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Fish are a vital aquatic resource worldwide, and the sustainable development of aquaculture is essential for global food security and economic growth. However, the high incidence of fish diseases in complex aquaculture environments significantly hampers sustainability, and traditional manual diagnosis methods are inefficient and often inaccurate. To address the challenges of small-lesion detection, lesion area size and morphological variation, and background complexity, we propose YOLO-TPS, a high-precision fish-disease detection model based on an improved YOLOv11n architecture. The model integrates a multi-module synergy strategy and a triple-attention mechanism to enhance detection performance. Specifically, the SPPF_TSFA module is introduced into the backbone to fuse spatial, channel, and neuron-level attention for better multi-scale feature extraction of early-stage lesions. A PC_Shuffleblock module incorporating asymmetric pinwheel-shaped convolutions is embedded in the detection head to improve spatial awareness and texture modeling under complex visual conditions. Additionally, a scale-aware dynamic intersection over union (SDIoU) loss function was designed to accommodate changes in the scale and morphology of lesions at different stages of the disease. Experimental results on a dataset comprising 4596 images across six fish-disease categories demonstrate superior performance (mAP: 97.2%, Precision: 97.9%, Recall: 95.1%) compared to the baseline. This study offers a robust, scalable solution for intelligent fish-disease diagnosis and has promising implications for sustainable aquaculture and animal health monitoring.
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http://dx.doi.org/10.3390/ani15162356 | DOI Listing |
Animals (Basel)
August 2025
College of Information and Intelligence, Hunan Agricultural University, Changsha 410128, China.
Fish are a vital aquatic resource worldwide, and the sustainable development of aquaculture is essential for global food security and economic growth. However, the high incidence of fish diseases in complex aquaculture environments significantly hampers sustainability, and traditional manual diagnosis methods are inefficient and often inaccurate. To address the challenges of small-lesion detection, lesion area size and morphological variation, and background complexity, we propose YOLO-TPS, a high-precision fish-disease detection model based on an improved YOLOv11n architecture.
View Article and Find Full Text PDFAquat Toxicol
October 2025
Faculty of Pharmaceutical Science, Assam Down Town University, Sankar Madhab Path, GandhiNagar, Panikhaiti, Guwahati, Assam 781026, India. Electronic address:
The widespread use of antibiotics in aquaculture has led to significant concerns regarding their presence in aquatic environments, their bioaccumulation in fish, and their potential toxicity to both aquatic life and human consumers. Antibiotics are extensively utilized to prevent and treat bacterial infections in farmed fish, but their residues have been detected in fish tissues, water bodies, and sediments. These residues contribute to antibiotic resistance, disrupt microbial ecosystems, and pose health risks upon consumption.
View Article and Find Full Text PDFJ Adv Res
July 2025
NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, the First Affiliated Hospital, Hainan Medical University, Haikou, Hainan 571199, China. Electronic address:
Introduction: Strategic investment in new interventions is crucial for controlling and eliminating NTDs. However, selecting the optimal intervention combination is challenging, especially with limited resources and urgent disease transmission. Hybrid models offer a flexible framework for simulating intervention scenarios, but their use in NTDs control remains underexplored.
View Article and Find Full Text PDFJ Fish Dis
May 2025
National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, People's Republic of China.
Cyprinid herpesvirus 2 (CyHV-2) poses a substantial global threat to goldfish (Carassius auratus) and crucian carp (Carassius carassius). Despite the development of several sensitive molecular diagnostic techniques, there is an ongoing demand for alternative visualisation platforms to streamline the workflow, enhance safety profiles, and improve accessibility for end-users. In this study, we have integrated recombinase-aided amplification (RAA) technology with the CRISPR/Cas12a system to establish a rapid diagnostic system for CyHV-2, termed one-pot RAA-CRISPR/Cas12a.
View Article and Find Full Text PDFBiol Open
September 2024
Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA 02129, USA and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
Here, we introduce 'TICIT', targeted integration by CRISPR-Cas9 and integrase technologies, which utilizes the site-specific DNA recombinase - phiC31 integrase - to insert large DNA fragments into CRISPR-Cas9 target loci. This technique, which relies on first knocking in a 39-basepair phiC31 landing site via CRISPR-Cas9, enables researchers to repeatedly perform site-specific transgenesis at the exact genomic location with high precision and efficiency. We applied this approach to devise a method for the instantaneous determination of a zebrafish's genotype simply by examining its color.
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