Impact of smoking and age on long-term recurrence after laparoscopic primary closure for duodenal ulcer perforation: a 5-year observational study.

Purpose: This study investigates risk factors for recurrence in patients who underwent laparoscopic primary closure (PC) for pyloroduodenal ulcer perforation (PUP).

Methods: We retrospectively analyzed data from patients who underwent laparoscopic PC with or without highly selective vagotomy (HSV) for PUP at a tertiary hospital from 2010 to 2019. Demographics, surgical outcomes, proton pump inhibitor (PPI) use, status, and endoscopic findings were reviewed.

Stressomic: A wearable microfluidic biosensor for dynamic profiling of multiple stress hormones in sweat.

Managing stress is essential for mental and physical health, yet current methods rely on subjective self-assessments or indirect physiological measurements, often lacking accuracy. Existing wearable sensors primarily target a single stress hormone, cortisol, using single-point measurements that fail to capture real-time changes and distinguish between acute and chronic stress. To address this, we present Stressomic, a wearable multiplexed microfluidic biosensor for noninvasive monitoring of cortisol, epinephrine, and norepinephrine in sweat.

Preoperative Prediction of Microvascular Invasion in Intrahepatic Cholangiocarcinoma and Its Prognostic Implications: A Multicenter Study.

Introduction: Previous studies on preoperative predictors of microvascular invasion (MVI) in intrahepatic cholangiocarcinoma (ICCA) have presented inconsistent results. This study aimed to identify preoperative clinical and magnetic resonance imaging (MRI) factors that can predict MVI in ICCA and to evaluate their prognostic utility using a multicenter cohort.

Methods: A multicenter cohort of 446 patients who underwent preoperative MRI and surgical resection for ICCA at six tertiary referral institutions between 2009 and 2018 was analyzed for clinical, pathologic, and MR imaging characteristics.

Clinical Practice Guidelines for Diagnosis and Non-Surgical Treatment of Primary Frozen Shoulder.

Objective: Primary frozen shoulder causes significant pain and progressively restricts shoulder movements. Diagnosis is primarily clinically based on patient history and physical examination. Management is mainly non-invasive owing to its self-limiting clinical course.

All-printed chip-less wearable neuromorphic system for multimodal physicochemical health monitoring.

Recent advancements in wearable sensor technologies have enabled real-time monitoring of physiological and biochemical signals, opening new opportunities for personalized healthcare applications. However, conventional wearable devices often depend on rigid electronics components for signal transduction, processing, and wireless communications, leading to compromised signal quality due to the mechanical mismatches with the soft, flexible nature of human skin. Additionally, current computing technologies face substantial challenges in efficiently processing these vast datasets, with limitations in scalability, high power consumption, and a heavy reliance on external internet resources, which also poses security risks.

LSPR-Driven Dual-Mode Urea Sensor for Milk Adulteration Detection Using Gold Nanobipyramids and Upconversion Nanoparticles.

Ensuring food quality and safety is crucial, as the consumption of contaminated or substandard food poses serious risks to public health and imposes a substantial economic burden. Among various food safety concerns, milk adulteration with urea has emerged as a significant issue. This study presents a highly sensitive dual-mode colorimetric and fluorometric urea sensor for milk adulteration detection, utilizing enzymatic hydrolysis with Fenton-mediated plasmonic etching.

Validation of prognostic models for predicting postsurgical outcomes in intrahepatic cholangiocarcinoma patients using a multicenter cohort.

Background: Few studies to date have externally validated and directly compared conventional prognostic models that predict postsurgical outcomes in patients with intrahepatic cholangiocarcinoma (ICCA). We aimed to validate the performance of prognostic models predicting postsurgical outcomes in a multicenter cohort of patients with ICCA.

Materials And Methods: Consecutive patients with ICCA who underwent curative-intent hepatic resection for ICCA at six tertiary referral institutions between January 2009 and December 2016 were retrospectively analyzed.

The Melanophilin knockout chicken, as a new alopecia animal model.

Avian alopecia, marked by feather loss, is a significant health concern impacting various bird species in both wild and captive environments. This condition arises from multiple factors, including nutritional deficiencies, hormonal imbalances, infectious diseases, environmental stressors, and genetic predispositions. Natural mutations in the Melanophilin (Mlph) gene have been linked to color-diluted alopecia in dogs, while suppression of Rab27a, a key interactor of MLPH, disrupts hair cycle regulation and impairs hair growth in mice.

MicroRNA-18b regulates cell cycle progression in papillary thyroid carcinoma by targeting : an experimental study.

Purpose: Papillary thyroid carcinoma (PTC) is the most prevalent endocrine malignancy, and the global incidence has been steadily increasing over the years. Although PTC generally exhibits favorable prognosis, a subset of patients experiences aggressive progression and increased mortality. Current prognostic approaches, reliant on clinic-pathological factors, have limitations, underscoring the need for innovative biomarkers.

Risk Factor Analysis of Simple Drainage Followed by Instrumented Fusion in Patients with Pyogenic Spinal Infection.

Objective: The first-line treatment for pyogenic spondylitis is conservative management with immobilization and systemic antibiotic therapy. However, in cases where conservative treatment fails or neurological deficits and spinal instability are present, surgical intervention is required. Surgical treatment helps manage spondylitis by decompressing neural structures through abscess drainage and maintaining spinal stability.

Preoperative scoring system for predicting microvascular invasion in intrahepatic cholangiocarcinoma using gadoxetate-enhanced MRI.

Objectives: To develop a preoperative risk scoring system to predict microvascular invasion (MVI) in intrahepatic cholangiocarcinoma (ICCA) by incorporating clinical and gadoxetate-enhanced MRI features.

Materials And Methods: We retrospectively enrolled 197 consecutive patients with ICCA who underwent preoperative gadoxetate-enhanced MRI and curative surgical resection between 2009 and 2016. The patients were randomly divided into a development set (n = 139) and a validation set (n = 58).

pH-Inhibited Fenton etching of gold nanobipyramids: a multicolor approach for enhanced urea detection.

This study presents an innovative urea detection method utilizing pH-controlled Fenton etching of gold nanobipyramids (AuNBPs), offering a multicolor visual response. By leveraging the urease-catalyzed hydrolysis of urea, which releases ammonia and raises pH, the Fenton reaction is inhibited, reducing the etching of AuNBPs. This approach enables a highly sensitive and distinct multichromatic response across a wide range of urea concentrations, particularly at low target levels.

Layer-controlled continuous MoS growth using a spin-coatable metal precursor buffer.

Continuous large-area MoS growth holds significant potential for next-generation optoelectronic applications, yet achieving it efficiently and reproducibly with the conventional chemical vapor deposition (CVD) process remains a challenge due to inconsistent precursor adsorption and non-uniform nucleation. In this study, we developed a pH-optimized metal precursor buffer that enables uniform spin-coating and facilitates continuous MoS growth. Sodium cholate (SC) acts as both a dispersant and a buffer, transforming molybdenum oxide into a dispersion-stable sodium molybdate (NaMoO)/SC complex.

Insertional Mutations in RsFLC2 Disrupt Vernalization-Induced Intragenic Looping and H3K27me3 Enrichment in Radish.

The floral transition of many winter-annual, biennial, and perennial plants accelerates upon prolonged exposure to winter temperatures, a phenomenon known as vernalization. This study aimed to elucidate the molecular mechanisms underlying vernalization-induced floral transition in radish (Raphanus sativus L.).

Comparison of MRI and prognostic features of intrahepatic cholangiocarcinoma between patients with and without hepatitis B virus infection.

Purpose: To compare the clinical, MRI, and prognostic features of intrahepatic cholangiocarcinoma (ICCA) between patients with and without hepatitis B virus (HBV) infection.

Methods: We retrospectively analyzed 211 patients with ICCA who underwent preoperative MRI and curative-intent surgical resection between 2015 and 2018. Two radiologists independently reviewed MRI features.

Exploiting Brownian Motion of Plasmonic Nanoparticles Using Optical Printing Approach for on-Demand Physical Unclonable Functions.

An on-demand fabrication method for additive physical unclonable functions (PUFs), a hardware-based security primitive, is inevitably required, especially considering increasingly miniaturized microelectronic devices. An optical printing approach is regarded as an alternative method to fabricate functional nano/microscale patterns against conventional methods due to its superior fabrication flexibility. However, owing to the Brownian motion of nanoparticles, achieving highly precise and selective printing persists an ongoing obstacle for the applicability of optical printing methods.

Correction: A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs.

[This corrects the article DOI: 10.1371/journal.pgen.

Ecofriendly Sunlight-Mediated Nontoxic Bimetallic Nanoparticles: Synthesis, Reusable Catalytic Membrane, and Biosensor Applications.

Bimetallic nanoparticles (BMNPs) combine the desirable properties of two distinct metals that outperform conventional monometallic nanoparticles (NPs). This work presents a novel ecofriendly silver-copper (Ag-Cu) BMNPs synthesis using sunlight as a green reducing agent, enableing rapid Ag-Cu BMNPs formation at room temperature within 10 min. This method exploiting the facile reduction of Ag⁺ to Ag⁰, which subsequently mediates the reduction of Cu⁺ to Cu⁰ via water radiolysis-generated species.

Inhibition of GLS1 and ASCT2 Synergistically Enhances the Anticancer Effects in Pancreatic Cancer Cells.

Pancreatic cancer, a leading cause of cancer-related deaths, is characterized by increased dependence on glutamine metabolism. Telaglenastat (CB-839), a glutaminase (GLS) inhibitor targets glutamine metabolism; however, its efficacy as monotherapy is limited owing to metabolic adaptations. In this study, we demonstrated that CB-839 effectively inhibited cell growth in pancreatic cancer cells, but activated the general control nonderepressible 2 (GCN2)-activating transcription factor 4 (ATF4) signaling pathway.

Vertical DNA Nanostructure Arrays: Facilitating Functionalization on Macro-Scale Surfaces.

The capability for varied functionalization and precise control at the nanoscale are significant advantages of DNA nanostructures. In the assembly of DNA nanostructure, the surface-assisted growth method utilizing double-crossover (DX) tile structures facilitates nucleation at relatively low concentrations on the surface based on electrostatic interactions, thereby enabling crystal growth over large areas. However, in surface-assisted growth, the geometrical hindrance of vertical structures on the DX tile structure surface makes it challenging to conjugate DNA nanostructures into fabricated surfaces.

Diagnosis of moderate-to-severe hepatic steatosis using deep learning-based automated attenuation measurements on contrast-enhanced CT.

Purpose: To evaluate the utility of deep learning-based automated attenuation measurements on contrast-enhanced CT (CECT) for diagnosing moderate-to-severe hepatic steatosis (HS), using histology as reference standard.

Methods: This retrospective study included 3,620 liver donors (2,393 men and 1,227 women; mean age, 31.7 ± 9.

MRI-based Deep Learning Algorithm for Assisting Clinically Significant Prostate Cancer Detection: A Bicenter Prospective Study.

Background Although artificial intelligence is actively being developed for prostate MRI, few studies have prospectively validated these tools. Purpose To compare the diagnostic performance of a commercial deep learning algorithm (DLA) and radiologists' clinical reports for cancer detection in participants from two hospitals using histopathologic findings from biopsy specimens as the reference standard. Materials and Methods This prospective bicenter study enrolled participants suspected of having prostate cancer (PCa) who were scheduled for biopsy based on clinical information, including prostate MRI, from December 2022 to July 2023.

Novel approach to upcycling food processing biomass: development of rice snacks using rice bran emulsifier.

This study addresses the need for innovative approaches to upcycling rice bran as a biomass resource. A novel upcycling ingredient derived from rice bran was developed, and its application in the production of rice crackers with enhanced texture was explored. The effects of different emulsifier types and concentrations on the texture, flavor, and overall quality of rice crackers-by influencing the uniformity of oil and moisture in the formulation-were confirmed through systematic analytical methods.

Preoperative Prediction Model for Early Recurrence of Intrahepatic Cholangiocarcinoma After Surgical Resection: Development and External Validation Study.

Purpose: We aimed to develop a preoperative risk scoring system to predict early recurrence (ER) of intrahepatic cholangiocarcinoma (ICCA) after resection, utilizing clinical and computed tomography (CT) features.

Materials And Methods: This multicenter study included 365 patients who underwent curative-intent surgical resection for ICCA at six institutions between 2009 and 2016. Of these, 264 patients from one institution constituted the development cohort, while 101 patients from the other institutions constituted the external validation cohort.