The correlation between EGFR mutation status and clinicopathological characteristics, Ki67 expression and immune cell infiltration in lung adenocarcinoma with different radiological subtypes.

Background: To clearly reveal the correlations between epidermal growth factor receptor (EGFR) mutation status and clinicopathological characteristics, Ki67 expression and immune cell Infiltration in lung adenocarcinoma (LUAD) with different radiological subtypes.

Methods: Patients with pathological stage 0-III LUAD who underwent resection and received EGFR detection in our department between July 2019 and May 2023 were retrospectively reviewed. All included patients were divided into four groups based on different consolidation-to-tumour ratio (CTR).

CD24 recruits tumor-associated neutrophils to promote the progression of hepatocellular carcinoma.

Background: The immunosuppressive tumor microenvironment is a significant challenge in the treatment of hepatocellular carcinoma (HCC), necessitating the urgent development of strategies to mitigate its effects.

Methods: The application of bioinformatics methods to predict the expression level of CD24 in HCC and its relationship with the occurrence and development of HCC. Gene-engineered mice and flow cytometry were used to study the immune cell populations regulated by CD24.

Catalytic cooperation between Ru and Pd atoms on hierarchical porous Ce-based oxide for boosting engine exhaust purification.

Simultaneous oxidation of soot and CH emitted from natural gas-diesel dual fuel engine is a new challenge. Herein, a robust catalyst of binary Ru-Pd components supported on three-dimensional ordered macro-mesoporous cerium-zirconium oxide (RuPd/3DOMM-CZO) is elaborately constructed. Ordered macro-mesopore structure in novel hierarchical porous cerium-zirconium oxide can respectively enhance mass transfer of soot and gaseous reactants, and binary Ru-Pd active components can improve activation for NO and CH.

Case Report: Clinical features and management of anti-mGluR1 encephalitis: case illustration and review of the literature.

Background: Anti-metabotropic glutamate receptor 1 (mGluR1) encephalitis is a rare autoimmune disease affecting cerebellar Purkinje cells. Only thirty-nine cases have been reported globally, with inconsistent documentation of treatments and outcomes. A systematic review is needed to identify prognostic factors and expand clinical understanding and treatment options.

CqStathmin negatively regulates white spot syndrome virus infection by destabilizing microtubules in Cherax quadricarinatus.

Stathmin is a microtubule-destabilizing protein that disrupts microtubule dynamics by promoting the depolymerization of microtubules or preventing the polymerization of tubulin heterodimers, thereby performing various cellular functions. In this study, a CqStathmin in the invertebrate crayfish was found to be involved in the infection of white spot syndrome virus (WSSV), a DNA virus susceptible to shrimp. The CqStathmin was identified from the red claw crayfish Cherax quadricarinatus, sharing the conserved domain and phylogenetic relationship with stathmin homologs of other species.

Strategic Design and Synthesis of N, S, P Tridoped Carbon: A Highly Efficient Metal-Free Catalyst for Oxygen Reduction Reaction.

Doping multiple nonmetal heteroatoms into carbon materials is a promising avenue for boosting the catalytic performance of carbon-based metal-free catalysts (CMFCs) for the oxygen reduction reaction (ORR). Nevertheless, selecting appropriate precursors and doping processes to synthesize high-performance CMFCs for the ORR remains challenging. Herein, N-doped carbon (N-C) and N,S-codoped carbon (NS-C) catalysts were first prepared via in situ doping.

hnRNPA2B1 restrains granulosa cell ferroptosis by mA/SLC7A11 in premature ovarian failure.

Premature ovarian failure (POF) is a relatively severe gynecological disorder that is often accompanied by other systemic diseases. Here, this study identified that N-methyladenosine (mA) key enzyme heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2B1) was associated with POF pathophysiological process. In the chemotherapy induced POF animal and cells, hnRNPA2B1 was down-regulated upon cisplatin (CDDP) treatment.

Exploring the comorbidity mechanisms of ITGB2 in rheumatoid arthritis and membranous nephropathy through integrated bioinformatics analysis.

Background: Patients with rheumatoid arthritis (RA) are more likely to comorbid renal diseases, with membranous nephropathy (MN) being the most common. This study aimed to explore the common pathogenesis between RA and MN using integrated bioinformatics analysis.

Methods: Bulk and single-cell RNA sequencing datasets were obtained from the Gene Expression Omnibus and ImmPort databases.

Glucose-induced STUB1-GOT2 axis promotes aspartate synthesis and mitochondrial dysfunction in bladder cancer.

Aberrant glucose metabolism, a characteristic of malignant tumors, contributes to the development and progression of bladder cancer (BCa). However, the underlying mechanism by which aberrant glucose metabolism promotes BCa progression is still incompletely understood. Here, we demonstrate that low levels of STUB1 are associated with worse progression and poor prognosis of BCa patients.

Utilizing shallow features and spatial context for weakly supervised intracerebral hemorrhage segmentation.

Background: In deep learning-assisted intracerebral hemorrhage (ICH) diagnosis, because weak image-level labels cannot provide supervisory information about the target location, weakly-supervised semantic segmentation (WSSS) methods are relatively limited. Therefore, we developed a novel method for improving the ICH segmentation results from weak image-level labels.

Methods: This paper proposes the Shallow-Feature class activation map (CAM) module, which utilizes fine-grained information from the shallow feature maps of convolutional neural networks (CNNs) to generate CAM for accurate target localization and contour.

The Role of APOA-I in Alzheimer's Disease: Bridging Peripheral Tissues and the Central Nervous System.

Lipid metabolism disorders represent a significant risk factor for the pathogenesis of Alzheimer's disease (AD). Apolipoprotein E (APOE) has been regarded as a pivotal regulator of lipid homeostasis in the central nervous system (CNS), with polymorphic alleles identified as genetic risk factors for late-onset AD. Despite advances in APOE research and the development of numerous pharmaceutical approaches targeting distinct APOE isoforms, there remain limited treatment approaches for AD that focus on lipid metabolic homeostasis.

Counterion-Mediated Assembly of Fluorocarbon-Hydrocarbon Surfactant Mixtures at the Air-Liquid Interface: A Molecular Dynamics Study.

This study employs molecular dynamics simulations to investigate counterion effects (Li, Na, K) on the interfacial aggregation of mixed short-chain fluorocarbon, Perfluorohexanoic acid (PFHA), and Sodium dodecyl sulfate (SDS) surfactants. Motivated by the need for greener surfactant alternatives and a fundamental understanding of molecular interactions governing their behavior, we demonstrate that counterion hydration radius critically modulates system organization. K ions induce superior monolayer condensation and interfacial performance compared to Li and Na counterparts, as evidenced by threefold analysis: (1) RMSD/MSD-confirmed equilibrium attainment ensures data reliability; (2) 1D/2D density profiles and surface tension measurements reveal K-enhanced packing density (lower solvent-accessible surface area versus Na and Li systems); (3) Electrostatic potential analysis identifies synergistic complementarity between SDS's hydrophobic stabilization via dodecyl chain interactions and PFHA's charge uniformity, optimizing molecular-level charge screening.

High-efficient hollow porous MnCoO nanoreactors for enhancing catalytic soot oxidation.

Herein, we have elaborately designed and successfully fabricated the high-efficient hollow porous MnCoO nanoreactors (MnCo-HPs) by optimizing the heating decomposition conditions of metal-organic frameworks Mn[Co(CN)]·nHO precursors with a nanocube-like structures. The diameter of the nanopores on the shells of MnCo-HPs is around 10.0 nm, which is beneficial for improving the diffusion of NO and O into the hollow porous cage and enhancing the activation of NO to NO.

Cytoskeletal Proteins and Alzheimer's Disease Pathogenesis: Focusing on the Interplay with Tau Pathology.

The aggregation of Tau protein into neurofibrillary tangles (NFTs), a hallmark of Alzheimer's disease (AD), is associated with cognitive decline. Recent studies have revealed that neuronal cytoskeletal instability drives early AD pathogenesis. The physiological interaction between tau and the microtubule (MT) is crucial for maintaining axonal transport and stability.

The mechanical effect of aligner's thickness and material properties in invisible orthodontics: A quantitative finite element study.

Invisible orthodontics benefits significantly from aligner technology, yet optimizing material properties and thickness for diverse patient needs is a challenge. The aim of this study was to investigate the interaction between key material properties and thickness-and their collective influence on orthodontic treatment outcomes. A three-dimensional model of the tooth, periodontal ligament, and bone complex was constructed, with attachments centered on each tooth crown.

Potential therapeutic targets for deep vein thrombosis: A proteome-wide Mendelian randomization study.

BackgroundThe incidence of deep vein thrombosis (DVT) of the lower extremities is threatening people's health. The development of drug targets for the treatment of DVT is necessary.MethodsWe mainly conducted a proteome-wide Mendelian randomization (MR) study to explore the causal associations of plasma proteins with DVT of the lower extremities.

Ginsenoside Rg5 alleviates radiation-induced acute lung vascular endothelium injury by reducing mitochondrial apoptosis via Sirt1.

Background: Ginsenoside Rg5 possesses potent anti-oxidative, anti-inflammatory, and cytoprotective properties. This study explored the protective effects of ginsenoside Rg5 on radiation-induced pulmonary microvascular endothelial cells (PMECs) injury and the associated molecular mechanisms.

Materials And Methods: C57BL/6 mice were used for studies and primary human PMECs (PPMECs) were utilized as models.

The LBD Transcription Factor Confers Drought Tolerance in Transgenic Arabidopsis.

Drought stress severely impacts maize productivity, necessitating the exploration of molecular mechanisms underlying drought responses. In maize, while Class I members of the LBD family have been extensively studied for their essential functions in developmental regulation and environmental stress responses, the potential involvement of Class II LBD genes in abiotic stress tolerance mechanisms remains poorly characterized. This study characterizes , a maize Class II LBD gene, to elucidate its role in drought responses.

JAB-3312, a Potent Allosteric SHP2 Inhibitor That Enhances the Efficacy of RTK/RAS/MAPK and PD-1 Blockade Therapies.

Purpose: Recent advances have seen the development of targeted therapeutics against the receptor tyrosine kinase (RTK)/RAS/MAPK pathway, which, when aberrantly activated, drives the malignant transformation of many cancer indications. However, the efficacy of inhibitors targeting single molecules is dampened by pathway feedback activation and acquired drug resistance. We seek to evaluate the application of JAB-3312 (sitneprotafib), a potent inhibitor of SHP2, in RTK/RAS/MAPK pathway-targeted combination therapies.

[Study on Reentry Strategy and Results of Blood Donors with Single Reagent Reactivity in Wuhan Area].

Objective: To study the results, re-donation situation and characteristics of single-reagent reactive blood donors who were put into the reentry strategy in Wuhan area, explore the rationality and effectiveness of the current reentry strategy, and provide data support for the improvement of the reentry process of blood donors.

Methods: From January 2020 to December 2023, blood donors who conform the reentry criteria and voluntarily applied for returning to Wuhan Blood Center were tested and the results were analyzed. According to the reentry strategy, serological testing and nucleic acid testing were carried out in parallel, serological testing was performed by ELISA with reagents from two different manufacturers, and the primary reactive samples were tested by double-well retest, and HBV/HCV/HIV nucleic acid detection was performed by RT-PCR with an individual donor test mode.

A Novel Mouse Model of Depression: Advantages in Immune Research and Clinical Translation.

The role of neuroimmune mechanisms in major depressive disorder (MDD) has been gradually highlighted, but existing classical animal models of MDD have limitations in immune inflammation research due to physical injury, high mortality rates, and immune tolerance. This study developed a novel mouse model of depression called the post-witness social defeat stress (PWSDS) model, which combines witness stress with the social defeat paradigm. The model was evaluated based on behavior, central and peripheral immune responses, and predictive validity.

An inactivated bivalent vaccine effectively protects Carassius auratus against Aeromonas caviae and Aeromonas veronii.

Aeromonas veronii (A. veronii) and Aeromonas caviae (A. caviae) are important pathogens that affect various fish species.

Non-classic deubiquitinase USP13 inhibits bladder cancer metastasis through destabilizing cytoplasmic KDM3A.

Bladder cancer (BLCa) metastasis is a predominant cause of death for bladder cancer patients. Histone demethylase KDM3A specifically removes the repressive mono- or di-methyl marks from H3K9 and thus contributes to the activation of gene transcription. However, the underlying mechanisms of KDM3A in bladder cancer are poorly understood.

Inhibition of microRNA-139-5p by glucagon-like peptide-1 ameliorates oxidative stress-induced vascular endothelial cell damage via targeting SOD1/GCLc.

Abstract: Oxidative stress is a key driving factor for the progression of vascular disease in diabetes, and is closely related to endothelial dysfunction. The exact mechanism by which glucagon-like peptide-1 (GLP-1) directly protects vascular endothelium by reducing oxidative stress is not yet fully understood. In this study, we investigated the protective effect of GLP-1 on endothelial cells exposed to palmitic acid (PA)/high glucose-induced oxidative stress and further explored the potential mechanisms involved in microRNA-139-5p (miR-139-5p) regulation.