Comparative Effects of Maturity and Processing on Chemical Composition and Bioactivities in Leaves.

Foods

Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine of Anhui Province, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China.

Published: August 2025


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Article Abstract

("Heiyouchun") is a traditional Chinese woody vegetable, the leaves of which can also be processed into tea, known for its distinctive flavor and diverse bioactivities. However, the effects of leaf maturity and processing methods on its phytochemical composition and functional properties remain unclear. In this study, metabolomic analysis revealed 35 significantly different metabolites between tender and mature leaves, with higher concentrations of flavonoids, flavonoid glycosides, limonoids, and amino acids in tender leaves. Additionally, comparative analysis revealed that black tea fermentation preserves bioactive compounds more effectively than hot-air drying, particularly in tender leaves. In vitro activity assays showed that toon leaf tea extracts exhibited significant antioxidant and hypoglycemic effects, with black tea fermented tender leaves displaying the most potent bioactivity. Correlation analysis further confirmed a strong positive relationship between flavonoid/polyphenol content and bioactivity. These findings provide a theoretical foundation for optimizing processing techniques to enhance the functional properties of toon leaf tea.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12346186PMC
http://dx.doi.org/10.3390/foods14152717DOI Listing

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Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine of Anhui Province, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China.

("Heiyouchun") is a traditional Chinese woody vegetable, the leaves of which can also be processed into tea, known for its distinctive flavor and diverse bioactivities. However, the effects of leaf maturity and processing methods on its phytochemical composition and functional properties remain unclear. In this study, metabolomic analysis revealed 35 significantly different metabolites between tender and mature leaves, with higher concentrations of flavonoids, flavonoid glycosides, limonoids, and amino acids in tender leaves.

View Article and Find Full Text PDF