Priming with FLO8-deficient Candida albicans induces Th1-biased protective immunity against lethal polymicrobial sepsis.

The morphological switch between yeast and hyphae of Candida albicans is essential for its interaction with the host defense system. However, the lack of understanding of host-pathogen interactions during C. albicans infection greatly hampers the development of effective immunotherapies.

CaMac1, a Candida albicans copper ion-sensing transcription factor, promotes filamentous and invasive growth in Saccharomyces cerevisiae.

Molecular mechanisms of morphogenesis share many common components between Candida albicans and Saccharomyces cerevisiae. The Kss1-associated MAPK cascade and the cAMP/PKA pathway are two important signal transduction pathways that control morphogenesis in S. cerevisiae.

Analysis of the interaction between hPFTAIRE1 and PLZF in a yeast two-hybrid system.

hPFTAIRE1 is a Cdc2-related kinase family member. To search its substrates and regulatory proteins, hPFTAIRE1 was fused to LexA and used as a bait to screen a human brain LexA two-hybrid library. In this screening, seven hPFTAIRE1 interacting proteins, including promyelocytic leukemia zinc finger (PLZF), were obtained.

Transiently expressed short hairpin RNA targeting 126 kDa protein of tobacco mosaic virus interferes with virus infection.

RNA interference (RNAi) silences gene expression by guiding mRNA degradation in a sequence-specific fashion. Small interfering RNA (siRNA), an intermediate of the RNAi pathway, has been shown to be very effective in inhibiting virus infection in mammalian cells and cultured plant cells. Here, we report that Agrobacterium tumefaciens-mediated transient expression of short hairpin RNA (shRNA) could inhibit tobacco mosaic virus (TMV) RNA accumulation by targeting the gene encoding the replication-associated 126 kDa protein in intact plant tissue.

[Cloning and functional study of CaPPe1 in Candida albicans by using Saccharomyses cerevisiae model system].

The dimorphic transition of yeast and hyphal forms is one of most determinants in Candida albicans for its pathogenicity. This transition is regulated by several signal transduction pathways. Transcriptional factor Flo8 plays an important role in morphogenesis of Saccharomyces cerevisiae.

A viral protein suppresses siRNA-directed interference in tobacco mosaic virus infection.

Plant viruses encode suppressors of post-transcriptional gene silencing (PTGS), an adaptive defense response that limits virus replication and its spread in plants. The helper component proteinase (HC-Pro) of the potato virus A (PVA, genus Potyvirus) suppresses PTGS of silenced transgenes. Here, the effect of HC-Pro on siRNA-directed interference in the tobacco mosaic virus (TMV) was examined by using a transient Agrobacterium tumefaciens-based delivery system in intact tissues.

[Long terminal repeats of retroposons in Candida albicans].

25 Candida albicans strains have been analysed on Southern hybridization patterns with alpha, beta, gamma, kappa probes. The alpha, beta, gamma, kappa, elements can be used to distinguish the differences and relations between C. albicans strains.

[The role of Cln3 in filamentous growth and invasive growth of Saccharomyces cerevisiae].

Cln3 is one of G1 cyclins in Saccharomyces cerevisiae. In order to study the function of Cln3 in cell cycle and morphogenesis, we constructed a cln3 null mutant and analyzed its phenotype. Our results indicated that the cln3 null mutant was more sensitive to alpha pheromone, and arrested at G1 phase.

[Expression of a human ribonuclease inhibitor variant in Escherichia coli and silkworm insect cell (Bombyx mori)].

Ribonuclease inhibitor (RI) is an acidic 50 kD protein with a high content of leucine and cysteine residues. RI inhibits RNases of the pancreatic type. A variant of RI was cloned from human fetal liver cDNA library by polymerase chain reaction (PCR).

[Cloning of Candida albicans CaBEM1 and its role in filamentous growth of Saccharomyces cerevisiae].

The pathogenic fungus Candida albicans has a dimorphic transition in various environmental conditions. Many regulatory factors and several transduction pathways have been identified in controlling filamentous growth. G(1) cyclins Cln1 and Cln2 have been reported as involved in the control of morphogenesis in Saccharomyces cerevisiae.

Functions of CRK1 Gene of Candida albicans as Studied by Gene Knock-out.

In the course of screening MAPK related protein kinase genes from Candida albicans DNA library, a CRK1 (CDC2-related protein kinase) gene was identified. Deletion of the CRK1 gene significantly decreased the growth. rate of cells.

Molecular Cloning of MAPK Gene Family Using Synthetic Oligonucleotide Probe.

MAPK(mitogen activated protein kinase) is a kind of Ser/Thr protein kinase. The MAPKs play an important role in several different signal transduction pathways. The MAPKs may also have a role in morphorgenesis of Candida albicans.

KIAA0202, a human septin family member, interacting with hPFTAIRE1.

hPFTAIRE1 is a member of Cdc2-related kinase family localized in cytoplasm. To search its substrates and regulatory proteins, the hPFTAIRE1 was fused to LexA and used as bait to screen a human brain LexA two-hybrid library. In this screening, 7 hPFTAIRE1 interacting proteins, including KIAA0202, were obtained.

Molecular Cloning of MAPK Gene Family Using Degenerate PCR.

MAPK(Mitogen-activated protein kinase) pathways play an important role in morphorgenesis of Candida albicans. According to the conserved amino acid sequence of the known MAPKs, two degenerate primers attaching to subdomain VIB and IX were designed to screen novel MAPKs in C. albicans.

CEK2, a Novel MAPK from Candida albicans Complement the Mating Defect of fus3/kss1 Mutant.

A novel MAPK(mitogen-activated protein kinase) gene, CEK2(Candida albicans extracellular signal-regulated kinase 2), was isolated by screening the Candida albicans library based on oligonucleotide probe hybridization and degenerated PCR. The CEK2 gene is 1 119 bp in length, and coding for a 373 aa protein. The CEK2 shares 56% homology with CEK1 from Candida albicans, 55 % homology with FUS3 from S.

Using Kl LAC4 as a Reporter Gene in Candida albicans.

The lacZ gene which codes for beta-galactosidase from E.coli does not work in Candida albicans. In this work a reporter system with Kl LAC4 gene was contructed, coding for beta-galactosidase from Kluyveromyces lactis.

Cloning and Identification of Genes Related with Morphogenesis of Candida albicans.

A Candida albicans cDNA library was constructed and screened by differential hybridization. In hybridization using probes derived from population of yeast cells or hyphae, 67 recombinant phages exhibited more intense signal with the probe derived from hyphae than with the probe from yeast cells. One phage behaved vice versa.

Cloning and Functional Analysis of ALS Family Genes from Candida albicans.

With a 0.5 kb probe of CX2, distribution of CX2 tandem repeats was studied in different C.albicans strains.

Construction of Candida albicans Two-hybrid Library and Screening for Proteins Interacting with Crk1.

In order to study interactions among proteins involved in Candida albicans morphorgenesis, C.albicans genomic DNA was digested with Sau3AI and fused to activating domain(AD) of LEXA to construct an AD-fused C.albicans genomic DNA expression library.