Ratiometric absorbance and fluorescence dual model immunoassay to detect ochratoxin a based on ZIF-8@uranine and p-nitrophenol.

Ochratoxin A (OTA) as a mycotoxin has strong carcinogenicity and can affect human health. Thus, it is necessary to establish an accurate method to detect OTA. Herein, a ratiometric absorbance and fluorescence dual model immunoassay based on ZIF-8@uranine (ZIF-8@UR) and p-nitrophenol (PNP) was developed.

Fluorescence and colorimetric dual-mode detection of tetracycline antibiotics based on Eu and the peroxidase mimetic activity of a FeO@MoS nanocomposite.

 A sensitive fluorescence and colorimetric dual-mode detection method based on Eu and FeO@MoS nanocomposites was constructed. In this technique, tetracycline antibiotics (TCs) chelated with Eu to form a Eu-TCs complex which emits red fluorescence at 620 nm through the antenna effect. At the same time, Eu-TCs complex enhances the peroxidase-like activity of FeO@MoS to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) that can produce a colorimetric signal.

Atomically Dispersed Cobalt within a Bilayer of C.

Individual cobalt atoms trapped within a bilayer of C have been produced by the deposition of Co atoms onto a C double layer supported on graphite. High-resolution scanning tunneling microscopy (STM) reveals two stable states of a single cobalt atom coordinated with three C molecules, which is denoted as (C)Co. The diffusion of the cobalt atoms within the C layers is highly limited, resulting in the effective capture of single cobalt atoms in the interstitial sites of the close-packed C layers.

Single-Model Self-Recovering Fringe Projection Profilometry Absolute Phase Recovery Method Based on Deep Learning.

A drawback of fringe projection profilometry (FPP) is that it is still a challenge to perform efficient and accurate high-resolution absolute phase recovery with only a single measurement. This paper proposes a single-model self-recovering fringe projection absolute phase recovery method based on deep learning. The built Fringe Prediction Self-Recovering network converts a single fringe image acquired by a camera into four single mode self-recovering fringe images.

Triple-readout immunoassay based on copper ion trigger for the detection of ochratoxin A.

Background: Ochratoxin A (OTA) is a widespread mycotoxin with nephrotoxic, neurotoxic and immunotoxic properties. It exhibits elevated thermal stability and a prolonged half-life and is resistant to removal. Long-term exposure to OTA increases the risk of carcinogenesis and influences human health.

Dual-mode detection of Ochratoxin A based on silver nanocluster and phosphate.

Ochratoxin A (OTA) is widely present in the environment and has great harm to human safety. Therefore, a simple and sensitive method for OTA detection is urgently needed. Herein, a fluorescence and colorimetric dual-mode immunoassay based on glutathione silver nanocluster (GSH-AgNCs) and phosphate colorimetry was established.

Ratiometric absorbance and fluorescence dual model immunoassay for detecting ochratoxin a based on porphyrin metalation.

In this work, a porphyrin metalation-based ratiometric absorbance and fluorescence dual model immunoassay was proposed to detect ochratoxin A (OTA). 5,10,15,20-tetrakis(1-methyl-4-pyridinio) porphyrin (TMPyP) was pink and had a strong fluorescence, upon coordination with Hg(II), its fluorescence was quenched and the color became green. The alkaline phosphatase can catalyze the dephosphorylation of ascorbic acid 2-phosphate to produce ascorbic acid, which can reduce the coordinated Hg(II) to Hg(0) and then dissociated from TMPyP, its fluorescence was recovered.

Fine-grained recognition of bitter gourd maturity based on Improved YOLOv5-seg model.

Bitter gourd, being perishable, requires timely harvesting. Delayed harvesting can result in a substantial reduction in fruit quality. while premature harvesting leads to underdeveloped fruit and decreased yields, the continuous flowering pattern in bitter gourd underscores the significance of accurately assessing fruit growth and ensuring timely harvesting for subsequent fruit setting and development.

Ratiometric fluorescence and absorbance dual-model immunoassay based on 2,3-diaminophenazine and carbon dots for detecting Aflatoxin B1.

In this work, a dual-model immunoassay for detecting Aflatoxin B1 (AFB1) was developed based on 2,3-diaminophenazine (DAP) and carbon dots (CDs). Under the catalysis of horseradish peroxidase (HRP), the o-phthalylenediamine (OPD) was oxidized to DAP which had a yellow color and intense fluorescence. The color changes form colorless to yellow was used to design absorbance model immunoassay.

Ratiometric fluorescence immunoassay based on silver nanoclusters and calcein-Ce for detecting ochratoxin A.

Ochratoxin A (OTA), a dangerous mycotoxin, is found in many crops. It is essential to create sensitive OTA detection techniques to ensure food safety. Based on the principle of p-nitrophenol (PNP) quenched the fluorescence of bovine serum albumin silver nanocluster (BSA-AgNCs) through an internal filtering effect, and phosphate activated fluorescence of calcein-Ce system, a ratiometric fluorescence immunoassay for OTA detection was developed.

Alkaline phosphatase triggered gold nanoclusters turn-on fluorescence immunoassay for detection of Ochratoxin A.

Ochratoxin A (OTA) is a highly toxic mycotoxin which can cause a variety of diseases. Sensitive detection of OTA is significant for food safety. Herein, a feasible and sensitive immunoassay was established for OTA detection by alkaline phosphatase (ALP) triggered gold nanoclusters (AuNCs) turn-on fluorescence.

Gold nanoparticle-mediated fluorescence immunoassay for rapid and sensitive detection of Ochratoxin A.

In this work, a fluorescence immunoassay based on horseradish peroxidase-labeled IgG (HRP-IgG)-modified gold nanoparticle (AuNP@HRP-IgG) probe was established for detection of ochratoxin A (OTA). Through the catalysis of HRP, the dopamine (DA) and 1,5-dihydroxynaphthalene (DHA) can rapidly generate azamonardine fluorescence compound (AFC) with intense yellow fluorescence. Large amounts of AFC can be formed within 4 min, which led to fluorescence enhancement at 545 nm.

DSIL-DDI: A Domain-Invariant Substructure Interaction Learning for Generalizable Drug-Drug Interaction Prediction.

Drug-drug interactions (DDIs) trigger unexpected pharmacological effects in vivo, often with unknown causal mechanisms. Deep learning methods have been developed to better understand DDI. However, learning domain-invariant representations for DDI remains a challenge.

Construction Sheets Made of High-Performance Flame-Retardant Nonwoven Fabrics and Combustion-Resistant Polyurethane Foam: Preparation Process and Property Evaluations.

In this study, nonwoven fabrics, rigid polyurethane foam (RPUF), Basalt woven fabrics, and an aluminum foil film mold are used to produce multi-functional composite sheets with flame-retardant, sound-absorbing, and electromagnetic-shielding functions. The nonwoven layer is composed of Nomex fibers, flame-retardant PET fibers, and low-melting-point (LMPET) fibers via the needle rolling process. The optimal Nomex fiber/flame-retardant PET fiber/LMPET fiber (N/F/L) nonwoven fabrics are then combined with rigid polyurethane (PU) foam, Basalt woven fabric, and an aluminum foil film mold, thereby producing nonwoven/rigid polyurethane foam/Basalt woven fabric composite sheets that are wrapped in the aluminized foil film.

Characterization of highly polymorphic microsatellite markers for the chinese monal (Lophophorus lhuysii, Galliformes) using Illumina MiSeq sequencing.

Background: The Chinese monal (Lophophorus lhuysii, Galliformes) is a vulnerable and endemic bird from southwestern China. To better protect this species and increase its population size, genetic markers are urgently needed for investigation and conservation of both wild and captive populations.

Methods And Results: By using next-generation sequencing, we developed and characterized markers for seven microsatellite loci of the Chinese monal.

Kinetic Controlled Chirality Transfer and Induction in 2D Hydrogen-Bonding Assemblies of Glycylglycine on Au(111).

Chirality transfer is of vital importance that dominates the structure and functionality of biological systems and living matters. External physical stimulations, e.g.

MnO nanosheets-triggered oxVB fluorescence immunoassay for detection zearalenone.

In this study, an alkaline phosphatase (ALP)-mediated fluorescence immunoassay for detecting zearalenone (ZEN) was established based on the oxVB fluorescence signal modulated by MnO nanosheets (MnO NS). As the ALP-antibody content increased, more 2-phosphoascorbic acid (AAP) was hydrolyzed to ascorbic acid (AA) which destroyed the MnO NS rapidly. In the lack of MnO NS, VB cannot be oxidized to oxVB for emitting fluorescence.

Cerium ions triggered dual-readout immunoassay based on aggregation induced emission effect and 3,3',5,5'-tetramethylbenzidine for fluorescent and colorimetric detection of ochratoxin A.

Ochratoxin A (OTA) is one of the most prevalent and toxic mycotoxins. Ultrasensitive and convenient detection of OTA is urgent demanded for public health. In this work, a dual-readout immunoassay was established for the detection of OTA based on Ce oxidizing 3,3',5,5'-tetramethylbenzidine (TMB) and Ce inducing aggregation induced emission (AIE) of Au nanoclusters (AuNCs).

Automatic Segmentation of Lumbar Spine MRI Images Based on Improved Attention U-Net.

Lumbar spine segmentation is important to help doctors diagnose lumbar disc herniation (LDH) and patients' rehabilitation treatment. In order to accurately segment the lumbar spine, a lumbar spine image segmentation algorithm based on improved Attention U-Net is proposed. The algorithm is based on Attention U-Net, the attention module based on multilevel feature map fusion is adopted, two residual modules are introduced instead of the original convolution blocks.

Fluorescence and colorimetric dual-mode immunoassay based on G-quadruplex/N-methylmesoporphyrin IX and p-nitrophenol for detection of zearalenone.

In this work, a fluorescence and colorimetric dual-mode immunoassay for detecting zearalenone (ZEN) was established. This platform relied on the dephosphorylation of p-nitrophenyl phosphate (PNPP) by alkaline phosphatase (ALP) to produce yellow p-nitrophenol (PNP). And the internal filtration effect between G-quadruplex/N-methylmesoporphyrin IX (G4/NMM) and PNP led to fluorescence quenching of G4/NMM.

Arterial spin labeling of nasopharyngeal carcinoma shows early therapy response.

Objective: This study aimed to determine the value of arterial spin labeling (ASL) perfusion imaging in assessing the early efficacy of chemoradiotherapy for nasopharyngeal carcinoma (NPC).

Methods: Fifty-five patients with locoregionally advanced NPC underwent conventional 3.0-T magnetic resonance imaging (MRI) and ASL before and after chemoradiotherapy (prescribed dose reached 40 Gy).

Fluorescence and absorbance dual-mode immunoassay for detecting Ochratoxin A.

In this work, a simple dual-mode immunoassay for detecting Ochratoxin A (OTA) was developed by mixing G-quadruplex/N-methylmesoporphyrin IX (G4/NMM) and 3,3',5,5'-tetramethylbenzidine (TMB). The fluorescence of G4/NMM can be quenched by oxidized TMB (oxTMB) because the absorbance of oxTMB overlapped with the fluorescence emission of G4/NMM. In the absence of OTA, large amounts of oxTMB were formed with blue color and the fluorescence of G4/NMM was quenched.

Fluorescence determination of glyphosate based on a DNA-templated copper nanoparticle biosensor.

A rapid and convenient fluorescence glyphosate (GLYP) biosensor was developed based on DNA-templated copper nanoparticles (DNA-CuNPs). In the absence of GLYP, the DNA-CuNPs were formed through the reduction of Cu by vitamin C (Vc). The DNA-CuNPs emitted intense fluorescence at 615 nm when being excited at 340 nm.

A novel fluorescence method for detection of phosphate anions based on porphyrin metalation.

In this work, a novel fluorescence method for the detection of phosphate anions (PO) was developed based on porphyrin metalation. Through catalysis by G-quadruplex (G4), Cu could insert into the porphyrins to quench their fluorescence. G4 simultaneously improved the fluorescence of the porphyrins but not that of Cu-porphyrin.