m6A-modified circZNF548 regulates exosomal miR-7108-3p to activate CD3CD8 T cells and suppress NSCLC growth by JMY.

Background: Communication between cancer cells and tumor microenvironment (TME) plays a complicated role in cancer malignancy. Circular RNAs (circRNAs), known for their stability and conservation, contribute to TME remodeling in various cancers. This study aims to investigate the role of N6-methyladenosine (m6A)-modified circZNF548 in the proliferation and migration of non-small cell lung cancer (NSCLC) within the TME.

IL-12-Overexpressed Nanoparticles Suppress the Proliferation of Melanoma Through Inducing ICD and Activating DC, CD8 T, and CD4 T Cells.

Purpose: The drug resistance and low response rates of immunotherapy limit its application. This study aimed to construct a new nanoparticle (CaCO-polydopamine-polyethylenimine, CPP) to effectively deliver interleukin-12 (IL-12) and suppress cancer progress through immunotherapy.

Methods: The size distribution of CPP and its zeta potential were measured using a Malvern Zetasizer Nano-ZS90.

High-Valence Metal Doping Induced Lattice Expansion for M-FeNi LDH toward Enhanced Urea Oxidation Electrocatalytic Activities.

The precise design of low-cost, efficient, and definite electrocatalysts is the key to sustainable renewable energy. The urea oxidation reaction (UOR) offers a promising alternative to the oxygen evolution reaction for energy-saving hydrogen generation. In this study, by tuning the lattice expansion, a series of M-FeNi layered double hydroxides (M-FeNi LDHs, M: Mo, Mn, V) with excellent UOR performance are synthesized.

[Correlation of Treg and IL-15 expression in the peripheral blood of patients with oral lichen planus].

Purpose: To investigate the expression of Treg and IL-15 in the peripheral blood of patients with oral lichen planus (OLP).

Methods: The peripheral blood was obtained from 36 OLP patients and 20 healthy controls. Flow cytometry was used to evaluate the proportion of Treg cells, and the level of IL-15 was measured by ELISA.

[The up-regulated expression of LAIR-1 in tumor patients PBMC].

Aim: To investigate the expression of LAIR-1 in patients with tumors and the function of LAIR-1 in anticancer immunity.

Methods: A sandwich ELISA was employed to detect the serum sLAIR-1 from tumor patients and healthy individuals. The expression of LAIR-1 in CD4+ T cells, CD8+ T cells, NK cells and B cells isolated from the peripheral blood of cancer patients was examined by fluorescence staining and flow cytometry.

[Identification of cell lines expressing the putative ligand(s) for LAIR-1(CD305) and LAIR-2(CD306)].

Aim: To identify cell lines expressing the putative ligand(s) for LAIR-1 and LAIR-2.

Methods: CHO cell lines secreting LAIR-1-Fc or LAIR-2-Fc fusion protein were prepared and the supernatants from the CHO cell lines were collected and purified by protein A affinity chromatography column. Several cell lines were stained with the purified LAIR-1-Fc and LAIR-2-Fc fusion proteins and then analyzed by flow cytometry for detecting the expression of their putative ligand(s).

[Expression and biology identification of the human epididymis-specific gene ESC42 in E. coli].

Objective: To provide materials for the study of the function of ESC42 protein specifically expressed in the human epididymis.

Methods: The ESC42 gene was amplified from the human epididymis cDNA library by PCR and then cloned into prokaryotic expression vector pGEX-4T-1, expressed and purified by recombinant DNA techniques. The specificity of ESC42 protein was identified by Western blot and MALDI-TOF-MS.

[Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein].

Aim: To prepare and characterize mAb to Fc fragment of Ig fusion protein, and to establish sandwich ELISA for detecting Ig fusion proteins and affinity chromatography method for Ig fusion protein purification.

Methods: hBCMA-Ig was used as antigen to immune BALB/c mice. The lymphocyte hybridoma technique was used to establish hybridoma cell lines stably secreting anti-Fc mAb.

[Cloning, prokaryotic expression of LAIR and identification of their immunological reactivities].

Aim: To clone and express the leukocyte-associated immunoglobulin-like receptor (LAIR) and to identify the immune reactivity of LAIR-2 to anti-LAIR-1 specific monoclonal antibodies(mAb).

Methods: Genes encoding LAIR-1 and LAIR-2 were cloned by RT-PCR from human peripheral blood mononuclear cells(PBMC) and two leukemia cell lines Jurkat and HL-60. The extracellular region gene of LAIR1 and LAIR-2 were inserted into vector pGEX-4T-3 expressing GST fusion protein, expressed on IPTG induction and purified through glutathione-sepharose 4B column.