A Reverse Vaccinology and Immunoinformatic Approach for the Designing of a Novel mRNA Vaccine Against Stomach Cancer Targeting the Potent Pathogenic Proteins of .

infection of the stomach's epithelial cells is a significant risk factor for stomach cancer. Various proteins (CagA, GGT, NapA, PatA, urease, and VacA) were targeted to design 2 messenger RNA (mRNA) vaccines, V1 and V2, using bioinformatics tools. Physicochemical parameters, secondary and tertiary structure, molecular docking and dynamic simulation, codon optimization, and RNA structure prediction have also been estimated for these developed vaccines.

Gut microbiota diversity affects fish behaviour and is influenced by host genetics and early rearing conditions.

The gut microbiota influences human and animal cognition and behaviour through its effects on the endocrine and immune systems. The microbiome-behaviour relationship may be especially relevant for fish, due to their diverse evolutionary history and potential implications for farming and conservation. Yet, there is limited research on the interaction between gut microbiome and behaviour in non-model fish.

Functional analysis of promoter element 2 within the viral polymerase gene of an emerging paramyxovirus, Sosuga virus.

Paramyxovirus genomes carry bipartite promoters at the 3' ends of both their genome and antigenome, thereby initiating RNA synthesis, which requires the viral polymerase to recognize two elements: the primary promoter element 1 (PE1) and the secondary promoter element 2 (PE2). We have previously shown that the antigenomic PE2 (agPE2) in many viruses in the subfamily is located within the coding region of the viral RNA polymerase L gene. Sosuga virus (SOSV), belonging to the subfamily, is highly pathogenic to humans, thus necessitating high-level containment facilities for infectious virus research.

Antioxidant, Anti-Inflammatory, and Neuropharmacological Potential of Syngonium podophyllum Flower Methanolic Extract: Insights From In Vivo, In Vitro, In Silico, and GC-MS/MS Analysis.

Syngonium (S.) podophyllum L. is recognized for its diverse applications.

Revolutionizing Chikungunya Vaccines: mRNA Breakthroughs With Molecular and Immune Simulations.

With the ability to cause massive epidemics that have consequences on millions of individuals globally, the Chikungunya virus (CHIKV) emerges as a severe menace. Developing an effective vaccine is urgent as no effective therapeutics are available for such viral infections. Therefore, we designed a novel mRNA vaccine against CHIKV with a combination of highly antigenic and potential MHC-I, MHC-II, and B-cell epitopes from the structural polyprotein.

Structural and Functional Characterization of a Putative Type VI Secretion System Protein in as a Potential Therapeutic Target: A Computational Study.

Background: , a foodborne pathogen with a fatality rate of 33%, is a rod-shaped, Gram-negative, non-spore-forming bacterium responsible for causing meningitis, bacteremia, and necrotizing enterocolitis. Despite many unknown functions of hypothetical proteins in bacterial genomes, bioinformatic techniques have successfully annotated their roles in various pathogens.

Objectives: The aim of this investigation is to identify and annotate the structural and functional properties of a hypothetical protein (HP) from 7G strain (accession no.

Detrimental effects of chronic arsenic exposure through daily diet on hepatic and renal health: An animal model study.

Background: Bangladesh is heavily impacted by arsenic contamination; however, in-depth research regarding the consequences of arsenic exposure through contaminated food remains in its nascent stage.

Objectives: Our study aimed to examine the effects of consuming arsenic-contaminated rice and vegetables on Wistar albino rats.

Methodology: A total of 30 Wistar albino rats were divided into control and experimental groups, receiving diets containing arsenic-contaminated rice and vegetable for 120 days.

Optimization of Split Feeding Strategy for Laying Hens Through a Response Surface Model.

Laying hens' metabolism goes through a cyclic process to produce eggs, which requires higher dietary protein and energy in the morning (AM) and higher calcium (Ca) in the afternoon/evening (PM) than the rest of the day. Therefore, poultry scientists are trying to adopt a new feeding strategy called AM/PM or split feeding to precisely meet hen's requirements more effectively than conventional methods. A 10-week cage layer trial was carried out via a Box-Behnken response surface design to identify the optimal amount of protein, energy, and calcium of the AM/PM diets.

Afternoon Calcium and Vitamin D Supplementation in Water: A Targeted Approach to Improve Laying Hen Nutrition.

A modified AM/PM strategy was evaluated in this study by supplementing calcium (Ca) in afternoon/evening (PM) drinking water instead of feed, offering an alternative to supplying two diets. A 10-week trial was conducted with 288 Hy-Line Brown hens (56 weeks old) housed on the floor in 18 pens (16 hens per pen; 9 replicate pens per treatment). The control diet contained 4.

Draft genome sequences of four lactic acid bacteria from fermented chicken meat unveil biosynthetic gene clusters for antimicrobial compounds.

Lactic acid bacteria play a crucial role in fermented food production and serve as important sources of antimicrobial peptides. This study reports four lactic acid bacteria strains isolated from fermented chicken meat, which harbor biosynthetic gene clusters encoding antimicrobial compounds. These strains are classified within the genera and .

Isolation and antimicrobial resistance patterns of and from caprine respiratory tract infections: A hospital-based clinical study.

Objective: and are the most common opportunistic pathogens frequently associated with respiratory tract infection (RTI) in different animals. This cross-sectional study aimed to identify the occurrence of and in goats with RTI, analyze the antimicrobial resistance patterns, and explore potential risk factors contributing to RTI.

Materials And Methods: A total of 120 nasal swab samples were collected from goats, and standard classical bacteriological methods were performed to isolate and identify and .

Exopolysaccharides from camel milk-derived C66: Structural characterization, bioactive and rheological properties for food applications.

Microbial exopolysaccharides (EPS) are valued as safe, functional bio-ingredients in food. Produced by microorganisms like lactic acid bacteria (LAB), they enhance the rheological and sensory properties of food products. In this study, C66 (EPS-C66), was utilized to produce EPS.

University Students' Sociodemographics, Physical Inactivity, and Inadequate and Poor-Quality Sleep Are Associated With Their Overweight/Obesity: Findings From a Case-Control Study in Bangladesh.

Background And Aims: Evidence confirms a high prevalence of general and abdominal obesity among university students in Bangladesh. The primary objective of this case-control study was to examine the association between overweight/obesity and sleep patterns (including sleep duration and quality) among university students in Bangladesh. The secondary objective was to identify the sociodemographic and lifestyle-related factors that predict overweight/obesity in the study population.

Growth Performance, Dietary Enzyme Profiling, and Antioxidant-Induced Immunity of Fed With Microalgal Diet.

Diseases, overfishing, and habitat loss are constantly reducing oyster populations. Moreover, environmental pollution and natural disasters also hinder offshore shellfish cultivation. Therefore, filter-feeding bivalves could be cultivated on land-based system to maintain the culture condition and secure food safety.

Designing of a multiepitope-based vaccine against echinococcosis utilizing the potent Ag5 antigen: Immunoinformatics and simulation approaches.

Echinococcosis is a significant parasitic zoonotic disease with severe implications for human and animal health. To date, there has been no effective vaccine candidate available for echinococcosis. Therefore, we employed computational approaches to develop a multiepitope-based vaccine using the most potent epitopes of MHC-I, MHC-II, and B-cell derived from the Ag5 protein of Echinococcus spp.

Phytoplankton in contrasting ecosystems of the southeastern coast of Bangladesh: effects of seasonality and environmental factors.

Phytoplankton forms the foundation of the aquatic food chain, with their community composition dependent on environmental conditions. Research on phytoplankton communities in relation to environmental variables across diverse coastal ecosystems in Bangladesh remains limited and focusing on small or discrete areas. This study aimed to explore phytoplankton dynamics across contrasting ecosystems characterized by varying salinity levels (ranging from 0 to 36 ppt), encompassing riverine to marine ecosystem.

Cadmium Pollution Deteriorates the Muscle Quality of Labeo rohita by Altering Its Nutrients and Intestinal Microbiota Diversity.

The detrimental effects of cadmium (Cd), a hazardous heavy metal, on fish have triggered global concerns. While the ecotoxicity of Cd on fish has been investigated, the impact of Cd on muscle quality and its correlation with the gut microbiota in fish remains scarce. To comprehensively uncover Cd effects based on preliminary muscle Cd deposition, relevant studies, and ecological Cd pollution data, we exposed Labeo rohita to Cd under concentrations of 0.

The Global Burden of Emerging and Re-Emerging Orbiviruses in Livestock: An Emphasis on Bluetongue Virus and Epizootic Hemorrhagic Disease Virus.

Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are vector-borne orbiviruses that pose an emerging threat to livestock, including cattle and sheep. This review summarizes the global distribution, genetic diversity, and key factors driving their spread along with the existing knowledge gaps and recommendations to mitigate their impact. Both viruses cause hemorrhagic disease in susceptible ruminants and are commonly reported in tropical and subtropical regions including North America, Asia, Africa, Oceania, and some parts of Europe.

Lactococcus lactis subsp. lactis boosts stress resistance and host defense mechanisms in Caenorhabditis elegans.

Aims: To investigate the effects of Lactococcus lactis subsp. lactis strains LL100933 and LL12007 on the host defense mechanisms of Caenorhabditis elegans against pathogenic infections and stressors.

Methods And Results: Caenorhabditis elegans fed a 1:1 mixture of Escherichia coli OP50 and LL100933 (OP50 + LL100933) or E.

Epidemiology, transmission dynamics, risk factors, and future directions of rabies in the Arabian Peninsula using one health approach: a review.

Despite global initiatives to eliminate dog-mediated human rabies by 2030, the Arabian Peninsula faces challenges due to insufficient data. This review addresses the current rabies situation and knowledge gaps in the region and proposes One Health interventions. Employing a mixed-method approach combining scoping and systematic review, the study commenced with a Delphi discussion to identify knowledge gaps and set objectives.

Detection and Characterisation of Colistin-Resistant in Broiler Meats.

The irrational use of antimicrobials has led to the emergence of resistance, impacting not only pathogenic bacteria but also commensal bacteria. Resistance against colistin, a last-resort antibiotic, mediated by globally disseminated plasmid-borne mobile colistin resistance () genes, has raised significant global concerns. This cross-sectional study aimed to investigate the antimicrobial resistance patterns of colistin-resistant () and mobilised colistin resistance ( 1-5) genes from broiler meat.

Designing of an mRNA vaccine against high-risk human papillomavirus targeting the E6 and E7 oncoproteins exploiting immunoinformatics and dynamic simulation.

Human papillomavirus 16 and human papillomavirus 18 have been associated with different life-threatening cancers, including cervical, lung, penal, vulval, vaginal, anal, and oropharyngeal cancers, while cervical cancer is the most prominent one. Several research studies have suggested that the oncoproteins E6 and E7 are the leading cause of cancers associated with the human papillomavirus infection. Therefore, we developed two mRNA vaccines (V1 and V2) targeting these oncoproteins.

Legume intakes on cardiometabolic profile and gut microbiome function: systematic review and meta-analyses of randomised controlled trials.

Background: Legumes are widely considered one of the most beneficial food groups to consume. They are high in fibre and plant-based protein as well as naturally low in sodium, saturated fats, and sugars. However, legumes do not feature prominently in the modern diet, and previous evidence syntheses show inconsistent results on cardiometabolic risk profile when increasing legume intakes.