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Article Abstract
Background: Nucleic Acid Amplification Tests (NAAT) remain one of the most reliable methods for pathogen identification. Given the high false-negative rates associated with traditional staining and microscopic examination, the time-consuming nature and low sensitivity of bacterial culture methods, as well as the inability of conventional NAAT to achieve absolute quantification.
Methods: To achieve rapid and quantitative detection of , we selected the 23S rRNA gene as the target for identification and developed a droplet digital PCR detection method.
Results: The entire detection process can be completed within 92 min, demonstrating high efficiency. The sensitivity reached 4.4 pg/μL, and no positive droplets were detected in experiments involving eight negative control pathogens, confirming high specificity. Additionally, the ddPCR assay for exhibited excellent repeatability, with a calculated coefficient of variation of 1 %.
Conclusion: The ddPCR detection technology demonstrates characteristics such as absolute quantification, high sensitivity, high specificity, and high reproducibility for , showing promise as an excellent testing platform. This advancement could provide a more scientific basis for clinical diagnosis and treatment.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12410466 | PMC |
| http://dx.doi.org/10.1016/j.plabm.2025.e00499 | DOI Listing |
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