A protein-specific priority code in presequences determines the efficiency of mitochondrial protein import.

The biogenesis of mitochondria relies on the import of hundreds of different precursor proteins from the cytosol. Most of these proteins are synthesized with N-terminal presequences which serve as mitochondrial targeting signals. Presequences consistently form amphipathic helices, but they considerably differ with respect to their primary structure and length.

Polyclonal antibodies towards abrin and ricin-design and potential application for mass spectrometry-based analysis of human biosamples.

The analysis of highly toxic proteins such as abrin and ricin is challenging but comprehensive analytical methods are essential for their unambiguous identification after ingestion. This study pursued three primary aims at detecting abrin and ricin in human biosamples while ensuring that laboratory staff remain protected from direct exposure to these toxic proteins. First, two polyclonal antibodies (pAB) against specific peptides of abrin-A and ricin should be produced.

The importance of distinguishing between natural and managed tree cover gains in the moist tropics.

Naturally regenerated forests and managed tree systems provide different levels of carbon, biodiversity, and livelihood benefits. Here, we show that tree cover gains in the moist tropics during 1982-2015 were 56% ± 3% naturally regenerated forests and 27% ± 2.6% managed tree systems, with these differences in forest type, not only natural conditions (climate, soil, and topography), driving observed carbon recovery rates.

Meeting European Union biodiversity targets under future land-use demands.

The European Union is committed to achieving ambitious area-based conservation and restoration targets in the upcoming decade. However, there is concern that these targets risk conflicting with socioeconomic needs, particularly for food, timber and bioenergy production. Here we develop an integrated spatial planning approach to identify where restoration, conservation and production allocation could maximize benefits to species conservation and climate mitigation, while acknowledging future land demands of the bio-economy.

Narratives for Positive Nature Futures in Europe.

The Nature Futures Framework (NFF) is a novel approach for the development of positive scenarios centered on the relationship of nature and people, emphasizing biodiversity as part of the solution to environmental challenges across various spatial and temporal scales, explicitly addressing a plurality of values for nature. In this work, we describe the process that has led to the formulation of continental-scale positive narratives for conservation in Europe based on the NFF and its value perspectives (Nature for Nature; Nature for Society; Nature as Culture), through an expert group elicitation. We focused on 6 topics in the narratives: Nature Protection and Restoration; Forest Ecosystems; Freshwater Ecosystems; Urban Systems; Agriculture, and Energy.

Same data, different analysts: variation in effect sizes due to analytical decisions in ecology and evolutionary biology.

Although variation in effect sizes and predicted values among studies of similar phenomena is inevitable, such variation far exceeds what might be produced by sampling error alone. One possible explanation for variation among results is differences among researchers in the decisions they make regarding statistical analyses. A growing array of studies has explored this analytical variability in different fields and has found substantial variability among results despite analysts having the same data and research question.

Characterization of the putative yeast mitochondrial triacylglycerol lipase Tgl2.

Mitochondria derive the majority of their lipids from other organelles through contact sites. These lipids, primarily phosphoglycerolipids, are the main components of mitochondrial membranes. In the cell, neutral lipids like triacylglycerides (TAGs) are stored in lipid droplets, playing an important role in maintaining cellular health.

Spatially-explicit land use change emissions and carbon payback times of biofuels under the Carbon Offsetting and Reduction Scheme for International Aviation (CORSIA).

The Carbon Offsetting and Reduction Scheme for International Aviation (CORSIA) requires airlines to offset their greenhouse gas (GHG) emissions above 2019 levels by either buying carbon offsets or using Sustainable Aviation Fuels (SAFs). These are drop-in jet fuels made from biomass or other renewable resources that reduce GHG emissions by at least 10 % compared to kerosene and meet certain sustainability criteria. This study assesses the direct land use change (DLUC) emissions of SAF, i.

An assessment of the state of conservation planning in Europe.

Expanding and managing current habitat and species protection measures is at the heart of the European biodiversity strategy. A structured approach is needed to gain insights into such issues is systematic conservation planning, which uses techniques from decision theory to identify places and actions that contribute most effectively to policy objectives given a set of constraints. Yet culturally and historically determined European landscapes make the implementation of any conservation plans challenging, requiring an analysis of synergies and trade-offs before implementation.

Land potential assessment and trend-analysis using 2000-2021 FAPAR monthly time-series at 250 m spatial resolution.

The article presents results of using remote sensing images and machine learning to map and assess land potential based on time-series of potential Fraction of Absorbed Photosynthetically Active Radiation (FAPAR) composites. Land potential here refers to the potential vegetation productivity in the hypothetical absence of short-term anthropogenic influence, such as intensive agriculture and urbanization. Knowledge on this ecological land potential could support the assessment of levels of land degradation as well as restoration potentials.

A global clustering of terrestrial food production systems.

Food production is at the heart of global sustainability challenges, with unsustainable practices being a major driver of biodiversity loss, emissions and land degradation. The concept of foodscapes, defined as the characteristics of food production along biophysical and socio-economic gradients, could be a way addressing those challenges. By identifying homologues foodscapes classes possible interventions and leverage points for more sustainable agriculture could be identified.

Modelling the probability of meeting IUCN Red List criteria to support reassessments.

Comparative extinction risk analysis-which predicts species extinction risk from correlation with traits or geographical characteristics-has gained research attention as a promising tool to support extinction risk assessment in the IUCN Red List of Threatened Species. However, its uptake has been very limited so far, possibly because existing models only predict a species' Red List category, without indicating which Red List criteria may be triggered. This prevents such approaches to be integrated into Red List assessments.

Radiometal Complexes as Pharmacokinetic Modifiers: A Potent Ga-Labeled Gastrin-Releasing Peptide Receptor Antagonist Based on the Macrocyclic Metal Chelator NODIA-Me.

The gastrin-releasing peptide receptor (GRPr) is overexpressed in various cancer types including prostate and breast carcinomas, making it an attractive target for molecular imaging and therapy. In this work, we designed a novel GRPr antagonistic probe comprising metal chelator NODIA-Me. This 1,4,7-triazacyclononane-based chelator forms positively charged metal complexes due to its neutral methylimidazole arms.

Quantitative Mass Spectrometry Characterizes Client Spectra of Components for Targeting of Membrane Proteins to and Their Insertion into the Membrane of the Human ER.

To elucidate the redundancy in the components for the targeting of membrane proteins to the endoplasmic reticulum (ER) and/or their insertion into the ER membrane under physiological conditions, we previously analyzed different human cells by label-free quantitative mass spectrometry. The HeLa and HEK293 cells had been depleted of a certain component by siRNA or CRISPR/Cas9 treatment or were deficient patient fibroblasts and compared to the respective control cells by differential protein abundance analysis. In addition to clients of the SRP and Sec61 complex, we identified membrane protein clients of components of the TRC/GET, SND, and PEX3 pathways for ER targeting, and Sec62, Sec63, TRAM1, and TRAP as putative auxiliary components of the Sec61 complex.

[Scaphoid fractures : Current diagnostic and treatment concepts].

Scaphoid fractures are by far the most frequent fractures of the carpal bones of the hand and often lead to problematic healing processes if the diagnostics and treatment are inadequate. The main complication of a scaphoid fracture is pseudarthrosis, which leads to carpal collapse and degenerative arthritis of the wrist if left untreated. Early diagnosis and individualized differentiated treatment aim to achieve bony healing with restoration of the scaphoid shape and preservation of the function of the wrist.

Rabconnectin-3α/DMXL2 Is Locally Enriched at the Synaptic Ribbon of Rod Photoreceptor Synapses.

Ribbon synapses reliably transmit synaptic signals over a broad signalling range. Rod photoreceptor ribbon synapses are capable of transmitting signals generated by the absorption of single photons. The high precision of ribbon synapses emphasizes the need for particularly efficient signalling mechanisms.

Tropical dry woodland loss occurs disproportionately in areas of highest conservation value.

Tropical and subtropical dry woodlands are rich in biodiversity and carbon. Yet, many of these woodlands are under high deforestation pressure and remain weakly protected. Here, we assessed how deforestation dynamics relate to areas of woodland protection and to conservation priorities across the world's tropical dry woodlands.

The 3q Oncogene Predicts Response to Neoadjuvant Chemotherapy and Regulates Tumor Cell Migration in Triple Negative Breast Cancer.

In the absence of targeted treatment options, neoadjuvant chemotherapy (NACT) is applied widely for triple-negative breast cancer (TNBC). Response to NACT is an important parameter predictive of oncological outcomes (progression-free and overall survival). An approach to the evaluation of predictive markers enabling therapy individualization is the identification of tumor driver genetic mutations.

Immunohistochemistry Reveals TRPC Channels in the Human Hearing Organ-A Novel CT-Guided Approach to the Cochlea.

TRPC channels are critical players in cochlear hair cells and sensory neurons, as demonstrated in animal experiments. However, evidence for TRPC expression in the human cochlea is still lacking. This reflects the logistic and practical difficulties in obtaining human cochleae.