Effects of directly printed aligner eluent on the viability and transcriptome of gingival fibroblasts.

Objectives: This study aimed to evaluate the cytotoxicity of directly-printed aligners (DPAs) by simulating sequential elution under oral conditions. The research also sought to identify leachable compounds and their potential effects on human gingival fibroblasts (HGFs).

Methods: DPAs were fabricated using a 3D printing system and cut into small pieces for immersion in artificial saliva.

Single-cell RNA sequencing using split-pool barcoding reveals transcriptional heterogeneity in with implications for periodontal pathogenesis.

Background: is a keystone pathogen in periodontitis, associated with dysbiosis and chronic inflammation. While its virulence mechanisms are well characterized, its transcriptional heterogeneity at the single-cell level remains unexplored.

Materials And Methods: We applied split-pool barcoding-based single-cell RNA sequencing to profile gene expression in 1,942 individual W83 cells cultured under anaerobic conditions.

Associations between smoking, disease, and Porphyromonas gingivalis fimA genotype distribution: Insights from Korean periodontitis patients.

Objective: To investigate the distribution of Porphyromonas gingivalis fimA genotypes in relation to smoking and periodontitis severity in a Korean adult population, and to assess genotype-specific associations with host and environmental factors.

Design: Subgingival biofilm samples were obtained from 283 adults, comprising 102 non-smoking healthy individuals, 98 non-smoking periodontitis patients, and 83 smoking periodontitis patients. Bacterial DNA was extracted and analyzed using fimA-specific PCR to detect P.

Draft genome sequence of isolated from subgingival biofilm.

is generally considered beneficial for vaginal and digestive health. However, strains isolated from the oral cavity, especially from periodontitis lesions, have not been thoroughly studied. Here, we report the draft genome sequence of strain KHUD_007 isolated from the subgingival biofilm of a Korean patient with periodontitis.

Effects of Sodium Tripolyphosphate on Oral Commensal and Pathogenic Bacteria.

Polyphosphate (polyP) is a food additive with antimicrobial activity. Here we evaluated the effects of sodium tripolyphosphate (polyP3, NaPO) on four major oral bacterial species, in both single- and mixed-culture. PolyP3 inhibited three opportunistic pathogenic species: , and .

A Polysaccharide-Based Antibacterial Coating with Improved Durability for Clear Overlay Appliances.

Clear overlay appliances (COAs) are widely used in orthodontic fields because they offer many advantages, such as cost-effectiveness, good formability, and good optical characteristics. However, it is necessary to frequently replace COAs because the thermoplastic polymers that are used to fabricate COAs have poor abrasion resistance and have a tendency to induce bacterial accumulation. Here, we have developed polysaccharide-based antibacterial multilayer films with enhanced durability, intended for COA applications.