Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
During embryonic development, neural crest-derived melanoblasts, which are precursors of pigment-producing melanocytes, disperse throughout the skin by long-range cell migration that requires adhesion to the ECM. Members of the integrin family of cell-ECM adhesion receptors are thought to contribute to melanocyte migration . However, due to the functional redundancy between different integrin heterodimers, the precise role of integrins in melanoblast migration, as well as the mechanisms that regulate them in this process, especially in contexts, remain poorly understood. To address this, we utilize the existing transcriptomic databases to identify different integrin subunits that are specifically expressed in melanoblasts, melanocytes and melanoma cancer cell lines. We then use mouse embryonic skin explants combined with drug and small molecule-based perturbations to target different integrins as well as specific mechanisms that modulate integrin activity. Individual melanoblasts from live imaging movies are tracked using high-resolution, quantitative, automated analysis, and cell morphology, cell migration and actin-based protrusions are analyzed. Overall, we uncover the non-redundant roles of different integrin heterodimers and elucidate the function of outside-in integrin activation in melanoblasts. Finally, we describe the function played, by integrin-mediated adhesion to specific ECM ligands during melanoblast migration. [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text].
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1091/mbc.E25-02-0066 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Defining the Role of Integrins in Melanoblast Migration .
Mol Biol Cell
September 2025
Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
During embryonic development, neural crest-derived melanoblasts, which are precursors of pigment-producing melanocytes, disperse throughout the skin by long-range cell migration that requires adhesion to the ECM. Members of the integrin family of cell-ECM adhesion receptors are thought to contribute to melanocyte migration . However, due to the functional redundancy between different integrin heterodimers, the precise role of integrins in melanoblast migration, as well as the mechanisms that regulate them in this process, especially in contexts, remain poorly understood.
View Article and Find Full Text PDFLoss of CHMP2A implicates an ordered assembly of ESCRT-III proteins during cytokinetic abscission.
Mol Biol Cell
September 2025
Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva 84105, Israel.
The ESCRT machinery mediates membrane remodeling in fundamental cellular processes including cytokinesis, endosomal sorting, nuclear envelope reformation, and membrane repair. Membrane constriction and scission is driven by the filament-forming ESCRT-III complex and the AAA-ATPase VPS4. While ESCRT-III-driven membrane scission is generally established, the mechanisms governing the assembly and coordination of its twelve mammalian isoforms in cells remain poorly understood.
View Article and Find Full Text PDFIntegrating clinical anxiety scales with pre-trained language models for anxiety recognition on social media.
Health Inf Sci Syst
December 2025
Gansu Provincial Key Laboratory of Wearable Computing, School of Information Science and Engineering, Lanzhou University, Lanzhou, 730000 China.
Leveraging natural language processing to identify anxiety states from social media has been widely studied. However, existing research lacks deep user-level semantic modeling and effective anxiety feature extraction. Additionally, the absence of clinical domain knowledge in current models limits their interpretability and medical relevance.
View Article and Find Full Text PDFDigital Hostility Toward LGBTQIA+ Research Recruitment on Social Media Using Topic Modeling and Sentiment Analysis of Facebook Comments: Quantitative Content Analysis Study.
J Med Internet Res
September 2025
Department of Psychological and Brain Sciences, Boston University, Boston, United States.
Background: Lesbian, gay, bisexual, transgender, queer/questioning, intersex, asexual (LGBTQIA+) researchers and participants frequently encounter hostility in virtual environments, particularly on social media platforms where public commentary on research advertisements can foster stigmatization. Despite a growing body of work on researcher virtual hostility, little empirical research has examined the actual content and emotional tone of public responses to LGBTQIA+-focused research recruitment.
Objective: This study aimed to analyze the thematic patterns and sentiment of social media comments directed at LGBTQIA+ research recruitment advertisements, in order to better understand how virtual stigma is communicated and how it may impact both researchers and potential participants.
Machine Learning and Lexical Rule-Based Cost-Efficient Emotion Annotation of Hinglish Utterances.
J Vis Exp
August 2025
Chitkara University Institute of Engineering & Technology, Chitkara University.
Emotion annotation in code-mixed languages like Hinglish (Hindi-English) presents unique challenges due to linguistic complexity and resource constraints. This study introduces a hybrid active learning framework that combines lexical rules, machine learning, and iterative expert feedback to achieve cost-efficient, high-accuracy emotion annotation. Grounded in psychological theories of emotion, including Discrete Emotions Theory and Cognitive Appraisal Theory, the framework employs bilingual emotion dictionaries (e.
View Article and Find Full Text PDF