3D-Printed Biocompatible Frames for Electrospun Nanofiber Membranes: An Enabling Biofabrication Technology for Three-Dimensional Tissue Models and Engineered Cell Culture Platforms.

Electrospun nanofiber membranes (ESNFMs) are exceptional biomaterials for tissue engineering, closely mimicking the native extracellular matrix. However, their inherent fragility poses significant handling, processing, and integration challenges, limiting their widespread application in advanced 3D tissue models and biofabricated devices. This study introduces a novel and on-mat framing technique utilizing extrusion-based printing of a UV-curable biocompatible resin (Biotough D90 MF) to create rigid, integrated support structures directly on chitosan-polyethylene oxide (PEO) ESNFMs.

RNA Sequencing on Muscle Biopsies from Exertional Rhabdomyolysis Patients Revealed Down-Regulation of Mitochondrial Function and Enhancement of Extracellular Matrix Composition.

Background/objective: Exertional rhabdomyolysis (ER) is primarily driven by mechanical stress on muscles during strenuous or unaccustomed exercise, often exacerbated by environmental factors like heat and dehydration. While the general cellular pathway involving energy depletion and calcium overload is understood in horse ER models, the underlying mechanisms specific to the ER are not universally known within humans. This study aimed to evaluate whether patients with ER exhibited transcriptional signatures that were significantly different from those of healthy individuals.

Neutrophil Gelatinase-Associated Lipocalin: A Shared Early Biomarker of Remote Organ Dysfunction in Blast-Induced Extremity Trauma.

Polytrauma is a critical global health concern characterized by immune dysregulation and a high risk of multiple organ dysfunction syndrome (MODS). Early molecular mechanisms linking trauma severity to organ injury are poorly understood. We used two rat blast-polytrauma models: a tourniquet-induced ischemia/reperfusion injury (tIRI) model and a non-ischemia/reperfusion injury (non-IRI) model.

User experience and hemostatic efficacy: Comparative analysis of commercial agents in junctional and hepatic hemorrhage models.

Background/objectives: Hemorrhage is associated with most preventable combat-related deaths. Management of non-compressible truncal and junctional hemorrhage remains challenging, especially with prolonged evacuations. This study evaluated the efficacy of commercial topical hemostatic agents in uncontrolled hemorrhage models under coagulopathic conditions, examining differences based on applicator experience.

New Approaches to Investigate Novel Agents in Ewing Sarcoma: A Report From the Children's Oncology Group Bone Tumor Committee.

Outcomes for relapsed Ewing sarcoma remain consistently poor. Continued efforts to consider creative new approaches for the treatment of relapsed Ewing sarcoma are needed. The Children's Oncology Group Bone Tumor Committee convened a New Strategies for Ewing Sarcoma Task Force to systematically evaluate agents for inclusion in future Phase II or III clinical trials for relapsed Ewing sarcoma.

The Effect of Experimentally Induced Cognitive Fatigue on Energy Intake Among Youth With and Without Recent Reported Dietary Restraint.

Objective: Resource-based models of self-regulation posit that cognitive fatigue, or the depletion of cognitive resources, may be an impetus for self-regulatory difficulties, including overeating. Studies of adults indicate that cognitive fatigue may increase energy intake only among adults who report engaging in dietary restraint (DR). The current study examined if DR similarly moderates the effects of cognitive fatigue on energy intake in youth.

Evaluating the muscularity-oriented eating test: Factor structure and psychometric properties among university athletes and across sexes.

Objective: The Muscularity-Oriented Eating Test (MOET) was developed to measure muscularity-oriented eating pathology among young men; however, this construct is relevant across sexes and may be particularly applicable to athletes. Thus, this study sought to validate the MOET among male and female athletes and non-athletes.

Methods: Students (N = 2189) from three U.

Early Factors Related to Healthcare Utilization by Infection Status among Combat Injured.

Introduction: Combat casualty care is resource-intensive; however, the impact of battlefield-related infections on healthcare utilization is not fully understood. We assessed factors associated with hospitalization among wounded military personnel by infection outcome.

Materials And Methods: The study population for this cross-sectional retrospective analysis included military personnel wounded during deployment (June 1, 2009-December 31, 2014), admitted to Landstuhl Regional Medical Center (Germany), and transferred to participating military hospitals in the continental United States.

Malaria Incidence in US Military Families Is Related to Service Member's Birthplace.

Background: Foreign-born persons make up approximately 5% of the United States military active duty service members. Studies in the general population show that travelers visiting friends and relatives have an increased risk of malaria acquisition. We hypothesized a higher incidence of malaria within the Military Health System (MHS) for those with a familial connection to malaria-endemic countries.

Unhealthy family functioning is strongly associated with warfighter brain health following traumatic brain injury in United States service members and veterans.

Objective: To examine the relationship between family functioning and health-related quality of life (HRQOL) outcomes following traumatic brain injury (TBI) in service members and veterans (SMVs).

Participants: Participants were 359 United States SMVs classified into three groups: non-injured controls (NIC,  = 62); uncomplicated mild TBI (MTBI;  = 189); and complicated mild, moderate, severe, and penetrating TBI (STBI;  = 108). Participants completed 10 HRQOL measures from the TBI-QOL and Neuro-QOL, and the Family Assessment Device-General Functioning subscale (FAD-GF) 2-or-more years post-injury.

The interaction between neck pain and known determinates of delayed return to play among NCAA student-athletes: a CARE consortium study.

Background: Acute neck pain following a concussion has been identified as a symptom associated with a delay in symptom resolution and persistent post-concussion symptoms (PPCS), but little is known about the relationship between the presence of acute neck pain and other known risk factors of delayed return to play (RTP) such as sex, sport contact level, and race.

Methods: This study reports the findings of a secondary data analysis of a prospective cohort study sample from the Concussion Assessment, Research, and Education (CARE) Consortium. We examined the possible mediating effects of neck pain severity on known factors of delayed RTP (sex, sport contact level, and race) following a suspected concussion.

Editorial Commentary: Response to intraarticular injection may not predict response to hip arthroscopy, but don't put down that needle quite yet.

Mounting evidence suggests that patient response to intraarticular injection may not predict clinical response to arthroscopy in the setting of femoroacetabular impingement syndrome (FAIS) as once believed. However, the hip preservation community should not take this to mean that diagnostic injections lack value in establishing a diagnosis of FAIS and distinguishing it from extraarticular hip conditions. Intraarticular injection, like other diagnostic tools, is not a stand-alone test intended to definitively determine proper treatment.

Serum Metabolomic Signatures in Nonhuman Primates Treated with a Countermeasure and Exposed to Partial- or Total-Body Radiation.

: Irradiation-induced injury is a common fallout of radiological/nuclear accidents or therapeutic exposures to high doses of radiation at high dose rates. Currently, there are no prophylactic drugs available to mitigate radiation injury as a result of exposure to lethal doses of ionizing radiation. Gamma-tocotrienol (GT3) of vitamin E is a promising radioprotector under advanced development which has been tested for efficacy in both murine and nonhuman primate (NHP) models.

Characterization of rhesus macaque model for cobalt-60 gamma-radiation source without use of blood product.

Despite significant radiobiological advancements following World War II, only a limited number of medical countermeasures (MCMs) have been approved by the United States Food and Drug Administration (US FDA) for acute radiation exposure related illnesses. Accordingly, well-characterized and validated animal models, both large and small, are still very much needed to develop safe and effective countermeasures. Animal models that are used for such purposes need to reflect not only the clinical and pathogenic features of those seen in radiation exposed humans, but also comparable radiation dose- and time-dependent relationships.

Brief Manual for Multi-Modal Motion-Assisted Memory Desensitization and Reconsolidation Therapy for the Treatment of Post-traumatic Stress Disorder.

Multi-Modal Motion-Assisted Memory Desensitization and Reconsolidation (3MDR) is an innovative exposure-based immersive psychotherapeutic intervention for the treatment of post-traumatic conditions such as post-traumatic stress disorder (PTSD) and other related trauma disorders. This manual reviews the theoretical foundations, protocol, and key therapeutic processes of 3MDR, emphasizing its applicability across clinical and research settings. Developed to overcome barriers to traditional trauma-focused psychotherapies, 3MDR combines immersive virtual reality (VR), motion-assisted engagement, and personalized trauma cues to facilitate memory processing and emotional reconsolidation.

Using Pretrained Large Language Models for AI-Driven Assessment in Medical Education.

ProblemAssessing students in competency-based medical education can be time-consuming and demanding for faculty, especially with large classes and complex topics. Traditional methods can lead to inconsistencies and a lack of targeted feedback. Innovative and accessible solutions to improve the efficiency, objectivity, and effectiveness of assessment in medical education are needed.

Linguistic Analysis of Patients' Labels During 3MDR Psychotherapy.

Background: This study explores the role of linguistic labels expressed by patients during Motion-assisted Multi-modal Memory Desensitisation and Reconsolidation (3MDR) therapy for post-traumatic stress disorder (PTSD). Labels represent a patient's key emotional, cognitive, and physical reactions to images associated with traumatic experiences. By analyzing labels, the goal is to provide insights into their progress throughout the therapeutic process.

Motion-Assisted, Multi-Modal Memory Desensitization and Reconsolidation Therapy for Posttraumatic Stress Disorder in Women with a History of Sexual Trauma.

Background: Women military service members (SMs) are more likely to have posttraumatic stress disorder (PTSD) related to sexual assault, highlighting a need for the development and validation of therapies. A new exposure-based therapy called motion-assisted, multi-modal memory desensitization and reconsolidation (3MDR) uses participant-chosen music and images and an eye movement (EM) task in a virtual environment. Motion-assisted, multi-modal memory desensitization and reconsolidation has shown effectiveness in treating treatment-resistant male veterans; thus, this paper focuses expressly on the utility of 3MDR in female study participants, who were 50% of the full study population.

An Anesthesiologist's Moral Distress With Organ Procurement: A Narrative Review.

Organ procurement procedures have evolved significantly over the past decade, resulting in new moral uncertainties. The dead donor rule (DDR), a foundational principle, mandates that organ procurement occurs only after death is confirmed. However, developments such as donation after circulatory death (DCD) and normothermic regional perfusion (NRP) have raised ethical issues, creating moral distress and moral injury among anesthesiologists.

Data Saves Lives-The U.S. Military Must Build a Real-Time Combat Casualty Care Data Ecosystem for the Future Battlefield.

Large-scale combat operations (LSCO) will challenge the current U.S. military trauma system with high casualty volumes, prolonged evacuation timelines, and degraded logistics.

The Impact of a Unique Military Treatment Facility-Based Sports Medicine Model on Readiness in the Army: A Prospective Longitudinal Analysis Comparing 2 Military Health System Entry Portal Pathways on the Duration of Healthcare Management and the Incidence of Recurrence Within 24 Months.

Objective: Describe an innovative Sports Medicine clinic design implemented in an outpatient Physical Therapy (PT) clinic within a Military Treatment Facility (MTF) for both referral and direct access (DA) entry portal pathways, and to investigate the modifying effect of medical system initial entry pathway for musculoskeletal injury (MSI) on time from injury to begin care for outcomes of duration of care, recurrence of MSI care for the same condition out to 2 years from discharge after initial care, and physical profile status at time of initial discharge.

Design: Quasi-experimental prospective longitudinal cohort study of active duty Army Soldiers in a MTF who sought care for an initial MSI over a 2-year period.

Materials And Methods: Four hundred eighty-eight individuals were classified by the pathway they entered the Military Health System through: early access pt (EAPT) referral pathway EAPT (n = 290), or DA (n = 198) pathway.

How Team Science Is Documented and Described in Published Family Medicine Research.

Purpose: Increasingly emphasized by leaders in family medicine and primary care research, team science is an approach to research that requires clear documentation for replicability. Here, we report the approach to documenting team science in 2 US family medicine research journals.

Methods: Our interdisciplinary team, composed of MDs and PhDs from family medicine and other disciplines, established a definition of the "team science" construct, which included the utilization of interdisciplinary partnerships and/or collaboration with community-based organizations.

Pilot Study on Resuscitation Volume's Effect on Perfusion and Inflammatory Cytokine Expression in Peri-Burn Skin: Implications for Burn Conversion.

Fluid resuscitation after thermal injury is paramount to avoid burn shock and restore organ perfusion. Both over- and under-resuscitation can lead to unintended consequences affecting patient outcomes. While many studies have examined systemic effects, limited data exist on how fluid resuscitation impacts burn wound progression in the acute period.

An Analysis of the Presence of Coagulopathy on Arrival to Emergency Departments in Deployed Military Treatment Facilities.

Introduction: Acute traumatic coagulopathy remains a high predictor of mortality; however, the incidence and outcomes in the military population have limited data. The goal of our study is to characterize coagulopathy on arrival to deployed Role 2 and Role 3 Military Treatment Facilities (MTFs).

Materials And Methods: We utilized the Department of Defense Trauma Registry to identify United States (U.