Development of vascularized adipose organoids from PBMSCs and their application in evaluating Celastrol's effects.

Obesity lacks physiologically relevant human models, which constrains in-depth investigation into its underlying mechanisms and hampers the development of effective therapeutic strategies for obesity and its associated comorbidities. Here, we developed vascularized adipose organoids (VAOs) from peripheral blood mesenchymal stem cells (PBMSCs) using a standardized three-dimensional dynamic culture system that sequentially induces angiogenesis and adipogenesis. These VAOs mimic native adipose tissue with coexisting adipocytes and endothelial cells.

Transcriptomic profiling reveals response mechanisms of seedlings to seawater irrigation stress.

Introduction: The increasing global soil salinization has accelerated research on seawater irrigation agriculture. Developing wild vegetables through seawater irrigation could establish foundational strategies for utilizing island vegetable germplasm resources.

Methods: This study investigated two distinct leaf-shaped individuals (S and Y) of wild () through hydroponic experiments with diluted seawater during seedling stage.

Plant growth promoting fungus Cladosporium 'BF-F' promotes cadmium accumulation in Sesuvium portulacastrum by regulating plant metal ion transport.

Cadmium (Cd) inputs in soils have been significantly increased over the last 20 years. Various approaches have been used to remove Cd from contaminated soils, with bioremediation through plants and soil microbes being the most effective. This study investigated interactions of a new plant growth promoting fungus (PGPF), Cladosporium 'BF-F' with Sesuvium portulacastrum plants for bioremediation of Cd in a laboratory setting.

Sex differences in physical activity dose-response effects on site-specific bone mineral density during childhood and adolescence.

Physical activity (PA) serves as a modifiable determinant of bone mineral density (BMD). However, sex- and site-specific dose-response relationships remain poorly defined. Leveraging data from the National Health and Nutrition Examination Survey (NHANES 2011-2014), this cross-sectional study investigated sex-specific associations between accelerometer-derived PA (volume and intensity) and site-specific BMD in 2,659 children and adolescents aged 8-19 years.

Identification of Proteoforms Related to Flower Petaloid Through Proteogenomic Strategy.

is an aquatic plant with a high ornamental value due to its flower. Despite the release of several versions of the lotus genome, its annotation remains inefficient, which makes it difficult to obtain a more comprehensive knowledge when -omic studies are applied to understand the different biological processes. Focusing on the petaloid of the lotus flower, we conducted a comparative proteomic analysis among five major floral organs.

Identification and characterization of two APETALA2 homolog genes in lotus (Nelumbo nucifera) involved in sepal and petal development.

Background: Lotus (Nelumbo nucifera) is a significant aquatic ornamental genus widely utilized in horticulture for its decorative, culinary, medicinal, and other practical uses. It presents a variety of flower shapes, including few-petalled, semi-double-petalled, double-petalled and thousand-petalled flowers, making it an ideal candidate for studying the flower development of aquatic plants. However, the molecular mechanism of floral development in lotus remains elusive.

Enhancing chemical synthesis research with NLP: Word embeddings for chemical reagent identification-A case study on nano-FeCu.

Nanoparticle synthesis is complex, influenced by multiple variables including reagent selection. This study introduces a specialized corpus focused on "Fe, Cu, synthesis" to train a domain-specific word embedding model using natural language processing (NLP) in an unsupervised environment. Evaluation metrics included average cosine similarity, visual analysis via t-distributed stochastic neighbor embedding (t-SNE), synonym analysis, and analogy reasoning analysis.

Transcriptomic and metabolomic analyses reveal mechanisms underpinning resistance of Chinese wild grape to Colletotrichum viniferum.

Grape ripe rot is one of the most important diseases caused by Colletotrichum spp. Chinese wild grape (Vitis davidii) is highly resistant to Colletotrichum viniferum infection. But mechanisms underlying the resistance remain largely unclear.

Proteomic and metabolomic analyses uncover integrative mechanisms in tolerance to salt stress.

Introduction: Salt stress is a major constraint affecting crop productivity worldwide. Investigation of halophytes could provide valuable information for improving economically important crops to tolerate salt stress and for more effectively using halophytes to remediate saline environments. is a halophyte species widely distributed in tropical and subtropical coastal regions and can absorb a large amount of sodium (Na).

A novel endophytic fungus strain of : its identification, genomic analysis, and effects on plant growth.

Introduction: Endophytic microorganisms are bacteria or fungi that inhabit plant internal tissues contributing to various biological processes of plants. Some endophytic microbes can promote plant growth, which are known as plant growth-promoting endophytes (PGPEs). There has been an increasing interest in isolation and identification of PGPEs for sustainable production of crops.

Identification of novel circulating miRNAs biomarkers for healthy obese and lean children.

Background: The prevalence of childhood obesity and overweight has risen globally, leading to increased rates of metabolic disorders. Various factors, including genetic, epigenetic, and environmental influences such as diet and physical activity, contribute to pediatric obesity. This study aimed to identify specific circulating miRNAs as potential biomarkers for assessing obesity in children.

Chronic exercise remodels the lysine acetylome in the mouse hippocampus.

Physical exercise benefits hippocampal function through various molecular mechanisms. Protein acetylation, a conserved and widespread post-translational modification, is involved in the synaptic plasticity and memory. However, whether exercise can change global acetylation and the role of acetylated proteins in the hippocampus have remained largely unknown.

Non-native Plant Species Invasion Increases the Importance of Deterministic Processes in Fungal Community Assembly in a Coastal Wetland.

Fungal communities are essential to the maintenance of soil multifunctionality. Plant invasion represents a growing challenge for the conservation of soil biodiversity across the globe, but the impact of non-native species invasion on fungal diversity, community structure, and assembly processes remains largely unknown. Here, we examined the diversity, community composition, functional guilds, and assembly process of fungi at three soil depths underneath a native species, three non-native species, and a bare tidal flat from a coastal wetland.

Comparative transcriptomic analysis provides insight into carpel petaloidy in lotus ().

Lotus () is a highly recognized flower with high ornamental value. Flower color and flower morphology are two main factors for flower lotus breeding. Petaloidy is a universal phenomenon in lotus flowers.

Genome-wide analysis of AP2/ERF superfamily in lotus (Nelumbo nucifera) and the association between NnADAP and rhizome morphology.

Background: The AP2/ERF family is widely present in plants and plays a crucial regulatory role in plant growth and development. As an essential aquatic horticultural model plant, lotus has an increasingly prominent economic and research value.

Results: We have identified and analysed the AP2/ERF gene family in the lotus.

A CONSTANS-LIKE gene of Nelumbo nucifera could promote potato tuberization.

CONSTANS-LIKE 5 of Nelumbo nucifera is capable of promoting potato tuberization through CONSTANS-FLOWERING LOCUS T and gibberellin signaling pathways with a probable association with lotus rhizome enlargement. Lotus (Nelumbo nucifera) is an aquatic plant that is affiliated to the Nelumbonaceace family. It is widely used as an ornamental, vegetable, and medicinal herb with its rhizome being a popular vegetable.

Genetic dissection of rhizome yield-related traits in Nelumbo nucifera through genetic linkage map construction and QTL mapping.

Lotus (Nelumbo nucifera) is a perennial aquatic plant with great value in ornamentation, nutrition, and medicine. Being a storage organ, lotus rhizome is not only used for vegetative reproduction, but also as a popular vegetable in Southeast Asia. Rhizome development, especially enlargement, largely determines its yield and hence becomes one of the major concerns in rhizome lotus breeding and cultivation.

MicroRNA-302a is involved in folate deficiency-induced apoptosis through the AKT-FOXO1-BIM pathway in mouse embryonic stem cells.

Background: Our previous study had shown that microRNA (miR)-302a played a key role in folate deficiency-induced apoptosis in mouse embryonic stem cells. However, details regarding the mechanism remain unclear. Transcription factors (TFs) and miRNAs are two key elements in gene regulation.

Genome-wide identification of MADS-box gene family in sacred lotus (Nelumbo nucifera) identifies a SEPALLATA homolog gene involved in floral development.

Background: Sacred lotus (Nelumbo nucifera) is a vital perennial aquatic ornamental plant. Its flower shape determines the horticultural and ornamental values. However, the mechanisms underlying lotus flower development are still elusive.

Integrated Omics Analyses Identify Key Pathways Involved in Petiole Rigidity Formation in Sacred Lotus.

Sacred lotus ( Gaertn.) is a relic aquatic plant with two types of leaves, which have distinct rigidity of petioles. Here we assess the difference from anatomic structure to the expression of genes and proteins in two petioles types, and identify key pathways involved in petiole rigidity formation in sacred lotus.

Lipid-coated ZnO nanoparticles synthesis, characterization and cytotoxicity studies in cancer cell.

ZnO nanoparticles are widely used in biological, chemical, and medical fields, but their toxicity impedes their wide application. In this study, pristine ZnO NPs (~ 7 nm; ~ 18 nm; ~ 49 nm) and lipid-coated ZnO NPs (~ 13 nm; ~ 22 nm; ~ 52 nm) with different morphologies were prepared by chemical method and characterized by TEM, XRD, HRTEM, FTIR, and DLS. Our results showed that the lipid-coated ZnO NPs (~ 13 nm; ~ 22 nm; ~ 52 nm) groups improved the colloidal stability, prevented the aggregation and dissolution of nanocrystal particles in the solution, inhibited the dissolution of ZnO NPs into Zn cations, and reduced cytotoxicity more efficiently than the pristine ZnO NPs (~ 7 nm; ~ 18 nm; ~ 49 nm).

Proteomic analysis showing the signaling pathways involved in the rhizome enlargement process in Nelumbo nucifera.

Background: Rhizome is the storage underground stem of lotus (Nelumbo nucifera), which is enlarged before winter season and could be used for asexual propagation. In addition, the enlarged rhizome is a nutritional vegetable with abundant starch, proteins, and vitamins. Enlargement of lotus rhizome is not only significance for itself to survive from the cold winter, but also important for its economic value.

The Latest Studies on Lotus ()-an Emerging Horticultural Model Plant.

Lotus () is a perennial aquatic basal eudicot belonging to a small family , which contains only one genus with two species. It is an important horticultural plant, with its uses ranging from ornamental, nutritional to medicinal values, and has been widely used, especially in Southeast Asia. Recently, the lotus obtained a lot of attention from the scientific community.

Preparation of ZnO Nanoparticles with High Dispersibility Based on Oriented Attachment (OA) Process.

Understanding nanoparticle growth mechanisms is crucial for the synthesis of nanocrystals with desired biological and chemical properties. Growth of nanocrystals by oriented attachment (OA) is frequently reported as a method supplementary to the classical growth by Ostwald ripening (OR) process. In this work, ZnO nanoparticles (NPs) were prepared by wet chemical method.