Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The mechanism by which ohmic heating (OH) accelerates bacterial spore killing compared to conventional heating (CH) is unclear. This study used genetically modified Bacillus subtilis spores to investigate OH's impact on specific components. Flow cytometry assessed membrane integrity, and molecular dynamics (MD) simulations examined the DNA-SASP complex under an electric field. Among the inner membrane (IM) proteins (YetF, YdfS, and YkjA) tested for its resistance against OH and CH, YeTF was found to be the most significant contributor to spore resistance for both treatments. SASP, SpoVA proteins, and Ca-DPA interacted with the field, showing specific effects at certain temperature and field intensity combinations. Flow cytometry showed spore staining with propidium iodide (PI), which increased with higher field intensities, indicating significant IM damage. MD simulations showed that the electric field caused the SASP-DNA complex to dissociate, with greater separation at higher field intensities. Thus, OH accelerates spore killing by affecting key IM and core molecules.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12332034 | PMC |
| http://dx.doi.org/10.1038/s41538-025-00537-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Synthetic niclosamide-loaded controlled-release nanospheres with high solubility and stability exerting multiple effects against .
Front Microbiol
July 2025
School of Laboratory of Medicine, Hangzhou Medical College, Hangzhou, Zhejiang, China.
Introduction: Niclosamide (NIC) has significant potential as a clinical therapeutic agent for infection (CDI); however, its strong hydrophobicity hampers its oral bioavailability, and its active effects against remain unclear.
Methods: Niclosamide-loaded controlled-release hyaluronic acid-modified poly (lactic--glycolic acid) naosphernes (NIC@PLGA-HAs) were synthesized using an oil-in-water emulsion technique and their effects on cell growth, spore germination, biofilm formation, and NIC interaction sites with toxin B (TcdB) were analyzed.
Results: NIC@PLGA-HAs exhibited enhanced solubility and stability, with a water contact angle on a hydrophilic surface of 65.
Macrophage vacuolar ATPase (v-ATPase) function controls germination and hyphal growth independent of spore killing.
bioRxiv
July 2025
Department of Biological Sciences, Eukaryotic Pathogens Innovation Center, Clemson University, Clemson, South Carolina, 29634.
Tissue-resident macrophages efficiently internalize spores, forming a critical first line of defense against infection. However, the mechanisms that these cells use to control spores in vivo remain incompletely defined. Here, we used the live imaging capabilities of the larval zebrafish host model to assess the role of the v-ATPase complex in macrophage-mediated defense against in a whole vertebrate animal.
View Article and Find Full Text PDFPathways for accelerated bacterial spore killing with ohmic heating.
NPJ Sci Food
August 2025
The Ohio State University, Columbus, OH, USA.
The mechanism by which ohmic heating (OH) accelerates bacterial spore killing compared to conventional heating (CH) is unclear. This study used genetically modified Bacillus subtilis spores to investigate OH's impact on specific components. Flow cytometry assessed membrane integrity, and molecular dynamics (MD) simulations examined the DNA-SASP complex under an electric field.
View Article and Find Full Text PDFImmune priming of honey bees protects against a major microsporidian pathogen.
Pest Manag Sci
August 2025
Department of Entomology, Michigan State University, East Lansing, MI, USA.
Background: Honey bees face significant threats from pathogens like Nosema ceranae, a microsporidian parasite that contributes to global colony declines. Immune priming, exposure to pathogen antigens to stimulate protective responses, could mitigate infection risks. We tested whether priming honey bee larvae and adults with heat-killed N.
View Article and Find Full Text PDFCombination of Medium-High-Hydrostatic-Pressure Treatment with Post-/Pre-Heat Treatment for Pasteurization of Spore Suspended in Soy Milk.
Microorganisms
June 2025
Department of Food Production Science, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790-8566, Ehime, Japan.
Medium-high-hydrostatic-pressure (MHHP) treatment can induce the spore to germinate via activating the germination receptor, subsequently resulting in the loss of the heat resistance of the spore and finally killing the germinated spore, although the ungerminated spore, even after MHHP treatment, can survive. This study aims to clarify the pasteurization effect of the combination of MHHP treatment with post-/pre-heating treatment on spores suspended in soy milk as a food model. Regarding the results, the D value, as a known heat resistance indicator of the MHHP-treated spore, decreased in comparison with the untreated spore.
View Article and Find Full Text PDF