Genetic profile of tuberculosis among the migrant population in Fujian Province, China.

Setting: The Fujian District in China has a high migrant worker population. Although tuberculosis (TB) among migrants is a serious threat to public health in Fujian, little is known about the molecular characteristics of TB isolates in this population.

Objective: To investigate the genetic profile of TB among the migrant population in Fujian.

[Immuno-affinity chromatographic purification: the study of methods to test citrinin in monascus products by high performance liquid chromatography].

Objective: To establish a method to test citrinin (CIT) in monascus products by immuno-affinity chromatography (IAC)-high performance liquid chromatography (HPLC), and to detect the content of CIT in monascus products in Fujian province.

Methods: IAC-HPLC was applied to detect the CIT content in monascus products. The conditions to use HPLC were as follows: C(18) reversed-phase chromatographic column, 150.

[Relationship between acute radiotoxicity and nutritional status of patients with head and neck cancer].

Objective: To investigate the relationship between nutritional status and acute radiotoxicity of patients with head and neck cancer.

Methods: Nutritional status and acute radiotoxicities of 130 head and neck cancer patients were prospectively surveyed during the 4th week of radiotherapy. Relationships between nutritional status and acute radiotoxicities were analyzed by canonical correlation.

[Surveillance of schistosomiasis in national surveillance sites of Fuqing City and Xiapu County, 2006 -2009].

Objective: To understand the status of national surveillance sites of schistosomiasis in Fuqing City and Xiapu County.

Methods: According to the National Scheme of Shistosomiasis Surveillance, the data of the surveillance and field survey in the national surveillance sites of schistosomiasis of Fuqing City and Xiapu County were collected and analyzed statistically from 2006 to 2009.

Results: Twenty residual Oncomelania snail points and 8 villages with snails were found in 36 surveillance sites.

[Effect of glucosamine sulfate on the immunological function of normal mice].

Objective: To observe the effects of glucosamine sulfate on the immunity of normal mice.

Methods: Female ICR mice were divided by weight into four groups, including low dose group (150 mg x kg(-1)), medium dose group (300 mg x kg(-1)), high dose group (900 mg x kg(-1)) and normal control group. Spleen and thymus were weighted 30 days later, cell mediated immunity, humoral immunity, phagocytosis of mononuclear phagocyte and the activity of NK cell were assayed.

[Sequencing and analysis of the complete genome sequence of WU polyomavirus in Fuzhou, China].

WU polyomavirus (WUPyV), a new member of the genus Polyomavirus in the family Polyomaviridae, is recently found in patients with respiratory tract infections. In our study, the complete genome of the two WUPyV isolates (FZ18, FZTF) were sequenced and deposited in GenBank (accession nos. FJ890981, FJ890982).

[Survey on the foci of Paragonimus in Youxi, Yongtai and Pinghe Counties of Fujian Province].

Objective: To investigate the natural foci of paragonimiasis in Youxi, Yongtai and Pinghe Counties of Fujian Province.

Methods: One village each from the three counties was selected according to reported paragonimiasis cases. Freshwater snails collected from fields were examined for cercariae.

[Two new species of freshwater crabs (Decapoda: Potamidae) serving as intermediate hosts of Paragonimus in Fujian, China].

Objective: To investigate the natural resources of the freshwater crab which can serve as the intermediate hosts of Paragonimus in Pinghe and Youxi of Fujian Province.

Methods: Freshwater crabs were collected. The morphological characteristics of the crabs and the habitats were observed.

[A new species of the genus Nanhaipotamon (Decapoda: Potamidae) from Xiapu County, Fujian].

A new species of Nanhaipotamon sp. Nov. was found from the west of Xiapu County, Fujian Province in April 2008.

Entertainment Venue Visiting and Commercial Sex in China.

Entertainment venues in China play an important role in the sexually transmitted disease (STD)/HIV epidemic. Most previous studies have focused on sex workers working in entertainment venues, but little is known about their clients. This study investigated the perceptions and behavior of the patrons visiting entertainment venues.

[A duplex nested PCR assay detecting of Vibrio cholerae and its application on environmental specimens].

Objective: To establish a duplex nested PCR assay system which is capable for detecting O1 and O139 groups of Vibrio cholerae simultaneously, and is applicable to environmental specimens from routine cholera surveillance.

Methods: Based on nucleic acid sequences available in GenBank, six sets of primers were designed by PrimerSelect program of DNAStar, targeting the rfb gene that encodes the O antigens of O1 and O139 V. cholerae, respectively.

Self-reported sexually transmitted disease symptoms and treatment-seeking behaviors in China.

In recent decades, sexually transmitted diseases (STDs) have reemerged and spread as a major public health problem in China. However, little effort has been made on promoting appropriate health-seeking behaviors among people living with STDs. A randomly selected sample of market vendors in Fuzhou (N = 4510) was recruited and assessed from 2003 to 2004 to examine their choice of pharmacy versus hospital, and folk remedy versus Western medicine when having STD symptoms.

[Analysis of the amino acid changes of the hemagglutinin of H5 avian influenza virus].

We introduced 38 single-point amino acid changes into the hemagglutinin (HA) protein of the reassortmented A/Duck/Mongolia/54/01 (H5N2) strain by a PCR random mutation method. The percentage of amino acid changes on the HA domain that did not abrogate hemadsorption activity was calculated to be 89%. Changes in the amino acids of the HA2 domain were observed to be about half of those in the HA1 domain of these mutants.

Identification and quantification of tetrodotoxin in the marine gastropod Nassarius by LC-MS.

Tetrodotoxin (TTX) has been usually analyzed in marine and other sources by HPLC separation, followed by alkaline treatment and fluorescence assay. However, the applications of such methods were limited because of their tedious extraction and derivation procedures. A simpler, rapid LC-MS method, using selected ion-monitoring, has now been developed, and used to identify and quantitate TTX in a marine gastropod of the genus Nassarius, collected from Fijian province of China.

[A sero-epidemiology study on hepatitis E virus infection in Fujian province].

Objective: To understand the sero-prevalence of hepatitis E virus (HEV) infection among different populations and animals in Fujian province.

Methods: One thousand one hundred and fifty-one serum samples were collected from 5 species of animals including swine, dog, cow, sheep and rat. A total of 2209 and 1722 serum samples from the general population and from the exposed population were collected.

High-level expression of recombinant dengue virus type 2 envelope domain III protein and induction of neutralizing antibodies in BALB/C mice.

Dengue fever is a growing public health problem in many countries since so far no effective vaccines are available. In this study, the domain III of dengue virus type 2 envelope was expressed in Escherichia coli without fusion of any carrier protein. The recombinant protein was detected in the form of inclusion bodies, which were solubilized in 8M urea and could be purified subsequently by high-performance liquid chromatography (HPLC) on an ion exchange column.

Genetic characterization of CRF01_AE full-length human immunodeficiency virus type 1 sequences from Fujian, China.

HIV-1 CRF01_AE is the main prevalent HIV-1 recombinant strain in China and the dominant strain in Fujian as well; therefore, obtaining and understanding the genetic characterization of this clade become important. To date only a few full-length HIV-1 CRF01_AE genomes from China have been sequenced. We reported 13 full-length HIV-1 CRF01_AE isolate sequences from Fujian, China.

[Review on the biological research of human Demodex].

This article summarizes the present researches on biology of human Demodex about the methods of examination, morphology of adult parasite, biological habits and characteristics, and in vitro survival.

[Molecular identification and genetic analysis of an echovirus 19-associated epidemic of aseptic encephalitis].

Objective: To ascertain the pathogen of aseptic encephalitis epidemic in Long-Yan city in Fujian, and to find out the genetic characteristics of the virus.

Methods: Rapid detection of enteroviral RNA by reverse transcription polymerasechain reaction (RT-PCR) was directly carried out in cerebrospinal fluid(CSF) to isolate and identify the viruses from CSF at the same time, and to detect the neutralization antibody in two serum specimens collected in acute and convalescence phase. Nucleotides of VP1 region was also analyzed by constructing phylogenetic tree.

[Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province].

Objective: To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene.

Methods: PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program.