Genome-Wide Characterization of the Gene Family in and Homologous Validation of as a Central Regulator in Stress-Responsive Transcriptional Networks.

SnRK kinases, central regulators of plant stress response, remain uncharacterized in -an ancient gymnosperm valued for paclitaxel production. This study aimed to identify the family and elucidate its functional roles. Specifically, we identified genes through genomic analysis and assessed tissue-specific expression via transcriptomics, while regulatory networks were deciphered using WGCNA. To overcome experimental constraints, a PEG-mediated protoplast transient expression system was developed using calli, followed by dual-luciferase assays. Consequently, 19 genes (2 , 4 , 13 ) were identified, with tissue-specific expression revealing upregulation under methyl jasmonate (MeJA) and in stress-resilient tissues (bark/root). Subsequently, WGCNA uncovered a bark/root-specific module containing with predicted TF interactions (/). Critically, homologous dual-luciferase assays demonstrated activates and promoters (4.34-fold and 3.11-fold induction, respectively). This study establishes the family and identifies as a hub integrating stress signals (e.g., MeJA) to modulate downstream TF networks, while the novel protoplast system enables future functional studies in this medicinal plant.