Linking oxidative DNA lesion 8-OxoG to tumor development and progression.

Cells of the aerobic metabolic organism are inevitably subjected to the damage from reactive oxygen species (ROS). ROS cause multiple forms of DNA damage, among which the oxidation product of guanine G 8-hydroxyguanine (8-oxoG) is the most frequent DNA oxidative damage, recognized by the specific glycosidase OGG1 that initiates the base excision repair pathway. If left unrepaired, 8-oxoG may pair with A instead of C, leading to a mutation of G: C to T: A during replication.

[Improvement of the rapid identification method for H1 and H2 genotype of measles virus].

Objective: To simplify the procesure of the rapid genotyping method of H1 and H2 genotypes of measles virus and make the identifying operation easily.

Methods: To improve the reverse transcription nested-polymerase chain reaction (RT-nPCR) to RT-PCR by using the inner primer pair of the RT-nPCR, identify the genotype of the PCR product by using restriction fragment length polymorphism (RFLP) method, and also use the safer GeneFinder in electrophoresis instead of EB which maybe induce cancer.

Results: 3 strains measles virus of known H1 genotype got good positive results by the improved one-step RT-PCR and the PCR products were also cut well by Sal I.

[The establishment of method for identifying China vaccine strains and wild strains of measles virus].

Objective: To establish a simple and quick method for identifying China vaccine strains and wild strains of Measles Virus.

Methods: To search the enzyme site in Hemagglutinin gene of measles virus for different domestic vaccine strains and wild strains of measles virus, and design the RT-PCR primer within the range covering the enzyme site, and then to confirm the specificity and sensibility of the RT-PCR method, and then identify the RT-PCR product by RFLP.

Results: The one-step RT-PCR method is sensitive, the measles virus of 4.