Prognostic and Predictive Value of Machine Learning-Based Biomarker and Pathomics Signatures in Patients With Prostate Cancer.

Recurrence and the potential development of castration resistance after radical prostatectomy (RP) are significant challenges in the management of prostate cancer (PCa). Despite the development of advanced prognostic models, few have been clinically applied. Five machine learning algorithms (LASSO, RSF, SVM-RFE, Boruta, and XGBoost) were used to identify biomarkers for PCa using transcriptome data from multicenters (TCGA, MSKCC, DKFZ, and GSE70770) for constructing and validating the metastasis-associated prognostic risk score (MAPRS), which revealed the molecular biological heterogeneity and was confirmed with in-house histopathological samples.

Effects of Plasma Aldosterone and Renin Concentrations With Blood Pressure Variation and Hypertension Risk in Chinese Adults: A Population-Based Hakka Biobank Study.

Objectives: The Renin-Angiotensin-Aldosterone System has been implicated in regulating blood pressure (BP), yet its effects on BP-related phenotypes are not well characterized in the Chinese population.

Methods: Based on 8914 participants from the Hakka Biobank cohort of China, regression models, restricted cubic spline, and subgroup analyses were used to analyze associations of plasma aldosterone concentration (PAC), plasma renin concentration (PRC), and the aldosterone-to-renin ratio (ARR) with multiple BP-related phenotypes.

Results: Multivariable regression models indicated that higher log-PAC and log-ARR, and lower log-PRC, were associated with higher systolic blood pressure, diastolic blood pressure, pulse pressure, and mean arterial pressure (all P < .

High-efficiency antioxidant ROS-responsive thermosensitive hydrogel encapsulated Fenofibrate for the treatment of corneal neovascularization.

Corneal neovascularization (CNV) has become one of the common blinding ocular diseases recognized worldwide, affecting millions of people every year. Numerous studies have shown that oxidative stress and inflammation are important factors for the occurrence and development of CNV. In the present study, we established an antioxidant and reactive oxygen species (ROS) responsive thermosensitive hydrogel for long-acting drug delivery, combining with the loaded anti-angiogenic drug to the therapy for CNV.

Disrupted Mitochondrial Dynamics Impair Corneal Epithelial Healing in Neurotrophic Keratopathy.

Neurotrophic keratopathy (NK) is a degenerative corneal disease characterized by impaired corneal sensitivity and epithelial repair that is often linked to sensory nerve dysfunction. To establish a clinically relevant model and explore the mechanisms underlying NK pathogenesis, we developed a novel mouse model through partial transection of the ciliary nerve. This approach mimics the progressive nature of NK, reproducing key clinical features such as corneal epithelial defects, reduced sensitivity, diminished tear secretion, and delayed wound healing.

Enzymatic degradation of polybutylene succinate by recombinant cutinase cloned from Paraphoma chrysanthemicola.

Polybutylene succinate (PBS), a biodegradable plastic, can be used as an alternative to traditional plastics to effectively solve the growing plastic pollution. Although PBS is theoretically completely biodegradable, slow degradation remains a problem in practical applications, leading to the possibility of environmental pollution. In this study, after the PBS degradation ability of the fungus Paraphoma chrysanthemicola was determined, a P.

Thermosensitive Polyhedral Oligomeric Silsesquioxane Hybrid Hydrogel Enhances the Antibacterial Efficiency of Erythromycin in Bacterial Keratitis.

Bacterial keratitis is a serious ocular infection that can impair vision or even cause blindness. The clinical use of antibiotics is limited due to their low bioavailability and drug resistance. Hence, there is a need to develop a novel drug delivery system for this infectious disease.

Constructing lactylation-related genes prognostic model to effectively predict the disease-free survival and treatment responsiveness in prostate cancer based on machine learning.

Prostate cancer (PCa) is one of the most common malignancies in men with a poor prognosis. It is therefore of great clinical importance to find reliable prognostic indicators for PCa. Many studies have revealed the pivotal role of protein lactylation in tumor development and progression.

Identification a novel Ganoderma FIP gene from Ganoderma capense and its functional expression in Pichia pastoris.

Ganoderma capense is a precious medicinal fungus in China. In this study, a novel fungal immunomodulatory protein gene, named as FIP-gca, was cloned from G. capense by homologous cloning.

Molecular characterization and expression analysis of the genes in tomato ( L.).

Remorin (REMs) are plant-specific and plasma membrane-associated proteins that play an essential role in the growth and development of plants and adaptations to adverse environments. To our knowledge, a genome-scale investigation of the genes in tomato has never been systematically studied. In this study, a total of 17 genes were identified in the tomato genome using bioinformatics methods.

Heterologous expression of enhances root development and salt tolerance in .

Introduction: β-Mannanase is a plant cell wall remodeling enzyme involved in the breakdown of hemicellulose and plays an important role in growth by hydrolyzing the mannan-like polysaccharide, but its function in adaptation to salt stress has been less studied.

Methods: Based on cloned the gene from L., the study was carried out by heterologously expressing the gene in , and then observing the plant phenotypes and measuring relevant physiological and biochemical indicators under 150 mM salt treatment.

A New Signature That Predicts Progression-Free Survival of Clear Cell Renal Cell Carcinoma with Anti-PD-1 Therapy.

Immunotherapy has greatly improved the survival time and quality of life of patients with renal cell carcinoma, but the benefits are limited to a small portion of patients. There are too few new biomarkers that can be used to identify molecular subtypes of renal clear cell carcinoma and predict survival time with anti-PD-1 treatment. Single-cell RNA data of clear cell renal cell carcinoma (ccRCC) treated with anti-PD-1 were obtained from public databases, then 27,707 high-quality CD4 + T and CD8 + T cells were obtained for subsequent analysis.

Salvage Surgery for Advanced Lung Adenocarcinoma After Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Treatment.

Background: No published studies to date have evaluated the detailed pathologic and genetic features of lung adenocarcinoma after epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) therapy and salvage surgery. We aimed to evaluate the pathologic and genetic changes of tumors in patients with advanced lung adenocarcinoma treated with EGFR TKI therapy and salvage surgery.

Methods: This study retrospectively collected data from 29 advanced lung adenocarcinoma patients who underwent EGFR TKI therapy, followed by salvage operation, between January 2010 and December 2018.

Novel biomarkers predict prognosis and drug-induced neuroendocrine differentiation in patients with prostate cancer.

Background: A huge focus is being placed on the development of novel signatures in the form of new combinatorial regimens to distinguish the neuroendocrine (NE) characteristics from castration resistant prostate cancer (CRPC) timely and accurately, as well as predict the disease-free survival (DFS) and progression-free survival (PFS) of prostate cancer (PCa) patients.

Methods: Single cell data of 4 normal samples, 3 CRPC samples and 3 CRPC-NE samples were obtained from GEO database, and CellChatDB was used for potential intercellular communication, Secondly, using the "limma" package (v3.52.

COVID-19 inhibits spermatogenesis in the testes by inducing cellular senescence.

COVID-19 (SARS-CoV-2) has been linked to organ damage in humans since its worldwide outbreak. It can also induce severe sperm damage, according to research conducted at numerous clinical institutions. However, the exact mechanism of damage is still unknown.

Identification and validation of a lipid metabolism gene signature for predicting biochemical recurrence of prostate cancer after radical prostatectomy.

Background: Pro5state cancer is one of the most commonly diagnosed cancers in men worldwide and biochemical recurrence occurs in approximately 25% of patients after radical prostatectomy. Current decisions regarding biochemical recurrence after radical prostatectomy are largely dependent on clinicopathological parameters, which are less accurate. A growing body of research suggests that lipid metabolism influences tumor development and treatment, and that prostate cancer is not only a malignancy but also a lipid metabolism disease.

Genome-wide association study reveals the genetic basis of brace root angle and diameter in maize.

Brace roots are the main organ to support the above-ground part of maize plant. It involves in plant growth and development by water absorption and lodging resistance. The bracing root angle (BRA) and diameter (BRD) are important components of brace root traits.

Generation of 2D Airy beams with switchable metasurfaces.

Airy beams exhibit intriguing characteristics, such as diffraction-free propagation, self-acceleration, and self-healing, which have aroused great research interest. However, the spatial light modulator that generates Airy beams has problems such as narrow operational bandwidth, high cost, poor phase discretization, and single realization function. In the visible region (λ∼532 nm), we proposed a switchable all-dielectric metasurface for generating transmissive and reflective two-dimensional (2D) Airy beams.

A Novel Mutation in the FYCO1 Gene Causing Congenital Cataract: Case Study of a Chinese Family.

Congenital cataract is the most important global cause of visual impairment in children. Autosomal dominant and autosomal recessive inheritance account for the majority of the hereditary nonsyndromic congenital cataract. The function of FYCO1 gene is to guide the transport of the microtubule-directed vesicles.

Adjuvant Chemotherapy for Pathological Node-Positive Disease in Squamous Cell Carcinoma of the Esophagus after Neoadjuvant Chemoradiotherapy Plus Surgery.

The purpose of the present study is to determine the impact on survival using adjuvant chemotherapy on patients with locally advanced esophageal cancer. From 2007 to 2016, we enrolled 127 locally advanced esophageal squamous cell carcinoma patients treated with combined neoadjuvant chemoradiotherapy (nCRT) and surgery. For patients with the pathological residual primary disease (pT+) and/or residual node disease (pN+) after nCRT, adjuvant chemotherapy was also given after consideration of the toxicity of nCRT, patient performance, and/or comorbidity.

Biodegradation of polybutylene adipate-co-terephthalate by Priestia megaterium, Pseudomonas mendocina, and Pseudomonas pseudoalcaligenes following incubation in the soil.

Soil that contained polybutylene adipate-co-terephthalate (PBAT) was incubated with Priestia megaterium, Pseudomonas mendocina, and Pseudomonas pseudoalcaligenes to improve the biodegradative process of this polymer. The mixture of Pr. megaterium and Ps.

Screening of early-staged colorectal neoplasia by clonal hematopoiesis-based liquid biopsy and machine-learning.

Liquid biopsy test has a better uptake for colorectal cancer (CRC) screening. However, suboptimal detection of early-staged colorectal neoplasia (CRN) limits its application. Here, we established an early-staged CRN blood test using error-corrected sequencing by comparing clonal hematopoiesis (CH) of 63 CRN patients and that of 32 controls.

Ganoderma immunomodulatory proteins: mushrooming functional FIPs.

Fungal immunomodulatory protein (FIP) is a novel functional protein family with specific immunomodulatory activity identified from several macro-fungi. A variety of biological activities of FIPs have been reported, such as anti-allergy, anti-tumor, mitogenic activity, and immunomodulation. Among all known FIPs, the firstly discovered FIP was isolated from Ganoderma lucidum, and most FIP members were from Ganoderma genus.