Conditional Protein Structure Generation with Protpardelle-1c.

We present Protpardelle-1c, a collection of protein structure generative models with robust motif scaffolding and support for multi-chain complex generation under hotspot-conditioning. Enabling sidechain-conditioning to a backbone-only model increased Protpardelle-1c's MotifBench score from 4.97 to 28.

Dynamics of bacterial biofilm development imaged using light sheet fluorescence microscopy.

Biofilm formation exacerbates bacterial infections and interferes with industrial processes. However, the dynamics of biofilm development, especially if formed by a combination of more than one species, is not entirely understood. Here, we present a microfluidic cultivation system that enables continuous imaging of biofilm growth using light sheet fluorescence microscopy (LSFM).

Diversity of filariae circulating in South Caucasian bats and their ectoparasites.

Recent research on chiropteran parasites suggests a high prevalence and diversity, and extensive spatial distribution of filarial species; however, ecological and phylogenetic studies are still in their infancy. We sampled blood from 78 bat specimens, collected 1181 ectoparasites at summer colonies in Armenia and Georgia, and used nested-PCR targeting the cytochrome oxidase subunit 1 (1) gene to detect and genotype filarial parasites. The overall prevalence of filarial DNA was 17.

Cholesterol Derivatives Regulate Adenylyl Cyclase 7 Activity by Binding CARC and CRAC Motifs in the Cytosolic Subunits.

Transmembrane adenylyl cyclases (tmACs; ACs) are enzymes that synthesize cyclic AMP (cAMP), which is a key molecule in cellular signaling. Disruptions in AC activity can lead to long-term shifts in cAMP levels associated with various pathologies. In our study, we analyzed AC primary sequences and identified cholesterol-binding CARC and CRAC motifs located in conserved cytosolic regions, a surprising finding for motifs that are typically membrane-associated.

Proficiency in Esophageal Peroral Endoscopic Myotomy: A Meta-Analysis of Learning Curves.

Introduction: Our analysis examines the learning curve of peroral endoscopic myotomy (POEM) training using procedure duration as a key indicator of proficiency.

Methods: We searched PubMed, Embase, Cochrane, and Web of Science from inception until November 2024 for studies evaluating the learning curve of POEM. We divided the data into 0-20, 20-40, and 40-60 cumulative procedure phases of learning.

Sex Differences in the Association Between Physical Functioning and Cognition in Two Central European Populations.

Background: Evidence on the relationship between physical and cognitive functions remains inconsistent, and the role of sex differences is underexplored. This study examines the predictive value of a composite Physical Functioning Score (PFS) for cognitive function and assesses sex-specific associations in an Eastern European population.

Methods: Data from 7309 participants (mean age 59 ± 7.

Porphyrins as a Platform for Lithium-Selective Two-Dimensional Membranes.

Two-dimensional ion-selective membranes provide an appealing avenue for extracting lithium from naturally occurring aqueous brine solutions. Most current work is focused on graphene-based membranes, where the dynamics of ion permeation is determined by noncovalent pore-ion interactions, and the main challenge is achieving precise control over the pore size. Here, we propose that porphyrins, macrocycles with atomically precise pores ∼2.

Seasonal dynamics and potential interactions of haematophagous abomasal nematodes in two chamois populations in the Czech Republic.

Background: Pathogenic blood-feeding nematodes, such as Haemonchus contortus and the invasive Ashworthius sidemi, infect a wide range of wild and domestic ruminants. While the spread of A. sidemi among European cervids has been studied, its presence in chamois (Rupicapra rupicapra) remains poorly documented.

Fractional Flow Reserve-Guided Complete vs Culprit-Only Revascularization in Non-ST-Elevation Myocardial Infarction and Multivessel Disease: The SLIM Randomized Clinical Trial.

Importance: The benefits of fractional flow reserve (FFR)-guided complete coronary revascularization in patients with non-ST-segment elevation myocardial infarction (NSTEMI) and multivessel disease remain unclear.

Objective: To compare FFR-guided complete revascularization of nonculprit lesions vs culprit-only revascularization in patients with NSTEMI and multivessel disease.

Design, Setting, And Participants: This prospective, investigator-initiated, multicenter, international randomized clinical trial was conducted at 9 hospitals in Europe.

Live-cell imaging of mammary organoids using light sheet microscopy.

The mammary gland is a dynamic organ whose parenchyma undergoes major development during puberty and extensive remodeling with each estrous cycle. These processes can be modelled and investigated in vitro via 3D cell culture techniques that employ specialized extracellular matrices and appropriate growth factors. The resulting mammary organoid cultures faithfully represent the mammary gland with respect to cellular heterogeneity, cell-cell contacts, overall architecture as well as response to growth factor stimuli and are amendable to a variety of molecular methods as well as microscopy techniques.

Tropomyosin isoforms encoded by TPM2 control the actin-bundling activity of fascin-1.

Background: In many types of tumors, the expression patterns of actin-binding proteins -fascin-1 and various isoforms of tropomyosin - are altered. Fascin-1 is an actin-bundling protein that promotes cancer cell motility, whereas tropomyosin functions as a tumor and metastasis suppressor. However, the mechanisms by which tropomyosin isoforms regulate fascin-1 remain poorly understood.

Liquid chromatography mass spectrometry of segmented hair cortisol - A Bayesian retrospective window.

This study aimed to develop a robust methodology for quantifying cortisol decay in segmented human hair and to establish accurate retrospective estimates of initial cortisol levels. Two independent probabilistic approaches were employed: i) a Bayesian multilevel analysis across 37 individuals with 3-6 hair segments each and ii) a Bayesian repeated sampling approach in a single individual with 10 segments collected over eight months. All hair segments were analyzed for cortisol concentration using liquid chromatography-mass spectrometry with on-line solid phase extraction.

Phenotype Analysis in Two Families With Otopalatodigital Syndrome Spectrum Disorder Based on FLNA Gene Variants.

Otopalatodigital spectrum disorders (OPDSD), comprising otopalatodigital syndromes types 1 and 2 (OPD1, OPD2) and frontometaphyseal dysplasia (FMD), are rare X-linked disorders caused by FLNA gene variants, with phenotypes ranging from mild skeletal anomalies to severe multisystem malformations. We describe two unrelated cases: a 14-year-old male (P1, FMD) and an aborted fetus (P2, OPD2). Whole-exome sequencing identified hemizygous maternally inherited FLNA gene variants in P1 (c.

Effectiveness and Cost-Benefit Evaluation of Closed Incision Negative Pressure Therapy (ciNPT) in Patients After Major Revascularization Procedures.

IntroductionWound healing complications (WHC) of groin incisions are frequently discussed in vascular surgery. Various preventive measures are often applied to reduce their rates, but not all have the desired effect, and all these measures have their respective costs. The main aim of this study is to determine the effectiveness of closed incision negative pressure therapy (ciNPT) in reducing WHCs in high-risk patients after major revascularization procedures compared to the conventional adhesive dressing and to investigate the cost-benefit of its routine use.

Development of pyrazolo[1,5-a]pyrimidine based macrocyclic kinase inhibitors targeting AAK1.

Since the outbreak of SARS-CoV-2 in recent years, our society has become more aware that zoonotic diseases pose a real threat. Therefore, the demand for small molecules that target host proteins, essential for viral entry and replication, has increased as an interesting strategy for the development of antiviral agents, as these agents may be effective against several different pathogens. NAK kinases is one such potential target family because they are involved in a variety of cellular functions, hijacked by viruses to invade host cells, such as clathrin-mediated endocytosis.

Processes for Developing Plain Language Versions of Guidelines Recommendations: A Scoping Review.

Rationale: Plain language recommendations (PLRs) offer clear and understandable statements based on trustworthy guidelines.

Objective: We aimed to synthesise methodological evidence for developing PLRs in the health care context.

Methods: We followed the JBI methodology for scoping review development and the PRISMA-ScR guidelines for scoping review reporting.

Analysis of subsp. predicted outer membrane proteins (OMPeomes) in 21 clinical samples: variant sequences are predominantly surface-exposed.

The incidence of syphilis, a sexually transmitted disease caused by the subsp. (TPA), has been surging globally despite effective antibiotic therapy. A new strategy for syphilis control is the development of a multi-component syphilis vaccine with global efficacy, which requires the identification of surface-exposed candidate vaccinogens and the determination of their antigenic diversity within circulating TPA strains.

Onion thiolanes as multifunctional molecules: a story about recently discovered compounds from a well-known vegetable.

Onion ( L.) contains various bioactive organosulfur compounds, including recently identified species with a 3,4-dimethylthiolane ring structure. In this study, the biological activities of 24 onion-derived thiolane samples were comprehensively evaluated, focusing on antioxidant, anti-inflammatory, antidiabetic, and antimicrobial potential.

The Role of Sonication in Improving the Detection of Periprosthetic Joint Infections: a Prospective Analysis.

Purpose Of The Study: The diagnosis of periprosthetic joint infection (PJI) can be particularly challenging in cases of low-grade chronic infection. The suspicion of infection is typically confirmed through cultures of synovial fluid and periprosthetic tissue. However, these methods may not always detect low-grade infections, which can lead to persistent infection and early failure of the prosthesis.

Restoring cytonuclear harmony: Distinct strategies in Arabidopsis auto- and allopolyploids.

Plants rely on tight coordination between nuclear, mitochondrial, and chloroplast genomes to form essential multi-enzyme cytonuclear complexes. Whole-genome duplication (WGD) doubles the nuclear genome, potentially disrupting cytonuclear stoichiometry unless organellar genomes respond accordingly. Targeted analyses of chloroplasts and mitochondria enabled us to dissect the extent and mechanisms of adjustments in both organelles immediately after WGD and across generations in Arabidopsis auto- and allopolyploids.

Health Economic Impact of Incomplete Reperfusion Patterns After Endovascular Thrombectomy in Acute Ischemic Stroke.

Background And Purpose: Incomplete reperfusion in endovascular thrombectomy (EVT) impacts patients' outcomes. Different incomplete reperfusion patterns may benefit from targeted therapeutic strategies, e.g.

Reassessing the foundations of metric-affine gravity.

We reassess foundational aspects of Metric-Affine Gravity (MAG) in light of the Dressing Field Method, a tool allowing to systematically build gauge-invariant field variables. To get MAG started, one has to deal with the problem of "gauge translations". We first recall that Cartan geometry is the proper mathematical foundation for gauge theories of gravity, and that this problem never arises in that framework, which still allows to clarify the geometric status of gauge translations.

A new distinctive lineage of (Gastropoda, Stylommatophora, Helicidae), with a guide to species from mainland Greece.

Greece is home to numerous endemic land snail species, sometimes with highly restricted distributions. Several species of , representing all three subgenera, live there. Although the genus was taxonomically revised in 2014, there remained some open questions and the distribution ranges of individual species are still incompletely known.

Current Practices and Barriers of Family-focused Care of Patients with Severe Mental Illness and Their Children: A Survey Among Czech Psychologists and Psychiatrists.

Background: Children of parents with mental illness are known to be at risk of developing mental illnesses due to hereditary and socio-economic factors. The family-focused practice in the treatment of adult patients with severe mental illness benefits patients and their children, and can help prevent mental health problems in children of parents with mental illness. Therefore, professionals caring for adult patients must contribute to the early identification of families at risk and initiate the necessary care.

Antioxidant Power of Brown Algae: and Extracts Mitigate Oxidative Stress In Vitro and In Vivo.

Brown algae such as (AN) and (FV) are gaining considerable attention as functional feed additives due to their health-beneficial properties. This study evaluated the antioxidant potential of AN and FV extracts in intestinal epithelial cells and the in vivo model (). Aqueous AN and FV extracts were characterized for total phenolic content (TPC), antioxidant capacity (TEAC, FRAP), and phlorotannin composition using LC-HRMS/MS.