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Microbial spoiling and oxidative rancidity pose serious problems for the shelf life of Iranian traditional butter. In order to preserve butter, this study created zein-based electrospun nanofibers (NFs) that contain thyme essential oil (TEO), grape leaf extract (GLE: 5-30 %), and zinc oxide nanoparticles (ZnONPs). The ideal formulation (Zein/ZnONPs/GLE30 %/TEO) demonstrated good antibacterial activity (11 mm and 16 mm inhibition zones against S. aureus and E. coli), high hydrophobicity (137.5° contact angle), uniform morphology (638 ± 164.69 nm diameter), and antioxidant capacity (34 % DPPH scavenging). The NFs successfully increased the shelf life of butter packaging by reducing oxidation (peroxide values were much lower than control), inhibiting microbial growth (lower yeast/mold counts), and preserving physicochemical stability over a 30-day storage period. The results show that the NFs have a great deal of potential to improve the preservation of conventional dairy products using active packaging technology.
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http://dx.doi.org/10.1016/j.foodchem.2025.145399 | DOI Listing |
Food Chem
November 2025
Department of Green Technologies in Food Processing, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran. Electronic address:
Microbial spoiling and oxidative rancidity pose serious problems for the shelf life of Iranian traditional butter. In order to preserve butter, this study created zein-based electrospun nanofibers (NFs) that contain thyme essential oil (TEO), grape leaf extract (GLE: 5-30 %), and zinc oxide nanoparticles (ZnONPs). The ideal formulation (Zein/ZnONPs/GLE30 %/TEO) demonstrated good antibacterial activity (11 mm and 16 mm inhibition zones against S.
View Article and Find Full Text PDFMolecules
May 2025
LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
Vitamin E is widely used in cosmetics and dermatological applications for its antioxidant, anti-inflammatory, and healing properties, yet its industrial use is limited by poor stability and bioavailability. To address these challenges, this study developed zein-based microstructures encapsulating vitamin E using electrohydrodynamic (EHD) techniques and evaluated how zein concentration affects morphology and release behavior. The SEM analysis showed that biopolymer (zein) concentration significantly affects microstructure morphology.
View Article and Find Full Text PDFSci Rep
April 2025
Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Cell adhesion and proliferation of zein-based scaffolds in tissue engineering are restricted due to hydrophobicity and low surface energy, and they are not appropriate for cell culture. Polycaprolactone (PCL) and zein are two distinct polymers in terms of origin and function; they are synthetic and natural polymers, respectively. In addition, PCL and zein have hydrophobic and amphiphilic structures.
View Article and Find Full Text PDFInt J Biol Macromol
May 2025
Center of Chemical, Pharmaceutical and Food Sciences, Innovation and Solutions in Chemistry Laboratory, Federal University of Pelotas, Eliseu Maciel St., s/n, Pelotas, RS 96160-000, Brazil; Postgraduate Program in Biotechnology, Federal University of Pelotas, Eliseu Maciel St., s/n, Pelotas, RS 9616
Ultrafine fibers are gaining attention for their unique properties and wide-ranging applications. Among production techniques, electrospinning stands out for its cost-effectiveness, simplicity, and control over fiber structure and diameter. This study reports for the first time the development of ultrafine fibers using zein, a renewable protein, with the addition of fucoidan, a bioactive polysaccharide from brown algae, through electrospinning.
View Article and Find Full Text PDFInt J Biol Macromol
November 2024
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing 314102, China. Electronic address:
In this study, the electrospun core-shell nanofibers of zein/pullulan stabilized bilayer emulsions before and after genipin crosslinking were fabricated. The experimental results indicated that the addition of pullulan increased the apparent viscosity and elastic modulus of the bilayer emulsions, which was further increased after the chemical crosslinking of genipin. The nanofiber diameter increased from 102.
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