A novel C-type lectin, Nattectin-like protein, with the function of regulating AMP expression and phenoloxidase activity in Procambarus clarkii.

As a crucial member of pattern recognition receptors (PRRs), C-type lectins (CTLs) play a pivotal role in innate immunity by recognizing pathogen-associated molecular patterns. In this study, we identified and characterized a novel CTL, Nattectin-like protein (PcNTC), from Procambarus clarkii and conducted a comprehensive functional analysis. PcNTC possesses a carbohydrate recognition domain and a signal peptide.

Phosphorylated Eriocheir sinensis Rab10 regulates apoptosis and phagocytosis to defense Spiroplasma eriocheiris infection.

The Rab GTPases play a crucial role in the regulation of immune responses towards viruses and bacteria infection in invertebrates. The proteomic data revealed Eriocheir sinensis Rab10 (EsRab10) phosphorylation was strongly decreased following Spiroplasma eriocheiris infection. However, the regulatory mechanism by which Rab10 modulates the innate immunity of E.

Eriocheir sinensis CD63 activate mitochondria-mediated apoptosis to resist Spiroplasma eriocheiris infection.

CD63, a member of the tetraspanins, is involved in cell movement, adhesion, immune response. Nevertheless, the role of CD63 in combating pathogen infections in invertebrates remains largely unclear. Tremor disease, whose pathogen is Spiroplasma eriocheiris, is one of the most prevalent illnesses affecting Eriocheir sinensis.

Distribution of phthalate esters and their metabolites in peanut plant during the entire growth period and their dietary risk assessment of peanuts in China.

To understand the remediation potential of peanut plants to phthalate esters (PAEs) contamination, the absorption and accumulation patterns of dibutyl phthalate (DBP), bis (2-ethylhexyl) phthalate (DEHP), and diisononyl ortho-phthalate (DINP), as well as their metabolites-monoalkyl phthalate esters (MPEs), monobutyl phthalate (MBP), monoethylhexyl phthalate (MEHP), and monoisononyl phthalate (MINP), were examined in peanut plant during the entire growth period. It was found that the amounts of DBP and MBP in peanut plants correlated positively, when the DBP content is high, the MBP content is also high, as well as DEHP and MEHP. Additionally, the root contained the highest overall concentrations of DBP, DEHP, DINP, MBP, and MEHP over the course of the growth cycle.

Responses of transcriptome and metabolome in peanut leaves to dibutyl phthalate during whole growth period.

Introduction: The application of agricultural film mulching technology has significantly contributed to increasing crop yield and income, but the pollution caused by residual film has seriously affected agricultural production and the natural environment. Agricultural film is commonly employed to enhance the yield of peanuts; its use may lead to excessive dibutyl phthalate (DBP) residues in peanut kernels. But, limited investigations have been conducted on the regulatory mechanism of peanut leaves in response to DBP exposure throughout the entire growth period.

RNA interference-mediated silencing of ctl13 inhibits innate immunity and development in stored pest Tribolium castaneum.

C-type lectins (CTLs) play a pivotal role in the regulation of insect immunity and growth, making them potential molecular targets for RNA interference (RNAi)-mediated pest control. Although multiple CTLs have been identified in the genomes of various insects, their specific functions and underlying molecular mechanisms remain unclear. In the present study, a novel CTL, Tcctl13 with a single CRD, was identified in Tribolium castaneum.

Characterization and mechanism of simultaneous degradation of aflatoxin B and zearalenone by an edible fungus of GC-Ac2.

Contamination with multiple mycotoxins is a major issue for global food safety and trade. This study focused on the degradation of aflatoxin B (AFB) and zearalenone (ZEN) by 8 types of edible fungi belonging to 6 species, inclulding , , , , and Among these fungi, strain GC-Ac2 was shown to be the most efficient in the degradation of AFB and ZEN. Under optimal degradation conditions (pH 6.

Identification of two galectin-4 proteins (PcGal4-L and PcGal4-L-CRD) and their function in AMP expression in Procambarus clarkii.

Galectins, a family of lectins that bind to β-galactoside, possess conserved carbohydrate recognition domains (CRDs) and play a crucial role in recognizing and eliminating pathogens in invertebrates. Two galectin-4 genes (PcGal4) isoforms, named PcGal4-L and PcGal4-L-CRD, were cloned from the cDNA library of Procambarus clarkia in our study. PcGal4-L contains an open reading frame (ORF, 1089 bp), which encodes a protein consisting of 362 amino acids including a single CRD and six low complexity regions.

Functional Analysis of a CTL-X-Type Lectin CTL16 in Development and Innate Immunity of .

C-type lectins (CTLs) are a class of proteins containing carbohydrate recognition domains (CRDs), which are characteristic modules that recognize various glycoconjugates and function primarily in immunity. CTLs have been reported to affect growth and development and positively regulate innate immunity in . However, the regulatory mechanisms of TcCTL16 proteins are still unclear.

Full-length transcriptome sequencing and comparative transcriptome analysis of in response to infection by the microsporidian .

As a new generation of high-throughput sequencing technology, PacBio Iso-Seq technology (Iso-Seq) provides a better alternative sequencing method for the acquisition of full-length unigenes. In this study, a total of 22.27 gigabyte (Gb) subread bases and 128,614 non-redundant unigenes (mean length: 2,324 bp) were obtained from six main tissues of including the heart, nerve, intestine, muscle, gills and hepatopancreas.

Eriocheir sinensis vesicle-associated membrane protein can enhance host cell phagocytosis to resist Spiroplasma eriocheiris infection.

Vesicle-associated membrane protein (VAMP) belongs to the receptor protein on the membrane of the secretory transport vesicle and involves in host immune function. The intracellular pathogen Spiroplasma eriocheiris could cause Eriocheir sinensis tremor disease. In a previous study, it was found E.

Toxicity of avermectin to Eriocheir sinensis and the isolation of a avermectin-degrading bacterium, Ochrobactrum sp. AVM-2.

Avermectin is widely used in the prevention and treatment of parasites diseases in aquaculture. However, the residual avermectin has a serious impact on the growth and quality of aquatic animals including Eriocheir sinensis. This study shows that the LC of avermectin to E.

Risk assessment of multiple-mycotoxin exposure for consumers of chestnuts in Shandong Province markets in China.

In this study, 321 chestnut samples from Shandong Province in China were analysed for the presence of mycotoxins. We screened for 14 mycotoxins including aflatoxins (AFs: AFB, AFB, AFG, AFG), deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), T-2 toxin (T-2), zearalenone (ZEA), ochratoxin A (OTA), fumonisins (FB, FB, FB), and penicillic acid (PeA). Mycotoxins were detected in 56.

A C-type lectin with dual-CRD from Tribolium castaneum is induced in response to bacterial challenge.

Background: C-type lectins (CTLs), a group of pattern recognition receptors, are involved in regulating the immune response of insects and could be used as potential targets for pest control. However, information about roles of CTLs in the innate immunity of Tribolium castaneum, a serious, worldwide pest that damages stored grain products, is relatively scarce.

Results: Here, a CTL with dual carbohydrate recognition domains (CRDs) containing a highly conserved WHD (Trp -His -Asp ) motif was identified in T.

Toxicity of deltamethrin to Eriocheir sinensis and the isolation of a deltamethrin-degrading bacterium, Paracoccus sp. P-2.

Deltamethrin is used widely in Eriocheir sinensis aquaculture to remove wild fish and parasites. The residual deltamethrin greatly affects the growth and quality of E. sinensis.

A typical C-type lectin, perlucin-like protein, is involved in the innate immune defense of whiteleg shrimp Litopenaeus vannamei.

C-type lectins are a large group of the pattern-recognition proteins, and have been reported to be involved in invertebrate innate immunity, such as cell adhesion, bacterial clearance, phagocytosis, prophenoloxidase activation and encapsulation. Here, a perlucin-like protein (PLP), a typical C-type lectin, was identified from the cDNA library of the shrimp, Litopenaeus vannamei. LvPLP contains a 540 bp open reading frame, encoding a protein of 179 amino acids that includes a single carbohydrate-recognition domain.

Enters Schneider 2 Cells and Relies on Clathrin-Mediated Endocytosis and Macropinocytosis.

causes great economic losses in the crustacean aquaculture industry. However, the mechanism of infecting host cells has been poorly studied. We established a -infected Schneider 2 (S2) cell model and investigated its pathogenic mechanism.

Invasion Into Hemocytes Is Mediated by Pathogen Enolase and Host Lipopolysaccharide and β-1, 3-Glucan Binding Protein.

is a crustacean pathogen, without a cell wall, that causes enormous economic loss. hemocytes are the major targets during infection. As wall-less bacteria, , its membrane protein should interact with host membrane protein directly and firstly when invaded in host cell.

Proteomic and metabolomic responses in hepatopancreas of whiteleg shrimp Litopenaeus vannamei infected by microsporidian Enterocytozoon hepatopenaei.

Enterocytozoon hepatopenaei (EHP) causes hepatopancreatic microsporidiosis (HPM) in shrimp. HPM is not normally associated with shrimp mortality, but is associated with significant growth retardation. In this study, the responses induced by EHP were investigated in hepatopancreas of shrimp Litopenaeus vannamei using proteomics and metabolomics.

Characterization of cathepsin D from Eriocheir sinensis involved in Spiroplasma eriocheiris infection.

Cathepsin D (catD) belongs to a lysosomal aspartic protease superfamily. The full-length catD cDNA from the Chinese mitten crab Eriocheir sinensis (EscatD) was 2748 bp and contained a 1158-bp ORF encoding a protein of 385 amino acids, including a signal peptide and two N-glycosylation sites. Phylogenetic analysis showed that EscatD was clustered into a single group, together with other catD for crustaceans.

The function of serpin-2 from Eriocheir sinensis in Spiroplasma eriocheiris infection.

Serpin families classified serine protease inhibitors regulate various physiological processes. However, there is not study on the role of serpin in immune responses against Spiroplasma eriocheiris as a novel causative pathogen in the Chinese mitten crab, Eriocheir sinensis. In our study, quantitative real-time PCR (qRT-PCR) revealed that the mRNA transcripts of Esserpin-2 were ubiquitous in every tissue, relative higher expression in hepatopancreas, gill and hemocytes, while the intestine, muscle, heart and nerve showed relative lower expression.

Identification and function analysis of ras-related nuclear protein from Macrobrachium rosenbergii involved in Spiroplasma eriocheiris infection.

A ras-related nuclear protein (Ran) protein was obtained from Macrobrachium rosenbergii, named MrRan. Phylogenetic analysis results showed that MrRan was clustered in one group together with other crustaceans. Tissue distribution analysis revealed that MrRan was expressed mainly in gill, intestine and stomach, and expressed weakly in muscle.

Interaction of heat shock protein 60 (HSP60) with microRNA in Chinese mitten crab during Spiroplasma eriocheiris infection.

Heat shock protein 60 from the Chinese mitten crab Eriocheir sinensis (EsHSP60) was previously identified in relation to Spiroplasma eriocheiris infection by isobaric tags for relative and absolute quantitation labelling followed by liquid chromatography-tandem mass spectrometry. In the present study, to validate the immune function of this protein, the cDNA of the EsHSP60 gene was cloned. Various crab tissues were assessed using real-time PCR, which showed that EsHSP60 transcription occurred in all tissues examined.

Adhesin-Like Protein (ALP) Interacts with Epidermal Growth Factor (EGF) Domain Proteins to Facilitate Infection.

is a novel pathogen found in recent years, causing the tremor disease (TD) of Chinese mitten crab . Like infects the newborn mouse (adult mice are not infected) and can cause cataract. Adhesion-related protein is an important protein involved in the interaction between pathogen and host.

A novel C-type lectin is involved in the innate immunity of Macrobrachium nipponense.

C-type lectins (CTLs) play important roles in invertebrate innate immunity by recognizing and eliminating pathogens. In the present study, a low-density lipoprotein receptor class A (LDLa) domain-containing CTL was identified from the oriental river prawn Macrobrachium nipponense, designated as MnCTLDcp1. The full-length cDNA of MnCTLDcp1 was composed of 1462 bp, with a 999-bp ORF encoding a 332-aa protein.