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Introduction: To compensate for inadequate clinical exposure to temporomandibular disorders (TMD) among dental students during predoctoral training, a virtual patient-based learning (VPBL) platform was developed for integration into the TMD predoctoral curriculum. This study tested the VPBL's content validity among 10 orofacial pain (OFP) experts responsible for TMD education at U.S. institutions.
Methods: We developed a digital platform of 20 virtual patients (VPs) using Articulate Storyline, featuring common OFP conditions (based on Diagnostic Criteria for TMD) and divided into four modules (muscular-TMD, articular-TMD, mixed-TMD, and non-TMD). Each OFP-expert was randomly assigned to one VP from each module (a total of 4 VPs). VP's content validity was assessed through the Suitability Assessment of Materials across 6 domains (content, literacy level, graphics, layout, learning simulation, and cultural appropriateness) by computing Item-Level Content Validity Index (0-1 I-CVI, with ≥0.78 = "excellent validity" based on established recommendations), Scale-CVI/Universal Agreement (0-1 S-CVI/UA, with ≥0.8 = "excellent"), and total suitability score (0%-100%, with ≥70% = "superior material").
Results: The total suitability score was 89.1%±5.1 (range 81.2%-93.2%), corresponding to "superior material." The average VP's CVI was 0.95±0.01. The literacy level category received the lowest scores due to >9th-grade reading level demand (I-CVI 0.28±0.38, range 0.22-0.33). The S-CVI/UA was 0.93±0.04 (range 0.86-1.00) across all VPs, the lowest being a case of disc displacement without reduction in the articular TMD module (average I-CVI = 0.86).
Conclusions: The VPBL platform, designed for predoctoral TMD education, demonstrated excellent content validity among OFP experts. Future steps include testing its implementation in the TMD curriculum of a large predoctoral school.
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http://dx.doi.org/10.1002/jdd.70032 | DOI Listing |
J Sep Sci
September 2025
Programa De Pós-Graduação em Química, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil.
Secondary metabolites are important bioactive compounds for diet and medicine. This study optimizes the extraction of hydroethanolic herbal extracts using an EDGE (Energized Dispersive Guided Extraction) system, evaluates their antioxidant capacity, and analyzes correlations among antioxidant activity, total phenolic content, and individual compounds. A Doehlert matrix design was used to optimize extraction, having temperature and time as independent variables, and total phenolic content (mg GAE/g) as the response, quantified via the Folin-Ciocalteu method.
View Article and Find Full Text PDFBMC Med Educ
September 2025
School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Theor Appl Genet
September 2025
Institute for Breeding Research on Agricultural Crops, Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Sanitz, 18190, Germany.
Low-cost and high-throughput RNA sequencing data for barley RILs achieved GP performance comparable to or better than traditional SNP array datasets when combined with parental whole-genome sequencing SNP data. The field of genomic selection (GS) is advancing rapidly on many fronts including the utilization of multi-omics datasets with the goal of increasing prediction ability and becoming an integral part of an increasing number of breeding programs ensuring future food security. In this study, we used RNA sequencing (RNA-Seq) data to perform genomic prediction (GP) on three related barley RIL populations.
View Article and Find Full Text PDFOncogene
September 2025
Department of Breast Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Cholesterol biosynthesis is more activated in triple negative breast cancer (TNBC) than in other subtype breast cancer and plays essential role in facilitating TNBC. However, the regulatory network and how cholesterol biosynthesis contribute to TNBC development and progression are not well elucidated. Here, we found that reticulum membrane protein complex 2 (EMC2) is highly expressed in TNBC and predicts short survival of patients.
View Article and Find Full Text PDFMethods Cell Biol
September 2025
LR18ES03 Laboratory of Neurophysiology, Cellular Physiopathology and Valorisation of Biomolecules, Faculty of Science of Tunis, University Tunis El Manar, Tunis, Tunisia. Electronic address:
Binge drinking (BD) is a widespread pattern of excessive alcohol consumption among adolescents and young adults with detrimental consequences for brain development. Animal models are essential for investigating the neurobiological mechanisms underlying BD, but selecting an appropriate model is critical to ensure relevance to human behavior. This study aims to validate a murine model of (BD) using Swiss Webster mice.
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