Biotransformation of medicarpin from homopterocarpin by Aspergillus niger and its biological characterization.

The objective of this study was to convert homopterocarpin derived from Pterocarpus macrocarpus Kurz. heartwood to medicarpin using Aspergillus niger (strain UI X-172) and assess its antioxidant, antiplasmodial, and anticancer activities in silico and in vitro. This study highlighted biotransformation of homopterocarpin to medicarpin via demethylation.

Production and characterization of extracellular liamocins produced from fungal strains of Aureobasidium spp.

Liamocins, a group of high-density glycolipids, are only produced by certain strains of the yeast-like fungi in the genus Aureobasidium. Until now, few studies have focused on the surfactant properties of liamocins produced from the highly diverse tropical strains of Aureobasidium. Therefore, the aims of this research were to screen the liamocin production from tropical strains of Aureobasidium spp.

Xylooligosaccharides produced from sugarcane leaf arabinoxylan using xylanase from NRRL 58523 and its prebiotic activity toward spp.

In an attempt to enhance the value of sugarcane leaf, xylan was extracted and used for xylooligosaccharide (XO) production via enzymatic hydrolysis using xylanase from the black yeast . The xylan was extracted from sugarcane leaf using alkali extraction according to the response surface methodology. The highest xylan yield (99.

anti-SARS-CoV-2, antiplasmodial, antioxidant, and antimicrobial activities of crude extracts and homopterocarpin from heartwood of Kurz.

Natural products play an essential role in new drug discovery. In the present study, we determined the anti-SARS-CoV-2 (severe acute respiratory syndrome-related coronavirus-2), antioxidant, antiplasmodial, and antimicrobial activities of Kurz. heartwood and structurally characterized the bioactive compounds.

Production and characterization of thermostable acidophilic β-mannanase from NRRL 58524 and its potential in mannooligosaccharide production from spent coffee ground galactomannan.

Unlabelled: The maximum yield of the crude mannanase from NRRL 58524 was 8.42 ± 0.18 U mL when cultured for 72 h in the optimized medium containing 3% (w v) defatted spent coffee grounds (SCG) and 0.

Antioxidant and UV-Blocking Properties of a Carboxymethyl Cellulose-Lignin Composite Film Produced from Oil Palm Empty Fruit Bunch.

Oil palm empty fruit bunch (EFB) pulp with the highest cellulose content of 83.42% was obtained from an optimized process of acid pretreatment (0.5% v/v HSO), alkaline extraction (15% w/w NaOH), and hydrogen peroxide bleaching (10% w/v HO), respectively.

Toxicity evaluation and biodegradation of phenanthrene by laccase from PBURU 12.

The newly isolated PBURU 12 demonstrated a high tolerance and potential for the degradation of phenanthrene. The fungal isolate was able to tolerate 100 ppm of phenanthrene with 45% relative growth. The crude laccase produced by PBURU 12 was able to degrade phenanthrene by up to 98% within 24 h.

Hepatoprotective effect of crude polysaccharides extracted from against carbon tetrachloride-induced liver injury in mice.

Background And Aim: Natural products are currently widely used as alternative treatments for liver disease. The study aimed to determine the hepatoprotective effect of crude polysaccharides extracted from against liver injury induced by carbon tetrachloride (CCl).

Materials And Methods: Twenty-four male BALB/C mice were randomly divided into six groups.

Production of cutinase from and its application for hydrophilicity improvement of polyethylene terephthalate fabric.

Among 23 isolates of cutinase-producing fungi from Thailand, one strain of PBURU-T5 exhibited the greatest cutinase activity (3.36 ± 0.12 U ml) against -nitrophenyl butyrate.

Purification and Characterization of Cellulase from Obligate Halophilic Aspergillus flavus (TISTR 3637) and Its Prospects for Bioethanol Production.

A cellulase from the extreme obligate halophilic fungus, Aspergillus flavus, isolated from a man-made solar saltern in Phetchaburi, Thailand, was purified by ammonium sulfate precipitation and using Sephadex G-100 gel filtration column chromatography. The cellulase was found to be approximately 55 kDa by SDS-PAGE. Using CMC as a substrate, the specific activity of the cellulase was 62.

Fusigen Reduces Intracellular Reactive Oxygen Species and Nitric Oxide Levels.

Background/aim: Oxidative stress caused by the production of excessive cellular reactive oxygen species (ROS) and high levels of nitric oxide contribute to several human pathologies. This study aimed to examine the anti-oxidant effects of fusigen, a compound produced from Aureobasidium melanogenum.

Materials And Methods: Extracts of A.

Enzymatic hydrolysis of tropical weed xylans using xylanase from PBUAP46 for xylooligosaccharide production.

The maximum yield of xylanase from PBUAP46 was 5.19 ± 0.08 U ml when cultured in a production medium containing 3.

The current status of Aureobasidium pullulans in biotechnology.

Different strains of the saprophytic yeast-like fungus Aureobasidium pullulans (Ascomycota: Dothideales) exhibit different biochemical characteristics, while their ubiquitous occurrence across diverse habitats and environmental conditions makes them an easily accessible source for biotechnological exploitation. They are useful in agricultural and industrial applications. Their antagonistic activities against postharvest pathogens make them suitable bioagents for the postharvest preservation of fruits and vegetables, while they possess antimicrobial activities against bacteria and fungi.

Enzymatic Hydrolysis of Black Liquor Xylan by a Novel Xylose-Tolerant, Thermostable β-Xylosidase from a Tropical Strain of Aureobasidium pullulans CBS 135684.

From three cell-associated β-xylosidases produced by Aureobasidium pullulans CBS 135684, the principal enzyme was enriched to apparent homogeneity and found to be active at high temperatures (60-70 °C) over a pH range of 5-9 with a specific activity of 163.3 units (U) mg. The enzyme was thermostable, retaining over 80% of its initial activity after a 12-h incubation at 60 °C, with half-lives of 38, 22, and 10 h at 60, 65, and 70 °C, respectively.

Purification and Characterization of a Polyextremophilic α -Amylase from an Obligate Halophilic Aspergillus penicillioides Isolate and Its Potential for Souse with Detergents.

An extracellular α-amylase from the obligate halophilic Aspergillus penicillioides TISTR3639 strain was produced and enriched to apparent homogeneity by ammonium sulfate precipitation and Sephadex G100 gel filtration column chromatography. The mass of the purified amylase was estimated to be 42 kDa by SDS-PAGE. With soluble starch as the substrate it had a specific activity of 118.

Purification, characterization, and potential of saline waste water remediation of a polyextremophilic α-amylase from an obligate halophilic Aspergillus gracilis.

An obligate halophilic Aspergillus gracilis which was isolated from a hypersaline man-made saltern from Thailand was screened for its potential of producing extracellular α -amylase in the previous studies. In this study the α -amylase was extracted and purified by the help of column chromatography using Sephadex G-100 column. Presence of amylase was verified by SDS-PAGE analysis, showing a single band of approximately 35 kDa.

Effect of polyols on thermostability of xylanase from a tropical isolate of Aureobasidium pullulans and its application in prebleaching of rice straw pulp.

In an attempt to find a thermostable xylanase enzyme for potential application in the pretreatment prior to H2O2 bleaching of paper pulp for industry, an extracellular xylanase from Aureobasidium pullulans CBS 135684 was purified 17.3-fold to apparent homogeneity with a recovery yield of 13.7%.

Effects of sugar and amino acid supplementation on Aureobasidium pullulans NRRL 58536 antifungal activity against four Aspergillus species.

Cultured cell extracts from ten tropical strains of Aureobasidium pullulans were screened for antifungal activity against four pathogenic Aspergillus species (Aspergillus flavus, Aspergillus niger, Aspergillus fumigatus, and Aspergillus terreus) using the well diffusion and conidial germination inhibition assays. The crude cell extract from A. pullulans NRRL 58536 resulted in the greatest fungicidal activity against all four Aspergillus species and so was selected for further investigation into enhancing the production of antifungal activity through optimization of the culture medium, carbon source (sucrose and glucose) and amino acid (phenylalanine, proline, and leucine) supplementation.

Heavy oils produced by Aureobasidium pullulans.

From a survey of more than 50 diverse strains of Aureobasidium pullulans, 21 produced extracellular heavy oils. Most oil producers fell into phylogenetic clades 8, 9, and 11. Oil colors ranged from bright yellow to malachite.

α-Amylase activity during pullulan production and α-amylase gene analyses of Aureobasidium pullulans.

Aureobasidium pullulans is the source of commercially produced pullulan, a high molecular weight polysaccharide that is used in the manufacture of edible films. It has been proposed that α-amylase decreases the molecular weight of pullulan in late cultures. Based on a recent phylogenetic analysis, five representative strains were chosen to study the relationship between α-amylase and pullulan production.

Multilocus phylogenetic analyses, pullulan production and xylanase activity of tropical isolates of Aureobasidium pullulans.

Aureobasidium pullulans is the source of the commercially valuable polysaccharide pullulan and the enzyme xylanase. Isolates are typically off-white to pale pink or black on solid media, while some tropical isolates have been described as 'color variants' with bright pigments of red, yellow or purple. We sequenced 5 loci (internal transcribed spacer, intergenic spacer 1, translation elongation factor-1 alpha, beta tubulin, and RNA polymerase II) from 45 new isolates from Thailand.

Screening of tropical fungi producing polyethylene terephthalate-hydrolyzing enzyme for fabric modification.

Microfungi were selectively isolated for production of polyethylene terephthalate (PET) fiber-degrading enzymes potentially to be used to modify the surface of polyester fabric. A range of fungi were isolated from plant surfaces and soil samples using a polycaprolactone (PCL) plate-clearing assay technique, and screened for cutinolytic esterase (cutinase) activity. Twenty-two of 115 isolates showed clearing indicating the production of cutinase.