Publications by authors named "Lize Delanghe"
The spore-forming capacity of Bacillus spp. enables environmental persistence and stable product formulations, yet the interactions of environmental Bacillus spores and vegetative cells with the human immune system are not fully understood. We investigated the immunostimulatory potential of seven environmental Bacillus isolates (B.
View Article and Find Full Text PDFBackground: Chronic infection and inflammation of the lungs contribute significantly to disease progression in persons with cystic fibrosis (pwCF). Treatment regimens are largely based on isolating the putative causative pathogen(s) from respiratory samples using basic culturing methods. While this strategy has shown to be highly valuable in the management of CF, the approach is time-consuming and often misses detection of pathogenic microbes that are more difficult to culture, including Mycobacterium spp.
View Article and Find Full Text PDFThe human skin microbiome plays an essential role in protecting against pathogens and other external substances. This open ecosystem is also influenced by personal and environmental factors, but the precise impact of these factors, such as lifestyle and season, is understudied. We focused here on the inner elbow, a skin site prone to inflammatory conditions like atopic dermatitis and psoriasis.
View Article and Find Full Text PDFArticle Synopsis
- * A study using advanced sequencing techniques found that the skin microbiome in mild AD does not significantly differ in diversity from non-AD patients, and the presence of Staphylococcus aureus remains similar in both groups.
- * However, certain bacteria were found in higher or lower abundances in mild AD lesions, suggesting that understanding these differences could lead to new diagnostic tools and treatments to help manage this condition early on.
Article Synopsis
- Type IV pili (T4P) are structures on the surface of gram-negative bacteria that enable various functions such as attaching to surfaces, forming biofilms, and moving around.
- * A protein called PlzR was found to inhibit the assembly of T4P in Pseudomonas aeruginosa, affecting how the bacteria can be infected by certain bacteriophages.
- * PlzR binds to a T4P chaperone called PilZ, disrupting the proper assembly of T4P by influencing an ATPase named PilB, and its expression is regulated by levels of cyclic di-GMP.
Antimicrobial potential of known and novel probiotics on in vitro periodontitis biofilms.
NPJ Biofilms Microbiomes
January 2023
Several oral diseases are characterized by a shift within the oral microbiome towards a pathogenic, dysbiotic composition. Broad-spectrum antimicrobials are often part of patient care. However, because of the rising antibiotic resistance, alternatives are increasingly desirable.
View Article and Find Full Text PDFDevelopment of a live biotherapeutic throat spray with lactobacilli targeting respiratory viral infections.
Microb Biotechnol
January 2023
Article Synopsis
- Respiratory viruses like influenza and coronaviruses infect the body through the upper respiratory tract, where the resident microbiome plays a protective role.
- The study explores the use of a throat spray containing live lactobacilli probiotics as a potential prevention and treatment method for these viral infections, focusing on their antiviral and immunostimulatory properties.
- Three specific strains of lactobacilli were selected and showed promise by significantly reducing the effects of various respiratory viruses in lab models, while also maintaining their effectiveness when formulated into a throat spray for human testing.
Primary care urgently needs treatments for coronavirus disease 2019 (COVID-19) patients because current options are limited, while these patients who do not require hospitalization encompass more than 90% of the people infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we evaluated a throat spray containing three strains with broad antiviral properties in a randomized, double-blind, placebo-controlled trial. Before the availability of vaccines, 78 eligible COVID-19 patients were randomized to verum ( = 41) and placebo ( = 37) within 96 h of a positive PCR-based SARS-CoV-2 diagnosis, and a per-protocol analysis was performed.
View Article and Find Full Text PDFSelective targeting of skin pathobionts and inflammation with topically applied lactobacilli.
Cell Rep Med
February 2022
Article Synopsis
- Tailored skin microbiome modulation with probiotics, specifically lactobacilli, shows promise in treating acne while being traditionally undervalued in skin health.
- Specific strains of nomadic lactobacilli are selected and microencapsulated for topical use, demonstrating effectiveness in reducing inflammation and lesions in patients with mild-to-moderate acne during a pilot study.
- After 8 weeks of daily application, results indicate a lasting improvement in skin condition, including a decrease in harmful bacteria and a temporary shift in the skin microbiome, suggesting potential immunomodulatory benefits.
Few validated protocols are available for large-scale collection, storage, and analysis of microbiome samples from the vagina, skin, and mouth. To prepare for a large-scale study on the female microbiome by remote self-sampling, we investigated the impact of sample collection, storage, and host DNA depletion on microbiome profiling. Vaginal, skin, and saliva samples were analyzed using 16S rRNA gene amplicon and metagenomic shotgun sequencing, and qPCR.
View Article and Find Full Text PDFCotton and Surgical Face Masks in Community Settings: Bacterial Contamination and Face Mask Hygiene.
Front Med (Lausanne)
September 2021
Article Synopsis
- The study examines bacterial contamination on surgical and homemade cotton face masks during the COVID-19 pandemic, revealing both types harbor significant bacterial loads, with 43% of isolated bacteria showing antibiotic resistance.
- Results indicated distinct microbial profiles between mask types, and the microbiome of wearers' noses and cheeks changed after just 4 hours of wearing a mask.
- Only 21% of surveyed individuals reported daily cleaning of their cotton masks, emphasizing the need for better guidelines on mask hygiene and highlighting the importance of complementary measures like social distancing and ventilation.
Article Synopsis
- The human skin microbiota plays a crucial role in defending against pathogens and regulating immune responses, with lactobacilli identified as beneficial inhabitants of healthy skin.
- Inflammatory skin conditions are linked to an imbalanced skin microbiome, leading to research on lactobacilli-based probiotics for treatment via topical application.
- Lactobacilli support skin health by competing with pathogens, reducing inflammation through their effects on the immune system, and strengthening the skin barrier, though further clinical studies are needed to deepen understanding and confirm efficacy.