Spatially resolved lipid disruption induced in Daphnia magna by environmentally relevant exposure to obesogens tributyltin and pyriproxyfen.

Environmental pollutants, particularly endocrine disruptors, are known to significantly dysregulate lipid homeostasis, causing severe health issues. However, their obesogenic effects on invertebrate species, such as the planktonic crustacean Daphnia magna, remain poorly understood. Previous research suggested that compounds such as organotin tributyltin and the insecticide pyriproxyfen promote lipid accumulation, particularly triacylglycerol species.

Substantiating chemical groups for read-across using molecular response profiles.

By grouping structurally similar chemicals, toxicity endpoints from data-rich substances can be read across to data-poor substances, supporting environmental and human health risk assessment without animal testing. However, structural similarity alone is insufficient, and additional supporting data can strengthen a grouping justification. This study aimed to demonstrate how multi-omics bioactivity data can increase confidence in a grouping hypothesis, where the bioactivity profiles can reflect a chemical's mode(s) of action.

Assessing neurological and cardiovascular effects caused by pharmaceuticals in river water: Insights from Daphnia magna and Danio rerio embryos.

Pharmaceutical residues in surface waters are an emerging environmental and public health issue, yet their biological impacts on aquatic life remain poorly understood. This study presents a cost-effective bioanalytical framework using Daphnia magna juveniles and zebrafish (Danio rerio) embryos to evaluate neurotoxic and cardiotoxic effects of pharmaceutical mixtures in rivers downstream of wastewater treatment plant (WWTP) effluents. Water samples from three rivers in north-eastern Spain (Besòs, Llobregat, and Onyar) were concentrated up to 5- and 20-fold using solid-phase extraction.

Effects of water temperature increase on gene expression, biochemical, and histopathological responses of the bivalve species Mytilus galloprovincialis to the antineoplastic drug 5-fluorouracil.

The present study aimed to assess the potential ecotoxicological effects of 5-fluorouracil (5-FU), an antineoplastic already found in aquatic ecosystems, towards the bivalve Mytilus galloprovincialis, considering actual and warming temperature scenarios. To this end, organisms were exposed for 28 days to increasing 5-FU concentrations (10, 100, and 500 ng/L) at control (17 ± 1.0 °C) and warming (21 ± 1.

Micro-bioplastic impact on gut microbiome, cephalic transcription and cognitive function in the aquatic invertebrate Daphnia magna.

The role of the gut microbiome-brain axis on contaminant effects in invertebrates is limited by our poor knowledge of gut microbiome neurological regulatory pathways. This study investigates the influence of microplastics on the gut microbiome composition and assess subsequent alterations in the cephalic transcriptome, feeding patterns, and overall behaviour of the organism. D.

Validation of a large volume-solid phase extraction methodology for biotoxicity assessment of pharmaceuticals in aquatic organisms.

The growing scarcity of freshwater has intensified the need for alternative water sources, which often require advanced treatments to eliminate contaminants. Among emerging pollutants, pharmaceuticals have become a significant concern due to their persistence and potential impact, making their detection essential. However, analysing these contaminants is challenging due to the extremely low concentrations at which they are present.

Ultra-sensitive UHPLC-MS/MS method for simultaneous quantification of 31 neurochemicals in zebrafish larvae and brain.

The precise determination of neurotransmitters and their metabolites in biological matrices is critical for research on neurological disorders, including those originated by the exposure to neurotoxic chemicals. This study presents an optimized liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for 31 neurochemicals, including neurotransmitters, their metabolites and precursors. The method is aimed at achieving lower limits of detection (LOD) and quantification (LOQ) compared to those currently available, while simultaneously expanding the number of compounds analyzed.

Mass spectrometry imaging enables detection of MPs and their effects in Daphnia magna following acute exposure.

Microplastics (MPs) are continuously found in soil and water environments. Within aquatic ecosystems, filter-feeding organisms are unable to discriminate MPs from food particles while fish may intentionally ingest MPs by mistaking them for prey. In both cases, MPs can accumulate in tissues with subsequent implications for human and environmental health.

Molecular mechanisms that regulate scopolamine effects on inducible fish antipredation responses in Daphnia magna.

Daphnia's antipredator responses are regulated largely by the nervous system, making these responses particularly susceptible to compounds that impact neurodevelopmental or neurofunctional processes. This study aimed to determine the molecular pathways involved in modulating the effects of scopolamine on inducible antipredation responses triggered by fish kairomones. We used two clones showing two contrasting responses.

Zebra_K, a kinematic analysis automated platform for assessing sensitivity, habituation and prepulse inhibition of the acoustic startle response in adult zebrafish.

The acoustic startle response (ASR) is leaded by a sudden and intense acoustic stimulus. ASR has several forms of plasticity, including habituation and sensorimotor gating. Although ASR and its plasticity have been intensively studied in zebrafish (Danio rerio) larvae, information in adult zebrafish is still very scarce.

Primary Biliary Cholangitis and Chronic Arterial Hypertension in Pregnancy.

This case report presents a woman with a history of adverse obstetric outcomes: two pregnancies complicated by preeclampsia, resulting in a medical termination in the first and fetal demise in the second. Prior to a subsequent pregnancy, she underwent investigations for hypertension, thrombophilia, and autoimmune diseases. These investigations led to a probable diagnosis of primary biliary cholangitis (PBC) and chronic hypertension (CH).

Biomonitoring emerging hazards of pharmaceuticals in river water using gut microbiome and behavioural Daphnia magna responses.

A cost-effective Daphnia magna testing framework was applied to identify emerging hazards such as neurological and cardiovascular defects as well as antibiotic resistant genes (ARGs), related to pharmaceuticals present in waste water treated (WWTP) effluent discharged into rivers. D. magna juveniles were exposed during 48 h to water samples from three rivers in the vicinity of Barcelona (NE Spain), Besós, Llobregat and Onyar, upstream and downstream of WWTP discharging points.

Salinity influence on Mytilus galloprovincialis exposed to antineoplastic agents: a transcriptomic, biochemical, and histopathological approach.

Nowadays, aquatic species face a variety of environmental risks associated with pharmaceutical consumption. More specifically, the increased number of cancer patients has been accompanied by an increased consumption of antineoplastic drugs, such as ifosfamide (IF) and cyclophosphamide (CP). These drugs have been found in aquatic ecosystems, raising concerns about their impact, especially on estuarine species, as marine waters are the final recipients of continental effluents.

Facilitating microplastic ingestion in aquatic models: A verified protocol for daphnia magna as a trojan horse vector.

Microplastic pollution poses a significant environmental threat due to its persistence, widespread distribution, and inherent toxic potential. Despite the increasing number of publications in this field, a standardized protocol for the laboratory intake of microplastics by has yet to be established. In this study, we introduce a verified protocol designed to facilitate the ingestion of microplastic particles (MPs) by , ranging in size from 5-55 µm.

From Ovarian Hyperstimulation to the Discovery of a Liver Nodule.

Ovarian hyperstimulation syndrome (OHSS) is a complication of ovulation induction. Deep vein thrombosis (DVT) can occur as a consequence of this syndrome, but it is an infrequent event. The authors describe the case of a woman who became pregnant after ovulation induction and developed severe OHSS and, subsequently, DVT of the right brachiocephalic trunk, internal and external jugular veins, and right subclavian vein.

Environmental risk assessment of pharmaceuticals in wastewaters and reclaimed water from catalan main river basins.

Aquatic pollution from pharmaceuticals is a growing environmental concern globally, particularly in Catalonia's primary water bodies, the Llobregat and Besòs rivers. This study investigates pharmaceutical residues in reclaimed water effluents from the Llobregat River and a wastewater treatment plant (WWTP) in the Besòs River, critical contributors to the region's water resources. Employing LC-MS/MS, 85 pharmaceutical residues were monitored, revealing elevated concentrations of tramadol, losartan, and gemfibrozil, commonly prescribed drugs in Catalonia.

Unravelling biochemical responses in the species Mytilus galloprovincialis exposed to the antineoplastics ifosfamide and cisplatin under different temperature scenarios.

This study investigates the chronic impact of two of the most widely consumed antineoplastic drugs, Ifosfamide (IF) and Cisplatin (CDDP), on the bivalve species Mytilus galloprovincialis under current (17 °C) and predicted warming conditions (21 °C). Accompanying the expected increase in worldwide cancer incidence, antineoplastics detection in the aquatic environment is also expected to rise. Mussels were exposed to varying concentrations of IF (10, 100, 500 ng/L) and CDDP (10, 100, 1000 ng/L) for 28 days.

Analyzing the Effects of Age, Time of Day, and Experiment on the Basal Locomotor Activity and Light-Off Visual Motor Response Assays in Zebrafish Larvae.

The recent availability of commercial platforms for behavioral analyses in zebrafish larvae based on video-tracking technologies has exponentially increased the number of studies analyzing different behaviors in this model organism to assess neurotoxicity. Among the most commonly used assays in zebrafish larvae are basal locomotor activity (BLA) and visual motor responses (VMRs). However, the effect of different intrinsic and extrinsic factors that can significantly alter the outcome of these assays is still not well understood.

Assessing Environmental Risks during the Drug Development Process for Parasitic Vector-Borne Diseases: A Critical Reflection.

Parasitic vector-borne diseases (VBDs) represent nearly 20% of the global burden of infectious diseases. Moreover, the spread of VBDs is enhanced by global travel, urbanization, and climate change. Treatment of VBDs faces challenges due to limitations of existing drugs, as the potential for side effects in nontarget species raises significant environmental concerns.

Cardiac and neurobehavioral impairments in three phylogenetically distant aquatic model organisms exposed to environmentally relevant concentrations of boscalid.

Boscalid (2-Chloro-N-(4'-chlorobiphenyl-2-yl) nicotinamide), a pyridine carboxamide fungicide, is an inhibitor of the complex II of the respiration chain in fungal mitochondria. As boscalid is only moderately toxic for aquatic organisms (LC > 1-10 mg/L), current environmental levels of this compound in aquatic ecosystems, in the range of ng/L-μg/L, are considered safe for aquatic organisms. In this study, we have exposed zebrafish (Danio rerio), Japanese medaka (Oryzias latipes) and Daphnia magna to a range of concentrations of boscalid (1-1000 μg/L) for 24 h, and the effects on heart rate (HR), basal locomotor activity (BLA), visual motor response (VMR), startle response (SR), and habituation (HB) to a series of vibrational or light stimuli have been evaluated.

Developmental defects in cognition, metabolic and cardiac function following maternal exposures to low environmental levels of selective serotonin re-uptake inhibitors and tributyltin in Daphnia magna.

Aquatic organisms are exposed to low concentrations of neuro-active chemicals, many of them acting also as neuroendocrine disruptors that can be hazardous during earlier embryonic stages. The present study aims to assess how exposure early in live to environmental low concentrations of two selective serotonin reuptake inhibitors (SSRIs), fluoxetine and sertraline, and tributyltin (TBT) affected cognitive, metabolic and cardiac responses in the model aquatic crustacean Daphnia magna. To that end, newly brooded females were exposed for an entire reproductive cycle (3-4 days) and the response of collected juveniles in the first, second and third consecutive broods, which were exposed, respectively, as embryos, provisioned and un-provisioned egg stages, was monitored.

From biomarkers to community composition: Negative effects of UV/chlorine-treated reclaimed urban wastewater on freshwater biota.

The use of urban wastewater reclaimed water has recently increased across the globe to restore stream environmental flows and mitigate the effects of water scarcity. Reclaimed water is disinfected using different treatments, but their effects into the receiving rivers are little studied. Physiological bioassays and biomarkers can detect sub-lethal effects on target species, but do not provide information on changes in community structure.

Short-term exposure to environmental levels of nicotine and cotinine impairs visual motor response in zebrafish larvae through a similar mode of action: Exploring the potential role of zebrafish α7 nAChR.

The current view is that environmental levels of nicotine and cotinine, commonly in the ng/L range, are safe for aquatic organisms. In this study, 7 days post-fertilization zebrafish embryos have been exposed for 24 h to a range of environmental concentrations of nicotine (2.0 ng/L-2.

Non-target ROIMCR LC-MS analysis of the disruptive effects of TBT over time on the lipidomics of Daphnia magna.

Introduction: This study has investigated the temporal disruptive effects of tributyltin (TBT) on lipid homeostasis in Daphnia magna. To achieve this, the study used Liquid Chromatography-Mass Spectrometry (LC-MS) analysis to analyze biological samples of Daphnia magna treated with TBT over time. The resulting data sets were multivariate and three-way, and were modeled using bilinear and trilinear non-negative factor decomposition chemometric methods.