Chemoenzymatic Total Synthesis of Lansai B.

Lansai B is an indole alkaloid whose biosynthesis is not yet elucidated. However, utilizing nature's tools enabled the stereoselective formation of this unique framework, first by providing the substituted tryptophan as starting material and later in the scaffold-building step forming the pyrroloindole motif. For the latter, the C3-methyltransferase from Streptomyces griseoviridis (SgMT) is employed for the diastereoselective methylation.

TopEC: prediction of Enzyme Commission classes by 3D graph neural networks and localized 3D protein descriptor.

Tools available for inferring enzyme function from general sequence, fold, or evolutionary information are generally successful. However, they can lead to misclassification if a deviation in local structural features influences the function. Here, we present TopEC, a 3D graph neural network based on a localized 3D descriptor to learn chemical reactions of enzymes from enzyme structures and predict Enzyme Commission (EC) classes.

Magnetizing Biotech-Advances in (In Vivo) Magnetic Enzyme Immobilization.

Industrial biocatalysis, a multibillion dollar industry, relies on the selectivity and efficacy of enzymes for efficient chemical transformations. However, enzymes, evolutionary adapted to mild biological conditions, often struggle in industrial processes that require harsh reaction conditions, resulting in reduced stability and activity. Enzyme immobilization, which addresses challenges such as enzyme reuse and stability, has therefore become a vital strategy for improving enzyme use in industrial applications.

Exploring biotechnology for plastic recycling, degradation and upcycling for a sustainable future.

The persistent demand for plastic commodities, inadequate recycling infrastructure, and pervasive environmental contamination due to plastic waste present a formidable global challenge. Recycling, degradation and upcycling are the three most important ways to solve the problem of plastic pollution. Sequential enzymatic and microbial degradation of mechanically and chemically pre-treated plastic waste can be orchestrated, followed by microbial conversion into value-added chemicals and polymers through mixed culture systems.

Untrained perceptual loss for image denoising of line-like structures in MR images.

In the acquisition of Magnetic Resonance (MR) images shorter scan times lead to higher image noise. Therefore, automatic image denoising using deep learning methods is of high interest. In this work, we concentrate on image denoising of MR images containing line-like structures such as roots or vessels.

Promoting Circular Economy by Leveraging Annatto Byproducts from L. into Sustainable Antioxidant Food Packaging.

Annatto ( L.) is cultivated primarily for the extraction of bixin, a natural dye with substantial industrial importance, resulting in the generation of large quantities of residues that remain underutilized. This study provides the first in-depth characterization of annatto byproducts derived through molecular distillation, highlighting their untapped potential for sustainable innovation.

Functional and Bioactive Benefits of Selected Microalgal Hydrolysates Assessed In Silico and In Vitro.

BIOPEP-UWM, a peptide database, contains 5128 peptides from a myriad of resources. Five listed peptides are Angiotensin-I-converting enzyme (ACE-1; EC3.4.

SIGHT-A System for Solvent-Tight Incubation and Growth Monitoring in High Throughput.

Physiological characterization of microorganisms in the context of solvent tolerance is a tedious process with a high investment of manual labor while often being limited in throughput capability simultaneously. Therefore, we developed a small-scale solvent-impervious cultivation system consisting of screw cap-sealed glass vials in combination with a 3D-printed vial holder for the Growth Profiler (EnzyScreen) platform. Components and cultivation conditions were empirically tested, and a suitable setup was found for the intended application.

Rapid exometabolome footprinting combined with multivariate statistics: A powerful tool for bioprocess optimization.

is used as an industrial platform organism for amino acid production. Previously, the organism was utilized to produce l-histidine with research focusing on metabolic engineering approaches to increase titer and yield. Only a few studies have been published that provide information on bioprocess development, with media optimization and fed-batch cultivation procedure being particularly promising areas.

A full genome assembly reveals drought stress effects on gene expression and metabolite profiles in blackcurrant ( L.).

Blackcurrant ( L., family Grossulariaceae) is a perennial shrub that is widely cultivated for its edible berries. These are rich in antioxidants, vitamin C, and anthocyanins, making them a valuable ingredient in the food and beverage industry.

Protolytic Reactions at Electrified TiO P25 Interface: Quantitative and Thermodynamic Characterization.

Protolytic reactions on the surface of a titania photocatalyst (TiO P25 containing chlorine impurities) were studied using potentiometric and calorimetric acid-base titration. The impurity was removed by either washing or heat treatment. The efficiency of purification was tested by chlorine (TOX) analysis and acid-base titration.

Upcycling of polyamides through chemical hydrolysis and engineered Pseudomonas putida.

Aliphatic polyamides, or nylons, are widely used in the textile and automotive industry due to their high durability and tensile strength, but recycling rates are below 5%. Chemical recycling of polyamides is possible but typically yields mixtures of monomers and oligomers which hinders downstream purification. Here, Pseudomonas putida KT2440 was engineered to metabolize C-polyamide monomers such as 6-aminohexanoic acid, ε-caprolactam and 1,6-hexamethylenediamine, guided by adaptive laboratory evolution.

Characterization of a C-methyltransferase from - crystal structure, mechanism, and substrate scope.

Despite the presence of pyrroloindoles in many natural products with diverse biological activities, their synthesis remains challenging in terms of stereoselectivity, especially with respect to methylation at the indole C3 position. In the present study, the pyrroloindole motif in tryptophan-based diketopiperazines (DKPs) is synthesized using the SAM-dependent methyltransferase SgMT from . The three-dimensional structure of this indole C3-methyltransferase was determined by X-ray crystallography, providing insights into the enzyme.

Engineered Passive Glucose Uptake in Pseudomonas taiwanensis VLB120 Increases Resource Efficiency for Bioproduction.

Glucose is the most abundant monosaccharide and a principal substrate in biotechnological production processes. In Pseudomonas, this sugar is either imported directly into the cytosol or first oxidised to gluconate in the periplasm. While gluconate is taken up via a proton-driven symporter, the import of glucose is mediated by an ABC-type transporter, and hence both require energy.

Cyclophilin A Regulates Tripartite Motif 5 Alpha Restriction of HIV-1.

The peptidyl-prolyl isomerase A (PPIA), also known as cyclophilin A (CYPA), is involved in multiple steps of the HIV-1 replication cycle. CYPA regulates the restriction of many host factors by interacting with the CYPA-binding loop on the HIV-1 capsid (CA) surface. TRIM5 (tripartite motif protein 5) in primates is a key species-specific restriction factor defining the HIV-1 pandemic.

Comparative Study of Crucial Properties of Packaging Based on Polylactide and Selected Essential Oils.

In order to establish the differences in packaging containing various essential oils, polylactide (PLA)-based polymeric films incorporating poly(ethylene glycol) (PEG), clove (C), grapefruit (G), rosemary (R), and tea tree (T) essential oils were obtained and subsequently analyzed. In addition to examining structure and morphology, the polymer films underwent analyses that are particularly important with regard to contact with food. Mechanical and antioxidant properties, water vapor transmission rate (WVTR), and analysis of barrier properties against ultraviolet (UV) radiation, as well as the migration of ingredients into food simulants such as 10% / solutions of ethanol, 3% / acetic acid solution, and isooctane, were among the critical studies conducted.

Insights into the morphology-productivity relationship of filamentous fungi through small-scale cultivation and automated microscopy of Thermothelomyces thermophilus.

Filamentous fungi are a cornerstone in the biotechnological production of enzymes, proteins, and organic acids. However, challenges in understanding and controlling the relationship between morphology and productivity can limit their application. This study addresses these challenges using Thermothelomyces thermophilus, a promising thermophilic fungus known for the production of thermostable enzymes.

Agrivoltaics shading enhanced the microclimate, photosynthesis, growth and yields of vigna radiata genotypes in tropical Nigeria.

In recent years, more agricultural lands are been converted to photovoltaic (PV) power plants for better return on investment. However, prioritizing energy generation over food production poses a significant threat to the well-being of the rapidly growing global population. Agro-photovoltaics (APV) provide an opportunity to integrate crop production under PV panels.

Novel 4-alkoxy Meriolin Congeners Potently Induce Apoptosis in Leukemia and Lymphoma Cells.

(3-(pyrimidin-4-yl)-7-azaindoles) are synthetic hybrids of the naturally occurring alkaloids and and display a strong cytotoxic potential. We have recently shown that the novel derivative is highly cytotoxic in several lymphoma and leukemia cell lines as well as in primary patient-derived lymphoma and leukemia cells and predominantly targets cyclin-dependent kinases (CDKs). Here, we efficiently synthesized nine novel 2-aminopyridyl congeners (-), i.

Phytochemical Study and In Vitro Antioxidant Activity of Along with Antitumor Activity of the Isolated -Tiliroside and Luteolin 4'--β-Xyloside.

Twelve compounds (-), kaempferol (), luteolin (), luteolin 4'--xyloside (), luteolin 4'--β-glucoside (), quercetin 4'--β-xyloside (), kaempferol-3--[6″--(E)-p-coumaroyl]-β-D-glucoside (-tiliroside) (), protocatechuic acid (), gallic acid (), methyl gallate (), ethyl gallate (), shikimic acid-3--gallate (), and 3,3',4'-tri--methyl-ellagic acid 4-sulfate (), were isolated and identified from the aerial parts of (Cav.) Pers (synonym: C. Presl.

CD63 as novel target for nanoemulsion-based F MRI imaging and drug delivery to activated cardiac fibroblasts.

Cardiac fibroblasts are activated following myocardial infarction (MI) and cardiac fibrosis is a major driver of the growing burden of heart failure. A non-invasive targeting method for activated cardiac fibroblasts would be advantageous because of their importance for imaging and therapy. Targeting was achieved by linking a 7-amino acid peptide (EP9) to a perfluorocarbon-containing nanoemulsion (PFC-NE) for visualization by F-combined with H-MRI.

Plant species determine elementome differentiation and element coupling during deadwood decay.

The elemental dynamics and interactions within deadwood profoundly influence carbon sequestration and nutrient cycling in forest ecosystems. Recent studies have investigated macronutrient cycling during deadwood decay of specific plants, yet the dynamics and interactions of micronutrients, trace elements, and the elementome across species and decay stages remain unexplored. Here, we investigated the elementome and their coupling relationships across five decay stages of downed deadwood (DDW) from four dominant species (Hippophae rhamnoides, Populus purdomii, Abies fabri, and Picea brachytyla) along the Hailuogou Glacier primary successional chronosequence.

Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesized proteins.

A comprehensive understanding of microbial community dynamics is fundamental to the advancement of environmental microbiology, human health, and biotechnology. Metaproteomics, defined as the analysis of all proteins present within a microbial community, provides insights into these complex systems. Microbial adaptation and activity depend to an important extent on newly synthesized proteins (nP), however, the distinction between nP and bulk proteins is challenging.

Concatenating Microbial, Enzymatic, and Organometallic Catalysis for Integrated Conversion of Renewable Carbon Sources.

The chemical industry can now seize the opportunity to improve the sustainability of its processes by replacing fossil carbon sources with renewable alternatives such as CO, biomass, and plastics, thereby thinking ahead and having a look into the future. For their conversion to intermediate and final products, different types of catalysts-microbial, enzymatic, and organometallic-can be applied. The first part of this review shows how these catalysts can work separately in parallel, each route with unique requirements and advantages.

Tire Wear Particles Exposure Enhances Denitrification in Soil by Enriching Labile DOM and Shaping the Microbial Community.

Tire wear particles (TWP) are emerging contaminants in the soil environment due to their widespread occurrence and potential threat to soil health. However, their impacts on soil biogeochemical processes remain unclear. Here, we investigated the effects of TWP at various doses and their leachate on soil respiration and denitrification using a robotized continuous-flow incubation system in upland soil.