Unimolecular Micelle for MLN8237 Delivery to Target AURKA-RalA Crosstalk for Ras-Driven Tumor Suppression in Mice Xenografts.

Targeting Aurora Kinase A (AURKA) to modulate RalA activation offers a promising strategy for tumor suppression in Ras-independent and Ras-dependent cancers. However, clinical use of the AURKA inhibitor MLN8237 (Alisertib) is limited by its hydrophobicity and poor water solubility. To overcome these limitations, here, we developed an enzyme-biodegradable unimolecular micelle (UMM) nanoparticle to deliver MLN8237 (NP) and evaluated its therapeutic efficacy in tumor xenograft models.

CRISPR-Cas9 mediated RALA knockout and reconstitution: insights into the detection and role of RALA S194 phosphorylation in Ras-dependent and Ras-independent cancers.

Downstream of oncogenic RAS, RALA is critical for cancer tumorigenesis, possibly regulated by phosphorylation of its Serine194 residue. We made CRISPR-Cas9 RALA knockout (RALA KO) in three RAS-dependent and two RAS-independent cancer cells. Detection of RALA S194 phosphorylation using the commercial anti-phospho-RALA antibody lacks specificity in all three RAS-dependent cancers.

Optimized prism-based surface plasmon resonance sensor for malaria detection: role of 2D nanomaterials for an enhancement in sensitivity and accuracy.

A prism-based surface plasmon resonance (SPR) sensor is presented for the identification of various phases of malaria. The suggested structure is composed of BK7 prism, copper as metal layer, titanium dioxide (TiO), and tellurium dioxide (TeO) as a dielectric layer, black phosphorous (BP) as 2D material and an analyte layer. The thickness and the layer count of the suggested structure have been optimized for the analysis of angular reflectivity.

A Case-Based Approach to the Management of Corneal Melts and Perforations in Ocular Surface Disorders.

Corneal perforations caused by chronic ocular surface disorders present significant management challenges and can lead to blindness if untreated. This case series reviews the pathophysiology of corneal melts and examines treatment strategies tailored to the size, location, and etiology of perforations in conditions such as Stevens-Johnson syndrome, graft-versus-host disease, and chemical injuries. Through detailed case analyses, various interventions, including cyanoacrylate glue, Tenon's patch grafting, mucous membrane grafts, scleral patch grafts, and conjunctival flaps, were evaluated, along with emerging therapies like biosynthetic hydrogels and collagen-like peptides.

NBS1 from Physcomitrium patens confers resistance against oxidative stress through the BRCA1 C-terminus (BRCT) domain, independent of the MRE11 interaction.

The Nijmegen breakage syndrome 1 (NBS1) protein is a core member of the MRE11-RAD50-NBS1 (MRN) complex and is known to function as a DNA damage sensor within the plant DNA damage response (DDR) pathway. We previously reported that NBS1 from Physcomitrium patens (PpNBS1) also plays a pivotal role in the defense against oxidative damage by reducing the level of cellular reactive oxygen species (ROS). Our study demonstrated that overexpression of PpNBS1 induces the expression of antioxidant genes involved in ROS scavenging in transgenic tobacco plants, leading to reduced accumulation of hydrogen peroxide (HO) and superoxide (O•).

Design, Synthesis, DFT, docking Studies, and antimicrobial evaluation of novel benzimidazole containing sulphonamide derivatives.

In silico approaches have been employed to design a new series of benzimidazole-containing sulphonamide derivatives and qualified compounds have been synthesized to analyze their potential as antimicrobial agents. Antibacterial screening of all synthesized compounds was done using the broth microdilution method against several human pathogenic bacteria, viz. Gram-positive bacteria [B.

In silico and network pharmacology analysis of fucosterol: a potent anticancer bioactive compound against HCC.

The Fucaceae family of marine brown algae includes Ascophyllum nodosum. Fucosterol (FSL) is a unique bioactive component that was identified through GC-MS analysis of the hydroalcoholic extract of A. nodosum.

Enhanced n-butanol production by Clostridium beijerinckii MCMB 581 in presence of selected surfactant.

Extractive butanol fermentation with non-ionic surfactant, a recently explored area, has shown promising results with several advantages but is relatively less investigated. This work reports the extractive fermentation with selected non-ionic surfactants (L62 and L62D) to enhance butanol production using a high-butanol producing strain (Clostridium beijerinckii MCMB 581). Biocompatibility studies with both the surfactants showed growth.