Extending the culture duration could not improve the culture positivity rate and clinical outcomes of periprosthetic joint infection.

Objective: To explore the clinical value of extending the culture time for accurately diagnosing hip or knee PJI.

Methods: This retrospective study included 201 hip or knee PJI patients. All specimens were cultured using a standardized procedure.

The influencing mechanism of thermo-oxidative aging of waste cotton textiles on mechanical properties of their regenerated fibers.

Recycling and reuse of waste cotton textiles reduces environmental pollution and lowers energy consumption. Presently, researchers have conducted a lot of studies on the reuse of waste cotton textiles, but there are fewer studies on how the degree of their aging affects the properties of the regenerated fibers. In particular, the higher the degree of aging, the greater the change of physical and chemical properties of them.

Prosthetic spacers in two-stage revision for knee periprosthetic joint infection achieve better function and similar infection control.

Aims: To explore the clinical efficacy of using two different types of articulating spacers in two-stage revision for chronic knee periprosthetic joint infection (kPJI).

Methods: A retrospective cohort study of 50 chronic kPJI patients treated with two types of articulating spacers between January 2014 and March 2022 was conducted. The clinical outcomes and functional status of the different articulating spacers were compared.

Silk-Fabric Reinforced Silk for Artificial Bones.

Bone implants for different body parts require varying mechanical properties, dimensions, and biodegradability rates. Currently, it is still challenging to produce artificial bones with perfect compatibility with human bones. In this study, a silk-fabric reinforced silk material (SFS) composed of pure silk with exceptional biocompatibility, osteogenesis, and biodegradability is reported, and demonstrates its outstanding performance as a bone implant material.

Genome Characterization of -Positive Isolated From Pigs With Postweaning Diarrhea in China.

Diarrheagenic is the causative agent of diarrhea in infants and animals worldwide. Many isolated strains recovered from pigs with postweaning diarrhea are multidrug resistance (MDR), and hybrids of are potentially more virulent, as enterotoxigenic (ETEC)/Shiga-toxigenic (STEC) hybrids. Here, we used whole-genome sequencing to analyze clinical isolates of the five colistin-resistant .

Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.

Probiotics as an effective and safe strategy for controlling Salmonella infection are much sought after, while autophagy is a central issue in eliminating intracellular pathogens of intestinal epithelial cells. In this study, an animal model of colitis has been developed by infecting weaned pigs orally with a strain of Salmonella Infantis in order to illuminate the potential efficacy of a mixture of Lactobacillus and Bacillus (CBB-MIX) in the resistance to Salmonella infection by regulating butyrate-mediated autophagy. We found that CBB-MIX alleviated S.

Recombinant E-E2 protein vaccine formulated with MF59 and CPG-ODN promotes T cell immunity against bovine viral diarrhea virus infection.

To obtain an effective vaccine candidate against bovine viral diarrhea virus (BVDV) disease which causes great economical loss in cattle industries, recombinant E-E2 protein vaccine containing MF59 and CPG-ODN adjuvants was prepared and assessed in this study. The recombinant plasmid (pET32a-E-E2) was constructed and transformed into BL21 (DE3) cells to produce E-E2 protein. We immunized mice with the MF59-and CPG-ODN-adjuvanted recombinant E-E2 protein, E2 protein, or E protein, respectively.

Lactobacillus johnsonii L531 ameliorates enteritis via elimination of damaged mitochondria and suppression of SQSTM1-dependent mitophagy in a Salmonella infantis model of piglet diarrhea.

Newly weaned piglets challenged with Salmonella infantis were particularly susceptible, whereas oral preadministration of Lactobacillus johnsonii L531 alleviated enteritis and promoted intestinal secretory IgA production. Salmonella infantis-induced activation of NLRC4 and NLRP3 inflammasomes and (nuclear factor kappa B) NF-κB signaling in the small intestine was also inhibited by L. johnsonii L531 pretreatment, thus limiting inflammation.

Lactobacillus johnsonii L531 reduces pathogen load and helps maintain short-chain fatty acid levels in the intestines of pigs challenged with Salmonella enterica Infantis.

In the current study, we screened Lactobacillus strains isolated from the colon of clinically healthy weaned piglets for potential probiotic properties and isolated Lactobacillus. johnsonii L531, which produced high levels of beneficial metabolites (butyric, acetic, and lactic acid) in vitro. We also evaluated the efficacy of this metabolites-producing probiotic in treating Salmonella.

Towards a global phylogeny of freshwater mussels (Bivalvia: Unionida): Species delimitation of Chinese taxa, mitochondrial phylogenomics, and diversification patterns.

The Yangtze River Basin in China is one of the global hotspots of freshwater mussel (order Unionida) diversity with 68 nominal species. Few studies have tested the validity of these nominal species. Some taxa from the Yangtze unionid fauna have not been adequately examined using molecular data and well-positioned phylogenetically with respect to the global Unionida.

GG Affects Microbiota and Suppresses Autophagy in the Intestines of Pigs Challenged with Infantis.

serovar Infantis (. Infantis) is a common source of foodborne gastroenteritis worldwide. Here, GG (LGG) was administrated to weaned piglets for 1 week before .

Reclassification of Lamprotula rochechouartii as Margaritifera rochechouartiicomb. nov. (Bivalvia: Margaritiferidae) revealed by time-calibrated multi-locus phylogenetic analyses and mitochondrial phylogenomics of Unionoida.

The family Margaritiferidae encompasses 12 valid species, which are distributed widely but disjunctively in the Northern Hemisphere. A lack of a well resolved and temporally calibrated phylogenetic framework of Margaritiferidae has made it difficult to discuss the evolutionary pattern and process. Phylogenetic relationships between five major clades, which were revealed in earlier studies, remain elusive and unresolved.

Anti-inflammatory capacity of Lactobacillus rhamnosus GG in monophasic variant Salmonella infected piglets is correlated with impeding NLRP6-mediated host inflammatory responses.

In this study, we investigated the effect of Lactobacillus rhamnosus GG strain (LGG) in ameliorating enteritis in newly weaned pigs following challenge with a monophasic variant of Salmonella enterica serovar Typhimurium (serotyped as 4,[5],12:i:-), which has been linked to disease in humans and livestocks over the past 10 years. In weaned pigs, S. enterica serovar 4,[5],12:i:- induced the mRNA expression of toll like receptor (TLR) 5 and TLR4, while increasing interleukin (IL)-8 and IL-6 mRNA expression in the jejunum.

Oral Administration of GG Ameliorates Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway.

The high rate of serovar Infantis (. Infantis) infection poses significant risk for the development of non-typhoidal gastroenteritis. However, efficient strategies to prevent or treat the infection remain elusive.

Design of an autofocus capsule endoscope system and the corresponding 3D reconstruction algorithm.

A traditional capsule endoscope can only take 2D images, and most of the images are not clear enough to be used for diagnosing. A 3D capsule endoscope can help doctors make a quicker and more accurate diagnosis. However, blurred images negatively affect reconstruction accuracy.

Voice Therapy Effect on Mutational Falsetto Patients: A Vocal Aerodynamic Study.

Objective: The study aims to evaluate the effectiveness of voice therapy (VT) and analyze the vocal aerodynamic characteristics in mutational falsetto (MF) patients.

Methods: From October 2010 through May 2014, 26 patients with MF at Sun Yat-sen Memorial Hospital were studied retrospectively. Vocal assessment, including the 10-item Voice Handicap Index (VHI-10), fundamental frequency (F0), and vocal aerodynamic parameters (subglottic pressure [SGP]), aerodynamic power [AP], mean expiratory airflow, and maximum phonation time [MPT]), was proceeded before and after VT.

Low molecular weight thiols in arsenic hyperaccumulator Pteris vittata upon exposure to arsenic and other trace elements.

Low molecular weight thiol-containing compounds have been reported to play an important role in metal detoxification and accumulation in some higher plants. The formation of these low molecular weight thiols in the recently discovered arsenic hyperaccumulator, Chinese Brake fern (Pteris vittata) upon exposure to arsenic and other trace metals was investigated. In addition to cysteine and glutathione, an unidentified thiol was observed in the plants exposed to arsenic, which was not found in the control.