Deciphering protein-DNA interactions of with transcription factors through molecular docking, molecular dynamics simulations, and gene expression analysis.

Metastasis is a key hallmark of cancer aggressiveness, particularly in triple-negative breast cancer (TNBC), which lacks effective targeted therapies. Kisspeptin-1 (KISS1), a known metastasis suppressor is emerging as a potential therapeutic modulator. This study investigates the structural and regulatory interactions between and key transcription factors (TFs) involved in metastasis: SP1, CDX2, FLI1, GATA2, NMYC, and HDAC2.

In-vitro Analysis of Solanum virginianum L. Extract and Melatonin: Synergistic Induction of Apoptosis in MDA-MB-231 Breast Cancer Cells.

Solanum virginianum (Sv) herb finds widespread usage in various medical systems, notably it is a component of the traditional herbal formulation "Dashamula". Melatonin is widely acknowledged as a chemical and a cell defender owing to its anti-oxidative and immunomodulatory properties. Despite extensive references in traditional medicine, systematic evaluations of Solanum virginianum anticancer properties are scarce.

Different dietary compositions alter pubertal onset in Wistar rats.

Introduction: Puberty is a crucial developmental phase influenced by neuroendocrine signals involving various neuropeptides and hormones. The impact of various diet combinations on the initiation of puberty in female rats was studied, along with the crosstalk between hypothalamic neuropeptides that might influence it.

Methods: Weaned female Wistar rats were segregated into five groups and given different diets for a duration of 21 d.

Study on Synthesis, Antimicrobial Properties, Antioxidant Effects, and Anticancer Activity of New Quinoline Derivatives.

Quinolone molecule has demonstrated very high oral bioavailability and antimicrobial spectrum, which makes it a very strong candidate in the field of potency of drugs. In this study, a novel scaffold 6-amino-1-(4-methoxybenzyl) quinolin-2(1H)-one synthesized through a multistep reaction process. The antimicrobial activities demonstrate that the synthesized compounds 6a, 6b, 6d, 6m, and 6n show effective antimicrobial activities against bacterial (Escherichia coli, Serratia marcescens, Bacillus subtilis, and Staphylococcus aureus) and fungal strain (Saccharomyces cerevisiae).

Administration of Exogenous Kisspeptin Induces Breeding Competence in Post-pubertal Female Common Carp (Cyprinus carpio).

Puberty is a crucial stage in vertebrates, controlled by complex neuroendocrine mechanisms, yet its precise pathways remain unclear. Kisspeptin and melatonin are central to this regulation, acting as opposing forces: kisspeptin activates the hypothalamic-pituitary-gonadal (HPG) axis, while melatonin suppresses it. Serotonin also influences GnRH secretion.

Curcuminoid-Linked Lemon-Derived Carbon Dots for pH-Triggered Drug Release.

The supramolecular architectures were designed from lemon-derived carbon dots (LCDs) and curcuminoids. Curcuminoids were used to link the carbon dots to create a supramolecular architecture via an ester linkage. The optical characteristics of curcuminoid-linked lemon carbon dots (CL-LCDs) demonstrated that CL-LCDs-1 can be used for bioimaging applications among all of the LCD derivatives.

A comparative genotoxicity study of agrochemicals: nuclear abnormalities, comet assay, and gene expression alterations.

Agrochemicals (AGs) are known for their ability to have a negative impact on the health of non-target species, despite the fact that they are meant to protect agricultural plants from harmful pests. Catla catla (Hamilton, 1822) gill cells (ICG) were exposed to four AGs: insecticide (Imidacloprid (IMI)), fungicide (Curzate (CZ)), herbicide (pyrazosulfuron ethyl (PE)), and fertilizer micronutrients (MN) with sublethal concentrations 1/20th, 1/10th, and 1/5th of IC, described here as low dose (LD), medium dose (MD), and high dose (HD), respectively. A significant dose-dependent increase in the nuclear abnormalities such as micronuclei formation, bi-nucleated, and lobbed nucleated cells was observed in ICG cells treated with AGs.

Thermal and pressure response of KHg(CN)(SCN).

When aqueous solutions of KSCN and Hg(CN) were mixed, we observed the formation of long, needle-shaped single crystals with an unexpected structure and a formula of KHg(CN)(SCN). Single-crystal X-ray diffraction revealed that the CN ligands remain confined to the two apical positions of Hg(II) without forming an extended network. Instead, the sulphur atoms create a layered structure by forming four S-Hg bonds (3.

De novo Transcriptomic analysis to unveil the deltamethrin induced resistance mechanisms in Callosobruchus chinensis (L.).

The use of synthetic insecticides has been crucial in the management of insect pests however the extensive use of insecticides can result in the development of resistance. Callosobruchus chinensis is a highly destructive pest of stored grains, it's a major feeder and infests a range of stored grains that are vital to both global food security and human nutrition. We extensively investigated gene expression changes of adults in response to deltamethrin to decipher the mechanism behind the insecticide resistance.

Toxic effects of deltamethrin on oxidative stress, behavioural, organosomatic indices and histopathological changes in Digitonthophagus gazella (Coleoptera: Scarabaeinae).

Pyrethroids pose a great concern to the declining population of dung beetles and the sustainability of ecosystem services. This study aimed to investigate the toxic effects of deltamethrin on Digitonthophagus gazella. First, the LC value (0.

Cancer Stem Cell Regulation as a Target of Therapeutic Intervention: Insights into Breast, Cervical and Lung Cancer.

Cancer Stem Cells (CSCs) play an important role in the development, resistance, and recurrence of many malignancies. These subpopulations of tumor cells have the potential to self-renew, differentiate, and resist conventional therapy, highlighting their importance in cancer etiology. This review explores the regulatory mechanisms of CSCs in breast, cervical, and lung cancers, highlighting their plasticity, self-renewal, and differentiation capabilities.

Hormones as a double-edged sword: the role of hormones in cancer progression and the potential of targeted hormone therapies.

Cancer remains a significant cause of mortality in the world, with increasing prevalence worldwide. There are numerous treatments ranging from surgery to chemotherapy and radiotherapy, but since cancer is a heterogeneous disease, only few patients possibly respond to treatments. However, it opens a huge space for the advent of targeted therapies such as hormone therapy, immunotherapy, and target-specific drugs.

Identifying Needs and Preparing for Curriculum Changes in Indian Dental Education.

Unlabelled: The aims of dental professional courses are to prepare dental healthcare providers to manage common dental diseases, perform surgeries, and offer prevention. They should also be equipped to understand the needs of the society they are serving and willing to amend their skills. As they are expected to be a part of a team providing health care, they should have the requisite leadership and managerial skills for leading the team to serve its objectives in the best possible way.

Perceptions of Dental Teachers about Blended Learning: A Qualitative Analysis.

Objective: This qualitative study was conducted to explore the perceptions of dental teachers about utilizing blended learning (BL) in undergraduate teaching following the COVID-19 pandemic. The objectives were to study their willingness to move to BL and to identify the reasons for their willingness or lack thereof for this transition.

Materials And Methods: This descriptive qualitative study is part of doctoral research that was conducted among health sciences teachers from the state of Maharashtra, India.

Environmentally relevant concentrations of nickel and imidacloprid induce reproductive toxicity in earthworm (Eisenia fetida fetida).

The current research investigates individual and combined toxicity effects of nickel (Ni) and imidacloprid (IMI) on earthworm species Eisenia fetida fetida. Employing standardized toxicity parameters, we assessed the impact of environmentally relevant concentrations (ERC) of Ni, IMI, and their mixtures on key biomarkers and reproductive fitness of earthworms. Our findings reveal concentration-dependent responses with discernible adverse effects on physiological parameters.

Decoding the effect of photoperiodic cues in transducing kisspeptin-melatonin circuit during the pubertal onset in common carp.

This study unravels the intricate interplay between photoperiod, melatonin, and kisspeptin to orchestrate the pubertal onset of Common carp. Female fingerlings exposed to long days (LD) exhibited a hormonal crescendo, with upregulated hypothalamic-pituitary-ovarian (HPO) axis genes (kiss1, kiss1r, kiss2, gnrh2, gnrh3) and their downstream targets (lhr, fshr, ar1, esr1). However, the expression of the melatonin receptor (mtnr1a) diminished in LD, suggesting a potential inhibitory role.

Firm-created content on YouTube for product promotion - A thematic analysis for menstrual cup.

Mensuration cup is a mensural hygiene product about which people are less aware. Despite having many advantages there hasn't been much uptake. Research suggests that this is because of less accessible information.

Longitudinal in vivo cationic contrast-enhanced computed tomography classifies equine articular cartilage injury and repair.

Cationic contrast-enhanced computed tomography (CECT) capitalizes on increased contrast agent affinity to the charged proteoglycans in articular cartilage matrix to provide quantitative assessment of proteoglycan content with enhanced images. While high resolution microCT has demonstrated success, we investigate cationic CECT use in longitudinal in vivo imaging at clinical resolution. We hypothesize that repeated administration of CA4+ will have no adverse side effects or complications, and that sequential in vivo imaging assessments will distinguish articular cartilage repair tissue from early degenerative and healthy cartilage in critically sized chondral defects.

The Effectiveness of Virtual Reality in Controlling Pain and Anxiety Levels in Four-to-Six-Year-Old Children During Dental Treatment.

Background and objective Oral health is critical for children's overall well-being; however, dental anxiety often leads to the avoidance of necessary dental care among children. Virtual reality (VR) has emerged as a successful adjunctive tool in various clinical settings, including pain management. This cohort study explores the use of VR technology in reducing anxiety and pain perception during dental procedures for children.

Emerging role of microRNAs as regulators of protein kinase C substrate MARCKS and MARCKSL1 in cancer.

MicroRNAs (miRNAs) have emerged as pivotal regulators of gene expression, playing essential roles in diverse cellular processes, including the development and progression of cancer. Among the numerous proteins influenced by miRNAs, the MARCKS/MARCKSL1 protein, a key regulator of cellular cytoskeletal dynamics and membrane-cytosol communication, has garnered significant attention due to its multifaceted involvement in various cancer-related processes, including cell migration, invasion, metastasis, and drug resistance. Motivated by the encouraging early clinical success of peptides targeting MARCKS in several pathological conditions, this review article delves into the intricate interplay between miRNAs and the MARCKS protein in cancer.

Patent landscape highlighting therapeutic implications of peptides targeting myristoylated alanine-rich protein kinase-C substrate (MARCKS).

Introduction: MARCKS protein, a protein kinase C (PKC) substrate, is known to be at the intersection of several intracellular signaling pathways and plays a pivotal role in cellular physiology. Unlike PKC inhibitors, MARCKS-targeting drug (BIO-11006) has shown early success in clinical trials involving lung diseases. Recent research investigations have identified two MARCKS-targeting peptides which possess multifaceted implications against asthma, cancer, inflammation, and lung diseases.

Wogonin, as a potent anticancer compound: From chemistry to cellular interactions.

Chinese native medicine Georgi, also referred to as Chinese skullcap or Huang-Qin, is frequently used to treat cancer, viral infections, and seizures. This plant's abundance of flavones (wogonoside) and their related aglycones (wogonin) is responsible for many of its pharmacologic effects. A significant ingredient in that has been the subject of the most research is wogonin.

Repurposing Pitavastatin and L-Glutamine: Replenishing β-Cells in Hyperlipidemic Type 2 Diabetes Mouse Model.

Type 2 diabetes (T2D) is associated with obesity and declining β-cells. L-glutamine has been implicated in the amelioration of T2D by virtue of its incretin secretagogue property while, there are mixed reports on pitavastatin's adiponectin potentiating ability. We aimed to investigate the effect of pitavastatin (P), L-glutamine (LG), and combination (P + LG) on glycemic control and β-cell regeneration in a high-fat diet (HFD) + streptozotocin (STZ)-induced T2D mouse model.