N-glycans in lung tissue specimens: a prospective target for enhanced cancer diagnosis and prognosis.

Background: It is well established that the cancerous transformation of cells is accompanied by profound alterations in glycosylation. In this study, we demonstrate the diagnostic potential of N-glycan profiling in tissue specimens from patients, primarily representing the two major types of lung cancer: non-small cell and small cell lung cancer.

Methods: Lung tissues and biopsies obtained from surgery and bronchoscopy underwent sample processing and enzymatic digestion.

The use of parenteral application of ferric carboxymaltose in the prevention of iron deficiency anaemia in suckling piglets.

This study aimed to evaluate the effectiveness of ferric carboxymaltose injection in the prevention of anaemia in suckling piglets. In group A ( = 8), piglets were administered iron in the form of iron carboxymaltose complex (Ferinject) at a dose of 200 mg i.m.

Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions.

This pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical silver and gold nanoparticles, which remained stable over an extended period, were employed as enhanced surfaces.

Efficacy of oral afoxolaner (NexGard®) against natural infestations with Heterodoxus spiniger (Phthiraptera: Boopiidae) in dogs under field conditions.

Heterodoxus spiniger is a chewing louse of a diverse range of hosts, primarily Carnivora. Domestic dogs are considered a major host, and infestations can lead to pruritus, hair loss, or seborrhea. In the context of absence of clinical trials evaluating the efficacy of isoxazolines against H.

Towards an international consensus on cross-border surrogacy: the role of the European Court of Human Rights?

The article examines ways to address the problems of cross-border surrogacy, based on existing initiatives to create an international legal framework and the case law of the European Court of Human Rights (ECtHR). I will first outline the problems associated with cross-border surrogacy and describe efforts to overcome these problems by adopting an international treaty or universally accepted standards in this area. Then I turn my attention to the nature of the right to private and family life and the general conditions for balancing this right against various public interests.

Determination of the radon progeny activity size distribution at different types of workplaces.

Inhalation of radon and its short-lived progeny is one of the most significant contributors to the total effective dose from natural sources of ionising radiation. Exposure to radon progeny represents a substantial health risk, primarily due to its established link to lung cancer. Dose coefficients are derived from biokinetic models describing the behaviour of radon decay products in the respiratory tract, combined with dosimetric models that account for energy deposition from emitted radiation.

Perceptions and Needs of Stakeholders Regarding MyPal Project's Electronic Patient-Reported Outcome App: Cross-Sectional Qualitative Focus Group Study.

Background: Palliative care is crucial for patients with life-threatening and serious diseases such as cancer, as it addresses their physical, psychosocial, and spiritual needs. Hematological malignancies significantly contribute to global cancer cases, impacting both older adults and children. To meet the increasing demand for palliative care, electronic patient-reported outcome (ePRO) interventions offer valuable insights into patient monitoring and treatment decision-making.

Sequence variability of BamA and FadL candidate vaccinogens suggests divergent evolutionary paths of outer membrane proteins.

Knowledge of subspecies () outer membrane protein (OMP) sequence variability is essential for understanding spirochete proliferation within endemic populations as well as the design of a globally effective syphilis vaccine. Our group has identified extracellular loops (ECLs) of BamA (TP0326) and members of the FadL family (TP0548, TP0856, TP0858, TP0859, and TP0865) as potential components of a multivalent vaccine cocktail. As part of a consortium to explore strain diversity, we mapped the variability of BamA and FadL orthologs in 186 strains from Malawi, China, and Colombia onto predicted 3D structures.

Body size and intracranial volume interact with the structure of the central nervous system: A multi-center in vivo neuroimaging study.

Clinical research emphasizes the implementation of rigorous and reproducible study designs that rely on between-group matching or controlling for sources of biological variation such as subject's sex and age. However, corrections for body size (i.e.

Construction of a syntrophic consortium with reciprocal substrate processing.

Synthetic microbial consortia can leverage their expanded enzymatic reach to tackle biotechnological challenges too complex for single strains, such as biosynthesis of complex secondary metabolites or waste plant biomass degradation and valorisation. The benefit of metabolic cooperation comes with a catch-installing stable interactions between consortium members. Here, we established a mutualistic relationship in the synthetic consortium of strains through reciprocal processing of two disaccharides-cellobiose and xylobiose-obtainable from lignocellulosic residues.

Irreversible furin cleavage site exposure renders immature tick-borne flaviviruses fully infectious.

Flavivirus assembly is driven by the envelope glycoproteins pre-membrane (prM) and envelope (E) in the neutral pH environment of the endoplasmic reticulum. Newly budded, spiky particles are exported through the Golgi apparatus, where mildly acidic pH induces a major surface rearrangement. The glycoproteins reorganize into (prM/E)\₂ complexes at the surface of smooth particles, with prM trapped at the E dimer interface, thereby exposing a furin cleavage site (FCS) for proteolytic maturation into infectious virions.

Reticulate allopolyploidy and subsequent dysploidy drive evolution and diversification in the cotton family.

Polyploidy and subsequent post-polyploid diploidization (PPD) are key drivers of plant genome evolution, yet their contributions to evolutionary success remain debated. Here, we analyze the Malvaceae family as an exemplary system for elucidating the evolutionary role of polyploidy and PPD in angiosperms, leveraging 11 high-quality chromosome-scale genomes from all nine subfamilies, including newly sequenced, near telomere-to-telomere assemblies from four of these subfamilies. Our findings reveal a complex reticulate paleoallopolyploidy history early in the diversification of the Malvadendrina clade, characterized by multiple rounds of species radiation punctuated by ancient allotetraploidization (Mal-β) and allodecaploidization (Mal-α) events around the Cretaceous-Paleogene (K-Pg) boundary.

Association of residual shunts after PFO closure with recurrent stroke risk: Single-center experience.

Background: Literature on residual right-to-left shunts after patent foramen ovale (PFO) closure shows conflicting results regarding stroke recurrence risk, though recent data suggest increased risk with moderate and large residual shunts.

Aims: To evaluate the effect of the presence and grade of residual shunt on the risk of stroke/transient ischemic attack (TIA) after PFO closure.

Material And Methods: We retrospectively analyzed data from a prospectively maintained registry of 498 patients who underwent PFO closure following cryptogenic stroke/TIA between 2004-2022.

Efficacy of second- and third-line chemotherapy after chemotherapy with platinum doublet and immunotherapy in non-small cell lung cancer: a descriptive study.

Background: The efficacy of second-line chemotherapy with pemetrexed or docetaxel was evaluated in phase III clinical trials only after prior treatment with a platinum doublet in non-small cell lung cancer. The effectiveness of second-line chemotherapy after previous chemotherapy and immunotherapy is not well known. The effectiveness of third-line chemotherapy is not practically proven in this sense.

Stereotactic radiosurgery targeting the stellate ganglion for refractory ventricular arrhythmias: A first-in-human case report with 18-month follow-up.

Introduction: We present the first-in-human application of stereotactic radiosurgery (SRS) targeting the left stellate ganglion (LSG) as a non-invasive neuromodulatory strategy for treating polymorphic ventricular tachycardia/ventricular fibrillation (VT/VF).

Materials And Methods: A 69-year-old man with mixed-etiology cardiomyopathy (LVEF 28 %) experienced 27 VT/VF episodes over three months. This resulted in 18 implantable cardioverter-defibrillator (ICD) shocks and nine anti-tachycardia pacing therapies, despite optimal pharmacotherapy, catheter ablation and two temporary LSG blocks.

Is There Only One Well-Being for Younger Children and Preadolescents? The Revision of the SCWBS Questionnaire with Detailed Psychometric Properties.

This study evaluates the assessment of the well-being of children and preadolescents by examining the psychometric properties of the Stirling Children's Well-Being Scale (SCWBS). Given the growing concerns about children's mental health, accurate and easily administrable measurement tools are urgently needed. This research represents the first in-depth investigation of the SCWBS's factor structure and psychometric characteristics in the largest sample of children under 15 years using multidimensional item response theory.

Flexibility-Aided Orientational Self-Sorting and Transformations of Bioactive Homochiral Cuboctahedron PdL.

The rational design and selective self-assembly of flexible and unsymmetric ligands into large coordination complexes is an eminent challenge in supramolecular coordination chemistry. Here, we present the coordination-driven self-assembly of natural ursodeoxycholic-bile-acid-derived unsymmetric tris-pyridyl ligand (L) resulting in the selective and switchable formation of chiral stellated PdL and PdL cages. The selectivity of the cage originates in the adaptivity and flexibility of the arms of the ligand bearing pyridyl moieties.

Reassessment of mechanical restitution in guinea pig cardiomyocytes through refined computational modelling.

Mechanical restitution (MR) represents the time recovery of the heart muscle's ability to contract. Despite intensive research, some aspects of MR remain unclear. To describe MR in guinea pig cardiac muscle, we modified our published mathematical model of guinea pig ventricular cardiomyocyte and supplemented it with a description of cellular contraction.

Efficacy and safety of ebopiprant to delay preterm birth after oral administration in pregnant women with spontaneous preterm labor receiving atosiban: a phase 2a, double-blind, parallel group, randomized, placebo-controlled, proof of concept study.

Objective: To assess the efficacy and safety of ebopiprant (OBE022), a novel, oral and selective prostaglandin F2α (PGF2α) receptor antagonist, to delay spontaneous preterm labor.

Methods: This randomized, placebo-controlled, double-blind study (NCT03369262) was conducted between January 2019 and March 2020 in large referral maternity hospitals across Czech Republic, Finland, Israel, Russia, Spain, and Vietnam. Enrolled participants had spontaneous preterm labor at a gestational age of 24-34 weeks, with ≥4 contractions/30 min, cervical dilatation of 1-4 cm, and ≥1 other sign of preterm labor (cervical length ≤25 mm, progressive cervical change, or a positive test [insulin-like growth factor-binding protein-1 or fetal fibronectin] for preterm labor).

Pediatric thalamic incidentalomas: a retrospective analysis of their characteristics, evolution, management, and prognostic factors for progression.

Purpose: The increasing availability of advanced neuroimaging has led to a rise in incidental findings among pediatric patients. Management strategies include immediate surgical intervention, observation or surgery upon progression. These are influenced by imaging characteristics, lesion behavior over time, patient/family preferences, and the lesion's surgical risks.

Anticoronavirus Activity of Uridine Glycoconjugates Containing a 1,2,3-Triazole Moiety.

Coronaviruses can spread rapidly to new host species and cause severe respiratory and enteric diseases in vertebrates, including humans. To date, seven coronaviruses have been identified in humans, with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) being the most notorious due to its substantial social and economic impact. Although anti-SARS-CoV-2 vaccines are available, infections remain widespread, highlighting the ongoing need for antiviral treatments.

Mental and physical health burden and quality of life in Czech women with lipedema.

Background: Lipedema is a chronic condition characterized by excessive fat deposition in the hips, buttocks, and lower legs, significantly impacting quality of life. In Czechia, limited research exists on the relationship between lipedema symptoms and depressive symptoms, despite the condition's prevalence and its impact on mental health.

Aim: This study aims to investigate the relationship between lipedema symptoms and the severity of depressive symptoms among Czech women and assess their quality of life.