Lipase production from VC-6 isolated from the volcanic region of Copahue: optimization and functional genomic insights.

Extremophilic microorganisms produce highly stable and industrial-grade enzymes with enhanced performance. Thermostable enzymes, such as lipases that catalyze the hydrolysis and esterification of lipids, are of great industrial interest due to their stability and efficacy under harsh conditions, making them ideal for applications in biotechnology, pharmaceuticals, and cosmetics. Lipase production from various microorganisms is well-studied.

A Bibliometric Analysis of 50 Years of Publication of Nurse Educator.

Background: Nurse Educator has been published for 50 years, but no bibliometric studies have been done.

Purpose: The aim was to analyze the focus and characteristics of articles in Nurse Educator through its 50-year history.

Methods: This was a bibliometric analysis of the journal.

Implementing a prebrief for cultural humility in standardized patient sessions with genetic counseling students.

Standardized patient (SP) sessions allow students to practice cultural humility and gain confidence in providing care for diverse patient populations. A prebrief (Pb) occurs before participation in the SP session and involves three steps: planning, briefing, and facilitating. Prebriefing is effective in integrating cultural humility in healthcare education fields, such as nursing.

Thelazia callipaeda (Spirurida, Thelaziidae): First record of an imported ocular parasite in dogs from the Canary Islands, Spain.

The ocular parasite Thelazia callipaeda is a nematode transmitted by dipterans of the genus Phortica that affects various species of domestic and wild carnivores and, sporadically, humans. Although the presence of this parasite has been widely documented on the Iberian Peninsula, no cases have been reported in the Canary Islands (Spain). In the present study, ocular parasitosis in dogs from Tenerife and Gran Canaria are reported.

Two stages of substrate discrimination dictate selectivity in the Escherichia coli MetNI-Q ABC transporter system.

The Escherichia coli MetNI-Q importer, an ATP-binding cassette (ABC) transporter, mediates the uptake of both L- and D-enantiomers of methionine. The original in vivo uptake studies show a strong preference for L-Met over D-Met, but the molecular basis of this selectivity is unclear. In this work, we systematically examine substrate discrimination by the MetNI transporter and MetQ substrate-binding protein using an array of biophysical and biochemical techniques.

Microsporidia in Rodents-, and -A Public Health Concern in the Canary Islands, Spain.

Rodents are recognized as reservoirs of a wide range of pathogens, including microsporidia. The presence of microsporidia in the environment of mainland Spain and its islands has become increasingly known, as the number of studies has multiplied over time. The present study was conducted to determine the occurrence and diversity of microsporidia in three rodent species (, , and ) in the Canary Islands, Spain.

Cultural capacity, self-efficacy, and humility of healthcare providers: a survey in Southeast Iran.

Background: With the increasing cultural diversity in healthcare settings, it is essential for healthcare providers to possess the three key characteristics of cultural capacity, self-efficacy, and humility in order to deliver optimal and culturally congruent care. The present study aimed to assess the relationship between cultural capacity, self-efficacy, and humility among healthcare providers.

Method: This cross-sectional study used a convenience sampling method to include 203 healthcare providers from comprehensive health centers and facilities affiliated with Kerman University of Medical Sciences in Kerman, southeastern Iran.

Unlocking public health competencies: the dose-response effect of problem-based learning on undergraduate student outcomes.

Introduction: Problem-based learning (PBL) is a student-centered pedagogical strategy that emphasizes active learning through the exploration of complex real-world problems. While it has demonstrated effectiveness in undergraduate- and graduate-level programs, there is a notable gap in research on the dose-response relationship between the extent of PBL exposure and competency development in public health education. This study examines the effect of PBL on undergraduate public health students' learning outcomes and explores the dose-response relationship between the extent of PBL exposure and the development of specific public health competencies in students.

Teaching Truth in Turbulent Times: Navigating Politicized Policies on Sex and Gender in Nursing Education.

Background: Nurse educators are confronted with legislative measures that narrowly define sex and gender, potentially excluding transgender and non-binary identities from nursing curricula. This article examines challenges when federal policy directives conflict with scientific consensus and propose strategies for preserving quality education.

Problem: Restrictive policies threaten academic freedom, undermine scientific rigor, and compromise ethical obligations to deliver inclusive, culturally humble care for diverse patients.

New data on the catenotaeniid cestode (Mikhail and Fahmy, 1968) parasitizing Fat sand rats, (Muridae: Gerbillinae), in Tunisia.

The cestode (Cyclophyllidea: Catenotaeniidae: Skrjabinotaeniinae) was found parasitizing fat sand rats () in the south of Tunisia. The species was found previously in from Egypt and in from Morocco. The present finding constitutes the first record of the species in Tunisia.

Unusual Mass Mortality of Atlantic Puffins () in the Canary Islands Associated with Adverse Weather Events.

The Atlantic puffin () is a seabird species characterized by great diving capabilities and transoceanic migratory behavior. These movements contribute to the dispersion of the species during migration, and episodes of mortality associated with migration may be a normal event in the dynamic of the Atlantic puffin populations. This study aimed to describe the anatomopathological findings of an unusual mortality event of Atlantic puffins observed during the non-breeding period along the coast of the Canary Islands.

Chemical Recognition Cues in Ant-Aphid Mutualism: Differentiating, Sharing, and Modifying Cuticular Components.

Aphid-tending ants form mutualistic associations with aphids. During their interactions, aphids and ants use both tactile stimuli and chemical cues to communicate. Recent studies suggest that ants modify the cuticular hydrocarbons of mutualistic aphids they attend, but it is unclear which compounds are implicated in recognition.

Enhancing Asthma Management Among Haitian Caregivers: A Quality Improvement Initiative.

Aim: Asthma disparities persist among Haitian children living in the United States. Language and cultural barriers contribute to increased hospitalisations and emergency department visits. The goal of this project is to offer culturally sensitive and linguistically appropriate education to Haitian parents and caregivers of children with asthma featuring a personalised asthma action plan (AAP).

Comparative larval anatomy of the digestive system of three Calliphoridae (Diptera) species that cause different types of myiasis.

The Calliphoridae are one of the main Diptera families that include agents of the parasitic disease condition known as myiasis. Parasitism seems to have evolved multiple independent times within the Calliphoridae; consequently, this family includes a diversity of myiasis-causing species, varying in their obligate or facultative habits and in their specific location in the host. Larval morphological studies can provide novel and relevant insights into the biology of those species, as well as on the pathogenesis and evolution of myiasis; however, the anatomy of internal organs and structures -particularly those within the digestive system- has generally been overlooked, despite potentially reflecting parasitic adaptations.

A Qualitative Study Exploring Dietetic Students' and Registered Dietitians' Views on Cultural Humility in Training and the Profession.

Introduction: Cultural humility is a critical component of equitable and inclusive nutrition care. The current study aimed to explore the existing state of cultural competence among nutrition students and nutrition professionals while identifying the need for and acceptability of cultural humility training tailored for nutrition professionals.

Methods: Dietetic students and nutrition professionals from various educational and professional backgrounds in the United States were recruited through purposive sampling.

National survey of the rat hepatitis E virus in rodents in Spain, 2022 to 2023.

BackgroundRat hepatitis E virus (ratHEV) is an emerging virus causing acute and chronic hepatitis in humans. Rats are the main natural reservoir of this pathogen. Large-scale studies assessing ratHEV circulation in rodents in Spain are lacking.

A potent and selective TNKS2 inhibitor for tumor-selective WNT suppression.

Hyperactive WNT signaling is a potent cancer driver, but clinical translation of WNT inhibitors has been hampered by on-target toxicities. WNT signaling can be constrained through inhibition of the PARP family enzymes Tankyrase 1 (TNKS1) and Tankyrase 2 (TNKS2), however, existing TNKS inhibitors suppress WNT signaling in both tumor and healthy tissues. In this study, we show that the loss of chromosome 8p that occurs in approximately half of advanced epithelial malignancies, creates a collateral vulnerability that enables tumor-selective inhibition of Tankyrase activity.

Implementation of Perioperative Anesthesia Considerations for Military Veterans Who Consume Cannabis: A Quality Improvement Project.

Purpose: As no widely accepted recommendations or guidelines on perioperative management for the cannabis-consuming patient exist, this quality improvement project aimed to identify and implement evidence-based recommendations for cannabis-consuming patients throughout the perioperative period. The objectives of this project were (1) to improve anesthesia providers' knowledge on how to care for cannabis-consuming patients, (2) to increase anesthesia providers' self-efficacy in caring for cannabis-consuming patients, and (3) to assess the frequency of utilization of the given evidence-based recommendation.

Design: This quality improvement project incorporated a pretest-posttest design.

Use of Text Messaging to Educate Family Caregivers of Children With Asthma.

Purpose: Knowledge deficiencies among family caregivers of children with asthma impede optimal disease management. Current research has shown that texting caregivers is an effective method for delivering family-centered health education. The aim of this quality improvement project was to implement a short message service (SMS)-based intervention to educate family caregivers of children with asthma and evaluate its effect on caregiver knowledge and confidence in asthma management.

Two stages of substrate discrimination dictate selectivity in the MetNI-Q ABC transporter system.

The MetNI-Q importer, an ATP-binding cassette (ABC) transporter, mediates the uptake of both L- and D- enantiomers of methionine. Original uptake studies show a strong preference for L-Met over D-Met, but the molecular basis of this selectivity is unclear. In this work, we systematically examine substrate discrimination by the MetNI transporter and MetQ substrate binding protein using an array of biophysical and biochemical techniques.

Racism in clinical nursing practice: a qualitative study.

Background: Racism in healthcare has led to disparate health outcomes amongst people of color. The construct of racism may be misunderstood, and research is lacking about the actions nurses can in the clinical setting take to reduce racism. The purpose of the study was to determine behaviors demonstrative of racism in nursing care and behaviors that are demonstrative of culturally humble nursing care to develop an awareness of racial bias in nursing to inform future educational practices.

Ambivalent partnership of the Drosophila posterior class Hox protein Abdominal-B with Extradenticle and Homothorax.

Hox proteins, a sub-group of the homeodomain (HD) transcription factor family, provide positional information for axial patterning in development and evolution. Hox protein functional specificity is reached, at least in part, through interactions with Pbc (Extradenticle (Exd) in Drosophila) and Meis/Prep (Homothorax (Hth) in Drosophila) proteins. Most of our current knowledge of Hox protein specificity stems from the study of anterior and central Hox proteins, identifying the molecular and structural bases for Hox/Pbc/Meis-Prep cooperative action.

Tracking Abdominal-B Expression and Function in the Fly Internal Reproductive System by Explants Imaging.

Hox genes specify identities mainly in the anteroposterior axis in various animal tissues, some of them forming part of the internal organs and systems. The expression and activity of these genes have been analyzed mainly in Drosophila melanogaster, the fruit fly, and in mouse; in the former, the functional study of Hox genes has been detailed predominantly in epidermal structures, but their role in internal organs poses some challenges, particularly in pupae. One of these genes, Abdominal-B, dictates the development of many internal organs in the posterior abdomen of the fly, yet techniques for its analysis, like in vivo time-lapse, have long been impractical.