Interdisciplinary management of paediatric chronic and complex pain: the experience of a specialised outpatient clinic in Italy.

Purpose: This study aimed to describe the structure, patient characteristics, and preliminary clinical outcomes of a dedicated interdisciplinary outpatient clinic for paediatric chronic and complex pain in Italy, with a focus on the feasibility of implementing a biopsychosocial care model.

Methods: We conducted a retrospective review of all patients referred to the Paediatric Specialised Pain Clinic of the University of Padua between January 2023 and May 2024. Data on demographics, clinical diagnoses, pain characteristics, treatments, and follow-up outcomes were collected.

Sustainable and healthy diet index (SHDI) unveils regional differences in Europe and Northern Africa: findings from the SysOrg study.

Purpose: We developed a diet quality index based on the Planetary Health Diet (PHD) to assess healthy and sustainable diets. The index was applied alongside socio-demographic characteristics in five regions across Europe and North Africa.

Methods: The Sustainable Healthy Diet Index (SHDI) was designed using existing and validated healthy diet indexes.

Grid cells accurately track movement during path integration-based navigation despite switching reference frames.

Grid cells, with their periodic firing fields, are fundamental units in neural networks that perform path integration. It is widely assumed that grid cells encode movement in a single, global reference frame. In this study, by recording grid cell activity in mice performing a self-motion-based navigation task, we discovered that grid cells did not have a stable grid pattern during the task.

Redox-driven mineral and organic associations in Jezero Crater, Mars.

The Perseverance rover has explored and sampled igneous and sedimentary rocks within Jezero Crater to characterize early Martian geological processes and habitability and search for potential biosignatures. Upon entering Neretva Vallis, on Jezero Crater's western edge, Perseverance investigated distinctive mudstone and conglomerate outcrops of the Bright Angel formation. Here we report a detailed geological, petrographic and geochemical survey of these rocks and show that organic-carbon-bearing mudstones in the Bright Angel formation contain submillimetre-scale nodules and millimetre-scale reaction fronts enriched in ferrous iron phosphate and sulfide minerals, likely vivianite and greigite, respectively.

DNA methylation cooperates with genomic alterations during non-small cell lung cancer evolution.

Aberrant DNA methylation has been described in nearly all human cancers, yet its interplay with genomic alterations during tumor evolution is poorly understood. To explore this, we performed reduced representation bisulfite sequencing on 217 tumor and matched normal regions from 59 patients with non-small cell lung cancer from the TRACERx study to deconvolve tumor methylation. We developed two metrics for integrative evolutionary analysis with DNA and RNA sequencing data.

On the origin of the late-flowering ppd-H1 allele in barley.

To breed for climate resilient crops, an understanding of the genetic and environmental factors influencing adaptation is critical. Barley provides a model species to study adaptation to climate change. Here we present a detailed analysis of genetic variation at a major photoperiod response locus and relate this to the domestication history and dispersal of barley.

Italian guidelines for cervical cancer screening. Multisocietal recommendations on the use of biomarkers in HPV screening with risk-based approach and GRADE methodology.

The European Council recommends adopting risk-based screening when relevant. In triaging HPV-positive women, it can be an effective strategy to reduce overtreatment and referral to colposcopy. HPV genotyping and p16/ki67 expression may allow a better risk stratification than cytology.

Improved GVHD-free relapse-free survival when rATG/ATLG is used in allo-HCT from matched sibling donors - an EBMT registry study by the Transplant Complications Working Party.

The EBMT recommends rabbit anti-thymocyte or anti-T-lymphocyte globulin (rATG/ATLG) as GVHD prophylaxis in matched sibling donor (MSD) allogeneic hematopoietic cell transplantation (allo-HCT). However, discrepancies between recommendations and clinical practice were reflected in the EBMT survey. Therefore, we performed retrospective EBMT registry analysis from 2014 to 2021 to reinforce the real-world evidence context of rATG/ATLG impact on post-transplantation outcomes.

Low-grade non-muscle-invasive bladder cancer: molecular landscape, treatment strategies and emerging therapies.

Low-grade non-muscle invasive bladder cancer is a specific category of bladder cancer with a favourable prognosis; however, its management presents several challenges. The risk of stage progression is very low, but approximately half of patients will experience recurrence within the first 5 years after diagnosis. This high propensity for recurrence, coupled with the threat of progression, mandates ongoing surveillance.

ABCA7 variants impact phosphatidylcholine and mitochondria in neurons.

Loss-of-function variants in the lipid transporter ABCA7 substantially increase the risk of Alzheimer's disease, yet how they impact cellular states to drive disease remains unclear. Here, using single-nucleus RNA-sequencing analysis of human brain samples, we identified widespread gene expression changes across multiple neural cell types associated with rare ABCA7 loss-of-function variants. Excitatory neurons, which expressed the highest levels of ABCA7, showed disrupted lipid metabolism, mitochondrial function, DNA repair and synaptic signalling pathways.

PML::RARα+ myeloid cells display metabolic alterations that can be targeted to treat resistant/relapse acute promyelocytic leukemias.

At present there is no metabolic characterization of acute promyelocytic leukemia (APL). Pathognomonic of APL, PML::RARα fusion protein rewires metabolic pathways to feed anabolic tumor cell's growth. All-trans retinoic acid (ATRA) and arsenic trioxide (ATO)-based therapies render APL the most curable subtype of AML, yet approximately 1% of cases are resistant and 5% relapse.

Chromosome-level assembly of the 400-year-old Goethe's Palm (Chamaerops humilis L.).

The rapid decline in global biodiversity highlights the urgent need for conservation efforts, with botanical gardens playing a crucial role in ex situ plant preservation. Monumental plants, such as the 400-year-old Goethe's Palm (Chamaerops humilis L.) at the Padua Botanical Garden serve as vital flagship species with significant ecological and cultural value.

Capturing CO under Dry and Humid Conditions: When Does the Parent MOF Outperform the MTV MOF?

A key challenge in capturing CO from postcombustion gases is humidity due to competitive adsorption between CO and HO. Multivariate (MTV) metal-organic frameworks (MOFs) have been considered a promising option to address this problem, e.g.

Systematic video analysis of ACL tear patterns in Spanish professional female football players: Neurocognitive errors as targets for prevention.

Objectives: This study aimed to analyse the mechanisms, injury patterns, biomechanics and neurocognitive factors of anterior cruciate ligament tears in professional female Spanish football players during training and competitive matches.

Design: Systematic video-analysis observational study.

Methods: Four hundred and sixty-one players from 16 teams of the Spanish top division (Liga F) were tracked over three consecutive seasons (2021/2022 to 2023/2024).

Association of intraoperative end-tidal CO levels with postoperative outcomes: a patient-level analysis of two randomised clinical trials.

Background: The relationship between intraoperative end-tidal CO (etCO) levels and postoperative outcomes remains unclear. We conducted a post hoc analysis of two randomised trials in adults undergoing major surgery under general anaesthesia.

Methods: We re-analysed individual participant data comparing high or low positive end-expiratory pressure with low tidal volume intraoperative ventilation using a merged database derived from two randomised trials in non-obese (PROVHILO: ISRCTN70332574) and obese (PROBESE: NCT02148692) patients.

Complex cervicocephalic artery dissections with wide intimal inlet: determinants and implications for endovascular recanalization.

Background And Purpose: Cervicocephalic artery dissection (CCAD) is a well-recognized cause of ischemic stroke. However, complex forms of CCAD, characterized by a wide intimal inlet without a visible intramural hematoma, pose diagnostic challenges and complicate endovascular access to the true lumen when recanalization is required. We aimed to analyze the clinical presentation, outcomes, and feasibility of endovascular treatment of complex CCAD and to propose a novel morphological classification.

Mouse intestine as a useful model for CFTR electrophysiology function analysis.

Cystic fibrosis (CF) is a genetic disorder primarily known for its severe impact on lung function, but it also significantly affects the digestive system, leading to complications such as intestinal blockages, malabsorption, inflammation, and microbial dysbiosis. The study of CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) effects on intestinal physiology is critical for developing new effective treatments. This work highlights the use of the mouse intestine as a valuable model for analyzing cellular electrophysiology and CFTR function.

Orthotopic model of tongue cancer for the study of head and neck squamous cell carcinoma in mice.

Head and neck squamous cell carcinoma (HNSCC) is among the most prevalent human malignancies globally, with approximately 887,000 new cases diagnosed each year. Currently, the standard treatment for HNSCC involves surgery, followed by radiotherapy, chemotherapy and immunotherapy. However, despite these available treatments, the survival rate of patients with HNSCC remains low.

Safety climate, safety behaviors, and workplace accidents: What are the mechanisms? A multi-level lagged study.

Introduction: Occupational safety is managed by multiple stakeholders at different levels within organizations. Leveraging theory and insights from previous occupational health and safety research, this study formulated and tested hypotheses concerning the interplay between safety climates generated by a team's supervisor and coworkers, workers' individual psychological processes (i.e.