Synergistic HRP/Biotin encapsulation in DNA nanoflowers via RCA: A path to stabilized, streamlined ELISA platform.

Background: Enzyme-linked immunosorbent assay (ELISA) requires the simultaneous maintenance of enzyme activity and the efficient labelling of the enzymes on the antibodies. Challenges in ELISA include the enzyme decomposition under harsh conditions and the requirement for secondary antibodies for binding. Preserving enzyme activity and simplifying antibody binding is a crucial prerequisite for expanding the applications of ELISA.

The detection of by aptamer-based qPCR.

The bacterium can infect a wide variety of fish species and is a common pathogen in aquaculture. Rapid and accurate detection of the pathogen is the premise and basis for its disease prevention and control. In this study, an aptamer with high affinity and specificity was used to bind The aptamers that bound to the pathogen were then separated and used as the templates for SYBR Green I real-time quantitative polymerase chain reaction (qPCR) amplification.

Surface engineering of 3D covalent organic frameworks via crystalline modulation for enhanced profiling of polychlorinated biphenyls.

Surface engineering of covalent organic frameworks (COFs) represents a highly promising strategy for achieving superior capture capacity towards environmental trace pollutants. Herein, the three-dimensional covalent organic frameworks (3D-COFs) with tunable crystallinity were synthesized and optimized via a simple solvent ratio adjustment, demonstrating an exceptional capture capacity of polychlorinated biphenyls (PCBs). By adjusting the ratio of solvents (1,4-dioxane and toluene), a functional 3D COF (dia-c5 TPA-TAM-COF) was discovered, providing an ideal approach to transform an amorphous compound into a functional platform with a significant increase in crystallinity, surface area and pore volume for PCBs capture.

Dual-mode monolithic column with zwitterion antifouling and aptamer affinity for online specific recognition of okadaic acid.

Developing a functional media for precise identification of trace shellfish toxin would underpin the effective assessment of marine pollution. Herein, a novel monolithic column with a dual-mode strategy integrating antifouling and aptamer bionic affinity recognition was proposed for online specific identification of the marine toxin okadaic acid (OA). The zwitterionic monomer 2-(methacryloyloxy)ethylphosphorylcholine (MPC) and aptamers were synergistically employed to enable efficient reduction of matrix interferences and selective capture of target OA.

Selection of biofilm-inhibiting ssDNA aptamers against antibiotic-resistant Edwardsiella tarda by inhibition-SELEX and interaction with their binding proteins.

Biofilms can increase bacterial resistance to antibiotic therapies. Edwardsiella tarda with biofilm is highly resistant to antibacterial treatment, especially for the antibiotic-resistant strain. In this study, we obtained biofilm-inhibiting aptamers against antibiotic-resistant E.

Transcriptomic analysis of the inhibition mechanisms against by antibacterial aptamer B4.

is a common bacterial pathogen in aquaculture, often leading to visceral white spot disease in large yellow croakers (). Previous studies have found that certain aptamers show an efficient antibacterial effect against this pathogen. In this study, we analyzed the transcriptome of to get insights into the antibacterial and inhibitions mechanisms following exposure to the aptamer B4.