Laser Interstitial Thermal Therapy (LITT) in pediatric neurosurgery: Single center retrospective analysis of 41 consecutive procedures.

Introduction: Laser Interstitial Thermal Therapy under MRI control has emerged as a safe and efficient alternative to microsurgery in epilepsy and neurooncology procedures. Yet it has been used only recently in seldom European centers. Here, we report our 4 years' experience with LITT in children (complications, epileptic and oncologic outcomes).

Comparing sexual outcomes and complications in Male-to-Female Gender-Affirming Surgery: penile inversion vs. robotic peritoneal vaginoplasty.

Gender dysphoria is a condition characterized by distress due to a mismatch between a person's gender identity and their assigned gender at birth. This study aimed to compare sexual satisfaction and complication rates in patients undergoing feminizing gender affirming surgery (fem-GAS) using two techniques: standard penile inversion vaginoplasty (PIV) and robotic peritoneal vaginoplasty (RPGAV). We conducted a retrospective analysis data from a prospective registry (2017-2022).

AI-driven and Traditional Radiomic Model for Predicting Muscle Invasion in Bladder Cancer via Multi-parametric Imaging: A Systematic Review and Meta-analysis.

Rationale And Objectives: This study systematically evaluates the diagnostic performance of artificial intelligence (AI)-driven and conventional radiomics models in detecting muscle-invasive bladder cancer (MIBC) through meta-analytical approaches. Furthermore, it investigates their potential synergistic value with the Vesical Imaging-Reporting and Data System (VI-RADS) and assesses clinical translation prospects.

Methods: This study adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines.

Design, synthesis and biological evaluation of 3,3-dimethyl-2,3,4,9-tetrahydro-1H-carbazole derivatives as AcrB inhibitors with potent antibiofilm effect for reversing bacterial multidrug resistance.

A series of novel 3,3-dimethyl-2,3,4,9-tetrahydro-1H-carbazole derivatives were rationally designed, synthesized and evaluated for their biological activity as AcrB inhibitors. The compounds were assessed for their antibiotic potentiating effects, followed by evaluation of Nile Red efflux inhibition, and off-target effects including activity on the outer and inner bacterial membranes. Ten compounds potentiated antibiotic activity at sub-inhibitory concentrations, reducing the minimum inhibitory concentrations (MICs) of at least one of the tested antibiotics by at least 8-fold, with three derivatives (7c, 11g, and 11i) achieving 32-fold MIC reductions at 128 μg/mL.

24-h urine test application in patients with kidney stone disease: a population-based study in a primary care setting.

Background: Kidney stone formation is driven by an imbalance between lithogenic substances and crystallization inhibitors. Current guidelines recommend a 24-h urine collection in patients with kidney stone disease to assess the risk of stone formation and monitor therapy compliance. However, real-world data on adherence to these guidelines remain limited and outdated.

Synthesis and biological evaluation of N-O heterobicyclic ring systems derived from 1,2-dihydropyridines as novel TRPA1 activators: implication in GLP-1 release and neuroprotection.

A set of small molecules containing an unusual sp-rich heterobicyclic scaffold were prepared and evaluated in vitro for their ability to increase GLP-1 secretion in STC-1 cells and to protect SH-SY5Y cells from acute and chronic damage induced by glucose and methylglyoxal, respectively. The results obtained showed that some compounds, especially those containing an electron-withdrawing and/or lipophilic group at the meta position of the aryl moiety present at position 9, are effective non-covalent TRPA1 agonists. The lead compound so far individuated (compound 4b) was also prepared by asymmetric synthesis in both enantiomeric forms.

Automated Kidney Tumor Segmentation in CT Images Using Deep Learning: A Multi-Stage Approach.

Rationale And Objectives: Computed tomography (CT) remains the primary modality for assessing renal tumors; however, tumor identification and segmentation rely heavily on manual interpretation by clinicians, which is time-consuming and subject to inter-observer variability. The heterogeneity of tumor appearance and indistinct margins further complicate accurate delineation, impacting histopathological classification, treatment planning, and prognostic assessment. There is a pressing clinical need for an automated segmentation tool to enhance diagnostic workflows and support clinical decision-making with results that are reliable, accurate, and reproducible.

Multitargeting neuroimmune pathways: novel candidates for chronic pain relief.

Chronic pain remains inadequately managed, partly because of insufficient consideration of neuroimmune interactions in therapeutic design and a continued reliance on single-target strategies ill-suited to its complexity. Multitarget-directed ligands that modulate the non-neuronal microenvironment of neuronal pain pathways show promise, supported by encouraging preclinical data and initial clinical findings.

External validation of the revised lethal triad criteria for appropriate strategic decision making.

Background: The conventional lethal triad criteria for trauma are too specific for death and, thus, inappropriate as a standard for strategic decisions. Revised lethal triad criteria were previously proposed based on a multicenter study. Positive is defined as meeting one major criterion (fibrin/fibrinogen degradation product >90 μg/mL) or two minor criteria (base excess <-3 mEq/L or temperature <36°C).

Decidual/placental and first trimester plasma levels of hsa-miR-199a-3p|hsa-miR-199b-3p and hsa-miR-3150b-3p are associated with insulin secretion in pregnancy.

Background/aims: The placenta expresses and releases specific microRNAs (miRNAs) into the maternal circulation that may influence insulin secretion during pregnancy. We hypothesized that specific decidual/placental miRNAs are associated with maternal insulin secretion during pregnancy.

Methods: In the Genetics of Glucose regulation in Gestation and Growth (Gen3G) prospective cohort, we estimated maternal insulin secretion using the Stumvoll first phase index derived from an oral glucose tolerance test at ~26 weeks of gestation.

Perinephric Myxoid Pseudotumor of Fat: A Series of 29 Cases Demonstrating Inconsistent Associations With Underlying Kidney Disease.

Perinephric myxoid pseudotumor of fat (PMPTF) is a recently characterized lesion typically associated with non-neoplastic renal disease. Its pathogenesis is thought to result from chronic renal "irritation," either due to mass effect from renal carcinoma or inflammation related to benign renal conditions. Prompted by several cases arising in the absence of underlying renal pathology, we conducted a multi-institutional study of 29 mass-forming cases with detailed clinical, histologic, and molecular characterization.

Prevalence of sacroiliitis on magnetic resonance enterography in Crohn's disease and its association with intestinal findings: a monocentric observational cross-sectional study.

Magnetic resonance enterography (MRE) is recommended for the assessment of small intestine alterations in Crohn's disease (CD). Sacroiliac joints (SIJs) imaging has a central role in the early diagnosis of sacroiliitis (SI). MRE can evaluate both acute and structural findings of SIJs.

HNF1A-inactived Hepatocellular Adenomas in Fibropolycystic Kidney and Liver Disease Are Associated with Germline HNF1B Variant.

HNF1A-inactivated hepatocellular adenomas are rarely described in patients with hepatorenal fibrocystic disease, and their genetic relationship remains unexplored. This study reports a series of HNF1A-inactivated hepatocellular adenomas with molecular sequencing analysis in patients with fibrocystic kidney and/or liver disease. Cases were retrospectively identified from the pathology archives of three institutions.

ENDOCOR: a nationwide consortium of endocarditis teams-initiating a registry for infective endocarditis within the Netherlands Heart Registration.

Background: Despite advancements in diagnostics and treatment strategies, infective endocarditis continues to carry a substantial morbidity and mortality risk. In addition, the field of infective endocarditis contains many gaps in evidence, as international guidelines are predominantly based on low-level evidence. To improve infective endocarditis care and survival rates in the Netherlands, adequate evaluation of diagnostics, treatment strategies and outcomes is essential.

The impact of autoimmune comorbidities on multiple sclerosis progression: insights from a longitudinal single-center study.

Multiple sclerosis (MS) is a chronic autoimmune disease that affects the central nervous system. The presence of other conditions alongside MS imposes a substantial personal and socioeconomic burden. In particular, the impact of comorbid autoimmune diseases (AID) on MS outcomes remains uncertain.

Length-based stock assessment and population dynamics of two flat fishes, in relation to environmental parameters from Hooghly estuary (Bay of Bengal), India: implication for sustainable resource management.

The present research focused on a detailed stock assessment of Cynoglossus cynoglossus and Brachirus orientalis in the Hooghly estuary from January to December 2023. Length-weight data from 1601 specimens of C. cynoglossus and 550 of B.

2D Speckle Tracking Strain Echocardiography in Multisystem Inflammatory Syndrome in Children: A Multicenter Analysis From the MUSIC Study.

Background: 2D-speckle tracking echocardiography may help detect subclinical ventricular dysfunction, but data in multisystem inflammatory syndrome in children (MIS-C) are scarce. We investigated left ventricular (LV) strain parameters in MIS-C and their association with outcomes.

Methods: We performed an ambi-directional, 32-center cohort study on hospitalized MIS-C patients (March 2020-November 2021) with at least 1 echocardiogram read by the Core Lab.

Atrial Mechanics in Heart Failure With Preserved Ejection Fraction: Effect of a No-Implant Interatrial Shunt.

Background: The atria play an important role in the pathophysiology of heart failure with preserved ejection fraction. Decreased left atrial strain is associated with worse clinical outcomes. The impact of no-implant interatrial shunting on atrial structure and function has not been described.

[Effectiveness of "brain-computer" interfaces with biofeedback in the rehabilitation of cognitive impairment after a stroke].

Objective: Comparison of the effectiveness of two "brain-computer" interface (BCI) software complexes using biofeedback (BF) and standard therapy in restoring cognitive functions after a stroke.

Material And Methods: Eighty-nine stroke patients were examined. Neuropsychological testing was carried out using the Montreal Cognitive Assessment Scale (MoCA), the Tracking test, the Wechsler subtest 9 Kohs block design test, the Schulte tables, the Memorization of 10 Words test (according to A.

Modeling Genetic Risk of β-Cell Dysfunction in Human Induced Pluripotent Stem Cells From Patients Carrying the MTNR1B Risk Variant.

Disruptions in circadian rhythm, partly controlled by the hormone melatonin, increase the risk of type 2 diabetes (T2D). Accordingly, a variant of the gene encoding the melatonin receptor 1B (MTNR1B) is robustly associated with increased risk of T2D. This single-nucleotide polymorphism (SNP; rs10830963; G-allele) is an expression quantitative trait locus (eQTL) in human pancreatic islets, conferring increased expression of MTNR1B, which is thought to perturb pancreatic β-cell function.

A multidisciplinary emergency protocol reduces revascularization time in major upper and lower limb replantations.

Background: Major limb amputation salvage procedures exhibit an increased risk of failure when revascularization is delayed beyond 360 min. Institutional delays persist as critical barriers, even with advancements in surgical techniques.

Methods: Retrospective cohort study (November 2022- December 2024) at Level I Trauma Center.

DNA deformability defines sequence-dependent capture of gyrase.

Bacterial gyrase, unique among type II topoisomerases, introduces negative supercoils into DNA. Mechanistic details of gyrase still must be elucidated because of the complexity of the process and the difficulty in visualizing it. Specifically, the interplay among base sequence, local DNA deformability, and global DNA topology for gyrase site selection is unclear.

FAP-CAR-T cells reduce dystrophic muscle fibrosis, improving adeno-associated virus gene transfer efficacy.

Tissue fibrosis is a pathological feature of many diseases including muscular dystrophies such as Duchenne muscular dystrophy (DMD). Fibrosis may limit the effectiveness of gene therapy in muscle impacting on viral dosing but direct evidence is lacking. Strategies to reduce skeletal muscle fibrosis are limited.

Treatment patterns in women with breast cancer and endocrine therapy-related menopausal symptoms: a cohort study from the United States, United Kingdom, and Germany.

Objective: To describe treatment patterns for menopausal symptoms in women taking endocrine therapy for breast cancer treatment/prevention from the United States (US), United Kingdom (UK), and Germany.

Methods: We undertook a retrospective cohort study using data from the US Market Scan Commercial Claims and Encounters Data database, and electronic health records from the UK's Clinical Practice Research Database Aurum and the German Disease Analyzer. Women aged 18-65 years with a first prescription/dispensation for endocrine therapy for breast cancer treatment or prevention (index date) from 2010 to 2022 were followed up, and the following treatment classes were evaluated: antidepressants, benzodiazepines, anticonvulsants, antihypertensives, and hormone therapy.