Effects of directly printed aligner eluent on the viability and transcriptome of gingival fibroblasts.

Objectives: This study aimed to evaluate the cytotoxicity of directly-printed aligners (DPAs) by simulating sequential elution under oral conditions. The research also sought to identify leachable compounds and their potential effects on human gingival fibroblasts (HGFs).

Methods: DPAs were fabricated using a 3D printing system and cut into small pieces for immersion in artificial saliva.

Impact of romosozumab treatment for 24 months after switching from denosumab: three case reports.

Prior denosumab use in osteoporosis patients diminishes the BMD gains from a subsequent 12-mo romosozumab regimen. However, few studies have explored BMD gains after 24 mo of romosozumab therapy. BMD at the LS-BMD, FN-BMD, and TH-BMD and P1NP and C-terminal telopeptide of type I collagen (CTX) levels were measured after 24 mo of romosozumab therapy in 3 osteoporosis cases with prior denosumab use.

Novel quinazoline-triazole-based -hydroxybenzamides/-hydroxypropenamides as HDAC inhibitors: design, synthesis, biological evaluation, and docking studies.

Herein, two novel series of -hydroxybenzamides and -hydroxypropenamides incorporating 4-oxoquinazoline and 1,2,3-triazole scaffolds were rationally designed, synthesized, and evaluated for their Histone deacetylase (HDAC) inhibitory and anticancer activities. The synthesized hydroxamic acids were evaluated for HDAC inhibitory activity using nuclear extracts from HeLa cells. -hydroxybenzamide derivatives (7a-i) exhibited stronger HDAC inhibition than their -hydroxypropenamide counterparts (11a-i).

Association with Elevated Somatic Cell Counts and Characterization of Aerococcus viridans Isolates from Bovine Mastitis Milk in South Korea.

Aerococcus viridans, an emerging pathogen, is responsible for the recent increase in cases of bovine mastitis. However, its specific effects on mastitis remain largely unexplored. In this study, we examined the correlation between A.

Risk Stratification of Thyroid Nodules Diagnosed as Follicular Neoplasm on Core Needle Biopsy.

Backgruound: This study assessed risk stratification and diagnostic performance for malignancy in thyroid nodules diagnosed as follicular neoplasm (FN) based on core needle biopsy (CNB) subcategories.

Methods: A total of 313 consecutive nodules (>1 cm) diagnosed as FN on CNB with corresponding surgical histology were included. FN subcategories were classified retrospectively for nodules diagnosed before 2022 (retrospective dataset) and prospectively for nodules diagnosed since 2022 (prospective dataset).

Factors Associated With Compliance and Persistence With Pharmacotherapy in Patients With Osteoporosis: A Nationwide Cohort Study in Korea.

Background: Despite the necessity of long-term management for fracture risk reduction, adherence to osteoporosis pharmacotherapy remains poor. We investigated the factors influencing adherence to pharmacotherapy among Korean patients with osteoporosis, with a particular focus on treatment with bisphosphonates (BPs).

Methods: Data from 725,313 osteoporosis patients newly prescribed BPs or selective estrogen receptor modulators (SERMs) between 2012 and 2014, obtained from the Korean National Health Insurance Service, were analyzed.

Twenty-Year Trends in Osteoporosis Treatment and Post-Fracture Care in South Korea: A Nationwide Study.

Background: Osteoporosis is a significant public health issue in aging populations. Despite advances in pharmacotherapy, underdiagnosis and undertreatment remain prevalent even in patients with recent fractures. This study examined 20-year trends (2002- 2022) in anti-osteoporotic medication (AOM) usage in South Korea.

Whole-genome sequencing-based characterization of Listeria monocytogenes isolated from cattle and pig slaughterhouses.

Listeria monocytogenes is a foodborne pathogen that causes human listeriosis and may be transmitted to humans via the food chain, beginning at slaughter and extending through food production and consumption. In this study, we performed whole-genome sequencing (WGS) analysis to determine the genetic characteristics of L. monocytogenes from the carcasses and environments of cattle and pig slaughterhouses in Korea.

Epidemiology of osteoporotic ankle fractures in South Korea: a nationwide retrospective cohort study (2006-2022).

Purpose: Ankle fractures, ranking as one of the very common osteoporotic fractures, pose a substantial socioeconomic burden. We aimed to investigate the incidence of elderly ankle fractures, refracture risks, and mortality rates in South Korea.

Methods: Utilizing the Korean National Health Insurance Service (NHIS) registry from January 2006 to December 2022, individuals over 50 years with ankle fractures were identified.

Multi-label material and human risk factors recognition model for construction site safety management.

Introduction: Construction sites are prone to numerous safety risk factors, but safety managers have difficulty managing these risk factors for practical reasons. Moreover, manually identifying multiple risk factors visually is challenging. Therefore, this study aims to propose a deep learning model-based multi-label risk factor recognition (MRFR) framework that automatically recognizes multiple potential material and human risk factors at construction sites.

Ultrasonographic predictors of acute gangrenous cholecystitis in patients treated with laparoscopic cholecystectomy: a single center retrospective study.

Background: Acute gangrenous cholecystitis, a severe complication of cholecystitis, carries a high risk of morbidity and mortality. Despite its severity, preoperative diagnosis is challenging, often only confirmed during surgery. Therefore, we analyzed various factors that could predict acute gangrenous cholecystitis in patients undergoing cholecystectomy.

Evaluation of acute, repeated dose 28-day and 13-week oral toxicity and genotoxicity of a standardized fraction (HemoHIM) from .

HemoHIM is a functional food ingredient comprising a triple herbal combination of extracts from Nakai, Makino, and Pallas. It was developed to aid the recovery of impaired immune function. Although it is widely used to treat various immune disorders in Korea, its potential toxicity has not been extensively investigated.

Near-Normalized Maxillomandibular Relationship and Upper Airway in Infants with Robin Sequence Treated with Stanford Orthodontic Airway Plate: Computed Tomography Study.

Objective: To assess craniofacial and upper airway growth in infants with Robin sequence (RS) during the 1 year of life when their severe upper airway obstruction was treated non-surgically with the Stanford orthodontic airway plate treatment program (SOAP).

Design: Retrospective longitudinal cohort study comparing SOAP-treated infants with RS (treatment group) with age-matched healthy controls (HC) using computed tomography (CT).

Setting: Single tertiary referral hospital.

Characteristics of methicillin-resistant isolates from bovine mastitis milk in South Korea: molecular characteristics, biofilm, virulence, and antimicrobial resistance.

Unlabelled: Methicillin-resistant (MRSA) is a drug-resistant pathogen causing subclinical and clinical bovine mastitis. This study examined the molecular properties, biofilm formation, virulence genes, and antimicrobial susceptibility of MRSA isolates from mastitis-infected dairy cow milk in South Korea. Whole-genome sequencing of an ST22-SCC IV MRSA strain positive for Panton-Valentine leukocidin (PVL) and toxic shock syndrome toxin-1 (TSST-1) was also performed.

Prevalence of pathogens and antimicrobial resistance of isolated Staphylococcus spp. in bovine mastitis milk in South Korea, 2018-2022.

Staphylococcus spp. are one of the most predominant isolates in milk samples of dairy cows with mastitis worldwide. The aims of this study were to investigate the prevalence of bacterial pathogens in bovine mastitis milk samples in South Korea and the antimicrobial resistance profiles of staphylococcal isolates.

Impact of antiresorptive agents on mortality risk in postmenopausal women with osteoporosis: insights from a nationwide cohort study.

Importance: Osteoporosis-related fractures are associated with increased mortality risk among postmenopausal women, yet the impact of antiosteoporotic medications on mortality is not fully understood.

Objective: This study evaluates the effect of antiresorptive agents (ARs) on mortality risk in postmenopausal women with osteoporosis.

Design: This is a nationwide cohort study using data from the National Screening Program for Transitional Ages (2008-2017).

Preoperative ultrasonography parathyroid gland mapping can improve identification of normal parathyroid gland during thyroidectomy: A propensity score-matched case-control study.

Background: Accurate intraoperative identification of normal parathyroid glands (PTGs) is vital to avoid hypocalcemia post total thyroidectomy. Although ultrasonography (US) has been shown to identify normal PTGs, the significance of preoperative US PTG mapping in this context is not well studied. This study evaluated the impact of preoperative US PTG mapping on intraoperative identification of normal PTGs during total thyroidectomy.

TSpred: a robust prediction framework for TCR-epitope interactions using paired chain TCR sequence data.

Motivation: Prediction of T-cell receptor (TCR)-epitope interactions is important for many applications in biomedical research, such as cancer immunotherapy and vaccine design. The prediction of TCR-epitope interactions remains challenging especially for novel epitopes, due to the scarcity of available data.

Results: We propose TSpred, a new deep learning approach for the pan-specific prediction of TCR binding specificity based on paired chain TCR data.

Vitamin D Attenuates Fibrotic Properties of Fibrous Dysplasia-Derived Cells for the Transit towards Osteocytic Phenotype.

Fibrous dysplasia (FD) poses a therapeutic challenge due to the dysregulated extracellular matrix (ECM) accumulation within affected bone tissues. In this study, we investigate the therapeutic potential of 1,25-dihydroxyvitamin D3 (1,25(OH)D) in managing FD by examining its effects on FD-derived cells in vitro. Our findings demonstrate that 1,25(OH)D treatment attenuates the pro-fibrotic phenotype of FD-derived cells by suppressing the expression of key pro-fibrotic markers and inhibiting cell proliferation and migration.

Patient-Derived Organoids Recapitulate Pathological Intrinsic and Phenotypic Features of Fibrous Dysplasia.

Fibrous dysplasia (FD) is a rare bone disorder characterized by the replacement of normal bone with benign fibro-osseous tissue. Developments in our understanding of the pathophysiology and treatment options are impeded by the lack of suitable research models. In this study, we developed an in vitro organotypic model capable of recapitulating key intrinsic and phenotypic properties of FD.

Laboratory investigation of causes of bovine abortion and stillbirth in the Republic of Korea, 2014-2020.

Bovine abortion is a critical problem in the cattle industry. Identifying causes of abortion is key to establishing appropriate herd management and prevention strategies. We used pathology examinations, detection of etiologic agents, and serology to determine the cause of bovine abortions in Korea.