviewed. The CT manifestations of the 16 cases and the MRI manifestations of 1 case were analyzed retrospectively.

[Early treatment of middle ear disease in cleft palate infants].

Objective: To explore the effect of myringotomy with insertion of tube and tympanocentesis on alleviating secretory otitis media (SOM) and hearing loss in cleft palate infants.

Methods: Nineteen cleft lip and palate infants with SOM and hearing loss (38 ears) were treated with myringotomy with insertion of ventilation tube at the same time of repair of the cleft lip, who were performed averagely at 6.9 months of age.

[Establishment and identification of biological characteristics of rat monoclone cell line Rca-B].

Objective: To establish a monoclone cell line of squamous cell carcinoma (SCC) in rat buccal mucosa and to study its biological characteristics.

Methods: SCC in rat oral mucosa was induced by adding 4-nitroquinoline-1-oxide (4NQO) into the SD rats' drinking water, and the cancer cells were then cultured to obtain mixed cells in vitro. The mixed tumor cells were purified by mono cell cloning method.

[Effect of platelet rich plasma on vascularization of tissue-engineered bone].

Objective: To study the effects of platelet rich plasma (PRP) on vascularization of tissue-engineered bone.

Methods: Bone marrow stromal cell (BMSC) were isolated from iliac bone of dogs. PRP was obtained from the same dog and demineralized bone matrix (DBM) was prepared from homologous bone.

Submandibular area metastasis from prostate small cell carcinoma with neuroendocrine differentiation.

Neuroendocrine differentiation in prostatic carcinomas generally confers a more aggressive clinical behavior and less favorable prognosis than usual prostatic carcinomas. In this article, we report a case of a 65-year-old man with prostatic carcinoma who had a metastasis of the submandibular area. His serum prostate-specific antigen level was reduced to below the normal range and carcinoembryonic antigen was increased.

[Effect of platelet-rich plasma and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs].

Objective: To study the effect of platelet-rich plasma (PRP) and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs.

Methods: Bone marrow stromal cells (BMSCs) were isolated from iliac bone of dogs. PRP was obtained from the same dog.

[Study of MSCs in vitro cultured on demineralized bone matrix of mongrel].

Purpose: To manufacture demineralized bone matrix(DBM) of mongrel and to explore the feasibility of DBM as scaffold of bone tissue engineering.

Methods: Thigh bones of mongrel were degreased, demineralized, deproteined, freezed, dried and sterilized to form DBM. Mesenchymal stem cells(MSCs) were seeded onto the scaffold and their growth were examined by inverted phase contrast microscope and scanning electron microscope.

[Madelung disease: CT and MRI findings].

Objective: To investigate the CT and MRI manifestations of Madelung disease.

Methods: The CT and MRI manifestations of 12 cases of Madelung disease were analyzed retrospectively.

Results: The CT and MRI manifestations: cross-sectional CT showed that abnormally proliferated fat mainly deposited in anterior or posterior subcutaneous tissues of the neck, deep into the sternomastoid and trapezius muscles, situated in posterior cervical triangle, around the salivary glands, and excess fat was also found to be situated in supraclavicular fossa, around the paraspinal muscles and larynx, and so on.

[Current application of confocal laser scanning microscope (CLSM) in stomatology].

Confocal laser scanning microscopy is one kind of modern Hi-tech on the basis of confocal imaging which is characterized by depth discrimination capability. It has been widely used in the field of stomatology due to its great advantages of non-destructive and non-invasive optical sectioning and three-dimensional reconstruction of the vital objects, in situ and dynamic real-time observation of the tissues and cells can be performed at high resolution. This paper reviews the fundamentals of confocal imaging and the application of CLSM in the fields of dental material, caries, dentin bonding interface and other basic researches in stomatology in recent years.